Status
Please wait ...
Gene information
General information
TC007420 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4755231..4760276
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007420
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02762

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007420 RA
      MCQKCQIGI LVFCLISGG IALENGLAR TPPMGWLAW 
      ERFRCNTDC KNDPENCIS ENLFRTMAD IVISEGYAA 
      AGYEYINVD DCWLEKERD RFGQLVPDR QRFPRGMKA 
      LADYVHSKG LKFGIYEDF GNYTCAGYP GVLGYLRTD 
      AETFASWDV DYVKLDGCY AHPSEMDRG YPEFGFYLN 
      QTGRAMMYS CSWPVYQIY AGMQPNFTS IIEHCNLWR 
      NFDDIQDSW ASVESIIDY YGNNQDVIV ANAGPGHWN 
      DPDMLIIGN FGLSYEQSK TQMAIWAIL AAPLLMSVD 
      LRSIRPEYK AILQNKKII AVDQDPLGI QGRRIYKHK 
      GIEIWSRPI TPLYQNYFS YAIAFLNRR TDGTPSDVA 
      VTLKELGLT SPTGYRVED LYEDVDYGV LSSQTKIKV 
      KVNPSGVVI LRADVQNDF KRRIPYYTT QRTIYNPLS 
      QVFRIRNNG F
    CDS sequence
    • >TC007420 RA
      atgtgtcaa aaatgtcaa atcggaatt ttggtgttt 
      tgcctgatt agtggcggg attgcgctc gaaaatggc 
      ctggcgcgg acgcccccc atgggctgg ctggcttgg 
      gagcgcttc cggtgcaat accgactgc aaaaacgat 
      cccgaaaac tgcatcagc gagaacttg ttccggacg 
      atggctgat atcgtgatt tcggagggc tacgcggcc 
      gcggggtac gaatatatc aatgttgat gactgctgg 
      cttgagaaa gaaagggac aggttcggg caacttgtc 
      ccggacaga cagcggttc cctcgaggg atgaaagca 
      ctagccgac tatgtgcac tccaaaggg ctcaaattc 
      gggatttac gaagacttt ggtaactac acctgtgca 
      gggtatccc ggagttctg ggttattta agaacggac 
      gctgagacg tttgcgtcc tgggacgtg gattacgtc 
      aaactggac ggttgttac gcccatccc agtgagatg 
      gaccggggc tatcccgaa ttcgggttc tacttgaac 
      caaactggg agagcaatg atgtattcc tgcagttgg 
      cccgtttac caaatttac gcaggaatg cagcccaac 
      ttcacttca atcatcgaa cattgtaat ttgtggcga 
      aatttcgac gatatccag gactcctgg gccagtgtt 
      gaaagtatt attgattat tatgggaat aatcaggat 
      gtgattgtt gctaatgcg ggaccagga cattggaac 
      gacccagac atgttgatt atcggaaat ttcggtctg 
      agttacgag cagagcaag acccagatg gccatttgg 
      gccatcctg gcagccccc ttgctcatg tcggtcgac 
      ttgcgctcg atccggccc gagtacaaa gccattttg 
      caaaacaaa aaaatcata gccgtcgat caagatccg 
      ttgggcatt caaggaagg cgtatatac aagcataaa 
      ggtatcgaa atttggtcg agaccgatc actcctctc 
      taccagaat tatttctcc tatgcgatc gcatttctg 
      aacaggaga acggacgga acgccttca gatgtagcg 
      gtcacgctg aaggaattg ggtttgacg tcaccgacg 
      ggctacaga gtcgaggat ctctacgaa gatgtggat 
      tatggagtt ttgtcttcg caaacgaaa atcaaagtg 
      aaagtcaac ccatccggt gtcgtaatt ttaagagcc 
      gatgtacaa aacgatttt aaaagaaga attccctac 
      tatacgacg caacgaacg atttataat ccattaagt 
      caagttttc agaatacga aataacgga ttttaa
    mRNA sequence
    • >TC007420 RA
      cctccatca aagtttatt accctcttt aacacctca 
      tggaatgcg aagaagtct ttgcatcat caccatctg 
      tcagcgtgc tttattggc atccggccc actatatca 
      cttcgataa tagccccat catgtctct aaaatctgt 
      actgccggt ttagccgcg ctcgcaaac ggacgtggc 
      taggcctcc acttttacc ttcaccgtc cacaaacgg 
      caatccttt gtgttccca aggttattt aaacgtcaa 
      atagtgaaa tgtgtcaaa aatgtcaaa tcggaattt 
      tggtgtttt gcctgatta gtggcggga ttgcgctcg 
      aaaatggcc tggcgcgga cgcccccca tgggctggc 
      tggcttggg agcgcttcc ggtgcaata ccgactgca 
      aaaacgatc ccgaaaact gcatcagcg agaacttgt 
      tccggacga tggctgata tcgtgattt cggagggct 
      acgcggccg cggggtacg aatatatca atgttgatg 
      actgctggc ttgagaaag aaagggaca ggttcgggc 
      aacttgtcc cggacagac agcggttcc ctcgaggga 
      tgaaagcac tagccgact atgtgcact ccaaagggc 
      tcaaattcg ggatttacg aagactttg gtaactaca 
      cctgtgcag ggtatcccg gagttctgg gttatttaa 
      gaacggacg ctgagacgt ttgcgtcct gggacgtgg 
      attacgtca aactggacg gttgttacg cccatccca 
      gtgagatgg accggggct atcccgaat tcgggttct 
      acttgaacc aaactggga gagcaatga tgtattcct 
      gcagttggc ccgtttacc aaatttacg caggaatgc 
      agcccaact tcacttcaa tcatcgaac attgtaatt 
      tgtggcgaa atttcgacg atatccagg actcctggg 
      ccagtgttg aaagtatta ttgattatt atgggaata 
      atcaggatg tgattgttg ctaatgcgg gaccaggac 
      attggaacg acccagaca tgttgatta tcggaaatt 
      tcggtctga gttacgagc agagcaaga cccagatgg 
      ccatttggg ccatcctgg cagccccct tgctcatgt 
      cggtcgact tgcgctcga tccggcccg agtacaaag 
      ccattttgc aaaacaaaa aaatcatag ccgtcgatc 
      aagatccgt tgggcattc aaggaaggc gtatataca 
      agcataaag gtatcgaaa tttggtcga gaccgatca 
      ctcctctct accagaatt atttctcct atgcgatcg 
      catttctga acaggagaa cggacggaa cgccttcag 
      atgtagcgg tcacgctga aggaattgg gtttgacgt 
      caccgacgg gctacagag tcgaggatc tctacgaag 
      atgtggatt atggagttt tgtcttcgc aaacgaaaa 
      tcaaagtga aagtcaacc catccggtg tcgtaattt 
      taagagccg atgtacaaa acgatttta aaagaagaa 
      ttccctact atacgacgc aacgaacga tttataatc 
      cattaagtc aagttttca gaatacgaa ataacggat 
      tttaatgta aatacgacg taaattata ataatttta 
      ggtacaggt tttttactt ttttaaatt ttatttaca 
      aagtacgaa ataaattgt tagtccaaa c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY