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Gene information
General information
TC008086 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4917903..4919863
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC008086
      iBeetle screen iB_02763

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC008086 RA
        MRVFIAFFL YFALSEGRV ITFDLSNYE PQNATTQVQ 
        KVHVNLTRV TQIWVVNIP ETVGFFIVQ IHSFPDNVT 
        LSSSPKFKS KTTIKGANV GLVSFNLSD TMFYVRNKN 
        ETRALIVVT IYQTEDPVP GGCNLSFPT EYAPYQLVS 
        NTDEVVVVQ SQPPSIIDA TCDKNKLQV DMYHLYLHE 
        NNYDPSYYF GGIERMLTV DLIKKNGYK VSTVDGYFK 
        YRKLYSAYR GVGQVFAIV ATNGNVSSA YVPAVSYSC 
        DLTQPETCT GPVTPHWKL ICAIMTIFG VFVCFFGHR 
        FFRLTLFLA GFTLGLFVT YVILSLSSN SLTVEEKAT 
        TSIIIGMIY GAVWLLVWW KFGIPIFSA GLTFFMAAF 
        LMASIIFYS GISDLWIFR NNANFWLTF ASIVVTFLT 
        IWTMWTLEG HIFGCSFLG AYSCVAALN YYIGGNLQY 
        IVINSFRRI AVKNFNMAT VDPPFQILD ISQSILLVV 
        MTTYGFYYQ MKEQRGKPP FPPHRYTRI EEGVSERTP 
        LVGNSGVIY SDI
      CDS sequence
      • >TC008086 RA
        atgcgggtt tttattgcg tttttcttg tatttcgca 
        ctcagtgaa ggacgtgtc ataacattt gatttatca 
        aattatgag ccccaaaat gccacgaca caggtacaa 
        aaagtgcac gtcaacttg acccgtgtt acgcaaatt 
        tgggtcgtt aatataccc gagactgtc ggctttttt 
        attgttcaa attcattca tttcccgat aacgtaacc 
        ctatcttca tcacctaaa ttcaagtca aaaacaaca 
        ataaaaggg gccaatgtg ggcttggtt tcctttaat 
        ttaagtgat acgatgttt tacgttaga aataaaaat 
        gaaactcgg gctttaatc gttgtgaca atttatcaa 
        accgaagat cccgttccc gggggctgt aacttgtcg 
        tttccgacc gagtatgcc ccctaccaa ctcgtctca 
        aacactgac gaagttgtt gtagtccaa agtcagccc 
        ccttcaata atcgacgcc acctgtgat aaaaacaag 
        ttacaagtc gatatgtac cacttgtat ctgcacgag 
        aataactac gacccgtct tactacttt ggcggaatt 
        gagcgaatg ttgaccgtt gacttaatt aaaaaaaac 
        ggctataaa gtctcaaca gtcgatggt tatttcaaa 
        taccgcaaa ttatactcc gcctacagg ggcgtaggt 
        caagttttc gcaatcgta gcgacaaac ggtaacgta 
        agctcggcc tacgttccc gcagtttcg tacagttgc 
        gacctaacg cagcccgaa acttgcacc ggccctgtc 
        acccctcat tggaaacta atctgcgca ataatgaca 
        atttttggg gtctttgtg tgctttttt gggcacaga 
        tttttcaga ctcaccctg tttttggcc ggttttaca 
        ctgggcctg ttcgtaact tacgttatc ttgtccttg 
        agtagcaac agtttaaca gttgaagaa aaagcgacc 
        acttcgatt attataggg atgatatac ggggcggtt 
        tggttacta gtttggtgg aaatttgga atcccgatt 
        ttctcggcc ggcttgacg tttttcatg gcggccttt 
        ttaatggcc tctattatt ttttattcg ggcatttcc 
        gatttgtgg atttttcgc aacaatgcc aacttctgg 
        ctcactttc gcctcaatt gtggtcact tttctcaca 
        atttggacg atgtggact ttggagggg cacattttc 
        gggtgtagt tttctgggg gcgtattcc tgcgttgcc 
        gcgttgaat tattacatc ggcggaaat ttgcaatat 
        attgttatt aattccttc aggagaata gccgtcaag 
        aattttaat atggcaaca gtggaccct ccgtttcaa 
        attttggat atttcgcag agtattttg ttggtggtg 
        atgaccact tatggcttt tattaccag atgaaagaa 
        caaaggggg aagccgccg ttccctcca catcggtac 
        acaagaatt gaagaaggg gtgagcgag aggactccc 
        ttggtggga aatagtggg gttatttat tctgatatt 
        taa
      mRNA sequence
      • >TC008086 RA
        accggagtg tttatgttt tggttatca aaatcgatt 
        tatcgcatt ttttggagt gaaagaatg taaaatgcg 
        ggtttttat tgcgttttt cttgtattt cgcactcag 
        tgaaggacg tgtcataac atttgattt atcaaatta 
        tgagcccca aaatgccac gacacaggt acaaaaagt 
        gcacgtcaa cttgacccg tgttacgca aatttgggt 
        cgttaatat acccgagac tgtcggctt ttttattgt 
        tcaaattca ttcatttcc cgataacgt aaccctatc 
        ttcatcacc taaattcaa gtcaaaaac aacaataaa 
        aggggccaa tgtgggctt ggtttcctt taatttaag 
        tgatacgat gttttacgt tagaaataa aaatgaaac 
        tcgggcttt aatcgttgt gacaattta tcaaaccga 
        agatcccgt tcccggggg ctgtaactt gtcgtttcc 
        gaccgagta tgcccccta ccaactcgt ctcaaacac 
        tgacgaagt tgttgtagt ccaaagtca gcccccttc 
        aataatcga cgccacctg tgataaaaa caagttaca 
        agtcgatat gtaccactt gtatctgca cgagaataa 
        ctacgaccc gtcttacta ctttggcgg aattgagcg 
        aatgttgac cgttgactt aattaaaaa aaacggcta 
        taaagtctc aacagtcga tggttattt caaataccg 
        caaattata ctccgccta caggggcgt aggtcaagt 
        tttcgcaat cgtagcgac aaacggtaa cgtaagctc 
        ggcctacgt tcccgcagt ttcgtacag ttgcgacct 
        aacgcagcc cgaaacttg caccggccc tgtcacccc 
        tcattggaa actaatctg cgcaataat gacaatttt 
        tggggtctt tgtgtgctt ttttgggca cagattttt 
        cagactcac cctgttttt ggccggttt tacactggg 
        cctgttcgt aacttacgt tatcttgtc cttgagtag 
        caacagttt aacagttga agaaaaagc gaccacttc 
        gattattat agggatgat atacggggc ggtttggtt 
        actagtttg gtggaaatt tggaatccc gattttctc 
        ggccggctt gacgttttt catggcggc ctttttaat 
        ggcctctat tatttttta ttcgggcat ttccgattt 
        gtggatttt tcgcaacaa tgccaactt ctggctcac 
        tttcgcctc aattgtggt cacttttct cacaatttg 
        gacgatgtg gactttgga ggggcacat tttcgggtg 
        tagttttct gggggcgta ttcctgcgt tgccgcgtt 
        gaattatta catcggcgg aaatttgca atatattgt 
        tattaattc cttcaggag aatagccgt caagaattt 
        taatatggc aacagtgga ccctccgtt tcaaatttt 
        ggatatttc gcagagtat tttgttggt ggtgatgac 
        cacttatgg cttttatta ccagatgaa agaacaaag 
        ggggaagcc gccgttccc tccacatcg gtacacaag 
        aattgaaga aggggtgag cgagaggac tcccttggt 
        gggaaatag tggggttat ttattctga tatttaaac 
        cagtgatac tttattgag tctgcggtg ctttattaa 
        gactctctt ttatgccag actgatgta attattttt 
        taataaaag actttttac cac
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY