Status
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Gene information
General information
TC008263 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 7487406..7498377
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008263
    iBeetle screen iB_02771

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008263 RA
      MILTEATMN PEEIKAEYT SSLQDLTFN SKPLINVLT 
      MLAEENLAH APYIVQAIE EHLEKVQTN VKLPILYLI 
      DCIAKNIGQ TYMQLFSQN IVKTFCSVF KVVDEETRA 
      EMFKLRQTW NNVFPQMKL YAIDVQIRM LDPAWPVTA 
      QPTSNSIHF NPKFLKTTK TEIATPIVP ETTTTPLDM 
      ETFTMQQKL IQKQKELIE LQQKKLKLE LLQTQVKLQ 
      KQLENNPNA VVGNNVPPR TQTGTGKTY TMIGNQAIN 
      PEDSTIGLI PRAAAHLLE ELEQINSKI EYTVRISFI 
      EIYNEEVHD LLNSSSIPL KIFEDPDNK GSVCIKGVK 
      EATVLNLQE VYDWLNIGL VERQTASTN MNRHSSRSH 
      SIFTISVLT RQLTVEGDE LITIGKLYL VDLAGSENV 
      GRSGSTELR AREAGNINK SLLTLGKVI KALAQKTQH 
      VPYRDSKLT RILQDSLGG KTKTCMIAT ISAAANVWE 
      ETVSTLDYA QVARNVSNC PQVNENRKQ ANILKDLKD 
      EISRLRKEL AIARSGEGF YTSRENHER LCADVERLQ 
      TEVNEEKRK LEQVTKKNS ELELAISEK DEKLRNVSQ 
      YCDDLKREL REKDLQEKR NKSVIDYFE TREKEMRQA 
      AEQLRKVCE VSTKHETLF CQKYENQRA ISLKNATAA 
      KNAYNLFGD SLRALQNAT EDHIQRQNV SLSEVQANV 
      GCLKENAWK MCKRLTEYS AKMQDDSQG DFYKLDESN 
      LSRDFEQRL ESMFTSQFF AIQKKAVQD LEQYNEEKM 
      HEVETMTTR CINSMKQSL NDYMLCVND IMKNQSSLI 
      ASVKGATNS DEVNILLKP EVAKQLMPE VAKQLIPAA 
      TRDTKSKSG LGTRAQQTT PKITPVNSA LVAAATKPT 
      RDPRLLRQQ TEKTTNGTN NSSSTSVSL GSKSSILDS 
      NNKVVTNTK SVRDNRIES RLVTNKDNA PPPKLDKSK 
      TFLKSRQSE QKPNKPPRS KNSADNDSK PFSGKSSIN 
      LLDSPVKIK SDLKSPTKS PNRHKKKDH STDKKGFKD 
      AKRDRDNKG HKNDSLQTT FKGVKAKNR NYIRRNRSP 
      SYSPEPNQD TDLRVSGPP EKQARLHPE TVEEKSKLV 
      AQTGMDVDL RQLNDVTKK RASSEGLEN SNKKSKTEV 
      FDALFGNED TDLRTVLPV AQPERPPTP PPPIISLTD 
      KEDSPKKSS DFDAVRAKL ANATGRDKI MAKSFRKKR 
      TQDEDLRVP PVSSRISDT IKKIIISSD DAQCIKTGT 
      MSKEKENQL MNKIIAHIE QQKLEEAQL RDSEETVGN 
      LQPVSDEEL EFSEETDEK FGDKDDRVN FHKYSRDSR 
      ESRRGGSYW RGGKRRHWE NPHMGPHPR SAIRPWLHQ 
      MHAWRPIGP NFNRQVHTP EHDFSSQEP DGKKSPETA 
      VEVTVDSVN QDDIKTINI DAHPRDIRY YDETAIVFM 
      NWDDPREIS FQNGSRRVT FDDKDTYLL SFNEGYKEL 
      CINDTIHKV RLGAPSREI FIDGKPYEC YFGGPGITI 
      DLNGKMTVV KLDGPPPQV KIGTVKRTD LVAGKVNLI 
      IDAKILVPV FLDAKVQKF VIDGETHTL KFVDALKTV 
      LINDVPFKV EFGGLPKPI TVHDKKHFI RFSVLPRGV 
      KPGLVCIKD MEGVQAPET DESQDTALA VDINEPALP 
      VFSRKKKVE DEQDRNSNS PHFFQSFMQ QNNLDVLTS 
      VMTSSMTPS ATSGGYQVE NVGPNTETH SMDAIPYVK 
      PAPVLPSSL NINELFQKL VATGIVATQ EPVAEPQPP 
      PPVKTVRRS TMENLRPVT FNRPETLKM RQSLLYEAL 
      YSGMQCSSC GMRFPPEQS IHYSQHLDW HFRQNRKGK 
      KNIRVAASR RWYYSLSDW KNYEEIEDL EEREKNYFD 
      QQQLQAEGA GDDVDEEVE VPSVPADPD VQDAHCEVC 
      QDRFDHFFN EEKEEWHLR NAVRVEDKT YHPVCYEDY 
      QASLLEPAE ECKMEEPEP EETKIEEAN IPGLEIILD 
      DDDEEEEEE GKFKDEEVI IEESDESKP SNELKEENE 
      NEEDGEDDD VIIHEVVPE KIEVDDDKE YDTLPPSDV 
      PIKVEQIDD GFMDVEEGL IKIKNGGQI KIKSEPIDP 
      DEEMLEVPR EESSVPFTE PVQPSFTSS IDGNTEPVS 
      SVPTTTANI GSKIKINIS KPLPVIAPK EPKDISCDS 
      VPSLEPVID PSQPLPPGE EPVQLNIKP ALRGIELKK 
      LPPVQKGSE LTGMCSIM
    CDS sequence
    • >TC008263 RA
      atgattttg acggaggcc acgatgaat cctgaggag 
      atcaaggcg gagtacacc tccagcctc caagatctc 
      actttcaac agtaagccc ctgatcaat gtgttgacg 
      atgttagcg gaagaaaat ctagcacac gctccatac 
      atcgtccag gcaatcgaa gagcatctg gaaaaggtt 
      cagaccaat gtcaaactt cctattctg tatctcatc 
      gactgtatc gccaagaat attggacaa acttatatg 
      caactcttc agccaaaac atcgtcaag acattctgc 
      agcgttttt aaagtggtg gacgaagaa acgcgtgct 
      gaaatgttt aaacttcgt caaacgtgg aataacgtc 
      tttcctcaa atgaaattg tacgcgatc gacgtccag 
      attcggatg ttagatcca gcatggcca gttacagcc 
      caacctaca tccaacagt attcatttc aatcccaaa 
      tttctaaaa accaccaaa accgaaata gcaacacct 
      atcgtccct gaaacaaca accactcct ctggatatg 
      gaaaccttc acgatgcaa caaaaacta atccagaaa 
      caaaaagaa ctcatcgaa cttcagcag aaaaaatta 
      aaattagaa ctgttacaa acacaagtt aaactccaa 
      aagcaattg gaaaataat ccgaatgct gttgttggc 
      aataatgta ccaccaaga actcagacc gggacggga 
      aaaacttac acaatgatc gggaatcaa gcaataaat 
      cctgaagat tcaacaatt ggtttaata cccagggcc 
      gcagctcat ttattggaa gaattggaa caaatcaat 
      tcgaaaatt gagtacact gttcgcatt tcctttatc 
      gagatttac aacgaggaa gtgcacgat cttttaaac 
      agttcatca atcccgttg aaaatattt gaagatccg 
      gataacaaa ggatcagtt tgtatcaaa ggtgttaaa 
      gaggcgact gttcttaat ttgcaagaa gtttacgat 
      tggcttaat attggtttg gttgaacga caaacagca 
      tcgaccaat atgaatcgt cacagctct cggtcccat 
      tcaatattt acaatatcg gttctaact cgccaatta 
      acagtagaa ggagatgaa ttaattacg atcggtaaa 
      ctctatttg gttgattta gccggtagt gagaatgta 
      gggcggtcg ggttctacg gaactgaga gcacgagaa 
      gccggcaac ataaacaaa tcacttctg acattggga 
      aaagtgatc aaagccttg gctcagaaa actcagcac 
      gtaccgtat cgagactca aagttgacc agaattctg 
      caagatagt ttgggaggt aaaacaaag acgtgtatg 
      attgcgacg atttcggca gccgcgaac gtgtgggaa 
      gaaaccgta agcacgttg gattacgct caagtggca 
      cgaaatgtt tccaattgt ccccaagtg aacgaaaac 
      agaaaacaa gccaatatt ctcaaggat ctaaaggac 
      gagattagc agacttcga aaagaactg gctatagcc 
      aggtcgggc gaaggattc tacacaagc cgagaaaat 
      cacgaaaga ttatgtgca gacgtggag agattacaa 
      accgaagtc aatgaggaa aaacgaaag cttgaacaa 
      gtcacgaag aaaaacagc gagctggaa ttggcgatt 
      agtgaaaaa gacgagaaa ttacgaaat gttagtcaa 
      tattgcgac gatttgaaa cgtgagttg agagaaaaa 
      gacttgcaa gagaagcga aacaaaagc gtgattgat 
      tattttgaa acgcgcgaa aaagaaatg agacaagcg 
      gctgaacag ttacggaaa gtatgtgaa gtttcgacg 
      aaacacgaa actttgttc tgccagaag tatgaaaat 
      cagcgcgcg attagtttg aaaaacgcc acagcggcc 
      aaaaatgca tataattta tttggtgac tcgttgagg 
      gctttgcag aacgcgacg gaagatcat attcagcgt 
      cagaatgtc tcgctgagt gaggtacaa gcgaatgtc 
      gggtgtttg aaggaaaac gcctggaaa atgtgcaag 
      cgtttgact gagtattcg gcaaaaatg caagatgat 
      tcacaaggc gatttttat aaattggat gaaagtaat 
      ctttcgcgt gattttgaa caacgtttg gagtccatg 
      tttacgtcc cagtttttt gcaattcaa aagaaagcg 
      gttcaagac ttggaacag tataatgaa gaaaaaatg 
      cacgaggtg gagacgatg acaacgcgt tgtataaat 
      tcaatgaag cagtcgctg aacgattat atgctttgt 
      gttaacgat attatgaaa aaccagtcg tcgttgatt 
      gcaagtgtt aaaggcgca acgaatagt gatgaagtg 
      aatatttta ttaaaaccc gaggtagca aagcaacta 
      atgccagaa gtggcgaag cagttaatt cctgccgca 
      acacgtgat accaaaagc aaatcaggt ttgggtacg 
      agagcccag caaaccacg cctaaaata acaccagta 
      aatagcgct ttagtggcg gctgcaaca aaaccaacg 
      cgtgatcct cgtttattg cgccaacaa acggagaag 
      actaccaac ggcaccaac aacagtagt agtactagt 
      gtttcatta ggatctaag agttcaata ttagattct 
      aataataaa gttgttaca aatacaaag agcgttcga 
      gacaaccgt atcgaatct cgtttagtt actaataaa 
      gacaatgct cctccaccc aaactagac aaatccaaa 
      accttccta aagtccaga caatccgaa caaaaacca 
      aacaaacca ccacgtagc aaaaacagt gcagacaac 
      gattcgaaa cctttctcg ggtaaaagt tcgatcaat 
      ctgttagat tcacctgtt aagattaaa agtgacttg 
      aaatcgccg accaaatca cctaatagg cacaaaaag 
      aaagatcat agtacggat aaaaagggt ttcaaagac 
      gctaagcgt gacagggac aataagggt cataaaaat 
      gactcgttg caaactact ttcaaaggt gttaaggcc 
      aaaaatcgg aattatatt agaagaaat cgctcgcct 
      tcgtacagt cccgaaccc aatcaagac accgacttg 
      agagtatca ggacctcca gaaaagcaa gcacgacta 
      catccagaa actgtcgaa gaaaagagc aaattagtc 
      gcacaaacg ggaatggac gtggattta agacagttg 
      aacgacgtg acaaagaaa agagcttcg agtgaaggt 
      ctggagaac tcaaacaag aaatcgaaa acggaagta 
      tttgatgct ttgttcgga aacgaagat acggatctt 
      cgcactgtt ctaccggtg gcccagcca gaacgaccc 
      ccaactcct ccaccgcca atcatctct ctcaccgac 
      aaagaagat tcgccaaag aaatcttca gactttgat 
      gccgttcga gcgaaacta gccaatgcg accggacgc 
      gataaaata atggccaag tccttccga aaaaagcga 
      actcaggac gaagatctc cgagtacct ccagtgtca 
      agcagaatc agcgacacc atcaagaaa atcatcatt 
      tcttcggac gatgcgcag tgcatcaaa actggaaca 
      atgtcgaag gagaaagaa aatcaactt atgaacaag 
      atcatcgca cacattgag caacagaag ctggaagaa 
      gcacaattg agggatagt gaagagacg gttggtaac 
      cttcaacca gtcagcgac gaagagctc gaatttagc 
      gaagagact gacgaaaaa ttcggggat aaggacgat 
      agagtcaat ttccataaa tattccagg gattcccgg 
      gagtctaga aggggtggg tcgtattgg agaggcggc 
      aagagacgc cattgggaa aatcctcac atgggacca 
      cacccacga tctgcaata aggccttgg ttgcatcaa 
      atgcacgcc tggaggcca attgggccc aatttcaac 
      cgccaagtg cacacccct gaacacgat ttttcaagc 
      caagagcct gatggaaag aaatcaccg gaaactgca 
      gtggaagta actgtggat tcagtaaat caggacgat 
      attaagacc atcaatatc gatgcgcat ccgcgtgat 
      ataagatac tacgacgag actgcgatc gtatttatg 
      aattgggac gatccacga gagatttcg ttccaaaac 
      ggcagtcgt cgtgtgacg ttcgacgat aaagatact 
      tatttattg tccttcaat gaaggctat aaagaactg 
      tgtattaac gacacgata cataaagtg aggttgggg 
      gccccaagt cgtgagatc ttcatcgat gggaaaccc 
      tacgagtgt tacttcggg ggtcccgga ataacgata 
      gatttaaac gggaaaatg accgtagtt aaattagat 
      gggccccca cctcaggtg aaaatcggc accgtgaaa 
      agaaccgat ctggttgcc ggcaaagta aatctgata 
      atcgatgcg aaaatctta gttcccgtt tttctggat 
      gccaaagtg caaaagttt gttatcgat ggtgaaaca 
      catacatta aaatttgta gatgctttg aaaactgtt 
      ttaataaat gatgtacca tttaaagtt gaatttggt 
      ggcctccct aaacctata actgtccat gataagaaa 
      cactttatc agattttca gtgttgccg aggggtgtt 
      aaaccaggt cttgtgtgt attaaagac atggaaggg 
      gtgcaagcg cctgagact gacgaaagt caggacaca 
      gcgcttgcc gttgacatt aacgagcct gctctgccc 
      gtcttctcc cgcaagaaa aaggtggaa gatgagcaa 
      gacaggaac tcgaactcg ccacatttc ttccagagt 
      ttcatgcag caaaataat ttggacgtg cttacaagt 
      gtgatgact tcctcgatg acaccaagt gccaccagt 
      ggaggttac caagttgaa aatgtgggg ccgaacact 
      gagactcat tccatggac gccatcccc tacgtgaaa 
      ccagcaccg gtcttgccg tcctcgctc aacatcaac 
      gaactgttc caaaagttg gtagcgacg ggaatcgtc 
      gcgacccaa gaaccggta gccgaacca caaccacca 
      ccacctgtt aaaactgtg agacgcagc acgatggaa 
      aacctcaga cctgttacc ttcaacaga cctgaaacg 
      ctgaaaatg cgccaaagt cttctgtac gaagcactt 
      tactcaggg atgcagtgc agcagttgc ggcatgcgt 
      ttcccgcca gagcagagt atacactac agccaacac 
      ttggactgg catttccga caaaaccgg aaagggaaa 
      aagaacatc agagttgcg gcgagtcgc agatggtac 
      tattcactc tccgattgg aagaattac gaagaaatt 
      gaagatttg gaggaacgg gagaagaac tatttcgac 
      caacagcag ctgcaagct gagggtgct ggtgatgat 
      gttgatgaa gaagttgaa gtgccaagt gttccagcg 
      gatcctgac gtacaagac gcccattgt gaagtgtgc 
      caagacaga ttcgaccat ttcttcaac gaagagaag 
      gaagaatgg catttgaga aatgcggtt agagttgaa 
      gataagact tatcatcct gtttgctac gaagattat 
      caagcatcg ttgctagaa ccggctgaa gaatgtaaa 
      atggaagag ccggaaccg gaagaaact aaaattgaa 
      gaagcgaat atccctgga cttgaaatc attttggac 
      gatgacgac gaggaagaa gaagaagaa gggaagttt 
      aaggacgaa gaagttata attgaagaa agcgacgag 
      tcgaagcca tcgaacgaa ttgaaagaa gaaaatgaa 
      aatgaagaa gatggcgaa gatgatgat gtaatcatc 
      catgaagtt gtacctgag aaaattgaa gtggacgat 
      gacaaagaa tatgacact ttaccaccg tccgatgtg 
      cccatcaag gtggagcaa attgatgat ggttttatg 
      gacgttgaa gaaggccta attaaaatt aaaaacggg 
      ggacaaatt aaaattaaa agtgaaccg attgatccc 
      gatgaagaa atgttagag gtgccccga gaagagtca 
      agcgtgcca tttacagag ccggttcag ccctcgttt 
      acaagttcg attgatggt aataccgag cccgtttcg 
      agtgttccg acgacgaca gcaaatatc ggaagtaaa 
      attaaaata aatataagt aaaccttta cctgtgatc 
      gccccaaaa gagccgaaa gatatttcg tgtgatagc 
      gtgcctagt ttagaaccg gttatagat ccatcacag 
      cctctaccg ccgggggaa gaaccagtc caattaaat 
      atcaaacca gctctgaga ggcatagaa ctgaagaaa 
      ttaccgcca gtgcaaaaa ggaagtgaa ctgacggga 
      atgtgtagt ataatgtga
    mRNA sequence
    • >TC008263 RA
      aagtgaaaa aacgcaacg ttctgcgtc cgccatatt 
      tgtttgaga gtgtgtgtt gtgtgttaa ttggacttt 
      tctgcagtt taaactaat caaatttgg ttttatgca 
      acatttttt agatgaata cgcttaagg gattaatgt 
      tttactctt caaggacac tcacatgat tttgacgga 
      ggccacgat gaatcctga ggagatcaa ggcggagta 
      cacctccag cctccaaga tctcacttt caacagtaa 
      gcccctgat caatgtgtt gacgatgtt agcggaaga 
      aaatctagc acacgctcc atacatcgt ccaggcaat 
      cgaagagca tctggaaaa ggttcagac caatgtcaa 
      acttcctat tctgtatct catcgactg tatcgccaa 
      gaatattgg acaaactta tatgcaact cttcagcca 
      aaacatcgt caagacatt ctgcagcgt ttttaaagt 
      ggtggacga agaaacgcg tgctgaaat gtttaaact 
      tcgtcaaac gtggaataa cgtctttcc tcaaatgaa 
      attgtacgc gatcgacgt ccagattcg gatgttaga 
      tccagcatg gccagttac agcccaacc tacatccaa 
      cagtattca tttcaatcc caaatttct aaaaaccac 
      caaaaccga aatagcaac acctatcgt ccctgaaac 
      aacaaccac tcctctgga tatggaaac cttcacgat 
      gcaacaaaa actaatcca gaaacaaaa agaactcat 
      cgaacttca gcagaaaaa attaaaatt agaactgtt 
      acaaacaca agttaaact ccaaaagca attggaaaa 
      taatccgaa tgctgttgt tggcaataa tgtaccacc 
      aagaactca gaccgggac gggaaaaac ttacacaat 
      gatcgggaa tcaagcaat aaatcctga agattcaac 
      aattggttt aatacccag ggccgcagc tcatttatt 
      ggaagaatt ggaacaaat caattcgaa aattgagta 
      cactgttcg catttcctt tatcgagat ttacaacga 
      ggaagtgca cgatctttt aaacagttc atcaatccc 
      gttgaaaat atttgaaga tccggataa caaaggatc 
      agtttgtat caaaggtgt taaagaggc gactgttct 
      taatttgca agaagttta cgattggct taatattgg 
      tttggttga acgacaaac agcatcgac caatatgaa 
      tcgtcacag ctctcggtc ccattcaat atttacaat 
      atcggttct aactcgcca attaacagt agaaggaga 
      tgaattaat tacgatcgg taaactcta tttggttga 
      tttagccgg tagtgagaa tgtagggcg gtcgggttc 
      tacggaact gagagcacg agaagccgg caacataaa 
      caaatcact tctgacatt gggaaaagt gatcaaagc 
      cttggctca gaaaactca gcacgtacc gtatcgaga 
      ctcaaagtt gaccagaat tctgcaaga tagtttggg 
      aggtaaaac aaagacgtg tatgattgc gacgatttc 
      ggcagccgc gaacgtgtg ggaagaaac cgtaagcac 
      gttggatta cgctcaagt ggcacgaaa tgtttccaa 
      ttgtcccca agtgaacga aaacagaaa acaagccaa 
      tattctcaa ggatctaaa ggacgagat tagcagact 
      tcgaaaaga actggctat agccaggtc gggcgaagg 
      attctacac aagccgaga aaatcacga aagattatg 
      tgcagacgt ggagagatt acaaaccga agtcaatga 
      ggaaaaacg aaagcttga acaagtcac gaagaaaaa 
      cagcgagct ggaattggc gattagtga aaaagacga 
      gaaattacg aaatgttag tcaatattg cgacgattt 
      gaaacgtga gttgagaga aaaagactt gcaagagaa 
      gcgaaacaa aagcgtgat tgattattt tgaaacgcg 
      cgaaaaaga aatgagaca agcggctga acagttacg 
      gaaagtatg tgaagtttc gacgaaaca cgaaacttt 
      gttctgcca gaagtatga aaatcagcg cgcgattag 
      tttgaaaaa cgccacagc ggccaaaaa tgcatataa 
      tttatttgg tgactcgtt gagggcttt gcagaacgc 
      gacggaaga tcatattca gcgtcagaa tgtctcgct 
      gagtgaggt acaagcgaa tgtcgggtg tttgaagga 
      aaacgcctg gaaaatgtg caagcgttt gactgagta 
      ttcggcaaa aatgcaaga tgattcaca aggcgattt 
      ttataaatt ggatgaaag taatctttc gcgtgattt 
      tgaacaacg tttggagtc catgtttac gtcccagtt 
      ttttgcaat tcaaaagaa agcggttca agacttgga 
      acagtataa tgaagaaaa aatgcacga ggtggagac 
      gatgacaac gcgttgtat aaattcaat gaagcagtc 
      gctgaacga ttatatgct ttgtgttaa cgatattat 
      gaaaaacca gtcgtcgtt gattgcaag tgttaaagg 
      cgcaacgaa tagtgatga agtgaatat tttattaaa 
      acccgaggt agcaaagca actaatgcc agaagtggc 
      gaagcagtt aattcctgc cgcaacacg tgataccaa 
      aagcaaatc aggtttggg tacgagagc ccagcaaac 
      cacgcctaa aataacacc agtaaatag cgctttagt 
      ggcggctgc aacaaaacc aacgcgtga tcctcgttt 
      attgcgcca acaaacgga gaagactac caacggcac 
      caacaacag tagtagtac tagtgtttc attaggatc 
      taagagttc aatattaga ttctaataa taaagttgt 
      tacaaatac aaagagcgt tcgagacaa ccgtatcga 
      atctcgttt agttactaa taaagacaa tgctcctcc 
      acccaaact agacaaatc caaaacctt cctaaagtc 
      cagacaatc cgaacaaaa accaaacaa accaccacg 
      tagcaaaaa cagtgcaga caacgattc gaaaccttt 
      ctcgggtaa aagttcgat caatctgtt agattcacc 
      tgttaagat taaaagtga cttgaaatc gccgaccaa 
      atcacctaa taggcacaa aaagaaaga tcatagtac 
      ggataaaaa gggtttcaa agacgctaa gcgtgacag 
      ggacaataa gggtcataa aaatgactc gttgcaaac 
      tactttcaa aggtgttaa ggccaaaaa tcggaatta 
      tattagaag aaatcgctc gccttcgta cagtcccga 
      acccaatca agacaccga cttgagagt atcaggacc 
      tccagaaaa gcaagcacg actacatcc agaaactgt 
      cgaagaaaa gagcaaatt agtcgcaca aacgggaat 
      ggacgtgga tttaagaca gttgaacga cgtgacaaa 
      gaaaagagc ttcgagtga aggtctgga gaactcaaa 
      caagaaatc gaaaacgga agtatttga tgctttgtt 
      cggaaacga agatacgga tcttcgcac tgttctacc 
      ggtggccca gccagaacg acccccaac tcctccacc 
      gccaatcat ctctctcac cgacaaaga agattcgcc 
      aaagaaatc ttcagactt tgatgccgt tcgagcgaa 
      actagccaa tgcgaccgg acgcgataa aataatggc 
      caagtcctt ccgaaaaaa gcgaactca ggacgaaga 
      tctccgagt acctccagt gtcaagcag aatcagcga 
      caccatcaa gaaaatcat catttcttc ggacgatgc 
      gcagtgcat caaaactgg aacaatgtc gaaggagaa 
      agaaaatca acttatgaa caagatcat cgcacacat 
      tgagcaaca gaagctgga agaagcaca attgaggga 
      tagtgaaga gacggttgg taaccttca accagtcag 
      cgacgaaga gctcgaatt tagcgaaga gactgacga 
      aaaattcgg ggataagga cgatagagt caatttcca 
      taaatattc cagggattc ccgggagtc tagaagggg 
      tgggtcgta ttggagagg cggcaagag acgccattg 
      ggaaaatcc tcacatggg accacaccc acgatctgc 
      aataaggcc ttggttgca tcaaatgca cgcctggag 
      gccaattgg gcccaattt caaccgcca agtgcacac 
      ccctgaaca cgatttttc aagccaaga gcctgatgg 
      aaagaaatc accggaaac tgcagtgga agtaactgt 
      ggattcagt aaatcagga cgatattaa gaccatcaa 
      tatcgatgc gcatccgcg tgatataag atactacga 
      cgagactgc gatcgtatt tatgaattg ggacgatcc 
      acgagagat ttcgttcca aaacggcag tcgtcgtgt 
      gacgttcga cgataaaga tacttattt attgtcctt 
      caatgaagg ctataaaga actgtgtat taacgacac 
      gatacataa agtgaggtt gggggcccc aagtcgtga 
      gatcttcat cgatgggaa accctacga gtgttactt 
      cgggggtcc cggaataac gatagattt aaacgggaa 
      aatgaccgt agttaaatt agatgggcc cccacctca 
      ggtgaaaat cggcaccgt gaaaagaac cgatctggt 
      tgccggcaa agtaaatct gataatcga tgcgaaaat 
      cttagttcc cgtttttct ggatgccaa agtgcaaaa 
      gtttgttat cgatggtga aacacatac attaaaatt 
      tgtagatgc tttgaaaac tgttttaat aaatgatgt 
      accatttaa agttgaatt tggtggcct ccctaaacc 
      tataactgt ccatgataa gaaacactt tatcagatt 
      ttcagtgtt gccgagggg tgttaaacc aggtcttgt 
      gtgtattaa agacatgga aggggtgca agcgcctga 
      gactgacga aagtcagga cacagcgct tgccgttga 
      cattaacga gcctgctct gcccgtctt ctcccgcaa 
      gaaaaaggt ggaagatga gcaagacag gaactcgaa 
      ctcgccaca tttcttcca gagtttcat gcagcaaaa 
      taatttgga cgtgcttac aagtgtgat gacttcctc 
      gatgacacc aagtgccac cagtggagg ttaccaagt 
      tgaaaatgt ggggccgaa cactgagac tcattccat 
      ggacgccat cccctacgt gaaaccagc accggtctt 
      gccgtcctc gctcaacat caacgaact gttccaaaa 
      gttggtagc gacgggaat cgtcgcgac ccaagaacc 
      ggtagccga accacaacc accaccacc tgttaaaac 
      tgtgagacg cagcacgat ggaaaacct cagacctgt 
      taccttcaa cagacctga aacgctgaa aatgcgcca 
      aagtcttct gtacgaagc actttactc agggatgca 
      gtgcagcag ttgcggcat gcgtttccc gccagagca 
      gagtataca ctacagcca acacttgga ctggcattt 
      ccgacaaaa ccggaaagg gaaaaagaa catcagagt 
      tgcggcgag tcgcagatg gtactattc actctccga 
      ttggaagaa ttacgaaga aattgaaga tttggagga 
      acgggagaa gaactattt cgaccaaca gcagctgca 
      agctgaggg tgctggtga tgatgttga tgaagaagt 
      tgaagtgcc aagtgttcc agcggatcc tgacgtaca 
      agacgccca ttgtgaagt gtgccaaga cagattcga 
      ccatttctt caacgaaga gaaggaaga atggcattt 
      gagaaatgc ggttagagt tgaagataa gacttatca 
      tcctgtttg ctacgaaga ttatcaagc atcgttgct 
      agaaccggc tgaagaatg taaaatgga agagccgga 
      accggaaga aactaaaat tgaagaagc gaatatccc 
      tggacttga aatcatttt ggacgatga cgacgagga 
      agaagaaga agaagggaa gtttaagga cgaagaagt 
      tataattga agaaagcga cgagtcgaa gccatcgaa 
      cgaattgaa agaagaaaa tgaaaatga agaagatgg 
      cgaagatga tgatgtaat catccatga agttgtacc 
      tgagaaaat tgaagtgga cgatgacaa agaatatga 
      cactttacc accgtccga tgtgcccat caaggtgga 
      gcaaattga tgatggttt tatggacgt tgaagaagg 
      cctaattaa aattaaaaa cgggggaca aattaaaat 
      taaaagtga accgattga tcccgatga agaaatgtt 
      agaggtgcc ccgagaaga gtcaagcgt gccatttac 
      agagccggt tcagccctc gtttacaag ttcgattga 
      tggtaatac cgagcccgt ttcgagtgt tccgacgac 
      gacagcaaa tatcggaag taaaattaa aataaatat 
      aagtaaacc tttacctgt gatcgcccc aaaagagcc 
      gaaagatat ttcgtgtga tagcgtgcc tagtttaga 
      accggttat agatccatc acagcctct accgccggg 
      ggaagaacc agtccaatt aaatatcaa accagctct 
      gagaggcat agaactgaa gaaattacc gccagtgca 
      aaaaggaag tgaactgac gggaatgtg tagtataat 
      gtgattaat ttaaatagt gctgagtga gtgtgtgta 
      catattgca gactaaaag ggggactgt ttatataaa 
      attcgtttc gttttatca tctgcacta aaatggatt 
      ttttacgat ttattttca ctctgtacc tattatgcg 
      tatcatgat ttagtttga cattttatg tctctgttg 
      ttgtcaatt gttggaaat aaatttatt ttttaaaa
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