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Gene information
General information
TC007263 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6701088..6705704
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007263
    iBeetle screen iB_02767

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva potentially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007263 RA
      MLCHNVFTF LLLACVLRA SLCQDNNVC PSYRDYITM 
KNNSNCWYN FAADLKVPE IIRRSGYPF IEYKVQTKD 
GYILSVFRI PSVQQKAPV FMLHGIQST SGIFVGMGK 
HSLAFLLAD AGYDVWLGN YRGTEYSEG HTHLNITQR 
DYWNYGVDE IALIDVPTM LNLVRYYTW KRGKIIYIG 
HSLGTSAAM MYACEYQEH AKETVKLFI FMAPAYKLN 
NMRSPYRVF FPLMRTALS LSNNLNIVQ VFSRGYARR 
LTRPTCLAA PSLMLLCLS LVNLFLGPF TQISPETVP 
VLFNQLPGG TSLKTLTYL SEAVRGQFR KFDYGGRNL 
FMYGNSTPP SYNISRVEV PVFIFYASH DWATSKPDA 
INLYRSLPP ASRFGIYEI SNLRFNHFD FLFGKEAKT 
LVHDKILQV IENFPNKTP LR
    CDS sequence
    • >TC007263 RA
      atgttgtgc cataacgtg ttcaccttt cttctgcta 
gcgtgtgtg ctcagagca tcgttatgt caagacaat 
aacgtgtgt ccgagttat cgggattat ataaccatg 
aagaacaac tccaactgt tggtacaac ttcgccgcc 
gatctcaaa gtgccggag attatacga agatctggc 
tatcccttc atagaatac aaagtccag accaaagac 
ggctacatc ctctcggtc ttcaggata ccgagtgtg 
cagcaaaaa gcgcccgtg ttcatgcta cacggcatc 
cagagcact tcgggcatt tttgtcggc atgggaaag 
cattccctt gcctttctc ctcgcagac gcaggctat 
gatgtttgg ttgggcaat tatcgcggc actgaatac 
tccgaaggc catacgcac ttgaacatt acccaaagg 
gactactgg aactacgga gtcgacgaa attgccctg 
atcgacgtc ccgactatg ctgaactta gtcaggtat 
tacacgtgg aaaagaggg aagattatc tacatcggg 
cactcgcta ggaacgtcg gctgcgatg atgtacgct 
tgtgaatat caagaacat gccaaagaa actgttaaa 
ttattcata tttatggct cctgcatac aagctaaat 
aatatgagg tcgccgtat cgggtgttt ttcccttta 
atgaggaca gcgctgtcc ctttccaac aacctgaac 
attgttcag gtgttttct cgaggatat gcgagacga 
ctcactcga cccacgtgt ctcgcagcc ccgtcttta 
atgcttctt tgcttgagc ttagtcaat ctgtttctc 
gggcccttc acgcagatc tctccggag acagtccct 
gttcttttc aaccaatta ccgggcggt acttcgtta 
aaaactctt acctatcta tcggaggct gtaagaggc 
cagtttaga aaattcgat tacggtggg agaaatttg 
ttcatgtat ggtaattcc acaccaccg tcgtataat 
atttccaga gtggaagta ccagttttt attttttat 
gcctcgcat gattgggcg acttcgaaa cctgatgca 
atcaacttg taccgatcg ttaccaccg gcttccagg 
ttcggtatt tatgaaata tcgaacctc aggttcaac 
cacttcgat tttctcttc gggaaagaa gcaaagact 
ctggtccac gacaaaatc ctccaagtg attgagaat 
tttcctaat aaaacgcct ctccggtga
    mRNA sequence
    • >TC007263 RA
      gcagttaaa cggatagcc tcctggaaa aatgttgtg 
ccataacgt gttcacctt tcttctgct agcgtgtgt 
gctcagagc atcgttatg tcaagacaa taacgtgtg 
tccgagtta tcgggatta tataaccat gaagaacaa 
ctccaactg ttggtacaa cttcgccgc cgatctcaa 
agtgccgga gattatacg aagatctgg ctatccctt 
catagaata caaagtcca gaccaaaga cggctacat 
cctctcggt cttcaggat accgagtgt gcagcaaaa 
agcgcccgt gttcatgct acacggcat ccagagcac 
ttcgggcat ttttgtcgg catgggaaa gcattccct 
tgcctttct cctcgcaga cgcaggcta tgatgtttg 
gttgggcaa ttatcgcgg cactgaata ctccgaagg 
ccatacgca cttgaacat tacccaaag ggactactg 
gaactacgg agtcgacga aattgccct gatcgacgt 
cccgactat gctgaactt agtcaggta ttacacgtg 
gaaaagagg gaagattat ctacatcgg gcactcgct 
aggaacgtc ggctgcgat gatgtacgc ttgtgaata 
tcaagaaca tgccaaaga aactgttaa attattcat 
atttatggc tcctgcata caagctaaa taatatgag 
gtcgccgta tcgggtgtt tttcccttt aatgaggac 
agcgctgtc cctttccaa caacctgaa cattgttca 
ggtgttttc tcgaggata tgcgagacg actcactcg 
acccacgtg tctcgcagc cccgtcttt aatgcttct 
ttgcttgag cttagtcaa tctgtttct cgggccctt 
cacgcagat ctctccgga gacagtccc tgttctttt 
caaccaatt accgggcgg tacttcgtt aaaaactct 
tacctatct atcggaggc tgtaagagg ccagtttag 
aaaattcga ttacggtgg gagaaattt gttcatgta 
tggtaattc cacaccacc gtcgtataa tatttccag 
agtggaagt accagtttt tatttttta tgcctcgca 
tgattgggc gacttcgaa acctgatgc aatcaactt 
gtaccgatc gttaccacc ggcttccag gttcggtat 
ttatgaaat atcgaacct caggttcaa ccacttcga 
ttttctctt cgggaaaga agcaaagac tctggtcca 
cgacaaaat cctccaagt gattgagaa ttttcctaa 
taaaacgcc tctccggtg atgaactgt gttactgcc 
ttattgtta ttgtagatt tctatgtaa ataaatatt 
ttattcgct a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY