| General information | |||
| TC007672 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 1077776..1092065 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007672 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02753 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC007672 RA
MTSVVFLLG AALFGSVKL VQTAELVIH IPGNLGQEG VIYRLDYYP PFGHPAPNT TIASKDIQD VIKFSQALP GTKYDFWLY YSNSTHNTL TWTANFTTV PDPPSNLSV SVRSGKSAV ISWSPPTQG SYSSFKLKI NCLNNVPEA KPQTLTIDH VDNNSYLLK ELTPGATYQ VQAFTVFEN KESAAYTSR NFTTKPNTP GKFIVWFRN ETTLLVLWQ PPYPAAIYT HYKVSIDPP DANDSILYV EKEGEPPGP AQAAFKGLV PGRAYNISV QTMSEDEIS APTTAQYRT VPLRPSKIT FRLVTSYSF EVHWEEPNG KSEFDKYHV FVGTTKSKQ QITKMRDDP TFYEFSENL DPGKTYSVT VKTVSGKVS SWPATAEVT LKPLPVMHV HAESDIKNG IVNISWSPN PHSFQDNYL VSYHEVESS TGDSNNIKT NNKTKITLD TLLPGRNYS LIVQALSKD MESNESYLY VVTQPSAPI IEDLRPMVD GLNISWKSD VNSRQDKYE LHCVRNDTN DKIIRTTHD SRIILSNLY PGAGYLIKV FAISHGLKS EPHEYFQPV FPRPPKNMT IEKTTTTSV LVQWLAPTD SLISEYAIR YRTDTNRNW VRLPAVQNT EAEVTDMTP GERYTIQVN TVSYGLESN EPQQMNHTV RPNPASNIA RLVDSNNVT LEWPRPEGR VETYVVRWW ESGNESEIY SRNVSQSEN GTGPVRLLV SDLMPGVEY TFDIKAISN GLESDKTVL TIRTMPLIQ SEVVVVTNQ QSTDTISLR YTPTPSASS KFDFYRFSL SDTNIPDQE KAWNDTDRF VTFTGLVPG RLYNITVWT VSQGVESQP LQRQDRLYP EPITGINAT SISDTEITL SWASPRGEF NAFEVQYLT FENTLIHNL TLQNTITIT ELKPHRNYT FTVMVRSGT ESSILRQSS PVSGIFTTK ESVPGRVER FEPFDIQPS SISFEWSLP QTEQNGVIR KFTITYGLE GSSNTQSLD FKPNEFKGK IRQLLPGKT YVFRIQGET KVGFGPERV WKQKMPIRE PPVPNSRAV PTEVRRSST TIQIRFRKN YFSDVNGPV TTYAIIVAE DYSKNASGL EMPSWRDVQ AYSVWPPYQ VSEPYFPFF NSSVEDFTI GAESCDIRQ VGYCNGALK SGSTYRVKI RAFTAPDKF TDTGYSFPI QTDQDNTNI ILGVLIPLI LFGILFLVI IYIRRRRSA RKATDTRTN DNMSLPDSV IETSRPVRI DSFANHYRL MSADSDFRF SEEFEELKH VGRDQPATF ADLPCNRPK NRFTNILPY DHSRFKLQP VDDEEGSDY INANYVPGY NSPREFIVT QGPLHSTRD DFWRMVWES NSRAIIMLT RCVEKGREK CDHYWPYES MPVYYGDIS VQILNETRY PDWNISEFM VCRGDQQRV IRHFHFTTW PDFGVPNPP HTLVRFVRA FRERVGSDQ RPIVVHCSA GVGRSGTFI CLDRILQQI LVSDYVDIF GIVYAMRKE RVWMVQTEQ QYICIHQCL LAVLEGKEL SGSPREIHE NQGFEDDEG IAESGM
- >TC007672 RB
MTSVVFLLG AALFGSVKL VQTAELVIH IPGNLGQEG VIYRLDYYP PFGHPAPNT TIASKDIQD VIKFSQALP GTKYDFWLY YSNSTHNTL TWTANFTTV PDPPSNLSV SVRSGKSAV ISWSPPTQG SYSSFKLKI NCLNNVPEA KPQTLTIDH VDNNSYLLK ELTPGATYQ VQAFTVFEN KESAAYTSR NFTTKPNTP GKFIVWFRN ETTLLVLWQ PPYPAAIYT HYKVSIDPP DANDSILYV EKEGEPPGP AQAAFKGLV PGRAYNISV QTMSEDEIS APTTAQYRT VPLRPSKIT FRLVTSYSF EVHWEEPNG KSEFDKYHV FVGTTKSKQ QITKMRDDP TFYEFSENL DPGKTYSVT VKTVSGKVS SWPATAEVT LKPLPVMHV HAESDIKNG IVNISWSPN PHSFQDNYL VSYHEVESS TGDSNNIKT NNKTKITLD TLLPGRNYS LIVQALSKD MESNESYLY VVTQPSAPI IEDLRPMVD GLNISWKSD VNSRQDKYE LHCVRNDTN DKIIRTTHD SRIILSNLY PGAGYLIKV FAISHGLKS EPHEYFQPV FPRPPKNMT IEKTTTTSV LVQWLAPTD SLISEYAIR YRTDTNRNW VRLPAVQNT EAEVTDMTP GERYTIQVN TVSYGLESN EPQQMNHTV RPNPASNIA RLVDSNNVT LEWPRPEGR VETYVVRWW ESGNESEIY SRNVSQSEN GTGPVRLLV SDLMPGVEY TFDIKAISN GLESDKTVL TIRTMPLIQ SEVVVVTNQ QSTDTISLR YTPTPSASS KFDFYRFSL SDTNIPDQE KAWNDTDRF VTFTGLVPG RLYNITVWT VSQGVESQP LQRQDRLYP EPITGINAT SISDTEITL SWASPRGEF NAFEVQYLT FENTLIHNL TLQNTITIT ELKPHRNYT FTVMVRSGT ESSILRQSS PVSGIFTTK ESVPGRVER FEPFDIQPS SISFEWSLP QTEQNGVIR KFTITYGLE GSSNTQSLD FKPNEFKGK IRQLLPGKT YVFRIQGET KVGFGPERV WKQKMPIRE PPVPNSRAV PTEVRRSST TIQIRFRKN YFSDVNGPV TTYAIIVAE DYSKNASGL EMPSWRDVQ AYSVWPPYQ VSEPYFPFF NSSVEDFTI GAESCDIRQ VGYCNGALK SGSTYRVKI RAFTAPDKF TDTGYSFPI QTDQDNTNI ILGVLIPLI LFGILFLVI IYIRRRRSA RKATDTRTN DNMSLPDSV IETSRPVRI DSFANHYRL MSADSDFRF SEEFEELKH VGRDQPATF ADLPCNRPK NRFTNILPY DHSRFKLQP VDDEEGSDY INANYVPGY NSPREFIVT QGPLHSTRD DFWRMVWES NSRAIIMLT RCVEKGREK CDHYWPYES MPVYYGDIS VQILNETRY PDWNISEFM VCRGDQQRV IRHFHFTTW PDFGVPNPP HTLVRFVRA FRERVGSDQ RPIVVHCSA GVGRSGTFI CLDRILQQI LVSDYVDIF GIVYAMRKE RVWMVQTEQ QYICIHQCL LAVLEGKEL SGSPREIHE NQGFEGELV EGFVNFRNL EL
- >TC007672 RA
atgaccagt gtcgttttt ttgcttggt gcagcttta ttcgggtca gtcaagttg gtgcaaacg gcggaactt gtcatccac attccgggc aatttgggc caagaaggg gtgatttac cgcctggac tactacccc cctttcggc caccccgcc cccaacacc accatcgcc tccaaagac atccaggac gtgatcaag ttctcgcaa gccttacca ggcaccaag tacgacttc tggctctat tactccaac tccacgcac aatacgctc acctggact gcgaatttt acaaccgtc ccggacccg ccctcgaat ctctcggtt agcgtccgc agtggtaaa tctgcggtg atttcgtgg agtccgcca acacaaggc agctactcc agcttcaaa ctcaaaatc aactgtctg aataacgta ccagaggcc aaaccccaa actctgaca attgaccat gttgataac aactcctac ctcctcaag gagctcaca ccgggggcc acctatcaa gtgcaagcg ttcacggtg tttgaaaat aaggaaagt gcagcctac acgtcacga aattttact acaaaaccg aacactccg gggaaattt atcgtgtgg ttccggaac gagacaact ttgcttgtt ttatggcag cccccttat cctgccgct atttacacg cattataaa gtctcaatt gaccctcct gatgctaat gacagtata ctgtacgtg gagaaagag ggtgagcct ccagggccg gctcaggcg gcctttaag ggcttggtg ccaggccgg gcttacaat atttcggta caaacgatg tcagaagat gagatttcg gcacctacc actgctcaa taccgaact gtgccttta aggccctcc aaaataacc tttagactc gttacttcg tattcgttt gaagtccac tgggaggaa cccaatggt aaaagtgag ttcgataaa taccacgtt tttgtggga acgacgaaa agtaagcaa cagatcacg aaaatgcgc gacgatccc acgttttac gagttcagc gaaaaccta gaccctgga aaaacctac agtgtgact gttaaaaca gtctctggg aaagtctcc tcctggcct gctacggcc gaagtgact ttaaagccg cttcctgtg atgcatgtg catgccgaa agtgatatt aaaaacggc attgttaac atatcatgg agtccgaac ccgcacagc ttccaggac aactacttg gtgtcgtac cacgaagtc gagagttcg accggtgat agtaacaat ataaagaca aataacaag accaaaata acgctcgat acgcttctg cctggtcgg aattattca ctcattgtc caagcgctc tctaaagat atggagtcg aatgagagt tatctctat gtggttaca caaccaagc gcgcccata attgaagac ctcagaccc atggtcgat ggtctcaac atcagctgg aagagcgat gtcaattca cgccaagac aaatacgaa ttacactgt gtccggaac gacaccaat gataaaatt ataaggaca acccatgac tccaggata atcctctcg aatctttac ccgggagct ggctatctg atcaaagtt ttcgccatt tcgcacggc ctgaagagc gaaccccac gagtatttc cagccagtt ttcccccgt ccgcccaag aacatgaca atcgagaaa accacgacc acttcggtc ctggtccag tggctggcc cccaccgat tcgctcatt tccgaatac gctattcgc taccgcacc gacaccaat agaaattgg gtgcgatta ccagcagtc cagaataca gaagccgaa gttaccgac atgactccc ggcgaacgg tacacgatt caagtgaat acagttagt tacggtttg gaaagtaac gaaccacaa caaatgaat cacacagtt cgaccaaat ccagcatca aacattgca cgtttggtt gattcgaat aatgtgact ctggaatgg ccacgtcct gaaggaaga gtcgaaacg tacgttgtt cgatggtgg gaatccggg aacgaatcg gaaatctac tcccggaat gtgagtcag agcgagaat ggaactggg cctgtgaga ctactggtc agtgattta atgccaggg gttgagtac acgtttgat atcaaagcg atttcgaat ggattggaa agtgacaag acggtgtta acaatcagg acaatgcct ttgatccaa tcagaagtc gtggtggtg accaatcaa caatcgaca gacactata agtttgagg tatacaccg actccttca gcttcgtcc aagttcgat ttttaccga ttttcactg tcggatact aatattcct gaccaggaa aaagcctgg aatgacacg gaccgattt gttactttc accggatta gtgccaggc cgactgtac aatattaca gtatggacg gtcagtcaa ggggtcgaa agtcagccc ttgcagcgc caggataga ctttaccca gaacccatc accggcatc aacgcgacc tccatctcc gacacggaa ataaccctc tcctgggcg tccccacgt ggcgaattc aacgccttt gaagtccaa tacctcacc ttcgaaaac accctcatc cacaacctc accctccag aacaccatc accatcacc gagctcaaa ccccaccgc aactacacg ttcacagtg atggtgcga agcggcacc gagtcgagc attttgcgc caatcaagc ccagtttcg ggcattttc acgaccaag gaatcggtc ccaggccga gtggaacgt ttcgaaccc ttcgatatc caacccagt agcatttcc ttcgagtgg tcgctccct cagaccgag cagaacggc gtcatccgc aagttcacc ataacttac ggactggaa ggctcctcc aacacacaa tcactcgac ttcaagccg aacgaattc aagggaaaa atccggcag ttattaccc gggaaaacc tacgttttc cgcatccaa ggggagacg aaagtcggg tttggacct gagagagtc tggaagcag aaaatgccg atccgggaa ccccccgtt ccgaattcg agggcggtc ccgaccgaa gtgcgccga agctccacg acgattcaa attcggttc aggaagaat tatttcagt gatgtgaac gggcctgtg accacatat gccattatt gtggcggag gattattcg aaaaatgcc tctgggttg gaaatgccc agctggagg gacgttcag gcgtattct gtgtggccg ccgtatcaa gtttcggag ccgtatttt ccgttcttt aacagttcg gttgaggat tttacgatt ggggccgaa agttgcgac atacgacaa gtggggtat tgtaatgga gcgctcaag agtgggtcc acttatcgg gtgaaaata cgggctttt acagcgcct gataagttc actgatacg gggtacagt tttcccatt cagacagat caagacaac acgaatata atactcgga gttttgatt ccactgatc ctcttcggc atcctcttc ttggttatc atctacata agacggaga agatcagct cggaaggcc accgacacg cggacaaac gacaacatg tcgcttccg gattcggta attgagacc agtcgaccg gtccgaatc gacagcttc gccaaccac taccgactg atgtcggcc gattcggac tttcggttc agtgaagag ttcgaagaa ctcaagcat gtggggaga gaccaaccg gcgactttt gccgacctg ccctgcaac aggccgaag aaccgcttc acaaacatc ctgccctac gaccactca aggttcaaa ctccagcca gtggacgac gaagaaggg tccgactac atcaacgcc aactatgtg ccgggctat aactccccg cgcgagttt atagtgacc caagggccg ttacattca acccgtgac gacttctgg cggatggtt tgggagtcg aactcaagg gctattatc atgttgaca cggtgtgtg gaaaaaggg cgcgaaaag tgcgaccat tattggccc tacgagtcc atgcctgtg tactatggg gacatttcg gtgcaaatt ttgaacgaa actcggtat cctgactgg aacatttcc gagttcatg gtgtgtcgg ggtgaccaa cagagggtg atccgccac ttccacttc accacgtgg cctgacttc ggtgtcccg aacccccct cacaccctc gtacgtttc gtccgtgcg tttcgggag agagtgggt tcggatcaa cgcccgatt gttgtccac tgcagtgcc ggagtgggc cgttccggc acgttcatt tgcctggac cgcatcctg cagcagatc ctcgtttcg gactacgtg gacatcttc ggtatcgtg tacgcaatg cgtaaggag cgtgtctgg atggtacag accgaacag cagtacatt tgcatccac cagtgcctt ttggccgtg ctggagggc aaagaactg tccggatca ccgcgtgaa attcacgaa aatcaagga tttgaagat gacgaaggc attgcagag tctgggatg tga
- >TC007672 RB
atgaccagt gtcgttttt ttgcttggt gcagcttta ttcgggtca gtcaagttg gtgcaaacg gcggaactt gtcatccac attccgggc aatttgggc caagaaggg gtgatttac cgcctggac tactacccc cctttcggc caccccgcc cccaacacc accatcgcc tccaaagac atccaggac gtgatcaag ttctcgcaa gccttacca ggcaccaag tacgacttc tggctctat tactccaac tccacgcac aatacgctc acctggact gcgaatttt acaaccgtc ccggacccg ccctcgaat ctctcggtt agcgtccgc agtggtaaa tctgcggtg atttcgtgg agtccgcca acacaaggc agctactcc agcttcaaa ctcaaaatc aactgtctg aataacgta ccagaggcc aaaccccaa actctgaca attgaccat gttgataac aactcctac ctcctcaag gagctcaca ccgggggcc acctatcaa gtgcaagcg ttcacggtg tttgaaaat aaggaaagt gcagcctac acgtcacga aattttact acaaaaccg aacactccg gggaaattt atcgtgtgg ttccggaac gagacaact ttgcttgtt ttatggcag cccccttat cctgccgct atttacacg cattataaa gtctcaatt gaccctcct gatgctaat gacagtata ctgtacgtg gagaaagag ggtgagcct ccagggccg gctcaggcg gcctttaag ggcttggtg ccaggccgg gcttacaat atttcggta caaacgatg tcagaagat gagatttcg gcacctacc actgctcaa taccgaact gtgccttta aggccctcc aaaataacc tttagactc gttacttcg tattcgttt gaagtccac tgggaggaa cccaatggt aaaagtgag ttcgataaa taccacgtt tttgtggga acgacgaaa agtaagcaa cagatcacg aaaatgcgc gacgatccc acgttttac gagttcagc gaaaaccta gaccctgga aaaacctac agtgtgact gttaaaaca gtctctggg aaagtctcc tcctggcct gctacggcc gaagtgact ttaaagccg cttcctgtg atgcatgtg catgccgaa agtgatatt aaaaacggc attgttaac atatcatgg agtccgaac ccgcacagc ttccaggac aactacttg gtgtcgtac cacgaagtc gagagttcg accggtgat agtaacaat ataaagaca aataacaag accaaaata acgctcgat acgcttctg cctggtcgg aattattca ctcattgtc caagcgctc tctaaagat atggagtcg aatgagagt tatctctat gtggttaca caaccaagc gcgcccata attgaagac ctcagaccc atggtcgat ggtctcaac atcagctgg aagagcgat gtcaattca cgccaagac aaatacgaa ttacactgt gtccggaac gacaccaat gataaaatt ataaggaca acccatgac tccaggata atcctctcg aatctttac ccgggagct ggctatctg atcaaagtt ttcgccatt tcgcacggc ctgaagagc gaaccccac gagtatttc cagccagtt ttcccccgt ccgcccaag aacatgaca atcgagaaa accacgacc acttcggtc ctggtccag tggctggcc cccaccgat tcgctcatt tccgaatac gctattcgc taccgcacc gacaccaat agaaattgg gtgcgatta ccagcagtc cagaataca gaagccgaa gttaccgac atgactccc ggcgaacgg tacacgatt caagtgaat acagttagt tacggtttg gaaagtaac gaaccacaa caaatgaat cacacagtt cgaccaaat ccagcatca aacattgca cgtttggtt gattcgaat aatgtgact ctggaatgg ccacgtcct gaaggaaga gtcgaaacg tacgttgtt cgatggtgg gaatccggg aacgaatcg gaaatctac tcccggaat gtgagtcag agcgagaat ggaactggg cctgtgaga ctactggtc agtgattta atgccaggg gttgagtac acgtttgat atcaaagcg atttcgaat ggattggaa agtgacaag acggtgtta acaatcagg acaatgcct ttgatccaa tcagaagtc gtggtggtg accaatcaa caatcgaca gacactata agtttgagg tatacaccg actccttca gcttcgtcc aagttcgat ttttaccga ttttcactg tcggatact aatattcct gaccaggaa aaagcctgg aatgacacg gaccgattt gttactttc accggatta gtgccaggc cgactgtac aatattaca gtatggacg gtcagtcaa ggggtcgaa agtcagccc ttgcagcgc caggataga ctttaccca gaacccatc accggcatc aacgcgacc tccatctcc gacacggaa ataaccctc tcctgggcg tccccacgt ggcgaattc aacgccttt gaagtccaa tacctcacc ttcgaaaac accctcatc cacaacctc accctccag aacaccatc accatcacc gagctcaaa ccccaccgc aactacacg ttcacagtg atggtgcga agcggcacc gagtcgagc attttgcgc caatcaagc ccagtttcg ggcattttc acgaccaag gaatcggtc ccaggccga gtggaacgt ttcgaaccc ttcgatatc caacccagt agcatttcc ttcgagtgg tcgctccct cagaccgag cagaacggc gtcatccgc aagttcacc ataacttac ggactggaa ggctcctcc aacacacaa tcactcgac ttcaagccg aacgaattc aagggaaaa atccggcag ttattaccc gggaaaacc tacgttttc cgcatccaa ggggagacg aaagtcggg tttggacct gagagagtc tggaagcag aaaatgccg atccgggaa ccccccgtt ccgaattcg agggcggtc ccgaccgaa gtgcgccga agctccacg acgattcaa attcggttc aggaagaat tatttcagt gatgtgaac gggcctgtg accacatat gccattatt gtggcggag gattattcg aaaaatgcc tctgggttg gaaatgccc agctggagg gacgttcag gcgtattct gtgtggccg ccgtatcaa gtttcggag ccgtatttt ccgttcttt aacagttcg gttgaggat tttacgatt ggggccgaa agttgcgac atacgacaa gtggggtat tgtaatgga gcgctcaag agtgggtcc acttatcgg gtgaaaata cgggctttt acagcgcct gataagttc actgatacg gggtacagt tttcccatt cagacagat caagacaac acgaatata atactcgga gttttgatt ccactgatc ctcttcggc atcctcttc ttggttatc atctacata agacggaga agatcagct cggaaggcc accgacacg cggacaaac gacaacatg tcgcttccg gattcggta attgagacc agtcgaccg gtccgaatc gacagcttc gccaaccac taccgactg atgtcggcc gattcggac tttcggttc agtgaagag ttcgaagaa ctcaagcat gtggggaga gaccaaccg gcgactttt gccgacctg ccctgcaac aggccgaag aaccgcttc acaaacatc ctgccctac gaccactca aggttcaaa ctccagcca gtggacgac gaagaaggg tccgactac atcaacgcc aactatgtg ccgggctat aactccccg cgcgagttt atagtgacc caagggccg ttacattca acccgtgac gacttctgg cggatggtt tgggagtcg aactcaagg gctattatc atgttgaca cggtgtgtg gaaaaaggg cgcgaaaag tgcgaccat tattggccc tacgagtcc atgcctgtg tactatggg gacatttcg gtgcaaatt ttgaacgaa actcggtat cctgactgg aacatttcc gagttcatg gtgtgtcgg ggtgaccaa cagagggtg atccgccac ttccacttc accacgtgg cctgacttc ggtgtcccg aacccccct cacaccctc gtacgtttc gtccgtgcg tttcgggag agagtgggt tcggatcaa cgcccgatt gttgtccac tgcagtgcc ggagtgggc cgttccggc acgttcatt tgcctggac cgcatcctg cagcagatc ctcgtttcg gactacgtg gacatcttc ggtatcgtg tacgcaatg cgtaaggag cgtgtctgg atggtacag accgaacag cagtacatt tgcatccac cagtgcctt ttggccgtg ctggagggc aaagaactg tccggatca ccgcgtgaa attcacgaa aatcaagga tttgaaggt gagttggtg gagggtttt gtaaatttc cgcaatctc gaactgtaa
- >TC007672 RA
ttgcgtttg ttgtcacgc tggctcgca ggtgctgct ctctactcc ttggcgagg tcctctttg aaagtttcc tcatttcgt ttcgggtta ttgtcaaat gcaataatg gcgtagaag agcgtaacc gtgagtgcg tgtcgtgta atgccatca cggatgcat ctcaattga tacagagcc cccataccg gacataatt atgcctcgt atcccgcgc atcgccccc atttgaatc caccgtacc tgtctcttc tctacccta tattgttat attaaatta tttgtgtgt gtgtatgtg tatgttttt tatttgttg ttgttgttt gttgttgtt attgccatg accagtgtc gtttttttg cttggtgca gctttattc gggtcagtc aagttggtg caaacggcg gaacttgtc atccacatt ccgggcaat ttgggccaa gaaggggtg atttaccgc ctggactac tacccccct ttcggccac cccgccccc aacaccacc atcgcctcc aaagacatc caggacgtg atcaagttc tcgcaagcc ttaccaggc accaagtac gacttctgg ctctattac tccaactcc acgcacaat acgctcacc tggactgcg aattttaca accgtcccg gacccgccc tcgaatctc tcggttagc gtccgcagt ggtaaatct gcggtgatt tcgtggagt ccgccaaca caaggcagc tactccagc ttcaaactc aaaatcaac tgtctgaat aacgtacca gaggccaaa ccccaaact ctgacaatt gaccatgtt gataacaac tcctacctc ctcaaggag ctcacaccg ggggccacc tatcaagtg caagcgttc acggtgttt gaaaataag gaaagtgca gcctacacg tcacgaaat tttactaca aaaccgaac actccgggg aaatttatc gtgtggttc cggaacgag acaactttg cttgtttta tggcagccc ccttatcct gccgctatt tacacgcat tataaagtc tcaattgac cctcctgat gctaatgac agtatactg tacgtggag aaagagggt gagcctcca gggccggct caggcggcc tttaagggc ttggtgcca ggccgggct tacaatatt tcggtacaa acgatgtca gaagatgag atttcggca cctaccact gctcaatac cgaactgtg cctttaagg ccctccaaa ataaccttt agactcgtt acttcgtat tcgtttgaa gtccactgg gaggaaccc aatggtaaa agtgagttc gataaatac cacgttttt gtgggaacg acgaaaagt aagcaacag atcacgaaa atgcgcgac gatcccacg ttttacgag ttcagcgaa aacctagac cctggaaaa acctacagt gtgactgtt aaaacagtc tctgggaaa gtctcctcc tggcctgct acggccgaa gtgacttta aagccgctt cctgtgatg catgtgcat gccgaaagt gatattaaa aacggcatt gttaacata tcatggagt ccgaacccg cacagcttc caggacaac tacttggtg tcgtaccac gaagtcgag agttcgacc ggtgatagt aacaatata aagacaaat aacaagacc aaaataacg ctcgatacg cttctgcct ggtcggaat tattcactc attgtccaa gcgctctct aaagatatg gagtcgaat gagagttat ctctatgtg gttacacaa ccaagcgcg cccataatt gaagacctc agacccatg gtcgatggt ctcaacatc agctggaag agcgatgtc aattcacgc caagacaaa tacgaatta cactgtgtc cggaacgac accaatgat aaaattata aggacaacc catgactcc aggataatc ctctcgaat ctttacccg ggagctggc tatctgatc aaagttttc gccatttcg cacggcctg aagagcgaa ccccacgag tatttccag ccagttttc ccccgtccg cccaagaac atgacaatc gagaaaacc acgaccact tcggtcctg gtccagtgg ctggccccc accgattcg ctcatttcc gaatacgct attcgctac cgcaccgac accaataga aattgggtg cgattacca gcagtccag aatacagaa gccgaagtt accgacatg actcccggc gaacggtac acgattcaa gtgaataca gttagttac ggtttggaa agtaacgaa ccacaacaa atgaatcac acagttcga ccaaatcca gcatcaaac attgcacgt ttggttgat tcgaataat gtgactctg gaatggcca cgtcctgaa ggaagagtc gaaacgtac gttgttcga tggtgggaa tccgggaac gaatcggaa atctactcc cggaatgtg agtcagagc gagaatgga actgggcct gtgagacta ctggtcagt gatttaatg ccaggggtt gagtacacg tttgatatc aaagcgatt tcgaatgga ttggaaagt gacaagacg gtgttaaca atcaggaca atgcctttg atccaatca gaagtcgtg gtggtgacc aatcaacaa tcgacagac actataagt ttgaggtat acaccgact ccttcagct tcgtccaag ttcgatttt taccgattt tcactgtcg gatactaat attcctgac caggaaaaa gcctggaat gacacggac cgatttgtt actttcacc ggattagtg ccaggccga ctgtacaat attacagta tggacggtc agtcaaggg gtcgaaagt cagcccttg cagcgccag gatagactt tacccagaa cccatcacc ggcatcaac gcgacctcc atctccgac acggaaata accctctcc tgggcgtcc ccacgtggc gaattcaac gcctttgaa gtccaatac ctcaccttc gaaaacacc ctcatccac aacctcacc ctccagaac accatcacc atcaccgag ctcaaaccc caccgcaac tacacgttc acagtgatg gtgcgaagc ggcaccgag tcgagcatt ttgcgccaa tcaagccca gtttcgggc attttcacg accaaggaa tcggtccca ggccgagtg gaacgtttc gaacccttc gatatccaa cccagtagc atttccttc gagtggtcg ctccctcag accgagcag aacggcgtc atccgcaag ttcaccata acttacgga ctggaaggc tcctccaac acacaatca ctcgacttc aagccgaac gaattcaag ggaaaaatc cggcagtta ttacccggg aaaacctac gttttccgc atccaaggg gagacgaaa gtcgggttt ggacctgag agagtctgg aagcagaaa atgccgatc cgggaaccc cccgttccg aattcgagg gcggtcccg accgaagtg cgccgaagc tccacgacg attcaaatt cggttcagg aagaattat ttcagtgat gtgaacggg cctgtgacc acatatgcc attattgtg gcggaggat tattcgaaa aatgcctct gggttggaa atgcccagc tggagggac gttcaggcg tattctgtg tggccgccg tatcaagtt tcggagccg tattttccg ttctttaac agttcggtt gaggatttt acgattggg gccgaaagt tgcgacata cgacaagtg gggtattgt aatggagcg ctcaagagt gggtccact tatcgggtg aaaatacgg gcttttaca gcgcctgat aagttcact gatacgggg tacagtttt cccattcag acagatcaa gacaacacg aatataata ctcggagtt ttgattcca ctgatcctc ttcggcatc ctcttcttg gttatcatc tacataaga cggagaaga tcagctcgg aaggccacc gacacgcgg acaaacgac aacatgtcg cttccggat tcggtaatt gagaccagt cgaccggtc cgaatcgac agcttcgcc aaccactac cgactgatg tcggccgat tcggacttt cggttcagt gaagagttc gaagaactc aagcatgtg gggagagac caaccggcg acttttgcc gacctgccc tgcaacagg ccgaagaac cgcttcaca aacatcctg ccctacgac cactcaagg ttcaaactc cagccagtg gacgacgaa gaagggtcc gactacatc aacgccaac tatgtgccg ggctataac tccccgcgc gagtttata gtgacccaa gggccgtta cattcaacc cgtgacgac ttctggcgg atggtttgg gagtcgaac tcaagggct attatcatg ttgacacgg tgtgtggaa aaagggcgc gaaaagtgc gaccattat tggccctac gagtccatg cctgtgtac tatggggac atttcggtg caaattttg aacgaaact cggtatcct gactggaac atttccgag ttcatggtg tgtcggggt gaccaacag agggtgatc cgccacttc cacttcacc acgtggcct gacttcggt gtcccgaac ccccctcac accctcgta cgtttcgtc cgtgcgttt cgggagaga gtgggttcg gatcaacgc ccgattgtt gtccactgc agtgccgga gtgggccgt tccggcacg ttcatttgc ctggaccgc atcctgcag cagatcctc gtttcggac tacgtggac atcttcggt atcgtgtac gcaatgcgt aaggagcgt gtctggatg gtacagacc gaacagcag tacatttgc atccaccag tgccttttg gccgtgctg gagggcaaa gaactgtcc ggatcaccg cgtgaaatt cacgaaaat caaggattt gaagatgac gaaggcatt gcagagtct gggatgtga taaaaacga agatcggca agaagcaat aaagtgttt tatagaaac tagtagact tttgttaca tatatttta ttatgtaat cgcccatag atttgatat tatattaac tattgtaca gtatttcca caattcttt acagtcaga agcgacgca gtatttgtg acaagtctg gcaaatcat tccagatga gagcgccat aacacttca taatcaccg tactttctt cataaaatt attagtaat agaaaaaat accgttctg tgataaaac gattcggtt ttagtgtaa ttttatata aaccagcca cttggcgta aaataaccc gctttgtac ttaaactca aataaatat tttatgcaa cg
- >TC007672 RB
ttgcgtttg ttgtcacgc tggctcgca ggtgctgct ctctactcc ttggcgagg tcctctttg aaagtttcc tcatttcgt ttcgggtta ttgtcaaat gcaataatg gcgtagaag agcgtaacc gtgagtgcg tgtcgtgta atgccatca cggatgcat ctcaattga tacagagcc cccataccg gacataatt atgcctcgt atcccgcgc atcgccccc atttgaatc caccgtacc tgtctcttc tctacccta tattgttat attaaatta tttgtgtgt gtgtatgtg tatgttttt tatttgttg ttgttgttt gttgttgtt attgccatg accagtgtc gtttttttg cttggtgca gctttattc gggtcagtc aagttggtg caaacggcg gaacttgtc atccacatt ccgggcaat ttgggccaa gaaggggtg atttaccgc ctggactac tacccccct ttcggccac cccgccccc aacaccacc atcgcctcc aaagacatc caggacgtg atcaagttc tcgcaagcc ttaccaggc accaagtac gacttctgg ctctattac tccaactcc acgcacaat acgctcacc tggactgcg aattttaca accgtcccg gacccgccc tcgaatctc tcggttagc gtccgcagt ggtaaatct gcggtgatt tcgtggagt ccgccaaca caaggcagc tactccagc ttcaaactc aaaatcaac tgtctgaat aacgtacca gaggccaaa ccccaaact ctgacaatt gaccatgtt gataacaac tcctacctc ctcaaggag ctcacaccg ggggccacc tatcaagtg caagcgttc acggtgttt gaaaataag gaaagtgca gcctacacg tcacgaaat tttactaca aaaccgaac actccgggg aaatttatc gtgtggttc cggaacgag acaactttg cttgtttta tggcagccc ccttatcct gccgctatt tacacgcat tataaagtc tcaattgac cctcctgat gctaatgac agtatactg tacgtggag aaagagggt gagcctcca gggccggct caggcggcc tttaagggc ttggtgcca ggccgggct tacaatatt tcggtacaa acgatgtca gaagatgag atttcggca cctaccact gctcaatac cgaactgtg cctttaagg ccctccaaa ataaccttt agactcgtt acttcgtat tcgtttgaa gtccactgg gaggaaccc aatggtaaa agtgagttc gataaatac cacgttttt gtgggaacg acgaaaagt aagcaacag atcacgaaa atgcgcgac gatcccacg ttttacgag ttcagcgaa aacctagac cctggaaaa acctacagt gtgactgtt aaaacagtc tctgggaaa gtctcctcc tggcctgct acggccgaa gtgacttta aagccgctt cctgtgatg catgtgcat gccgaaagt gatattaaa aacggcatt gttaacata tcatggagt ccgaacccg cacagcttc caggacaac tacttggtg tcgtaccac gaagtcgag agttcgacc ggtgatagt aacaatata aagacaaat aacaagacc aaaataacg ctcgatacg cttctgcct ggtcggaat tattcactc attgtccaa gcgctctct aaagatatg gagtcgaat gagagttat ctctatgtg gttacacaa ccaagcgcg cccataatt gaagacctc agacccatg gtcgatggt ctcaacatc agctggaag agcgatgtc aattcacgc caagacaaa tacgaatta cactgtgtc cggaacgac accaatgat aaaattata aggacaacc catgactcc aggataatc ctctcgaat ctttacccg ggagctggc tatctgatc aaagttttc gccatttcg cacggcctg aagagcgaa ccccacgag tatttccag ccagttttc ccccgtccg cccaagaac atgacaatc gagaaaacc acgaccact tcggtcctg gtccagtgg ctggccccc accgattcg ctcatttcc gaatacgct attcgctac cgcaccgac accaataga aattgggtg cgattacca gcagtccag aatacagaa gccgaagtt accgacatg actcccggc gaacggtac acgattcaa gtgaataca gttagttac ggtttggaa agtaacgaa ccacaacaa atgaatcac acagttcga ccaaatcca gcatcaaac attgcacgt ttggttgat tcgaataat gtgactctg gaatggcca cgtcctgaa ggaagagtc gaaacgtac gttgttcga tggtgggaa tccgggaac gaatcggaa atctactcc cggaatgtg agtcagagc gagaatgga actgggcct gtgagacta ctggtcagt gatttaatg ccaggggtt gagtacacg tttgatatc aaagcgatt tcgaatgga ttggaaagt gacaagacg gtgttaaca atcaggaca atgcctttg atccaatca gaagtcgtg gtggtgacc aatcaacaa tcgacagac actataagt ttgaggtat acaccgact ccttcagct tcgtccaag ttcgatttt taccgattt tcactgtcg gatactaat attcctgac caggaaaaa gcctggaat gacacggac cgatttgtt actttcacc ggattagtg ccaggccga ctgtacaat attacagta tggacggtc agtcaaggg gtcgaaagt cagcccttg cagcgccag gatagactt tacccagaa cccatcacc ggcatcaac gcgacctcc atctccgac acggaaata accctctcc tgggcgtcc ccacgtggc gaattcaac gcctttgaa gtccaatac ctcaccttc gaaaacacc ctcatccac aacctcacc ctccagaac accatcacc atcaccgag ctcaaaccc caccgcaac tacacgttc acagtgatg gtgcgaagc ggcaccgag tcgagcatt ttgcgccaa tcaagccca gtttcgggc attttcacg accaaggaa tcggtccca ggccgagtg gaacgtttc gaacccttc gatatccaa cccagtagc atttccttc gagtggtcg ctccctcag accgagcag aacggcgtc atccgcaag ttcaccata acttacgga ctggaaggc tcctccaac acacaatca ctcgacttc aagccgaac gaattcaag ggaaaaatc cggcagtta ttacccggg aaaacctac gttttccgc atccaaggg gagacgaaa gtcgggttt ggacctgag agagtctgg aagcagaaa atgccgatc cgggaaccc cccgttccg aattcgagg gcggtcccg accgaagtg cgccgaagc tccacgacg attcaaatt cggttcagg aagaattat ttcagtgat gtgaacggg cctgtgacc acatatgcc attattgtg gcggaggat tattcgaaa aatgcctct gggttggaa atgcccagc tggagggac gttcaggcg tattctgtg tggccgccg tatcaagtt tcggagccg tattttccg ttctttaac agttcggtt gaggatttt acgattggg gccgaaagt tgcgacata cgacaagtg gggtattgt aatggagcg ctcaagagt gggtccact tatcgggtg aaaatacgg gcttttaca gcgcctgat aagttcact gatacgggg tacagtttt cccattcag acagatcaa gacaacacg aatataata ctcggagtt ttgattcca ctgatcctc ttcggcatc ctcttcttg gttatcatc tacataaga cggagaaga tcagctcgg aaggccacc gacacgcgg acaaacgac aacatgtcg cttccggat tcggtaatt gagaccagt cgaccggtc cgaatcgac agcttcgcc aaccactac cgactgatg tcggccgat tcggacttt cggttcagt gaagagttc gaagaactc aagcatgtg gggagagac caaccggcg acttttgcc gacctgccc tgcaacagg ccgaagaac cgcttcaca aacatcctg ccctacgac cactcaagg ttcaaactc cagccagtg gacgacgaa gaagggtcc gactacatc aacgccaac tatgtgccg ggctataac tccccgcgc gagtttata gtgacccaa gggccgtta cattcaacc cgtgacgac ttctggcgg atggtttgg gagtcgaac tcaagggct attatcatg ttgacacgg tgtgtggaa aaagggcgc gaaaagtgc gaccattat tggccctac gagtccatg cctgtgtac tatggggac atttcggtg caaattttg aacgaaact cggtatcct gactggaac atttccgag ttcatggtg tgtcggggt gaccaacag agggtgatc cgccacttc cacttcacc acgtggcct gacttcggt gtcccgaac ccccctcac accctcgta cgtttcgtc cgtgcgttt cgggagaga gtgggttcg gatcaacgc ccgattgtt gtccactgc agtgccgga gtgggccgt tccggcacg ttcatttgc ctggaccgc atcctgcag cagatcctc gtttcggac tacgtggac atcttcggt atcgtgtac gcaatgcgt aaggagcgt gtctggatg gtacagacc gaacagcag tacatttgc atccaccag tgccttttg gccgtgctg gagggcaaa gaactgtcc ggatcaccg cgtgaaatt cacgaaaat caaggattt gaaggtgag ttggtggag ggttttgta aatttccgc aatctcgaa ctgtaaatg cgattttgg tgtgtatgc agaaatggt aacacactt gtttagtcg atgtaaaaa gctgctatt ctgtggttc aggcaaagg tgttggtgg aaacggcgc tactgttgg gccgtcttt aggcacagg ggagaaaca aaggtagat taaccgtac acctggctg cacttaacg ctttagccg gtaacacat tttttacta agcatgggc ctgcacgac tcgtagatt agtgccta
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