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Gene information
General information
TC007824 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 1047957..1050791
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007824
    iBeetle screen iB_02752
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007824 RA
      MSFSKAKIQ RFNDVKECT PSPATYNVS LQEKVKLGL 
      ISKPGTYSK LGSPTRSET GSVHSTPCF QTPTLPLKK 
      KKFLSFKSA KTSSQQEEL REKIVECSN KDQYIRDLI 
      EQIEEMTGK VNNLERENL RLLEEKNGC EGALSDLRQ 
      QHLEDNLKR TKKYEGINT NLQNVNKQL RQVRREYEE 
      LKSQNLVEL SKLLSNIAK FQKLYDDSV KQSREETEA 
      LVKKLEDSE AKVVLLEDE LRGKNKEHS KKVETLLAQ 
      HETLLKKFE ENQIKAARL EGEFSQKLQ EKNEEVEAL 
      LKKLEDNEN ELREKNEEH SKKVETLLA QHDTLLKKL 
      EESQIKAAR LEEEFSQKL QEKNQELEE SEAKIALLE 
      RKNSEEHSK ETRVLLKKL EESQAKVTL LEENFKDVK 
      LLTDLQICD KIKEVEDGW KQKLEEKQR EHEAILKEC 
      QAISEYTII QSEVEKNQV KKLLEEKEQ SHQALVKKF 
      ELLEEEVGF MKSEKHKYQ LSISTFKDT IEVLKKRLF 
      NSDRDVEQL KEELEKCEE KILTYEKKI AELSSQLLE 
      TQTANEELE MQYESTSKL MQNQVQTIE KNLLSKVEV 
      LQNEAKQLK KDKQLLNEV LSQHHDQQK LLNEAQKAI 
      EKSHSLIYD LENRQSELE DRVRSYKLK LDEETEEAA 
      EIRKKYIEK SGKYDELAH QFEQLLQEL DKAKDRIQE 
      LENLIGPYQ EQLEAYQNE LVVLTNEKN VFENEAKEL 
      SLKYAEILG HQNHSQKIK HIEDLKTKY LEKTKKNVD 
      LEAKNLKLN KLIDKLSRQ IEDMNKPGK KYKLVEDKE 
      NLASPKSLK GTQSPGPLK DKN
    CDS sequence
    • >TC007824 RA
      atgtcgttc tccaaagcc aaaatccaa agatttaat 
      gacgttaaa gagtgtact ccatcaccg gcaacatac 
      aatgtttcc ttgcaagaa aaagtcaaa ctcggtcta 
      atttcaaaa ccaggaacg tactcaaaa cttgggagc 
      cccacgcgg tcagagacg ggctcagtt cacagcaca 
      ccttgtttt caaacacct actttgccc ttgaagaaa 
      aagaagttt ttatcgttc aaaagcgcg aaaaccagc 
      agtcaacaa gaagaactg agggaaaaa atagtcgaa 
      tgtagtaat aaagatcaa tacatcagg gatttaatc 
      gaacaaatt gaagaaatg accgggaaa gttaataat 
      ctggaacgg gagaatcta cgtttactt gaggaaaaa 
      aacgggtgt gagggagcc ttatctgat ttgaggcaa 
      caacacctt gaagataat cttaaacgt actaaaaaa 
      tacgagggg ataaacacc aatcttcaa aatgtaaac 
      aaacagctg cgtcaagtt cgccgagaa tatgaagaa 
      ctgaagtcg caaaatttg gtcgagttg agcaagttg 
      ttgagcaat attgccaag tttcagaag ttgtacgac 
      gacagtgtg aagcaaagt cgcgaagaa actgaagca 
      ctggtgaag aaactcgaa gatagcgaa gccaaagtt 
      gtgctactt gaagatgaa ttgaggggg aagaataaa 
      gaacacagc aaaaaagtc gaaacgttg ctagctcag 
      catgaaaca ctattgaag aaatttgaa gaaaaccag 
      attaaagcc gcacggctt gaaggggaa ttttcacag 
      aagctacaa gagaagaat gaagaagtc gaagcactg 
      ttgaagaaa ctcgaagat aatgaaaat gagttgcgg 
      gaaaaaaat gaagaacac agcaaaaaa gtcgaaacg 
      ttgctagct caacatgac acactattg aagaaactt 
      gaagaaagc cagattaaa gctgcacgt cttgaagag 
      gaattttca cagaagtta caagagaag aatcaagaa 
      cttgaagaa agtgaagct aaaatcgca ctgcttgaa 
      cggaagaac agtgaagaa catagcaag gagacacgt 
      gttctcttg aagaaactt gaagaaagc caagctaag 
      gtcaccctt cttgaagag aacttcaaa gatgtgaaa 
      cttctgact gatcttcaa atatgcgac aagattaaa 
      gaagttgaa gacggatgg aagcaaaaa cttgaagaa 
      aaacaaaga gaacatgaa gcaattcta aaagagtgc 
      caagcaatt agtgagtac accatcatt caaagcgaa 
      gtggagaag aaccaagtg aagaaattg cttgaagag 
      aaagaacaa tcccaccag gccttggtg aagaaattt 
      gagctgttg gaagaagaa gttggcttt atgaagagc 
      gagaaacac aaataccaa ctttcaatc agcacattc 
      aaggacact attgaagtt ttgaagaaa cgtttgttc 
      aattcggac cgtgatgtt gaacaattg aaggaagaa 
      ctggaaaaa tgtgaagaa aaaatccta acgtatgag 
      aaaaaaatt gcggaactg tcgagtcaa ttgctcgaa 
      actcaaacc gcaaatgaa gaactggaa atgcagtac 
      gaatccacc tctaaactt atgcaaaat caggtccaa 
      accatcgaa aaaaaccta ttatccaaa gttgaagtt 
      cttcaaaac gaggccaaa caactgaag aaggacaag 
      caattgtta aacgaggtt ttatcgcaa caccacgac 
      caacaaaag ctactaaac gaagcacaa aaagctatc 
      gaaaaatcg cactcactt atctacgat ttggaaaac 
      agacaatca gagttggaa gatagagtt cgttcgtat 
      aaattaaaa ctggatgaa gaaacggaa gaagccgcc 
      gaaatcagg aagaaatac atcgagaag agtgggaaa 
      tacgacgag ttggcccac caattcgag caactcctt 
      caggagctg gacaaggcc aaggaccgc atccaggag 
      ctggagaat ttgattggc ccgtatcag gagcaactg 
      gaagcgtac caaaacgaa ctcgtcgtg cttacaaac 
      gagaaaaac gtctttgag aatgaggcc aaagagttg 
      agtttgaag tacgccgaa attttagga caccagaac 
      cacagtcag aagatcaaa catatcgaa gatttgaag 
      accaaatat ttggaaaag acgaagaag aatgtggat 
      ctcgaagcg aaaaacttg aagctgaat aagttgatt 
      gataagttg tcaaggcaa attgaagat atgaataaa 
      ccagggaag aagtataaa ctcgttgag gataaggag 
      aatttggct agtcccaag agtttgaaa gggacgcaa 
      agtccggga cccctcaag gataaaaat tag
    mRNA sequence
    • >TC007824 RA
      cgtcacagc cgttacgat agtttaatc atttttcag 
      ctggtcgtg ctcgcgttt tcaatgtta ttttttata 
      gtttatttg ttatcttag tgtttttat tgttaaaat 
      gtcgttctc caaagccaa aatccaaag atttaatga 
      cgttaaaga gtgtactcc atcaccggc aacatacaa 
      tgtttcctt gcaagaaaa agtcaaact cggtctaat 
      ttcaaaacc aggaacgta ctcaaaact tgggagccc 
      cacgcggtc agagacggg ctcagttca cagcacacc 
      ttgttttca aacacctac tttgccctt gaagaaaaa 
      gaagttttt atcgttcaa aagcgcgaa aaccagcag 
      tcaacaaga agaactgag ggaaaaaat agtcgaatg 
      tagtaataa agatcaata catcaggga tttaatcga 
      acaaattga agaaatgac cgggaaagt taataatct 
      ggaacggga gaatctacg tttacttga ggaaaaaaa 
      cgggtgtga gggagcctt atctgattt gaggcaaca 
      acaccttga agataatct taaacgtac taaaaaata 
      cgaggggat aaacaccaa tcttcaaaa tgtaaacaa 
      acagctgcg tcaagttcg ccgagaata tgaagaact 
      gaagtcgca aaatttggt cgagttgag caagttgtt 
      gagcaatat tgccaagtt tcagaagtt gtacgacga 
      cagtgtgaa gcaaagtcg cgaagaaac tgaagcact 
      ggtgaagaa actcgaaga tagcgaagc caaagttgt 
      gctacttga agatgaatt gagggggaa gaataaaga 
      acacagcaa aaaagtcga aacgttgct agctcagca 
      tgaaacact attgaagaa atttgaaga aaaccagat 
      taaagccgc acggcttga aggggaatt ttcacagaa 
      gctacaaga gaagaatga agaagtcga agcactgtt 
      gaagaaact cgaagataa tgaaaatga gttgcggga 
      aaaaaatga agaacacag caaaaaagt cgaaacgtt 
      gctagctca acatgacac actattgaa gaaacttga 
      agaaagcca gattaaagc tgcacgtct tgaagagga 
      attttcaca gaagttaca agagaagaa tcaagaact 
      tgaagaaag tgaagctaa aatcgcact gcttgaacg 
      gaagaacag tgaagaaca tagcaagga gacacgtgt 
      tctcttgaa gaaacttga agaaagcca agctaaggt 
      cacccttct tgaagagaa cttcaaaga tgtgaaact 
      tctgactga tcttcaaat atgcgacaa gattaaaga 
      agttgaaga cggatggaa gcaaaaact tgaagaaaa 
      acaaagaga acatgaagc aattctaaa agagtgcca 
      agcaattag tgagtacac catcattca aagcgaagt 
      ggagaagaa ccaagtgaa gaaattgct tgaagagaa 
      agaacaatc ccaccaggc cttggtgaa gaaatttga 
      gctgttgga agaagaagt tggctttat gaagagcga 
      gaaacacaa ataccaact ttcaatcag cacattcaa 
      ggacactat tgaagtttt gaagaaacg tttgttcaa 
      ttcggaccg tgatgttga acaattgaa ggaagaact 
      ggaaaaatg tgaagaaaa aatcctaac gtatgagaa 
      aaaaattgc ggaactgtc gagtcaatt gctcgaaac 
      tcaaaccgc aaatgaaga actggaaat gcagtacga 
      atccacctc taaacttat gcaaaatca ggtccaaac 
      catcgaaaa aaacctatt atccaaagt tgaagttct 
      tcaaaacga ggccaaaca actgaagaa ggacaagca 
      attgttaaa cgaggtttt atcgcaaca ccacgacca 
      acaaaagct actaaacga agcacaaaa agctatcga 
      aaaatcgca ctcacttat ctacgattt ggaaaacag 
      acaatcaga gttggaaga tagagttcg ttcgtataa 
      attaaaact ggatgaaga aacggaaga agccgccga 
      aatcaggaa gaaatacat cgagaagag tgggaaata 
      cgacgagtt ggcccacca attcgagca actccttca 
      ggagctgga caaggccaa ggaccgcat ccaggagct 
      ggagaattt gattggccc gtatcagga gcaactgga 
      agcgtacca aaacgaact cgtcgtgct tacaaacga 
      gaaaaacgt ctttgagaa tgaggccaa agagttgag 
      tttgaagta cgccgaaat tttaggaca ccagaacca 
      cagtcagaa gatcaaaca tatcgaaga tttgaagac 
      caaatattt ggaaaagac gaagaagaa tgtggatct 
      cgaagcgaa aaacttgaa gctgaataa gttgattga 
      taagttgtc aaggcaaat tgaagatat gaataaacc 
      agggaagaa gtataaact cgttgagga taaggagaa 
      tttggctag tcccaagag tttgaaagg gacgcaaag 
      tccgggacc cctcaagga taaaaatta ggataaggc 
      tcacttttt taatttatt atttgattg tgttctaaa 
      aaaggctga ttagtgtta aaagaattg taggtttta 
      tacaagtaa ctccgttta gtcatagct gcgattcgt 
      ggaatccaa aggttttat gtcaatgaa gtacaaaat 
      atatttgat gcacttttt attaaaccg ttttattta 
      tc