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Gene information
General information
TC008243 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 7162123..7167696
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008243
    iBeetle screen iB_02769
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008243 RA
      MVLLRKTDM EDTMPQTVP SAGFTPPCD YTNFAENFD 
      LSLLPGGEQ VDYTQSPPP EKPQPKIYT TFYPNSPEA 
      DFRPDFQTL LVPDAEKTP AVFLEVPPI LSPNSYRQS 
      SEANFYPSS PELSLYTPS PNSLYSQSP VDDFFQYVK 
      KEAYPPSPY SYRVKEENF LSPCGFSSP TSPATSYSS 
      LSSPEQINT QQSENTDVL ELLENTVLD KQIKQESTV 
      DFGNLLSTF QDSESLRRL LEDDFKEAQ PQTVPSKPK 
      DHQLLREVL RDTSFQKKY NIKPIDFGF IGDIKMEEP 
      DENAQRDLM REKIEPVLS LAIEQMRKD VDTTCAALG 
      ISPDPTQWS AADVLSWLQ WTSRQFGLT EPVPDHWDM 
      NGPSLAALS EEDFTRRAP QGGMILHAQ LEIWKAAYS 
      DEDLSMKWQ PETTSSNAS SGDMSDEDD DEASSSQTD 
      PSRPTTSRS GGSHIHLWQ FLKELLASP QTYGSCIRW 
      LDRTKGIFK IEDSVKVAR LWGKRKNRP AMNYDKLSR 
      SIRQYYKKG IMKKTERSQ RLVYQFCHP YNL
    CDS sequence
    • >TC008243 RA
      atggttctt ttaaggaaa acggacatg gaagacacg 
      atgccacaa actgttcct tcagccggg ttcacccca 
      ccgtgtgac tacacaaac ttcgctgaa aacttcgac 
      ttgtccctc ctccccggt ggcgagcaa gttgactac 
      acacagtcg ccaccaccc gaaaaaccc caaccgaaa 
      atctacacc accttctac cccaactcg cctgaagcc 
      gacttccgg cccgatttc cagacgttg ctggtgcca 
      gacgccgaa aaaacccca gctgtgttt ctggaagta 
      ccgccgatc ctctcaccc aactcctat cgccagtcg 
      agcgaagcc aacttctac cccagttcg ccggagttg 
      tccctgtac accccgtcc ccgaactcg ctctactcg 
      caatcgcca gtcgatgac tttttccag tacgtcaaa 
      aaggaagcc tatccgccc tccccgtac agttacaga 
      gtcaaagag gagaatttt ctctcgcct tgcggtttt 
      tcatcgccg acaagtcct gccacaagc tactcgagt 
      ttgtcgtca ccagaacaa ataaacaca cagcagtcg 
      gagaacacc gacgtctta gagttactg gaaaacacc 
      gtcttggat aaacaaatc aagcaagag agcacggtc 
      gatttcggc aatctcttg agtactttc caggattcg 
      gaaagcttg agacgcttg ctggaagac gacttcaag 
      gaggcgcag ccccagacg gtgccctcc aagccgaaa 
      gaccaccaa ctgttgcga gaggttttg cgagatacc 
      agcttccag aagaagtat aacattaag ccgatcgat 
      ttcgggttc attggcgac attaagatg gaagaaccg 
      gacgagaac gcccagagg gacctcatg agggagaaa 
      atcgagcct gtgttgagc ctcgccatc gagcagatg 
      aggaaggac gtggacacg acgtgtgca gcgcttggc 
      atatccccc gatcccacg caatggagc gctgcagat 
      gtgctgtcc tggctgcag tggacctcc cgacagttc 
      ggtttaacc gaaccggtt ccagaccac tgggacatg 
      aacggcccg agtttggcc gccttgtcc gaggaagat 
      tttacaaga cgtgctcct cagggagga atgatcctt 
      catgcccag cttgaaata tggaaagct gcatactct 
      gacgaggat ctaagcatg aaatggcaa cctgagacc 
      acctccagt aatgcttca agtggagac atgtcggat 
      gaagacgac gacgaagcg tcttcatcg cagacagac 
      ccgagtcgg cccacgacc tccaggtcg gggggttcc 
      cacatccac ttgtggcag ttcctgaag gaactgttg 
      gcttcgccg cagacttac ggctcctgc atccggtgg 
      ctggaccgc acgaaggga atcttcaag atcgaggac 
      tccgtcaaa gtggccagg ctgtggggg aagaggaag 
      aatcggccg gcgatgaac tacgataag ttgagtagg 
      tcgataagg cagtattac aaaaaggga atcatgaag 
      aagacggag cggtcgcag aggctcgtc taccagttt 
      tgccacccg tacaacttg tag
    mRNA sequence
    • >TC008243 RA
      gctgtaacc aaatttcgc cgaaacgta tttttttct 
      tcaaaatcg ggagcttgt cgatggata taaacttta 
      tttaaacat ttgtgggat ttgtgacaa acagtatgg 
      ttcttttaa ggaaaacgg acatggaag acacgatgc 
      cacaaactg ttccttcag ccgggttca ccccaccgt 
      gtgactaca caaacttcg ctgaaaact tcgacttgt 
      ccctcctcc ccggtggcg agcaagttg actacacac 
      agtcgccac cacccgaaa aaccccaac cgaaaatct 
      acaccacct tctacccca actcgcctg aagccgact 
      tccggcccg atttccaga cgttgctgg tgccagacg 
      ccgaaaaaa ccccagctg tgtttctgg aagtaccgc 
      cgatcctct cacccaact cctatcgcc agtcgagcg 
      aagccaact tctacccca gttcgccgg agttgtccc 
      tgtacaccc cgtccccga actcgctct actcgcaat 
      cgccagtcg atgactttt tccagtacg tcaaaaagg 
      aagcctatc cgccctccc cgtacagtt acagagtca 
      aagaggaga attttctct cgccttgcg gtttttcat 
      cgccgacaa gtcctgcca caagctact cgagtttgt 
      cgtcaccag aacaaataa acacacagc agtcggaga 
      acaccgacg tcttagagt tactggaaa acaccgtct 
      tggataaac aaatcaagc aagagagca cggtcgatt 
      tcggcaatc tcttgagta ctttccagg attcggaaa 
      gcttgagac gcttgctgg aagacgact tcaaggagg 
      cgcagcccc agacggtgc cctccaagc cgaaagacc 
      accaactgt tgcgagagg ttttgcgag ataccagct 
      tccagaaga agtataaca ttaagccga tcgatttcg 
      ggttcattg gcgacatta agatggaag aaccggacg 
      agaacgccc agagggacc tcatgaggg agaaaatcg 
      agcctgtgt tgagcctcg ccatcgagc agatgagga 
      aggacgtgg acacgacgt gtgcagcgc ttggcatat 
      cccccgatc ccacgcaat ggagcgctg cagatgtgc 
      tgtcctggc tgcagtgga cctcccgac agttcggtt 
      taaccgaac cggttccag accactggg acatgaacg 
      gcccgagtt tggccgcct tgtccgagg aagatttta 
      caagacgtg ctcctcagg gaggaatga tccttcatg 
      cccagcttg aaatatgga aagctgcat actctgacg 
      aggatctaa gcatgaaat ggcaacctg agaccacct 
      ccagtaatg cttcaagtg gagacatgt cggatgaag 
      acgacgacg aagcgtctt catcgcaga cagacccga 
      gtcggccca cgacctcca ggtcggggg gttcccaca 
      tccacttgt ggcagttcc tgaaggaac tgttggctt 
      cgccgcaga cttacggct cctgcatcc ggtggctgg 
      accgcacga agggaatct tcaagatcg aggactccg 
      tcaaagtgg ccaggctgt gggggaaga ggaagaatc 
      ggccggcga tgaactacg ataagttga gtaggtcga 
      taaggcagt attacaaaa agggaatca tgaagaaga 
      cggagcggt cgcagaggc tcgtctacc agttttgcc 
      acccgtaca acttgtagt tgtgcactg tttatttat 
      ttttatttt tgttagggg ttacgtcgg gctggtgtg 
      gggtttcgt tggcgtttt gaagttatc aaaatgcaa 
      atgaatttt tggtcattt cgtagggta ttactgcga 
      aatttttaa atttgtaaa tatttatta ttcatgaac 
      ggaaggtaa tttccaaat attttgata taatttaag 
      taaaatatt tgccgataa gtagaaatt ttcgcggag 
      agtttactg gtgttcagt ccttgtggc ggtttggtg 
      ctcgagccc aggcttttg agctcagtt gccactttg 
      ggttagcac tggtcttcc aaaatttcg aaattcggt 
      gattttggt cccatatat ctatgatat attaagtaa 
      gtaagttct atgaacacg attatattt cttaaggtt 
      aataataaa atactcatg gacatatcc agattcgta 
      cacagtgta cagtaagtg ctatagctg tggctgaaa 
      taaatatac tatgtgtta atcctaatt tattgttat 
      ttgtttaac aaatgtctt ctattgtat gttattttt 
      agatgttga tagctttaa gcagttatt tcttgtgca 
      aatttattt ttaataaaa cttattacc aaaa