| General information | |||
| TC007822 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 1006983..1028178 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007822 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02751 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC007822 RA
MNRSEQESL YRTSRKLSL VPIGSSPHA GAPSDDLVL SVLKSPVNH VARDEEAPQ VPSEEDECS ERLPFLQRL RGHRPRFGE AHHKRVPTP LKRKQRFRS HKNDVVQPE DVVDEEEDV AAPTDEPRG VPDPYYPIY LPIDQAFKA KYVFHHKKG KTCQERTYV FLEHPGGWL CFIYHFTVF MLVLVCLIF SVLSTIDTY QSFANETLF WMEICLVVF FGVEYLVRL WSAGCRSKY MGFWGRLRF IRKPICIID LIVVVASIV VLTLGSNGQ VFATSAIRG IRFLQILRM LHVDRQGGT WRLLGSVVF IHRQELITT LYIGFLGLI FSSYFVYLA EKDAVGADG KNTGDFSSY ADALWWGVI TVTTIGYGD TVPRTWMGK IVASCFSVF AISFFALPA GILGSGFAL KVQQKQRQK HFNRQIPAA AMLIQCLWR CYAADKSFN SRATWQIYL KDTNGFTTN NSSSGSGTN CNIPLSKVA KKASVLKRR KSRNRMEAP GTPQGDTPV QAVAPNAPS RCESDSDVV FYMEEPKAG TPNRVRRGE RESSRGAVF TSQTSTVTE AASDDIDDL DGELPRVTQ LTEAHKNAI RAIRKIKYF VARRKFQQA RKPYDVRDV IEQYSQGHL NMMVRIKEL QRRLDQTLG KPGSYLAGI DRVGNVKPM TVGARLYRV EQQLGTMDK KLDALTHIL NSLAQKNQA APLRSIEDD V
- >TC007822 RA
atgaatcgg tctgaacag gaaagcttg taccgcacg tcccgcaaa ctgagcttg gtgccgatc ggcagctcg ccgcacgcg ggcgccccc tccgacgac ctggtgctg agcgtgctg aagagtccc gtgaaccac gtggcgcgc gatgaggag gcgccccag gtgcccagc gaggaggac gagtgctcc gagcgcctg cccttcctg cagcgcctg cggggccac cggccgcgc ttcggcgaa gcgcaccac aagcgcgtc ccgacgccg ctcaagcgc aagcagcgc ttccgcagc cacaagaac gacgtggtg cagcccgag gacgtcgtc gacgaggag gaggacgtg gcggcgccg acggacgag cccaggggc gtccccgac ccctactat cccatttat ttgcccatc gatcaggcg ttcaaggcc aagtatgtt ttccaccac aagaagggg aagacgtgc caggagagg acgtacgtt tttctggag catcccgga ggatggctg tgctttatc tatcacttt actgtgttt atgttagtc ctcgtttgc ctaatattc agcgtgttg tcaacgatc gacacgtac caaagcttt gccaacgag acccttttc tggatggag atttgcctg gtggttttc ttcggcgtt gagtatttg gtgcggctg tggtcggca ggctgcagg tcaaagtac atgggcttt tgggggcgc cttcgattt atacgcaaa cccatctgc attatcgat ttaattgta gtggtagct tcgattgta gttctaacg ttaggttca aacgggcaa gtgtttgcc acgtcggct atccgcgga attcgcttc ctgcagatt ttgcggatg ctccacgtg gacaggcaa gggggcacg tggaggctg ctgggttca gttgtgttc atacatcga caagaatta ataacgact ctttacatc ggatttctg ggcctaatt ttcagcagt tatttcgtt tatttggcg gagaaggac gctgtgggt gctgatgga aaaaatacg ggggatttt tcgagttat gcggatgcg ctctggtgg ggggttatc actgttacg accattgga tatggggac actgtgccc aggacttgg atgggcaag attgtagcg tcgtgtttt agcgtcttt gccatatct ttcttcgct cttccagcc ggcatcctc ggctcgggc ttcgccctc aaggtgcag cagaagcaa cgccagaag catttcaat cggcagatt cctgccgcc gccatgctc atccagtgt ttgtggcgg tgttacgcc gccgacaaa agtttcaat tcgagggca acgtggcag atttatctc aaggatacg aacggcttc accaccaac aactcttcc agtgggagc ggcaccaat tgtaacata cctctttcc aaggtggct aagaaagcg tccgttttg aaacgtcgg aaatcgcgg aatcgcatg gaggcgcct ggaacgcca cagggcgac acccccgtc caagcagtc gcccccaat gcaccctcc aggtgcgag tccgattcc gacgttgtt ttctacatg gaggagcct aaagccggc accccaaac cgcgtccgc cgaggcgaa cgtgaaagc tcccgtgga gccgtcttt accagccag accagcacc gtcacggag gccgccagc gacgacatc gacgacctc gacggcgag ctcccccgg gtgacacaa ctcacagaa gcccacaag aacgccatc cgagcgata cgaaaaatc aaatatttc gtcgccaga cgcaagttc cagcaggcg cgcaagccc tacgacgtg cgggacgtc atcgagcag tacagccaa ggccacctc aacatgatg gtccgcatc aaggagtta cagcgacgg ctggaccag acgctgggc aaacctggg agttaccta gcggggatc gatcgcgtt ggaaacgtc aaacccatg acggtgggt gcgagactg taccgagtg gaacagcag ctggggacc atggacaag aagctggac gccttaact cacattctg aactcgttg gcacaaaaa aatcaagct gcgccactt aggtcgatc gaagatgat gtctga
- >TC007822 RA
gtcgttgaa taatccagt gcacattgt tgtgccaag gatttttat ccggcaatt agcgtctaa agttgtcac cccctggct tcctggcgc ttaaagtcg acctacctg atttgattc cgccgtggc ttagatcga tgtgagcgt cgtcggcgt cgtcatcgt gtgagcgtc cctgcgcga gaccaacag ttggttttc ggacgcgtt ggaagccgc caccgccac cgccgccgc cgccgccgc caccgttgc attattcag cgcgaccat tacgagttg tttcgtcga taaatcggg ggctctgcc ccccgcgcc gccctaatt tggcggagg cgccgggat aagggggcc cgactcgcg gtatgacac gggttacgg atgtggggc ttttgccgc ccaatttcg aactgtggg cttttggtt gaggtgatc acgcacccc ctccgacga tgccgtcgt gatgcctcc gttcgtgtc ggcccggcc cccgttcgc aaatgctgt cattagtga tgttaagcg agatgaatc ggtctgaac aggaaagct tgtaccgca cgtcccgca aactgagct tggtgccga tcggcagct cgccgcacg cgggcgccc cctccgacg acctggtgc tgagcgtgc tgaagagtc ccgtgaacc acgtggcgc gcgatgagg aggcgcccc aggtgccca gcgaggagg acgagtgct ccgagcgcc tgcccttcc tgcagcgcc tgcggggcc accggccgc gcttcggcg aagcgcacc acaagcgcg tcccgacgc cgctcaagc gcaagcagc gcttccgca gccacaaga acgacgtgg tgcagcccg aggacgtcg tcgacgagg aggaggacg tggcggcgc cgacggacg agcccaggg gcgtccccg acccctact atcccattt atttgccca tcgatcagg cgttcaagg ccaagtatg ttttccacc acaagaagg ggaagacgt gccaggaga ggacgtacg tttttctgg agcatcccg gaggatggc tgtgcttta tctatcact ttactgtgt ttatgttag tcctcgttt gcctaatat tcagcgtgt tgtcaacga tcgacacgt accaaagct ttgccaacg agacccttt tctggatgg agatttgcc tggtggttt tcttcggcg ttgagtatt tggtgcggc tgtggtcgg caggctgca ggtcaaagt acatgggct tttgggggc gccttcgat ttatacgca aacccatct gcattatcg atttaattg tagtggtag cttcgattg tagttctaa cgttaggtt caaacgggc aagtgtttg ccacgtcgg ctatccgcg gaattcgct tcctgcaga ttttgcgga tgctccacg tggacaggc aagggggca cgtggaggc tgctgggtt cagttgtgt tcatacatc gacaagaat taataacga ctctttaca tcggatttc tgggcctaa ttttcagca gttatttcg tttatttgg cggagaagg acgctgtgg gtgctgatg gaaaaaata cgggggatt tttcgagtt atgcggatg cgctctggt ggggggtta tcactgtta cgaccattg gatatgggg acactgtgc ccaggactt ggatgggca agattgtag cgtcgtgtt ttagcgtct ttgccatat ctttcttcg ctcttccag ccggcatcc tcggctcgg gcttcgccc tcaaggtgc agcagaagc aacgccaga agcatttca atcggcaga ttcctgccg ccgccatgc tcatccagt gtttgtggc ggtgttacg ccgccgaca aaagtttca attcgaggg caacgtggc agatttatc tcaaggata cgaacggct tcaccacca acaactctt ccagtggga gcggcacca attgtaaca tacctcttt ccaaggtgg ctaagaaag cgtccgttt tgaaacgtc ggaaatcgc ggaatcgca tggaggcgc ctggaacgc cacagggcg acacccccg tccaagcag tcgccccca atgcaccct ccaggtgcg agtccgatt ccgacgttg ttttctaca tggaggagc ctaaagccg gcaccccaa accgcgtcc gccgaggcg aacgtgaaa gctcccgtg gagccgtct ttaccagcc agaccagca ccgtcacgg aggccgcca gcgacgaca tcgacgacc tcgacggcg agctccccc gggtgacac aactcacag aagcccaca agaacgcca tccgagcga tacgaaaaa tcaaatatt tcgtcgcca gacgcaagt tccagcagg cgcgcaagc cctacgacg tgcgggacg tcatcgagc agtacagcc aaggccacc tcaacatga tggtccgca tcaaggagt tacagcgac ggctggacc agacgctgg gcaaacctg ggagttacc tagcgggga tcgatcgcg ttggaaacg tcaaaccca tgacggtgg gtgcgagac tgtaccgag tggaacagc agctgggga ccatggaca agaagctgg acgccttaa ctcacattc tgaactcgt tggcacaaa aaaatcaag ctgcgccac ttaggtcga tcgaagatg atgtctgag caataatct tgcaactac atatccagt gggattggc taacttact gtacatatg agtagttta agggaattt agtcaatat tcaaaacta agcatggta ttatggggc acacgtgta aattgatca aggatcaat tgtaatata attatattt ttagccact tttagatta tttccatat ctatatcta gaccgtgcc tatttacta gatttatat gagaattaa aatagggag cgtacgagc ggtctcgaa cacatgcag gcgtttttc ataacggaa tcaaaattt catgttgca gacaatcgt tacgaggta agttaggga taagatttg atgcaagac aaggtaggt ttggtttca catgaaatt attataaaa caacctctc tgatagagc tcttttctc aaaaaaaat aaataaaat aaacgaat
