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Gene information
General information
TC032845 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 6737210..6745692
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032845
    iBeetle screen iB_02768
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032845 RA
      MVSFSLMTF MAVINACTC ANILMITMG GTKSHKIPF 
      WELARGLIP RGHNITFIS AFPSDFLLP GLEEITPTS 
      FVFYVKNFT NWDLVGAKI RGEEPVHPF DMVRYAYEA 
      CDVLLSDPE TKDLLYSQQ SFDLLVLDG AYPECALGF 
      VNHYNAPFI YINTVGFYT GSLSLAGNP APYSVTPFL 
      ARPFTDAMS LLQRTVNTV WHLLANSLH SFMVRNMIQ 
      PIVRKHFGP DVPLVYDIS RNVSFILQN AHATVTYPR 
      PYLPNVAEI ACIHCKRAK ALPDDLEEF IQGSGSAGF 
      IYFSMGSSV KAANMPEYL RRMLMRVFR QLPQRVLWK 
      YEADEEMPD LPANVKLGR WLPQQDILG HPKIRAFVT 
      HGGLLSMFE TVYHGVPVV SLPVFCDHD SNAAKAETD 
      GYALKLDLS KITAESLVW AIRKVIHDP KYREEVKKR 
      QFLLRDQKE TPLERAVYW TEYVLRHKG ATHLQSPAK 
      NMGVVEYYL IDVALLASC CTFIVWYLM KLTLKIIVM 
      VLSGNLSFN LFRKKIKIE
    CDS sequence
    • >TC032845 RA
      atggtcagc ttctcgtta atgaccttt atggctgtg 
      ataaacgcg tgtacctgc gccaatatt ttaatgata 
      acgatgggt ggcacaaag tctcacaaa attcccttc 
      tgggagttg gctcgagga ctcattccc agaggccac 
      aacatcaca ttcatcagc gcttttccc tctgacttc 
      ctcctgccc ggcctcgag gaaatcacc ccgaccagt 
      ttcgtcttc tacgtgaag aatttcacc aactgggat 
      ttggtcggg gctaagatc aggggcgaa gagcccgtt 
      caccccttc gacatggtc cgatacgct tacgaggct 
      tgtgatgta cttctttca gatccggaa acgaaagac 
      ttgctctat tcccagcag agtttcgac cttttggtc 
      ctggacggc gcctatccg gagtgcgct ttaggcttc 
      gtcaaccac tacaacgcc cctttcatc tacattaac 
      acagttggg ttctacacc ggatcgctg tctctggcg 
      ggcaatcct gcgccttat tcggtgaca ccgtttctt 
      gcaaggccg tttaccgac gcaatgagt ttactgcaa 
      aggactgtg aacacggtc tggcacctg ctggccaac 
      tccctgcac tcgttcatg gtcagaaac atgatacag 
      cccatcgtc agaaagcac ttcgggcct gatgtcccc 
      ctggtctat gacatttcc aggaatgtc agctttatt 
      ctgcaaaat gcacacgcc acggtcact tatcctaga 
      ccttatttg cctaatgtg gcggaaatt gcgtgcata 
      cattgcaaa agggctaag gctctgcct gatgacttg 
      gaggagttt atccaaggc agtgggagc gctggtttc 
      atctacttc agcatgggc tcgtctgta aaagcggcc 
      aacatgccg gagtacctc cggaggatg ctgatgcgc 
      gtcttccgg cagcttccc cagcgggtc ctctggaaa 
      tacgaggcc gacgaggaa atgcccgac ctgcccgcc 
      aacgtcaaa cttggccga tggctacca caacaagac 
      attttaggt catccaaaa attcgagca ttcgtcacc 
      cacggaggt ctattgagc atgttcgag acggtgtac 
      catggagta ccagtcgtt tcactgccg gttttttgc 
      gaccacgat tctaacgct gccaaagcc gaaaccgac 
      ggctatgct ttgaaactc gacttgagc aaaatcaca 
      gctgagtcg ctagtctgg gccatcagg aaagtcata 
      cacgacccc aaatatcgg gaggaagtg aaaaagaga 
      cagtttttg ttgcgggat cagaaggaa accccgctc 
      gaaagagcc gtctactgg acagagtac gtcttgagg 
      cacaaaggg gccacgcac ctgcagtcc cctgccaaa 
      aatatgggg gttgtagag tattattta attgatgtg 
      gcattgtta gcgtcctgt tgtacattt attgtttgg 
      tatttaatg aaattgacc ctaaaaatc attgttatg 
      gtcttgtca ggtaattta agctttaat ttatttagg 
      aaaaaaatt aaaatagaa tag
    mRNA sequence
    • >TC032845 RA
      aagcagtac cattaccat ttctcctcc actttcaac 
      ttcgttccg ctataacca actagtatt cagtttact 
      ttctgactg aaggcacta caattattt aatggttct 
      gttcggcac agcaacaac atgagggat cgcagtttg 
      cggtacaaa aatcagtga ttgcttgta ctcaaaagt 
      gcccagtgg tgtttagta tgtgttttg actagtagt 
      ttcagaaag gattatcat tttattccc aaaaataac 
      caacacaaa aatggtcag cttctcgtt aatgacctt 
      tatggctgt gataaacgc gtgtacctg cgccaatat 
      tttaatgat aacgatggg tggcacaaa gtctcacaa 
      aattccctt ctgggagtt ggctcgagg actcattcc 
      cagaggcca caacatcac attcatcag cgcttttcc 
      ctctgactt cctcctgcc cggcctcga ggaaatcac 
      cccgaccag tttcgtctt ctacgtgaa gaatttcac 
      caactggga tttggtcgg ggctaagat caggggcga 
      agagcccgt tcacccctt cgacatggt ccgatacgc 
      ttacgaggc ttgtgatgt acttctttc agatccgga 
      aacgaaaga cttgctcta ttcccagca gagtttcga 
      ccttttggt cctggacgg cgcctatcc ggagtgcgc 
      tttaggctt cgtcaacca ctacaacgc ccctttcat 
      ctacattaa cacagttgg gttctacac cggatcgct 
      gtctctggc gggcaatcc tgcgcctta ttcggtgac 
      accgtttct tgcaaggcc gtttaccga cgcaatgag 
      tttactgca aaggactgt gaacacggt ctggcacct 
      gctggccaa ctccctgca ctcgttcat ggtcagaaa 
      catgataca gcccatcgt cagaaagca cttcgggcc 
      tgatgtccc cctggtcta tgacatttc caggaatgt 
      cagctttat tctgcaaaa tgcacacgc cacggtcac 
      ttatcctag accttattt gcctaatgt ggcggaaat 
      tgcgtgcat acattgcaa aagggctaa ggctctgcc 
      tgatgactt ggaggagtt tatccaagg cagtgggag 
      cgctggttt catctactt cagcatggg ctcgtctgt 
      aaaagcggc caacatgcc ggagtacct ccggaggat 
      gctgatgcg cgtcttccg gcagcttcc ccagcgggt 
      cctctggaa atacgaggc cgacgagga aatgcccga 
      cctgcccgc caacgtcaa acttggccg atggctacc 
      acaacaaga cattttagg tcatccaaa aattcgagc 
      attcgtcac ccacggagg tctattgag catgttcga 
      gacggtgta ccatggagt accagtcgt ttcactgcc 
      ggttttttg cgaccacga ttctaacgc tgccaaagc 
      cgaaaccga cggctatgc tttgaaact cgacttgag 
      caaaatcac agctgagtc gctagtctg ggccatcag 
      gaaagtcat acacgaccc caaatatcg ggaggaagt 
      gaaaaagag acagttttt gttgcggga tcagaagga 
      aaccccgct cgaaagagc cgtctactg gacagagta 
      cgtcttgag gcacaaagg ggccacgca cctgcagtc 
      ccctgccaa aaatatggg ggttgtaga gtattattt 
      aattgatgt ggcattgtt agcgtcctg ttgtacatt 
      tattgtttg gtatttaat gaaattgac cctaaaaat 
      cattgttat ggtcttgtc aggtaattt aagctttaa 
      tttatttag gaaaaaaat taaaataga atagacatt 
      aaacgagtg taaatatg