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Gene information
General information
TC007822 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1006983..1028178
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC007822
    iBeetle screen iB_02751

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007822 RA
      MNRSEQESL YRTSRKLSL VPIGSSPHA GAPSDDLVL 
SVLKSPVNH VARDEEAPQ VPSEEDECS ERLPFLQRL 
RGHRPRFGE AHHKRVPTP LKRKQRFRS HKNDVVQPE 
DVVDEEEDV AAPTDEPRG VPDPYYPIY LPIDQAFKA 
KYVFHHKKG KTCQERTYV FLEHPGGWL CFIYHFTVF 
MLVLVCLIF SVLSTIDTY QSFANETLF WMEICLVVF 
FGVEYLVRL WSAGCRSKY MGFWGRLRF IRKPICIID 
LIVVVASIV VLTLGSNGQ VFATSAIRG IRFLQILRM 
LHVDRQGGT WRLLGSVVF IHRQELITT LYIGFLGLI 
FSSYFVYLA EKDAVGADG KNTGDFSSY ADALWWGVI 
TVTTIGYGD TVPRTWMGK IVASCFSVF AISFFALPA 
GILGSGFAL KVQQKQRQK HFNRQIPAA AMLIQCLWR 
CYAADKSFN SRATWQIYL KDTNGFTTN NSSSGSGTN 
CNIPLSKVA KKASVLKRR KSRNRMEAP GTPQGDTPV 
QAVAPNAPS RCESDSDVV FYMEEPKAG TPNRVRRGE 
RESSRGAVF TSQTSTVTE AASDDIDDL DGELPRVTQ 
LTEAHKNAI RAIRKIKYF VARRKFQQA RKPYDVRDV 
IEQYSQGHL NMMVRIKEL QRRLDQTLG KPGSYLAGI 
DRVGNVKPM TVGARLYRV EQQLGTMDK KLDALTHIL 
NSLAQKNQA APLRSIEDD V
    CDS sequence
    • >TC007822 RA
      atgaatcgg tctgaacag gaaagcttg taccgcacg 
tcccgcaaa ctgagcttg gtgccgatc ggcagctcg 
ccgcacgcg ggcgccccc tccgacgac ctggtgctg 
agcgtgctg aagagtccc gtgaaccac gtggcgcgc 
gatgaggag gcgccccag gtgcccagc gaggaggac 
gagtgctcc gagcgcctg cccttcctg cagcgcctg 
cggggccac cggccgcgc ttcggcgaa gcgcaccac 
aagcgcgtc ccgacgccg ctcaagcgc aagcagcgc 
ttccgcagc cacaagaac gacgtggtg cagcccgag 
gacgtcgtc gacgaggag gaggacgtg gcggcgccg 
acggacgag cccaggggc gtccccgac ccctactat 
cccatttat ttgcccatc gatcaggcg ttcaaggcc 
aagtatgtt ttccaccac aagaagggg aagacgtgc 
caggagagg acgtacgtt tttctggag catcccgga 
ggatggctg tgctttatc tatcacttt actgtgttt 
atgttagtc ctcgtttgc ctaatattc agcgtgttg 
tcaacgatc gacacgtac caaagcttt gccaacgag 
acccttttc tggatggag atttgcctg gtggttttc 
ttcggcgtt gagtatttg gtgcggctg tggtcggca 
ggctgcagg tcaaagtac atgggcttt tgggggcgc 
cttcgattt atacgcaaa cccatctgc attatcgat 
ttaattgta gtggtagct tcgattgta gttctaacg 
ttaggttca aacgggcaa gtgtttgcc acgtcggct 
atccgcgga attcgcttc ctgcagatt ttgcggatg 
ctccacgtg gacaggcaa gggggcacg tggaggctg 
ctgggttca gttgtgttc atacatcga caagaatta 
ataacgact ctttacatc ggatttctg ggcctaatt 
ttcagcagt tatttcgtt tatttggcg gagaaggac 
gctgtgggt gctgatgga aaaaatacg ggggatttt 
tcgagttat gcggatgcg ctctggtgg ggggttatc 
actgttacg accattgga tatggggac actgtgccc 
aggacttgg atgggcaag attgtagcg tcgtgtttt 
agcgtcttt gccatatct ttcttcgct cttccagcc 
ggcatcctc ggctcgggc ttcgccctc aaggtgcag 
cagaagcaa cgccagaag catttcaat cggcagatt 
cctgccgcc gccatgctc atccagtgt ttgtggcgg 
tgttacgcc gccgacaaa agtttcaat tcgagggca 
acgtggcag atttatctc aaggatacg aacggcttc 
accaccaac aactcttcc agtgggagc ggcaccaat 
tgtaacata cctctttcc aaggtggct aagaaagcg 
tccgttttg aaacgtcgg aaatcgcgg aatcgcatg 
gaggcgcct ggaacgcca cagggcgac acccccgtc 
caagcagtc gcccccaat gcaccctcc aggtgcgag 
tccgattcc gacgttgtt ttctacatg gaggagcct 
aaagccggc accccaaac cgcgtccgc cgaggcgaa 
cgtgaaagc tcccgtgga gccgtcttt accagccag 
accagcacc gtcacggag gccgccagc gacgacatc 
gacgacctc gacggcgag ctcccccgg gtgacacaa 
ctcacagaa gcccacaag aacgccatc cgagcgata 
cgaaaaatc aaatatttc gtcgccaga cgcaagttc 
cagcaggcg cgcaagccc tacgacgtg cgggacgtc 
atcgagcag tacagccaa ggccacctc aacatgatg 
gtccgcatc aaggagtta cagcgacgg ctggaccag 
acgctgggc aaacctggg agttaccta gcggggatc 
gatcgcgtt ggaaacgtc aaacccatg acggtgggt 
gcgagactg taccgagtg gaacagcag ctggggacc 
atggacaag aagctggac gccttaact cacattctg 
aactcgttg gcacaaaaa aatcaagct gcgccactt 
aggtcgatc gaagatgat gtctga
    mRNA sequence
    • >TC007822 RA
      gtcgttgaa taatccagt gcacattgt tgtgccaag 
gatttttat ccggcaatt agcgtctaa agttgtcac 
cccctggct tcctggcgc ttaaagtcg acctacctg 
atttgattc cgccgtggc ttagatcga tgtgagcgt 
cgtcggcgt cgtcatcgt gtgagcgtc cctgcgcga 
gaccaacag ttggttttc ggacgcgtt ggaagccgc 
caccgccac cgccgccgc cgccgccgc caccgttgc 
attattcag cgcgaccat tacgagttg tttcgtcga 
taaatcggg ggctctgcc ccccgcgcc gccctaatt 
tggcggagg cgccgggat aagggggcc cgactcgcg 
gtatgacac gggttacgg atgtggggc ttttgccgc 
ccaatttcg aactgtggg cttttggtt gaggtgatc 
acgcacccc ctccgacga tgccgtcgt gatgcctcc 
gttcgtgtc ggcccggcc cccgttcgc aaatgctgt 
cattagtga tgttaagcg agatgaatc ggtctgaac 
aggaaagct tgtaccgca cgtcccgca aactgagct 
tggtgccga tcggcagct cgccgcacg cgggcgccc 
cctccgacg acctggtgc tgagcgtgc tgaagagtc 
ccgtgaacc acgtggcgc gcgatgagg aggcgcccc 
aggtgccca gcgaggagg acgagtgct ccgagcgcc 
tgcccttcc tgcagcgcc tgcggggcc accggccgc 
gcttcggcg aagcgcacc acaagcgcg tcccgacgc 
cgctcaagc gcaagcagc gcttccgca gccacaaga 
acgacgtgg tgcagcccg aggacgtcg tcgacgagg 
aggaggacg tggcggcgc cgacggacg agcccaggg 
gcgtccccg acccctact atcccattt atttgccca 
tcgatcagg cgttcaagg ccaagtatg ttttccacc 
acaagaagg ggaagacgt gccaggaga ggacgtacg 
tttttctgg agcatcccg gaggatggc tgtgcttta 
tctatcact ttactgtgt ttatgttag tcctcgttt 
gcctaatat tcagcgtgt tgtcaacga tcgacacgt 
accaaagct ttgccaacg agacccttt tctggatgg 
agatttgcc tggtggttt tcttcggcg ttgagtatt 
tggtgcggc tgtggtcgg caggctgca ggtcaaagt 
acatgggct tttgggggc gccttcgat ttatacgca 
aacccatct gcattatcg atttaattg tagtggtag 
cttcgattg tagttctaa cgttaggtt caaacgggc 
aagtgtttg ccacgtcgg ctatccgcg gaattcgct 
tcctgcaga ttttgcgga tgctccacg tggacaggc 
aagggggca cgtggaggc tgctgggtt cagttgtgt 
tcatacatc gacaagaat taataacga ctctttaca 
tcggatttc tgggcctaa ttttcagca gttatttcg 
tttatttgg cggagaagg acgctgtgg gtgctgatg 
gaaaaaata cgggggatt tttcgagtt atgcggatg 
cgctctggt ggggggtta tcactgtta cgaccattg 
gatatgggg acactgtgc ccaggactt ggatgggca 
agattgtag cgtcgtgtt ttagcgtct ttgccatat 
ctttcttcg ctcttccag ccggcatcc tcggctcgg 
gcttcgccc tcaaggtgc agcagaagc aacgccaga 
agcatttca atcggcaga ttcctgccg ccgccatgc 
tcatccagt gtttgtggc ggtgttacg ccgccgaca 
aaagtttca attcgaggg caacgtggc agatttatc 
tcaaggata cgaacggct tcaccacca acaactctt 
ccagtggga gcggcacca attgtaaca tacctcttt 
ccaaggtgg ctaagaaag cgtccgttt tgaaacgtc 
ggaaatcgc ggaatcgca tggaggcgc ctggaacgc 
cacagggcg acacccccg tccaagcag tcgccccca 
atgcaccct ccaggtgcg agtccgatt ccgacgttg 
ttttctaca tggaggagc ctaaagccg gcaccccaa 
accgcgtcc gccgaggcg aacgtgaaa gctcccgtg 
gagccgtct ttaccagcc agaccagca ccgtcacgg 
aggccgcca gcgacgaca tcgacgacc tcgacggcg 
agctccccc gggtgacac aactcacag aagcccaca 
agaacgcca tccgagcga tacgaaaaa tcaaatatt 
tcgtcgcca gacgcaagt tccagcagg cgcgcaagc 
cctacgacg tgcgggacg tcatcgagc agtacagcc 
aaggccacc tcaacatga tggtccgca tcaaggagt 
tacagcgac ggctggacc agacgctgg gcaaacctg 
ggagttacc tagcgggga tcgatcgcg ttggaaacg 
tcaaaccca tgacggtgg gtgcgagac tgtaccgag 
tggaacagc agctgggga ccatggaca agaagctgg 
acgccttaa ctcacattc tgaactcgt tggcacaaa 
aaaatcaag ctgcgccac ttaggtcga tcgaagatg 
atgtctgag caataatct tgcaactac atatccagt 
gggattggc taacttact gtacatatg agtagttta 
agggaattt agtcaatat tcaaaacta agcatggta 
ttatggggc acacgtgta aattgatca aggatcaat 
tgtaatata attatattt ttagccact tttagatta 
tttccatat ctatatcta gaccgtgcc tatttacta 
gatttatat gagaattaa aatagggag cgtacgagc 
ggtctcgaa cacatgcag gcgtttttc ataacggaa 
tcaaaattt catgttgca gacaatcgt tacgaggta 
agttaggga taagatttg atgcaagac aaggtaggt 
ttggtttca catgaaatt attataaaa caacctctc 
tgatagagc tcttttctc aaaaaaaat aaataaaat 
aaacgaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY