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Gene information
General information
TC007612 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 2289717..2291440
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007612
    iBeetle screen iB_02756

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    head segment random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007612 RA
      MEISQTELD LSKVCHACL AVDTKVQQF TESDADHAH 
      ILPIFKEFT SLELNDYGN IPLFFCNTC VLKVEEFYS 
      FKEMCLKSH DTILTILKE NVIVCEEEK EEIPLVQFE 
      TISEDTEIV KKEANTEMQ SKDLDLHSN EEEGEKRPF 
      MCVICREGF KNTEEFTKH NDLVHCTND YKCSLCSEG 
      FTNPFLLTE HLKIHKNTK TAHNVYSCG RCDNTYKTL 
      QGLQVHVYS HLGLRPYKC SVCPKSFTK PTALKTHED 
      LHSGVRKFT CTECGKGYS YKLELRRHQ VVHTGLRPF 
      KCEQCPTTF AFVRDLTIH MRIHTGEKP YLCPTCGKA 
      FRRIYHLTV HKRIHTGEK PHKCSFCEK SFAQKGDWK 
      AHERLHTGV KPYVCQTCD KSFTQSSAL TTHMRTHEV 
      KQWDCQVCS RTFHRQGSY KKHMGAHKR AHNI
    CDS sequence
    • >TC007612 RA
      atggaaatc tcgcagact gaactagat ttaagtaaa 
      gtttgccac gcgtgtctt gctgtggac acaaaagtg 
      caacaattc actgaatca gatgccgac catgcccac 
      attttgccg attttcaaa gagttcacc tctttggag 
      ttgaatgac tacggaaac ataccttta tttttttgc 
      aacacatgt gtacttaaa gtggaagaa ttttattcg 
      tttaaagaa atgtgtctc aagtctcat gatacaatt 
      cttacaatt ttaaaagag aacgtaatt gtctgtgag 
      gaggaaaaa gaagaaata cctttggta cagtttgag 
      acaattagt gaagataca gaaattgtc aaaaaagag 
      gccaataca gagatgcag agcaaagat ttagatttg 
      cattctaat gaagaagaa ggggaaaaa cgtccgttt 
      atgtgcgtg atttgtcgc gaaggcttc aaaaacaca 
      gaagagttc accaaacac aatgacctg gttcactgc 
      acaaatgac tacaagtgt tcactttgt tccgaaggt 
      ttcaccaac cctttcctc ctgactgaa cacctaaaa 
      atccacaaa aacacaaaa accgcccac aatgtttac 
      agctgcggc agatgcgac aacacgtac aaaaccctt 
      caaggcctc caagtccac gtgtattcg catctcgga 
      ctgcgcccc tacaaatgc tctgtctgc cctaaaagc 
      ttcaccaaa cccacggcc ttgaaaacc cacgaagac 
      ctccattca ggcgtccgc aaattcaca tgcacagaa 
      tgtggcaaa ggttacagc tacaagctg gaattgcgc 
      cgccaccaa gtcgtccac acggggcta cggcccttc 
      aaatgcgaa cagtgcccc accacgttc gcattcgtg 
      cgcgattta acaattcac atgaggatc cacacgggg 
      gaaaaaccc tacttgtgc ccaacgtgt ggcaaagct 
      ttcagacga atttaccac ctcacagtc cacaaacgc 
      atacacacg ggggaaaaa ccccacaag tgtagtttc 
      tgcgagaag agtttcgcc caaaaaggc gactggaag 
      gcccacgag aggctgcac acgggggtg aagccctat 
      gtgtgtcaa acgtgcgac aagagtttc acccaatct 
      agtgcttta actactcat atgagaacg cacgaggtg 
      aagcagtgg gattgccag gtttgtagt aggactttt 
      cacagacag gggagttat aaaaaacat atgggggca 
      cataagagg gcccacaac atttaa
    mRNA sequence
    • >TC007612 RA
      aaaaaattg gtttttaat ttttttaaa aacacgaac 
      agaaatgga aatctcgca gactgaact agatttaag 
      taaagtttg ccacgcgtg tcttgctgt ggacacaaa 
      agtgcaaca attcactga atcagatgc cgaccatgc 
      ccacatttt gccgatttt caaagagtt cacctcttt 
      ggagttgaa tgactacgg aaacatacc tttattttt 
      ttgcaacac atgtgtact taaagtgga agaatttta 
      ttcgtttaa agaaatgtg tctcaagtc tcatgatac 
      aattcttac aattttaaa agagaacgt aattgtctg 
      tgaggagga aaaagaaga aataccttt ggtacagtt 
      tgagacaat tagtgaaga tacagaaat tgtcaaaaa 
      agaggccaa tacagagat gcagagcaa agatttaga 
      tttgcattc taatgaaga agaagggga aaaacgtcc 
      gtttatgtg cgtgatttg tcgcgaagg cttcaaaaa 
      cacagaaga gttcaccaa acacaatga cctggttca 
      ctgcacaaa tgactacaa gtgttcact ttgttccga 
      aggtttcac caacccttt cctcctgac tgaacacct 
      aaaaatcca caaaaacac aaaaaccgc ccacaatgt 
      ttacagctg cggcagatg cgacaacac gtacaaaac 
      ccttcaagg cctccaagt ccacgtgta ttcgcatct 
      cggactgcg cccctacaa atgctctgt ctgccctaa 
      aagcttcac caaacccac ggccttgaa aacccacga 
      agacctcca ttcaggcgt ccgcaaatt cacatgcac 
      agaatgtgg caaaggtta cagctacaa gctggaatt 
      gcgccgcca ccaagtcgt ccacacggg gctacggcc 
      cttcaaatg cgaacagtg ccccaccac gttcgcatt 
      cgtgcgcga tttaacaat tcacatgag gatccacac 
      gggggaaaa accctactt gtgcccaac gtgtggcaa 
      agctttcag acgaattta ccacctcac agtccacaa 
      acgcataca cacggggga aaaacccca caagtgtag 
      tttctgcga gaagagttt cgcccaaaa aggcgactg 
      gaaggccca cgagaggct gcacacggg ggtgaagcc 
      ctatgtgtg tcaaacgtg cgacaagag tttcaccca 
      atctagtgc tttaactac tcatatgag aacgcacga 
      ggtgaagca gtgggattg ccaggtttg tagtaggac 
      ttttcacag acaggggag ttataaaaa acatatggg 
      ggcacataa gagggccca caacattta attgtattt 
      aggcgaaac gaatttcac atttttgtg accgctaaa 
      gtcggttaa atacagttt cggtttgta ttcattttt 
      cactttata tcgggaaaa aattgcttc aaatatttg 
      ataaacaaa ctcgattac gctcaggat cgaacctcc 
      cagacacag gccgatgag gccgccaac ggacgctga 
      aaatctttg ttactaaaa gttcgtcca aggacttaa 
      cactaggag gcgattttg atttaaaat ctctcaaaa 
      aattaatgt tgtttgtta aatatagcc ctttctaac 
      ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY