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Gene information
General information
TC007822 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1006983..1028178
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.

    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.

    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02751

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Left: Right:
    Protein sequence
    • >TC007822 RA
    CDS sequence
    • >TC007822 RA
      atgaatcgg tctgaacag gaaagcttg taccgcacg 
      tcccgcaaa ctgagcttg gtgccgatc ggcagctcg 
      ccgcacgcg ggcgccccc tccgacgac ctggtgctg 
      agcgtgctg aagagtccc gtgaaccac gtggcgcgc 
      gatgaggag gcgccccag gtgcccagc gaggaggac 
      gagtgctcc gagcgcctg cccttcctg cagcgcctg 
      cggggccac cggccgcgc ttcggcgaa gcgcaccac 
      aagcgcgtc ccgacgccg ctcaagcgc aagcagcgc 
      ttccgcagc cacaagaac gacgtggtg cagcccgag 
      gacgtcgtc gacgaggag gaggacgtg gcggcgccg 
      acggacgag cccaggggc gtccccgac ccctactat 
      cccatttat ttgcccatc gatcaggcg ttcaaggcc 
      aagtatgtt ttccaccac aagaagggg aagacgtgc 
      caggagagg acgtacgtt tttctggag catcccgga 
      ggatggctg tgctttatc tatcacttt actgtgttt 
      atgttagtc ctcgtttgc ctaatattc agcgtgttg 
      tcaacgatc gacacgtac caaagcttt gccaacgag 
      acccttttc tggatggag atttgcctg gtggttttc 
      ttcggcgtt gagtatttg gtgcggctg tggtcggca 
      ggctgcagg tcaaagtac atgggcttt tgggggcgc 
      cttcgattt atacgcaaa cccatctgc attatcgat 
      ttaattgta gtggtagct tcgattgta gttctaacg 
      ttaggttca aacgggcaa gtgtttgcc acgtcggct 
      atccgcgga attcgcttc ctgcagatt ttgcggatg 
      ctccacgtg gacaggcaa gggggcacg tggaggctg 
      ctgggttca gttgtgttc atacatcga caagaatta 
      ataacgact ctttacatc ggatttctg ggcctaatt 
      ttcagcagt tatttcgtt tatttggcg gagaaggac 
      gctgtgggt gctgatgga aaaaatacg ggggatttt 
      tcgagttat gcggatgcg ctctggtgg ggggttatc 
      actgttacg accattgga tatggggac actgtgccc 
      aggacttgg atgggcaag attgtagcg tcgtgtttt 
      agcgtcttt gccatatct ttcttcgct cttccagcc 
      ggcatcctc ggctcgggc ttcgccctc aaggtgcag 
      cagaagcaa cgccagaag catttcaat cggcagatt 
      cctgccgcc gccatgctc atccagtgt ttgtggcgg 
      tgttacgcc gccgacaaa agtttcaat tcgagggca 
      acgtggcag atttatctc aaggatacg aacggcttc 
      accaccaac aactcttcc agtgggagc ggcaccaat 
      tgtaacata cctctttcc aaggtggct aagaaagcg 
      tccgttttg aaacgtcgg aaatcgcgg aatcgcatg 
      gaggcgcct ggaacgcca cagggcgac acccccgtc 
      caagcagtc gcccccaat gcaccctcc aggtgcgag 
      tccgattcc gacgttgtt ttctacatg gaggagcct 
      aaagccggc accccaaac cgcgtccgc cgaggcgaa 
      cgtgaaagc tcccgtgga gccgtcttt accagccag 
      accagcacc gtcacggag gccgccagc gacgacatc 
      gacgacctc gacggcgag ctcccccgg gtgacacaa 
      ctcacagaa gcccacaag aacgccatc cgagcgata 
      cgaaaaatc aaatatttc gtcgccaga cgcaagttc 
      cagcaggcg cgcaagccc tacgacgtg cgggacgtc 
      atcgagcag tacagccaa ggccacctc aacatgatg 
      gtccgcatc aaggagtta cagcgacgg ctggaccag 
      acgctgggc aaacctggg agttaccta gcggggatc 
      gatcgcgtt ggaaacgtc aaacccatg acggtgggt 
      gcgagactg taccgagtg gaacagcag ctggggacc 
      atggacaag aagctggac gccttaact cacattctg 
      aactcgttg gcacaaaaa aatcaagct gcgccactt 
      aggtcgatc gaagatgat gtctga
    mRNA sequence
    • >TC007822 RA
      gtcgttgaa taatccagt gcacattgt tgtgccaag 
      gatttttat ccggcaatt agcgtctaa agttgtcac 
      cccctggct tcctggcgc ttaaagtcg acctacctg 
      atttgattc cgccgtggc ttagatcga tgtgagcgt 
      cgtcggcgt cgtcatcgt gtgagcgtc cctgcgcga 
      gaccaacag ttggttttc ggacgcgtt ggaagccgc 
      caccgccac cgccgccgc cgccgccgc caccgttgc 
      attattcag cgcgaccat tacgagttg tttcgtcga 
      taaatcggg ggctctgcc ccccgcgcc gccctaatt 
      tggcggagg cgccgggat aagggggcc cgactcgcg 
      gtatgacac gggttacgg atgtggggc ttttgccgc 
      ccaatttcg aactgtggg cttttggtt gaggtgatc 
      acgcacccc ctccgacga tgccgtcgt gatgcctcc 
      gttcgtgtc ggcccggcc cccgttcgc aaatgctgt 
      cattagtga tgttaagcg agatgaatc ggtctgaac 
      aggaaagct tgtaccgca cgtcccgca aactgagct 
      tggtgccga tcggcagct cgccgcacg cgggcgccc 
      cctccgacg acctggtgc tgagcgtgc tgaagagtc 
      ccgtgaacc acgtggcgc gcgatgagg aggcgcccc 
      aggtgccca gcgaggagg acgagtgct ccgagcgcc 
      tgcccttcc tgcagcgcc tgcggggcc accggccgc 
      gcttcggcg aagcgcacc acaagcgcg tcccgacgc 
      cgctcaagc gcaagcagc gcttccgca gccacaaga 
      acgacgtgg tgcagcccg aggacgtcg tcgacgagg 
      aggaggacg tggcggcgc cgacggacg agcccaggg 
      gcgtccccg acccctact atcccattt atttgccca 
      tcgatcagg cgttcaagg ccaagtatg ttttccacc 
      acaagaagg ggaagacgt gccaggaga ggacgtacg 
      tttttctgg agcatcccg gaggatggc tgtgcttta 
      tctatcact ttactgtgt ttatgttag tcctcgttt 
      gcctaatat tcagcgtgt tgtcaacga tcgacacgt 
      accaaagct ttgccaacg agacccttt tctggatgg 
      agatttgcc tggtggttt tcttcggcg ttgagtatt 
      tggtgcggc tgtggtcgg caggctgca ggtcaaagt 
      acatgggct tttgggggc gccttcgat ttatacgca 
      aacccatct gcattatcg atttaattg tagtggtag 
      cttcgattg tagttctaa cgttaggtt caaacgggc 
      aagtgtttg ccacgtcgg ctatccgcg gaattcgct 
      tcctgcaga ttttgcgga tgctccacg tggacaggc 
      aagggggca cgtggaggc tgctgggtt cagttgtgt 
      tcatacatc gacaagaat taataacga ctctttaca 
      tcggatttc tgggcctaa ttttcagca gttatttcg 
      tttatttgg cggagaagg acgctgtgg gtgctgatg 
      gaaaaaata cgggggatt tttcgagtt atgcggatg 
      cgctctggt ggggggtta tcactgtta cgaccattg 
      gatatgggg acactgtgc ccaggactt ggatgggca 
      agattgtag cgtcgtgtt ttagcgtct ttgccatat 
      ctttcttcg ctcttccag ccggcatcc tcggctcgg 
      gcttcgccc tcaaggtgc agcagaagc aacgccaga 
      agcatttca atcggcaga ttcctgccg ccgccatgc 
      tcatccagt gtttgtggc ggtgttacg ccgccgaca 
      aaagtttca attcgaggg caacgtggc agatttatc 
      tcaaggata cgaacggct tcaccacca acaactctt 
      ccagtggga gcggcacca attgtaaca tacctcttt 
      ccaaggtgg ctaagaaag cgtccgttt tgaaacgtc 
      ggaaatcgc ggaatcgca tggaggcgc ctggaacgc 
      cacagggcg acacccccg tccaagcag tcgccccca 
      atgcaccct ccaggtgcg agtccgatt ccgacgttg 
      ttttctaca tggaggagc ctaaagccg gcaccccaa 
      accgcgtcc gccgaggcg aacgtgaaa gctcccgtg 
      gagccgtct ttaccagcc agaccagca ccgtcacgg 
      aggccgcca gcgacgaca tcgacgacc tcgacggcg 
      agctccccc gggtgacac aactcacag aagcccaca 
      agaacgcca tccgagcga tacgaaaaa tcaaatatt 
      tcgtcgcca gacgcaagt tccagcagg cgcgcaagc 
      cctacgacg tgcgggacg tcatcgagc agtacagcc 
      aaggccacc tcaacatga tggtccgca tcaaggagt 
      tacagcgac ggctggacc agacgctgg gcaaacctg 
      ggagttacc tagcgggga tcgatcgcg ttggaaacg 
      tcaaaccca tgacggtgg gtgcgagac tgtaccgag 
      tggaacagc agctgggga ccatggaca agaagctgg 
      acgccttaa ctcacattc tgaactcgt tggcacaaa 
      aaaatcaag ctgcgccac ttaggtcga tcgaagatg 
      atgtctgag caataatct tgcaactac atatccagt 
      gggattggc taacttact gtacatatg agtagttta 
      agggaattt agtcaatat tcaaaacta agcatggta 
      ttatggggc acacgtgta aattgatca aggatcaat 
      tgtaatata attatattt ttagccact tttagatta 
      tttccatat ctatatcta gaccgtgcc tatttacta 
      gatttatat gagaattaa aatagggag cgtacgagc 
      ggtctcgaa cacatgcag gcgtttttc ataacggaa 
      tcaaaattt catgttgca gacaatcgt tacgaggta 
      agttaggga taagatttg atgcaagac aaggtaggt 
      ttggtttca catgaaatt attataaaa caacctctc 
      tgatagagc tcttttctc aaaaaaaat aaataaaat 
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY