Status
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Gene information
General information
TC032783
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007538
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3189753..3191316
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032783
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02760

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032783 RA
      MIIFLKILI FALPLTTCQ LNNACKSFE SYFNIYFSP 
      DCFYIPDNY LNAPEIIKH HVGLFEHHK VTTEDGYIL 
      GLFRIPQTS PKGVILLQH GFVQDARSW LSQYNESVA 
      FWFWKAGYD VWLSNSRGT FYSQKHSNL TVNDEEFWN 
      FTFHEIGYF DLDAVIKYV KVCTKRPKV ILVASSMGF 
      ASSLVYVSS KYKEAAENV QILIGLAPV WSLKYTKSL 
      YKWALFPSI HSILKNGLN RFISWHSWP HYDGWMLWT 
      LRMLNKVFP FKKLYIYTE SVFCGWSET GFDPSFINL 
      MIDHLGAPS SLKNFEHIY QTINNEEVR MFDYGKSRN 
      LEIYGSEMN PSYLLENVS VPLLLVYGT NDYLTQAGD 
      IDALTQKLP EEAKVFGEM KIEGFSHTD FYFGRHRYE 
      MVYKKVIEF LGNLEE
    CDS sequence
    • >TC032783 RA
      atgattatt ttcctgaaa attctcatt tttgcactt 
      cctctcacc acttgccag ttaaataat gcctgcaaa 
      agcttcgaa agctatttc aatatctat ttcagtccc 
      gactgtttt tacattcct gataactac ttaaacgcc 
      cccgaaata atcaaacat catgtaggc ttattcgaa 
      caccacaaa gttaccacc gaagatggc tacattttg 
      ggcctcttt cggattcca caaactagc ccaaagggc 
      gtaattctc ttgcagcac ggttttgtg caagacgcc 
      aggtcttgg ttgtctcag tacaacgaa tcggtggct 
      ttctggttc tggaaggcc ggttacgat gtgtggctt 
      tccaacagt cggggcact ttttactcg caaaaacat 
      tcaaatctc acagttaat gatgaggag ttttggaat 
      tttacgttt cacgaaatt ggctatttt gatttggat 
      gctgttatc aagtatgtg aaagtgtgc actaaacgt 
      cccaaggtc attcttgtg gcaagttcg atgggcttt 
      gcctccagt ctggtctat gtctccagc aaatacaaa 
      gaagccgcc gaaaacgtt caaattttg atcggctta 
      gctccggtt tggagcctg aaatataca aaaagtttg 
      tacaaatgg gctcttttc ccctccatc cactccata 
      cttaaaaat gggctaaac cgctttatt agttggcac 
      agttggcca cattatgac gggtggatg ctgtggact 
      ttgagaatg ttgaacaaa gttttccct ttcaaaaaa 
      ctgtacatc tacactgaa tccgtgttt tgtggctgg 
      agcgaaacc gggtttgat cctagtttc ataaacttg 
      atgattgat catctcgga gctccatct tctttgaaa 
      aattttgaa cacatttat cagactata aacaatgaa 
      gaagtccgt atgtttgat tatggaaaa agcagaaat 
      ttggaaatt tatggctcg gaaatgaac ccgtcctat 
      ctgttagaa aatgtttca gttcctctt cttttagtt 
      tatggcact aatgactac ttaacccaa gctggcgat 
      atcgacgcc ttaactcag aaactccct gaggaggcc 
      aaagttttt ggagaaatg aagattgag ggattttct 
      catacagat ttttacttt ggcaggcac agatacgaa 
      atggtttac aagaaagtg attgaattt cttggcaat 
      ttggaggaa taa
    mRNA sequence
    • >TC032783 RA
      ccgtaagaa ttatgatta ttttcctga aaattctca 
      tttttgcac ttcctctca ccacttgcc agttaaata 
      atgcctgca aaagcttcg aaagctatt tcaatatct 
      atttcagtc ccgactgtt tttacattc ctgataact 
      acttaaacg cccccgaaa taatcaaac atcatgtag 
      gcttattcg aacaccaca aagttacca ccgaagatg 
      gctacattt tgggcctct ttcggattc cacaaacta 
      gcccaaagg gcgtaattc tcttgcagc acggttttg 
      tgcaagacg ccaggtctt ggttgtctc agtacaacg 
      aatcggtgg ctttctggt tctggaagg ccggttacg 
      atgtgtggc tttccaaca gtcggggca ctttttact 
      cgcaaaaac attcaaatc tcacagtta atgatgagg 
      agttttgga attttacgt ttcacgaaa ttggctatt 
      ttgatttgg atgctgtta tcaagtatg tgaaagtgt 
      gcactaaac gtcccaagg tcattcttg tggcaagtt 
      cgatgggct ttgcctcca gtctggtct atgtctcca 
      gcaaataca aagaagccg ccgaaaacg ttcaaattt 
      tgatcggct tagctccgg tttggagcc tgaaatata 
      caaaaagtt tgtacaaat gggctcttt tcccctcca 
      tccactcca tacttaaaa atgggctaa accgcttta 
      ttagttggc acagttggc cacattatg acgggtgga 
      tgctgtgga ctttgagaa tgttgaaca aagttttcc 
      ctttcaaaa aactgtaca tctacactg aatccgtgt 
      tttgtggct ggagcgaaa ccgggtttg atcctagtt 
      tcataaact tgatgattg atcatctcg gagctccat 
      cttctttga aaaattttg aacacattt atcagacta 
      taaacaatg aagaagtcc gtatgtttg attatggaa 
      aaagcagaa atttggaaa tttatggct cggaaatga 
      acccgtcct atctgttag aaaatgttt cagttcctc 
      ttcttttag tttatggca ctaatgact acttaaccc 
      aagctggcg atatcgacg ccttaactc agaaactcc 
      ctgaggagg ccaaagttt ttggagaaa tgaagattg 
      agggatttt ctcatacag atttttact ttggcaggc 
      acagatacg aaatggttt acaagaaag tgattgaat 
      ttcttggca atttggagg aataacaaa accatcgca 
      aaatgacat aacatgttg tagaagtaa atattataa 
      aaccaaaga attgttttt actttattc ttgtttcat 
      tatttttta catcaggca ggagaaaga aaatctcaa 
      aaactaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY