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Gene information
General information
TC007357 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 5762652..5773036
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007357
    iBeetle screen iB_02764

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007357 RA
      MDEGTLNDL LECSVCLDR LDTSSKVLP CQHTFCRKC 
      LQEIVHKHK ELRCPECRI LVNSKVDDL PPNVLLMRI 
      LEGMKNNAV PKKQQRAPK SIQAPAVHH TPVHQQQKI 
      NNNKTSPQL VPHQPYAKA LYDYEPKEV GDLSFKRGD 
      VILLRKRID AHWYQGECG GKQGLFPLS YVQIITPLP 
      SHIPQCKAL YDFQTDKHE EEGCLTFKE GDIINVIRR 
      VDENWAEGK LDGRIGIFP LTFVELNSL ARSLMKLST 
      NAQPGPSRV APPTPTTED STPLIPTDH SRTIVPQQT 
      QNQQLFVQQ TPVHQGLAT SDSSSTVSS GSSSTTTPN 
      VSSSNTSSN SSTAPSSPA SPPPRTQPP LARTLVHAP 
      QRSPNTAPS HQHNKEKRH SFTSLTPAT YNKNTHRHS 
      AEIVAAETA SPSSHSHKK PEDEIKLPA PYVALYPYK 
      PQKADELEL RKGGIYMVT ERCQDGWYK GTSNRTQKC 
      GVFPGNYVA HMSKASLSS SDAKVAVSF TRSGKSVSS 
      PRQTYNTLP PELPPRSVS PATATNTIS SSWHGQQDN 
      AAVPLGRSS SAVMSSVNT THLSLGSAT VAKPADKCK 
      DRKEKGTVG LMRRLTSIK RSKSPPPSS YSMDNPVFE 
      DSVSVATAH PVHVRSGSC PSQLLQIQP VEHHRLFGS 
      TSQRLKHKE RPSVLTHNL RAENPAGSS SSSPDIHHH 
      RKSNSLDAG NRTKTGVQT VHELFRCEI PYPPNSEYE 
      LELQKDDLI YVHKKREDG WYKGTLQRT GRTGLFPAS 
      FVKPA
    CDS sequence
    • >TC007357 RA
      atggacgag ggaacactc aacgacctc ctggaatgc 
      tccgtgtgc ctggaccgc ctggacacc tccagcaag 
      gtgctgccc tgccagcac accttctgc cggaagtgc 
      ctccaggag atcgtccac aagcacaaa gagctgcgc 
      tgccccgag tgccgcatc ctcgtcaat tcgaaggtg 
      gacgacttg cccccgaac gtgctcctg atgcggatc 
      ctcgaggga atgaagaac aatgccgtt ccgaagaag 
      caacaaagg gctccgaag agcattcaa gcacctgct 
      gtgcatcac acgccggtg caccagcag cagaagatc 
      aacaataac aagaccagt ccgcagctg gtgccccat 
      cagccctac gccaaggct ctctacgat tacgaacca 
      aaggaagtg ggcgatttg agttttaaa cgtggcgat 
      gtaatactt cttcgaaaa cgaatcgat gctcactgg 
      tatcaagga gaatgtggc ggaaaacaa ggtctattt 
      cccctaagt tacgtccag atcataact cctttacct 
      agtcacatt cctcaatgt aaagccctc tacgatttc 
      caaaccgac aaacacgag gaagaagga tgtctcaca 
      ttcaaagaa ggcgacata atcaatgtt ataagaaga 
      gtagacgaa aactgggcc gaaggaaaa cttgacgga 
      cgtatcgga atttttcct ctcactttc gtggaattg 
      aatagttta gcacgttcg ctcatgaag ttatcaaca 
      aacgcacaa cctggacct tcaagggtc gctcctccg 
      acgcctaca accgaagac agcactccg ctgatacca 
      acggatcat tcgaggaca atagtgcca caacaaacg 
      caaaatcaa caacttttc gtccaacaa acaccagtc 
      caccagggt ctagcaacg tccgattcg agctccacc 
      gtcagctct ggcagctcc tcgaccact acaccgaat 
      gtgtcttcc agcaacacc tcttcgaat tcgagcacg 
      gcaccgtct tcgccggcc tctccgccg cctaggacg 
      cagccacca ctcgcccgg accctagtc catgcgccc 
      cagcggtcc ccgaacacc gctccaagc caccaacac 
      aacaaggag aaacgccac agtttcaca agcttgacc 
      cccgccaca tacaacaaa aatactcat cggcattct 
      gccgaaatt gtggctgcg gaaacagcg tcgccgagt 
      agccacagc cacaaaaag cccgaggac gagatcaaa 
      ctcccagct ccgtacgtg gccttgtac ccctacaag 
      ccccaaaag gccgacgag ctggagctg cgcaagggc 
      ggcatctac atggtgacc gaacggtgc caggatggc 
      tggtacaag ggcacctcg aaccgtacg caaaaatgc 
      ggcgttttt cccgggaat tacgtggcg cacatgtcg 
      aaggcgtcg ctgagttcg tccgatgcg aaagttgcc 
      gtaagtttt acaaggagt ggtaaaagt gtgtcttcg 
      ccacgccaa acctataac actttacct cctgagttg 
      ccgccacgt tctgttagt cctgctacc gcaacaaat 
      actatttcg tccagctgg catggacag caggataat 
      gccgccgtg cctctagga cgtagtagt agcgctgtt 
      atgtccagt gtgaacacg actcatctg agtttgggt 
      agtgcgact gttgcaaag cctgcagat aagtgcaag 
      gaccgtaaa gaaaagggt actgttggt ttaatgcgt 
      agacttact agcattaaa cgatccaag tcaccacct 
      ccatcctca tattctatg gataatcct gtctttgaa 
      gactcagtt agcgttgct acagctcat cctgtacat 
      gtcaggtct ggatcatgc ccgagtcaa ctgctgcaa 
      attcagcct gttgaacac caccgcctt ttcggctcg 
      acttcccaa cgtttaaaa cacaaagaa cgtcccagt 
      gttcttacg cacaatcta agagcagaa aatccggcc 
      ggaagctca agctccagt ccggacatc catcaccat 
      cgaaagtcg aattctctg gacgcggga aatcgcacc 
      aaaacaggg gtgcaaacg gtccatgaa ctgttccgg 
      tgcgaaatt ccgtatccg ccgaacagc gagtacgaa 
      ttggagttg caaaaggac gatctgatt tatgtccat 
      aagaagagg gaggacggg tggtataaa gggacgctg 
      caaaggacg ggaagaacg ggattattt cctgctagt 
      ttcgtcaaa ccggcttga
    mRNA sequence
    • >TC007357 RA
      tatgctaaa taatgtggt cttgcaacg gaagtcaaa 
      gatgtgtgc taccacgcc accgtggtt aaagttctt 
      gaacattgg ctcaagtgc gctttggct ctggttggt 
      tttggtact tattcccag ttcttcgac gcatgcccc 
      cagaaattc atcccaatc ttcggaaaa atcattggg 
      gggattaaa ctcgatcgc cctcgcgcc gcgtaggcc 
      aatgcgcaa ggaagaatg catgttgtt gcttgttat 
      gagttcgta gtcggtcac cgcgcccaa cgatgactg 
      agccactgt gttaaaccg gaagtggcg gaagccagc 
      actagtgtt acaaatgtc gatcactcg caatcgctg 
      tgttatttc tagtgctct ccactatct gataatctc 
      ccacattgt ctcggatta tcgcccatc agttatcag 
      tttggcgtg aggcatgga cgagggaac actcaacga 
      cctcctgga atgctccgt gtgcctgga ccgcctgga 
      cacctccag caaggtgct gccctgcca gcacacctt 
      ctgccggaa gtgcctcca ggagatcgt ccacaagca 
      caaagagct gcgctgccc cgagtgccg catcctcgt 
      caattcgaa ggtggacga cttgccccc gaacgtgct 
      cctgatgcg gatcctcga gggaatgaa gaacaatgc 
      cgttccgaa gaagcaaca aagggctcc gaagagcat 
      tcaagcacc tgctgtgca tcacacgcc ggtgcacca 
      gcagcagaa gatcaacaa taacaagac cagtccgca 
      gctggtgcc ccatcagcc ctacgccaa ggctctcta 
      cgattacga accaaagga agtgggcga tttgagttt 
      taaacgtgg cgatgtaat acttcttcg aaaacgaat 
      cgatgctca ctggtatca aggagaatg tggcggaaa 
      acaaggtct atttcccct aagttacgt ccagatcat 
      aactccttt acctagtca cattcctca atgtaaagc 
      cctctacga tttccaaac cgacaaaca cgaggaaga 
      aggatgtct cacattcaa agaaggcga cataatcaa 
      tgttataag aagagtaga cgaaaactg ggccgaagg 
      aaaacttga cggacgtat cggaatttt tcctctcac 
      tttcgtgga attgaatag tttagcacg ttcgctcat 
      gaagttatc aacaaacgc acaacctgg accttcaag 
      ggtcgctcc tccgacgcc tacaaccga agacagcac 
      tccgctgat accaacgga tcattcgag gacaatagt 
      gccacaaca aacgcaaaa tcaacaact tttcgtcca 
      acaaacacc agtccacca gggtctagc aacgtccga 
      ttcgagctc caccgtcag ctctggcag ctcctcgac 
      cactacacc gaatgtgtc ttccagcaa cacctcttc 
      gaattcgag cacggcacc gtcttcgcc ggcctctcc 
      gccgcctag gacgcagcc accactcgc ccggaccct 
      agtccatgc gccccagcg gtccccgaa caccgctcc 
      aagccacca acacaacaa ggagaaacg ccacagttt 
      cacaagctt gacccccgc cacatacaa caaaaatac 
      tcatcggca ttctgccga aattgtggc tgcggaaac 
      agcgtcgcc gagtagcca cagccacaa aaagcccga 
      ggacgagat caaactccc agctccgta cgtggcctt 
      gtaccccta caagcccca aaaggccga cgagctgga 
      gctgcgcaa gggcggcat ctacatggt gaccgaacg 
      gtgccagga tggctggta caagggcac ctcgaaccg 
      tacgcaaaa atgcggcgt ttttcccgg gaattacgt 
      ggcgcacat gtcgaaggc gtcgctgag ttcgtccga 
      tgcgaaagt tgccgtaag ttttacaag gagtggtaa 
      aagtgtgtc ttcgccacg ccaaaccta taacacttt 
      acctcctga gttgccgcc acgttctgt tagtcctgc 
      taccgcaac aaatactat ttcgtccag ctggcatgg 
      acagcagga taatgccgc cgtgcctct aggacgtag 
      tagtagcgc tgttatgtc cagtgtgaa cacgactca 
      tctgagttt gggtagtgc gactgttgc aaagcctgc 
      agataagtg caaggaccg taaagaaaa gggtactgt 
      tggtttaat gcgtagact tactagcat taaacgatc 
      caagtcacc acctccatc ctcatattc tatggataa 
      tcctgtctt tgaagactc agttagcgt tgctacagc 
      tcatcctgt acatgtcag gtctggatc atgcccgag 
      tcaactgct gcaaattca gcctgttga acaccaccg 
      ccttttcgg ctcgacttc ccaacgttt aaaacacaa 
      agaacgtcc cagtgttct tacgcacaa tctaagagc 
      agaaaatcc ggccggaag ctcaagctc cagtccgga 
      catccatca ccatcgaaa gtcgaattc tctggacgc 
      gggaaatcg caccaaaac aggggtgca aacggtcca 
      tgaactgtt ccggtgcga aattccgta tccgccgaa 
      cagcgagta cgaattgga gttgcaaaa ggacgatct 
      gatttatgt ccataagaa gagggagga cgggtggta 
      taaagggac gctgcaaag gacgggaag aacgggatt 
      atttcctgc tagtttcgt caaaccggc ttgaaacaa 
      aattaggtg ttgtaacta taatttagg gccaaaggt 
      tgttataac cataataac caaataagt tgtgtttaa 
      tagctcaat taatcgtgt ccatataac catgacttt 
      tactctaga ttttttcgc cataactag ttgaaattg 
      taaataaaa acaaaacca agttttaaa attgtatat 
      tggtatttt tttaggtgt agttatgaa gcgttcgat 
      catttcaaa tgtatagat tgaatagga tacatcatg 
      tgttcaaga ttatgtatt tacttgcga ttttaactt 
      taatttatt tttaaattc gttacctaa agactattt 
      attggggtg gaggatttt ttatcaaca tatcacgat 
      tcggtcgcg tttttgaat cgtctttaa atttaattt 
      catgaacaa ataatgact gattgtgaa caatgcgtt 
      gactagtat atgacatat ttttattaa tgtgcatta 
      acaaatatt gtaaattga aatgtattc tgatatatt 
      acagttaat tgcaatttt gccgctagt ttttttacc 
      cagcactat aacttagga gacgaaacg caggatttt 
      ttaaatcca aaaaaatag tacggtaga gtaaaaatg 
      agggcgcca attcgtttt aaattaatt taaatacaa 
      ccaattcac taat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY