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Gene information
General information
TC008190 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6352046..6381180
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC008190
    iBeetle screen iB_02765

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC008190 RA
      MPSSNPLPP KENALFKRI LRCYEQKQY KNGLKFAKQ 
      ILSNPKFTE HGETLAMKG LTLNCLGRK EEAYEYVRR 
      GLRNDLRSH VCWHVYGLL QRADKKYDE AIKCYRNAL 
      KWEKDNMQI LRDLSLLQI QMRDLEGYR DTRYQLLML 
      RPTQRASWI GYAMSFHLL EDYKNALNI LETFLDQQQ 
      KGNNYDYEH SELLLYQNL VIQESGDLK EALKHLESS 
      QDQIVDKLT LKENLGELN LKLKNFDKA AKYYEELIQ 
      RNPENTMYY NKLIEAKKL TKPEDVVNF YLECSEKYP 
      RAMPPRRLP LNYAVGEQF KTLVDKYMR RSLSKGVPP 
      LFVDLRSLY TDKSKVEII ENLMLQYVD ALKKVGKYS 
      EAELNNGPK EPASALLWV YYYLAQHHD YLNQTEKAV 
      SYIDAAIEH TPTLIELFV TKGRIYKHA GDPQEAYKW 
      LDEAQALDT ADRYINSKC AKYMLRANR VKEAEDTCA 
      KFTREGVSA MENLNEMQC MWFETECAL AYQRLGKYG 
      EALKKCHET DRHFSEIIE DQFDFHTYC MRKMTLRSY 
      VGLLRLEDV LRGHPFYFK AAKCAIEVY LHLFDEPLK 
      DENAEQELN TENLAPSEL KKLRNKQRK ARRKAEQES 
      AQAREAQVK RDHHHKSRQ QGDVEADAP QLDELIPDK 
      LARVEDPLE QAIKFLQPL QKLAKNRIE THLMAFEVY 
      YRKKKVLLM LQSLKRAHQ IAPQNPKLH SCLIRFYEV 
      IEQNKNSWD PAVEQVVTK EVQILFKGK DAKQLNKEF 
      LEAHSHSLE AVLECCRMM FYLDNKTQS TALSLVTNL 
      DNKYEDVTL QNCVNVAEA LRRGDFGSC DAQLEDYIK 
      KCHNRFPFA ADFKPASAS PSESNHVSQ DQDNCNSN
    CDS sequence
    • >TC008190 RA
      atgccctca agcaatccg ctaccaccg aaagaaaat 
      gccttattt aaaagaatt ttgagatgt tacgaacag 
      aagcaatac aaaaatggg ctgaaattt gcaaagcag 
      atcctgtca aaccccaaa ttcacagag catggagag 
      accttggcc atgaaaggc ctgacgctg aactgtctc 
      gggcgcaaa gaggaggcc tacgagtac gtgaggaga 
      ggtttgagg aacgacttg cgctcgcac gtttgttgg 
      catgtttac ggactttta caaagggct gacaaaaag 
      tacgacgaa gccatcaag tgttatcgc aatgcgctc 
      aagtgggaa aaggacaat atgcaaatt cttagggac 
      ctgtctctg ctacagata caaatgcgc gatttggag 
      gggtacaga gacacacgc taccagctg cttatgctc 
      aggccaacg cagagagct tcctggatc ggttatgct 
      atgtcgttt catttgtta gaagattac aagaacgca 
      ttgaacata ttagagaca tttttggac caacagcag 
      aaagggaat aattacgat tacgagcac agcgaattg 
      ttactttat caaaatctg gtaatccaa gagtctggc 
      gatttaaaa gaggccttg aaacattta gaatcctcc 
      caagatcag atcgttgac aagctgacg cttaaagaa 
      aacctaggt gaactaaat ctaaaattg aaaaatttc 
      gataaagcc gcaaaatat tacgaggaa ctaatccaa 
      agaaatccg gaaaatacc atgtactat aataaatta 
      atcgaagcg aaaaaactg accaaaccc gaagacgtt 
      gttaatttt tacctagag tgttccgaa aaatatccg 
      cgagcgatg ccgccacga agactgcca ttaaattac 
      gcggtcggt gaacaattt aaaactctc gtcgataaa 
      tacatgagg aggtcgctg tcgaaaggc gtacctcca 
      cttttcgtc gatttgcgg tcgttatat acagataag 
      agtaaagtg gaaattatc gaaaattta atgttacaa 
      tacgtggat gcgctaaag aaagtgggc aaatacagt 
      gaagccgaa ctgaacaat ggacccaaa gaaccagcg 
      tctgcactg ctctgggtt tactattat ctagcacag 
      catcacgat tatctcaac cagacagaa aaggctgtg 
      agctacatt gatgccgcc attgaacac acaccgacc 
      ttaatcgaa ctcttcgtt accaaaggc aggatctac 
      aagcacgcc ggcgatcca caagaggcg tacaaatgg 
      ctcgacgag gcgcaagcc ctcgacacc gccgaccgt 
      tacatcaat tcgaaatgc gccaaatat atgctcagg 
      gcaaatcgg gtaaaagaa gccgaagac acctgtgcc 
      aagttcaca cgagagggt gtttcagcg atggaaaat 
      ctcaacgaa atgcaatgc atgtggttc gaaaccgaa 
      tgtgctttg gcctaccag cgcctgggc aaatacggg 
      gaggctttg aagaaatgc cacgagacg gacaggcac 
      ttttcggaa attatcgag gaccagttt gatttccac 
      acttattgt atgcggaaa atgacgctg cggtcgtac 
      gtggggctc ttgcgactt gaggatgtg ctcagagga 
      cacccgttt tatttcaaa gccgccaaa tgtgccatt 
      gaagtgtac ctgcatctg ttcgatgag cccctcaag 
      gacgagaac gccgaacag gagctcaac accgaaaat 
      ttggcacct tccgaattg aagaaattg cggaacaag 
      cagcgaaaa gctcgcagg aaggcggaa caagagagt 
      gctcaagca agagaagcg caagtgaaa cgtgaccac 
      caccataaa tcacgccag caaggggat gtagaagcg 
      gatgcacct cagttagac gaactaatc cccgataag 
      ctggcacgg gtcgaagat ccgctcgaa caagccatc 
      aagttcctc caacccctt caaaaatta gcaaaaaat 
      cgaatagaa acccattta atggcgttt gaggtttat 
      tatcggaaa aaaaaagtt ctgttgatg ttgcagtcc 
      ttgaaaagg gctcatcaa atcgcaccg cagaatcca 
      aagctacac agttgcctg atcagattt tacgaagtg 
      atcgaacaa aataaaaac tcgtgggat ccggccgtt 
      gaacaagtg gtgaccaaa gaagtgcaa attttgttt 
      aaaggaaaa gacgccaaa cagctcaat aaagaattc 
      ctagaggcg cactcgcat agtttagaa gccgtactc 
      gaatgctgt cgaatgatg ttctatctc gacaacaag 
      acacaaagc actgctctc agtctggtc accaatcta 
      gacaataaa tacgaggat gtcacccta caaaactgc 
      gtgaatgtg gcggaggct ttgcgacga ggcgacttc 
      ggaagttgc gacgctcaa ttagaggat tacataaag 
      aaatgccat aaccgtttt ccgttcgcc gccgatttt 
      aaaccggcg tcggcgtcc ccaagcgag agcaatcac 
      gtgtcccaa gaccaagac aactgcaac agcaactga
    mRNA sequence
    • >TC008190 RA
      atgccctca agcaatccg ctaccaccg aaagaaaat 
      gccttattt aaaagaatt ttgagatgt tacgaacag 
      aagcaatac aaaaatggg ctgaaattt gcaaagcag 
      atcctgtca aaccccaaa ttcacagag catggagag 
      accttggcc atgaaaggc ctgacgctg aactgtctc 
      gggcgcaaa gaggaggcc tacgagtac gtgaggaga 
      ggtttgagg aacgacttg cgctcgcac gtttgttgg 
      catgtttac ggactttta caaagggct gacaaaaag 
      tacgacgaa gccatcaag tgttatcgc aatgcgctc 
      aagtgggaa aaggacaat atgcaaatt cttagggac 
      ctgtctctg ctacagata caaatgcgc gatttggag 
      gggtacaga gacacacgc taccagctg cttatgctc 
      aggccaacg cagagagct tcctggatc ggttatgct 
      atgtcgttt catttgtta gaagattac aagaacgca 
      ttgaacata ttagagaca tttttggac caacagcag 
      aaagggaat aattacgat tacgagcac agcgaattg 
      ttactttat caaaatctg gtaatccaa gagtctggc 
      gatttaaaa gaggccttg aaacattta gaatcctcc 
      caagatcag atcgttgac aagctgacg cttaaagaa 
      aacctaggt gaactaaat ctaaaattg aaaaatttc 
      gataaagcc gcaaaatat tacgaggaa ctaatccaa 
      agaaatccg gaaaatacc atgtactat aataaatta 
      atcgaagcg aaaaaactg accaaaccc gaagacgtt 
      gttaatttt tacctagag tgttccgaa aaatatccg 
      cgagcgatg ccgccacga agactgcca ttaaattac 
      gcggtcggt gaacaattt aaaactctc gtcgataaa 
      tacatgagg aggtcgctg tcgaaaggc gtacctcca 
      cttttcgtc gatttgcgg tcgttatat acagataag 
      agtaaagtg gaaattatc gaaaattta atgttacaa 
      tacgtggat gcgctaaag aaagtgggc aaatacagt 
      gaagccgaa ctgaacaat ggacccaaa gaaccagcg 
      tctgcactg ctctgggtt tactattat ctagcacag 
      catcacgat tatctcaac cagacagaa aaggctgtg 
      agctacatt gatgccgcc attgaacac acaccgacc 
      ttaatcgaa ctcttcgtt accaaaggc aggatctac 
      aagcacgcc ggcgatcca caagaggcg tacaaatgg 
      ctcgacgag gcgcaagcc ctcgacacc gccgaccgt 
      tacatcaat tcgaaatgc gccaaatat atgctcagg 
      gcaaatcgg gtaaaagaa gccgaagac acctgtgcc 
      aagttcaca cgagagggt gtttcagcg atggaaaat 
      ctcaacgaa atgcaatgc atgtggttc gaaaccgaa 
      tgtgctttg gcctaccag cgcctgggc aaatacggg 
      gaggctttg aagaaatgc cacgagacg gacaggcac 
      ttttcggaa attatcgag gaccagttt gatttccac 
      acttattgt atgcggaaa atgacgctg cggtcgtac 
      gtggggctc ttgcgactt gaggatgtg ctcagagga 
      cacccgttt tatttcaaa gccgccaaa tgtgccatt 
      gaagtgtac ctgcatctg ttcgatgag cccctcaag 
      gacgagaac gccgaacag gagctcaac accgaaaat 
      ttggcacct tccgaattg aagaaattg cggaacaag 
      cagcgaaaa gctcgcagg aaggcggaa caagagagt 
      gctcaagca agagaagcg caagtgaaa cgtgaccac 
      caccataaa tcacgccag caaggggat gtagaagcg 
      gatgcacct cagttagac gaactaatc cccgataag 
      ctggcacgg gtcgaagat ccgctcgaa caagccatc 
      aagttcctc caacccctt caaaaatta gcaaaaaat 
      cgaatagaa acccattta atggcgttt gaggtttat 
      tatcggaaa aaaaaagtt ctgttgatg ttgcagtcc 
      ttgaaaagg gctcatcaa atcgcaccg cagaatcca 
      aagctacac agttgcctg atcagattt tacgaagtg 
      atcgaacaa aataaaaac tcgtgggat ccggccgtt 
      gaacaagtg gtgaccaaa gaagtgcaa attttgttt 
      aaaggaaaa gacgccaaa cagctcaat aaagaattc 
      ctagaggcg cactcgcat agtttagaa gccgtactc 
      gaatgctgt cgaatgatg ttctatctc gacaacaag 
      acacaaagc actgctctc agtctggtc accaatcta 
      gacaataaa tacgaggat gtcacccta caaaactgc 
      gtgaatgtg gcggaggct ttgcgacga ggcgacttc 
      ggaagttgc gacgctcaa ttagaggat tacataaag 
      aaatgccat aaccgtttt ccgttcgcc gccgatttt 
      aaaccggcg tcggcgtcc ccaagcgag agcaatcac 
      gtgtcccaa gaccaagac aactgcaac agcaactga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY