Status
Please wait ...
Gene information
General information
TC032748
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030764,TC007657
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 1421264..1427089
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032748
    iBeetle screen iB_02754
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032748 RA
      MSPERSNSP QDEAVMTPK MSVPASPVA GTPIREAQS 
      PISLLLAVA QSQSRNGDD DLTSLSWLH ERDLLKGMN 
      INPSPSNSV NSTPVKYSN SLLSDQFPT SDYVDDSSI 
      SAADSSSSS LNSPVPYNN SSQRNKHPH NVPYDPLVH 
      TNNKPPYSF SCLIFMAIE DSPQKALPV KEIYAWILE 
      HFPYFKNAP TGWKNSVRH NLSLNKCFQ KVEKAPNLG 
      KGSLWTVDQ QYKPNLIQA LTRSPFHPC STLDPSTYF 
      NNNNKSNLT PEKSSMSRL PNPELYPYL SRKLASAEL 
      NNRNIKSEN SDESLDAVD AAAVMLSLK NGPRYRQKK 
      EKALYCQVI TTSPSQDHT YSAADSVDP LGTDEAFES 
      DDERTFRVV KSRTCRKID FEDEEERKI KEGAETLLN 
      LAGITTRKR FNSQSSDYE PYKRIKLSP QYDINDNEA 
      TERPTPFKP RLLRAKKKE GKSKMLCNN NDEWVRHRR 
      ELEINQHR
    • >TC032748 RB
      MATVTLWVA ALIPDGRLA SDACERETF RCEVGAGVW 
      DAFRTGTMS PERSNSPQD EAVMTPKMS VPASPVAGT 
      PIREAQSPI SLLLAVAQS QSRNGDDDL TSLSWLHER 
      DLLKGMNIN PSPSNSVNS TPVKYSNSL LSDQFPTSD 
      YVDDSSISA ADSSSSSLN SPVPYNNSS QRNKHPHNV 
      PYDPLVHTN NKPPYSFSC LIFMAIEDS PQKALPVKE 
      IYAWILEHF PYFKNAPTG WKNSVRHNL SLNKCFQKV 
      EKAPNLGKG SLWTVDQQY KPNLIQALT RSPFHPCST 
      LDPSTYFNN NNKSNLTPE KSSMSRLPN PELYPYLSR 
      KLASAELNN RNIKSENSD ESLDAVDAA AVMLSLKNG 
      PRYRQKKEK ALYCQVITT SPSQDHTYS AADSVDPLG 
      TDEAFESDD ERTFRVVKS RTCRKIDFE DEEERKIKE 
      GAETLLNLA GITTRKRFN SQSSDYEPY KRIKLSPQY 
      DINDNEATE RPTPFKPRL LRAKKKEGK SKMLCNNND 
      EWVRHRREL EINQHR
    CDS sequence
    • >TC032748 RA
      atgtctccc gaacgatca aattcaccc caagatgaa 
      gcagtaatg acaccaaag atgtcagta cccgcaagt 
      cccgtcgca gggacaccc atacgcgaa gcccaatcg 
      cccatatca ttactttta gcagtggct caaagtcaa 
      agcaggaac ggtgatgac gacctcacg agtctctca 
      tggctgcac gaacgcgac cttctgaaa ggaatgaac 
      ataaacccg tcaccgtcg aatagcgtc aattccaca 
      ccagtgaaa tatagcaat agtctcttg tcagatcag 
      tttccgacg agtgattac gtcgatgat tcttccata 
      tcagcagct gactcgtcg agtagttcc ctaaactcg 
      cccgttccg tacaacaac agttcccaa aggaacaaa 
      catccacat aatgtacct tacgaccct ttggttcac 
      acaaacaac aagccacca tattccttc tcctgtctc 
      attttcatg gcgattgaa gattcgccc cagaaagct 
      cttccagtg aaggagatc tatgcctgg attttggaa 
      cacttcccc tacttcaaa aacgcccca actgggtgg 
      aaaaacagc gtcagacac aacctatcc ctcaacaag 
      tgtttccaa aaagtggaa aaagccccg aacctgggt 
      aaaggttca ctatggact gtcgaccag cagtacaaa 
      cccaactta atccaggcg ctaacgcgc tcacctttc 
      cacccctgt tcaaccctc gacccttcc acttacttc 
      aataacaat aataagagt aatttaact cctgaaaaa 
      agctcaatg tcgcgcttg cctaacccc gaattgtac 
      ccctacctt tcaaggaaa ctagcttca gctgaattg 
      aacaacaga aatattaaa tcggagaat tccgatgag 
      tctttggat gctgttgac gctgctgcg gtcatgtta 
      agtttgaaa aacgggcca cggtaccgc cagaagaaa 
      gaaaaagcg ctttactgt caagtcatc acgacttca 
      ccgagccag gatcacact tacagtgcc gccgattcg 
      gtcgatccc ttaggcacg gacgaagcg ttcgaaagc 
      gacgacgaa aggacgttt agggtcgtc aagagtcgc 
      acgtgtcgc aaaatcgac tttgaggac gaggaggag 
      cgcaagatt aaagaaggc gcagagacg cttctgaat 
      ttggctggg ataacgaca aggaagagg ttcaactcg 
      caaagttcg gactacgaa ccgtacaaa agaataaaa 
      ctgtcgccg caatacgac atcaatgat aacgaggca 
      accgagcga ccgacgccc ttcaaaccg cgccttttg 
      cgagcgaag aaaaaagaa ggcaaatca aaaatgttg 
      tgtaataac aacgatgag tgggtcaga caccggcgc 
      gaactggaa atcaaccag cataggtaa
    • >TC032748 RB
      atggcaacg gtgacgctg tgggtcgcc gcgttaatc 
      cccgacgga cgtctcgcg tcggatgcg tgcgagcgt 
      gagacgttc cgatgcgaa gtaggcgcg ggagtctgg 
      gatgcattt agaacgggt acaatgtct cccgaacga 
      tcaaattca ccccaagat gaagcagta atgacacca 
      aagatgtca gtacccgca agtcccgtc gcagggaca 
      cccatacgc gaagcccaa tcgcccata tcattactt 
      ttagcagtg gctcaaagt caaagcagg aacggtgat 
      gacgacctc acgagtctc tcatggctg cacgaacgc 
      gaccttctg aaaggaatg aacataaac ccgtcaccg 
      tcgaatagc gtcaattcc acaccagtg aaatatagc 
      aatagtctc ttgtcagat cagtttccg acgagtgat 
      tacgtcgat gattcttcc atatcagca gctgactcg 
      tcgagtagt tccctaaac tcgcccgtt ccgtacaac 
      aacagttcc caaaggaac aaacatcca cataatgta 
      ccttacgac cctttggtt cacacaaac aacaagcca 
      ccatattcc ttctcctgt ctcattttc atggcgatt 
      gaagattcg ccccagaaa gctcttcca gtgaaggag 
      atctatgcc tggattttg gaacacttc ccctacttc 
      aaaaacgcc ccaactggg tggaaaaac agcgtcaga 
      cacaaccta tccctcaac aagtgtttc caaaaagtg 
      gaaaaagcc ccgaacctg ggtaaaggt tcactatgg 
      actgtcgac cagcagtac aaacccaac ttaatccag 
      gcgctaacg cgctcacct ttccacccc tgttcaacc 
      ctcgaccct tccacttac ttcaataac aataataag 
      agtaattta actcctgaa aaaagctca atgtcgcgc 
      ttgcctaac cccgaattg tacccctac ctttcaagg 
      aaactagct tcagctgaa ttgaacaac agaaatatt 
      aaatcggag aattccgat gagtctttg gatgctgtt 
      gacgctgct gcggtcatg ttaagtttg aaaaacggg 
      ccacggtac cgccagaag aaagaaaaa gcgctttac 
      tgtcaagtc atcacgact tcaccgagc caggatcac 
      acttacagt gccgccgat tcggtcgat cccttaggc 
      acggacgaa gcgttcgaa agcgacgac gaaaggacg 
      tttagggtc gtcaagagt cgcacgtgt cgcaaaatc 
      gactttgag gacgaggag gagcgcaag attaaagaa 
      ggcgcagag acgcttctg aatttggct gggataacg 
      acaaggaag aggttcaac tcgcaaagt tcggactac 
      gaaccgtac aaaagaata aaactgtcg ccgcaatac 
      gacatcaat gataacgag gcaaccgag cgaccgacg 
      cccttcaaa ccgcgcctt ttgcgagcg aagaaaaaa 
      gaaggcaaa tcaaaaatg ttgtgtaat aacaacgat 
      gagtgggtc agacaccgg cgcgaactg gaaatcaac 
      cagcatagg taa
    mRNA sequence
    • >TC032748 RA
      tgtttgggt ggcataaaa tggtgtgct cttgaacct 
      tttgtggaa agtggtata gttttttaa catttttag 
      tgataagta attaaagta caatgtctc ccgaacgat 
      caaattcac cccaagatg aagcagtaa tgacaccaa 
      agatgtcag tacccgcaa gtcccgtcg cagggacac 
      ccatacgcg aagcccaat cgcccatat cattacttt 
      tagcagtgg ctcaaagtc aaagcagga acggtgatg 
      acgacctca cgagtctct catggctgc acgaacgcg 
      accttctga aaggaatga acataaacc cgtcaccgt 
      cgaatagcg tcaattcca caccagtga aatatagca 
      atagtctct tgtcagatc agtttccga cgagtgatt 
      acgtcgatg attcttcca tatcagcag ctgactcgt 
      cgagtagtt ccctaaact cgcccgttc cgtacaaca 
      acagttccc aaaggaaca aacatccac ataatgtac 
      cttacgacc ctttggttc acacaaaca acaagccac 
      catattcct tctcctgtc tcattttca tggcgattg 
      aagattcgc cccagaaag ctcttccag tgaaggaga 
      tctatgcct ggattttgg aacacttcc cctacttca 
      aaaacgccc caactgggt ggaaaaaca gcgtcagac 
      acaacctat ccctcaaca agtgtttcc aaaaagtgg 
      aaaaagccc cgaacctgg gtaaaggtt cactatgga 
      ctgtcgacc agcagtaca aacccaact taatccagg 
      cgctaacgc gctcacctt tccacccct gttcaaccc 
      tcgaccctt ccacttact tcaataaca ataataaga 
      gtaatttaa ctcctgaaa aaagctcaa tgtcgcgct 
      tgcctaacc ccgaattgt acccctacc tttcaagga 
      aactagctt cagctgaat tgaacaaca gaaatatta 
      aatcggaga attccgatg agtctttgg atgctgttg 
      acgctgctg cggtcatgt taagtttga aaaacgggc 
      cacggtacc gccagaaga aagaaaaag cgctttact 
      gtcaagtca tcacgactt caccgagcc aggatcaca 
      cttacagtg ccgccgatt cggtcgatc ccttaggca 
      cggacgaag cgttcgaaa gcgacgacg aaaggacgt 
      ttagggtcg tcaagagtc gcacgtgtc gcaaaatcg 
      actttgagg acgaggagg agcgcaaga ttaaagaag 
      gcgcagaga cgcttctga atttggctg ggataacga 
      caaggaaga ggttcaact cgcaaagtt cggactacg 
      aaccgtaca aaagaataa aactgtcgc cgcaatacg 
      acatcaatg ataacgagg caaccgagc gaccgacgc 
      ccttcaaac cgcgccttt tgcgagcga agaaaaaag 
      aaggcaaat caaaaatgt tgtgtaata acaacgatg 
      agtgggtca gacaccggc gcgaactgg aaatcaacc 
      agcataggt aatcaaacg cccggtaat tagatgaat 
      ataacgtaa ttttaacta agttgaatt cttctaacg 
      ctgttattg taattttaa tatatttta tgtttttgt 
      ttacattta tgaaatatg aagagtaac tatgttagg 
      attgtttta gaagtgtta ctgactgaa aattttgat 
      ttatgacgg ttccacggc aattttatt tctgttgcc 
      gacttaatt ttttttcaa atactccag agcccgaac 
      tgttgtaac atttcgcgt caggttgat caccaaata 
      ttaatttat tctaggagt aatttccgc aagatttta 
      tcgctgatt gaaatttta tctaaatga attaatttt 
      atcactgtt accaaagta ggctaacta atcattaag 
      tggggaagt aatttatat gtatattga ttttagcta 
      attctgttg ttttattta tttatttcc atatttaag 
      ctgttgatt tgtcttaga tgctccacc gcgctgaaa 
      ttcgctgta tttatcctg agttggggc attttggtg 
      tttgatatc tggtcattt cattgaaaa gcaataaac 
      gtgtatcag tgataggga ataagaaaa aatcattta 
      gaattttcc tattgctac aagttgagt ttataatca 
      ttgaaagta gggtagagt aaatatatt ttttctatt 
      tcccgtgat acatactgc tcttcctat tgtacataa 
      agatccgaa acaatcata aatgtttca aaatgtgtg 
      tgcgtttca aagctattt tcctattac tcttatcat 
      tcaatttct ccaactttt caaaaagag ttttattgt 
      atttttggc cgtcttccg aaattgaat gaataagtg 
      cgatacttg attttgtta gtttagaat gtgagaatt 
      ctaaatatc ttatagcaa taccagttt tgcctgatc 
      tagtaaata atactctat gctttgcca atattcttg 
      gctttggta tgtattttt aaggttcta tgtgccaaa 
      tcgttttgt acttttatt gcagtaatc actactggc 
      aatattttt tggcgcctg tcttccaat tttataaaa 
      taggtcttc tgacatact ttccatatt tacattttt 
      agtaggtta gactttcgt tcaaatgtg ttccattaa 
      caaactatt agtgtgctt ttgactttt tccttattt 
      tcaaacgaa cttaacact tgtttgcgc atattttat 
      tttattatt gttatgacc atttatttg tacctttag 
      tttattcaa ataaataaa acggctgat tttaa
    • >TC032748 RB
      tgtttgggt ggcataaaa tggtgtgct cttgaacct 
      tttgtggaa agtggtata gttttttaa catttttag 
      tgataaggt aaacttcat tacgtcagc ttctcatgg 
      gttgctatg gcaacggtg acgctgtgg gtcgccgcg 
      ttaatcccc gacggacgt ctcgcgtcg gatgcgtgc 
      gagcgtgag acgttccga tgcgaagta ggcgcggga 
      gtctgggat gcatttaga acgggtaca atgtctccc 
      gaacgatca aattcaccc caagatgaa gcagtaatg 
      acaccaaag atgtcagta cccgcaagt cccgtcgca 
      gggacaccc atacgcgaa gcccaatcg cccatatca 
      ttactttta gcagtggct caaagtcaa agcaggaac 
      ggtgatgac gacctcacg agtctctca tggctgcac 
      gaacgcgac cttctgaaa ggaatgaac ataaacccg 
      tcaccgtcg aatagcgtc aattccaca ccagtgaaa 
      tatagcaat agtctcttg tcagatcag tttccgacg 
      agtgattac gtcgatgat tcttccata tcagcagct 
      gactcgtcg agtagttcc ctaaactcg cccgttccg 
      tacaacaac agttcccaa aggaacaaa catccacat 
      aatgtacct tacgaccct ttggttcac acaaacaac 
      aagccacca tattccttc tcctgtctc attttcatg 
      gcgattgaa gattcgccc cagaaagct cttccagtg 
      aaggagatc tatgcctgg attttggaa cacttcccc 
      tacttcaaa aacgcccca actgggtgg aaaaacagc 
      gtcagacac aacctatcc ctcaacaag tgtttccaa 
      aaagtggaa aaagccccg aacctgggt aaaggttca 
      ctatggact gtcgaccag cagtacaaa cccaactta 
      atccaggcg ctaacgcgc tcacctttc cacccctgt 
      tcaaccctc gacccttcc acttacttc aataacaat 
      aataagagt aatttaact cctgaaaaa agctcaatg 
      tcgcgcttg cctaacccc gaattgtac ccctacctt 
      tcaaggaaa ctagcttca gctgaattg aacaacaga 
      aatattaaa tcggagaat tccgatgag tctttggat 
      gctgttgac gctgctgcg gtcatgtta agtttgaaa 
      aacgggcca cggtaccgc cagaagaaa gaaaaagcg 
      ctttactgt caagtcatc acgacttca ccgagccag 
      gatcacact tacagtgcc gccgattcg gtcgatccc 
      ttaggcacg gacgaagcg ttcgaaagc gacgacgaa 
      aggacgttt agggtcgtc aagagtcgc acgtgtcgc 
      aaaatcgac tttgaggac gaggaggag cgcaagatt 
      aaagaaggc gcagagacg cttctgaat ttggctggg 
      ataacgaca aggaagagg ttcaactcg caaagttcg 
      gactacgaa ccgtacaaa agaataaaa ctgtcgccg 
      caatacgac atcaatgat aacgaggca accgagcga 
      ccgacgccc ttcaaaccg cgccttttg cgagcgaag 
      aaaaaagaa ggcaaatca aaaatgttg tgtaataac 
      aacgatgag tgggtcaga caccggcgc gaactggaa 
      atcaaccag cataggtaa tcaaacgcc cggtaatta 
      gatgaatat aacgtaatt ttaactaag ttgaattct 
      tctaacgct gttattgta attttaata tattttatg 
      tttttgttt acatttatg aaatatgaa gagtaacta 
      tgttaggat tgttttaga agtgttact gactgaaaa 
      ttttgattt atgacggtt ccacggcaa ttttatttc 
      tgttgccga cttaatttt ttttcaaat actccagag 
      cccgaactg ttgtaacat ttcgcgtca ggttgatca 
      ccaaatatt aatttattc taggagtaa tttccgcaa 
      gattttatc gctgattga aattttatc taaatgaat 
      taattttat cactgttac caaagtagg ctaactaat 
      cattaagtg gggaagtaa tttatatgt atattgatt 
      ttagctaat tctgttgtt ttatttatt tatttccat 
      atttaagct gttgatttg tcttagatg ctccaccgc 
      gctgaaatt cgctgtatt tatcctgag ttggggcat 
      tttggtgtt tgatatctg gtcatttca ttgaaaagc 
      aataaacgt gtatcagtg atagggaat aagaaaaaa 
      tcatttaga attttccta ttgctacaa gttgagttt 
      ataatcatt gaaagtagg gtagagtaa atatatttt 
      ttctatttc ccgtgatac atactgctc ttcctattg 
      tacataaag atccgaaac aatcataaa tgtttcaaa 
      atgtgtgtg cgtttcaaa gctattttc ctattactc 
      ttatcattc aatttctcc aacttttca aaaagagtt 
      ttattgtat ttttggccg tcttccgaa attgaatga 
      ataagtgcg atacttgat tttgttagt ttagaatgt 
      gagaattct aaatatctt atagcaata ccagttttg 
      cctgatcta gtaaataat actctatgc tttgccaat 
      attcttggc tttggtatg tatttttaa ggttctatg 
      tgccaaatc gttttgtac ttttattgc agtaatcac 
      tactggcaa tattttttg gcgcctgtc ttccaattt 
      tataaaata ggtcttctg acatacttt ccatattta 
      catttttag taggttaga ctttcgttc aaatgtgtt 
      ccattaaca aactattag tgtgctttt gactttttc 
      cttattttc aaacgaact taacacttg tttgcgcat 
      attttattt tattattgt tatgaccat ttatttgta 
      cctttagtt tattcaaat aaataaaac ggctgattt 
      taa