Status
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Gene information
General information
TC032726 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 580869..609761
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032726
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02749

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032726 RA
      MLPLATFFC VLFLSNGLF SDVSGLQDS SLNSGAVSH 
FEPQVAMLC DAGPHGQEA YHPKYMTEQ GEWLSDLYS 
KATCFKDKM DILNYCKKV YPKRDITNI VESSHYLKI 
ASWCKAGST PGAQARGKC KTARWVKPF RCLEGPFQS 
DALLVPENC LFDHIHNQS NCWTFGRWN ATAGNACQE 
RGLQLRSFA MLLPCGISL FSGVEFVCC PKHFKDNMK 
PKKPLDLTI LKKDQDNIF DTIDDDDST SDEDDDKDD 
LDDLADDTE LADDPNIDD EDDDDDEYD DSDWDTTPS 
STTSKQSTT TTTTTTTAK TTSVPTPDP YFTHFDPRN 
EHQSYKEAQ ERLEETHRE KVTRVMKDW SDLEERYQD 
MRQTDPRGA DEFKQRMTQ RFQQTVQAL EEEGDAEKH 
QLAAMHQQR VLAHINQRK KEAMTCYTQ ALNDAPLNT 
HRVQKCLEK LLRSLHKDR AHAINHYRH LLASSLEAA 
AREQPRILE RLTDIDRTV NQSMQMLKR YPELATKIG 
QLMDDYIQA LRSKDETPG SLLAMTTEA EAAILDKYK 
SEVTNKQAE RERQRLQEK ERKEQRKKE RSELREEKK 
RVEAVLGKK ISNFDDEDM EDESLPEER TTIISTTGS 
STFDLVSNS IPTTSHRDA VDDAAVQQA VEDVAAVVH 
KQDVIRVAH AMAHDISHG EPTYSVRRE VYGSKDGKS 
VYFTLAFAG MALMAAVVV GVGVARRRS ARSPQSQGF 
VEVDQAATP EERHVANMQ INGYENPTY KYFEVKE
    CDS sequence
    • >TC032726 RA
      atgttgcca ttagcgact tttttttgt gtgttattt 
ctatcaaat ggacttttt agcgatgtc tcgggcctt 
caagacagt agcttaaac agcggggct gtgtcccac 
ttcgaacct caggtagca atgctttgt gatgcgggc 
ccccatggc caagaggca tatcatcca aagtatatg 
acagagcaa ggggaatgg ctctcagat ctctactcc 
aaagccacg tgttttaag gacaaaatg gatattttg 
aactattgc aaaaaggtg taccccaag cgagatatc 
acaaacatc gtcgaaagc agtcattat ttgaagatt 
gcctcctgg tgcaaagcc ggctccaca cctggggca 
caggctagg ggcaagtgc aagactgcc agatgggtc 
aaaccgttc cggtgtcta gagggtccg ttccagtcg 
gacgctctc ctagtgccc gaaaactgc ttattcgac 
cacatccac aaccagagc aactgctgg acattcggt 
cgctggaat gccactgca ggcaacgct tgccaagaa 
cgaggactc caacttcga tcgtttgct atgcttctt 
ccctgtggc atttctttg tttagtggt gtggaattt 
gtatgctgt cccaaacac ttcaaagac aacatgaag 
ccgaaaaag ccactggac ttaacaatc ctgaaaaag 
gaccaggac aacatcttc gacaccatc gacgacgat 
gactccact tcggacgaa gacgacgac aaggacgac 
ctggacgac ctcgccgac gacaccgag ctcgccgac 
gaccccaac atcgacgac gaggacgac gatgacgac 
gaatacgac gactcggat tgggacacc accccgtcg 
agcacgacg tccaaacaa tccaccacc acgaccacc 
accaccacc acggcgaag acgacgtca gtgccgacc 
ccagacccc tatttcacg catttcgat ccgagaaac 
gagcaccag agttacaag gaggcgcag gaacgtttg 
gaggagaca cacagggag aaagttaca agggttatg 
aaggattgg tcggacctg gaggagagg tatcaggac 
atgagacag acggatcca cgtggagct gatgagttc 
aagcagagg atgacgcag aggtttcaa cagacggtg 
caagcgctt gaagaagag ggcgatgct gagaaacat 
cagttggca gcaatgcat cagcaaaga gtcctagca 
catattaat caaaggaag aaggaggcc atgacttgt 
tatacacaa gcgttgaat gatgcgcct ctcaatacc 
catcgtgtg cagaaatgc ctggaaaaa ctgctccgt 
tctcttcac aaagaccga gctcatgct attaaccac 
taccgccat ctgttggca agtagtttg gaggcggca 
gctcgtgaa caaccgcgc attctcgaa cgtttaaca 
gacattgat cgtacggtt aatcaaagc atgcaaatg 
ttaaaaaga tatccagaa ttggcgacg aaaatcggt 
caattaatg gatgattac atccaagct ttgagatca 
aaagatgaa acacctgga tcacttcta gcgatgaca 
actgaagct gaagctgcg attttggat aaatataaa 
tcagaagtt acaaataaa caagcagag agagaacga 
caaaggttg caagagaag gagaggaag gaacagagg 
aagaaagaa cggtcggaa ttaagggag gagaagaag 
agagttgag gctgtttta gggaagaag attagcaat 
tttgatgat gaagacatg gaagatgag tcgttgcca 
gaagaaagg acaacaata atttcgacc accgggagc 
tcaactttc gaccttgtg agcaactcc atccctacc 
acaagccac cgcgacgcc gtggacgac gccgccgtc 
cagcaagcc gtcgaagac gtcgccgcc gtggtccac 
aaacaagac gtgatccga gtcgcccat gcaatggcg 
catgacatc agccacggc gaacctact tattcagtc 
cgacgtgag gtgtacggc agcaaagac ggcaaaagt 
gtttacttc actctcgcc ttcgccggc atggcgctg 
atggccgcc gtggtggtg ggggtgggg gtggcacgg 
cgcaggtcc gcacgctcc ccccaaagc caaggcttc 
gtggaggtg gaccaggcc gccaccccc gaggagcgg 
cacgtggcc aatatgcaa atcaatggc tacgaaaat 
cccacctac aaatatttc gaagtcaag gagtaa
    mRNA sequence
    • >TC032726 RA
      gtggagctg tcgtcagtc gtgaaacca cccatcgga 
gttgcgttt cagtgttgt gtgctttct gtgagaaat 
ccaccaaaa atgttgcca ttagcgact tttttttgt 
gtgttattt ctatcaaat ggacttttt agcgatgtc 
tcgggcctt caagacagt agcttaaac agcggggct 
gtgtcccac ttcgaacct caggtagca atgctttgt 
gatgcgggc ccccatggc caagaggca tatcatcca 
aagtatatg acagagcaa ggggaatgg ctctcagat 
ctctactcc aaagccacg tgttttaag gacaaaatg 
gatattttg aactattgc aaaaaggtg taccccaag 
cgagatatc acaaacatc gtcgaaagc agtcattat 
ttgaagatt gcctcctgg tgcaaagcc ggctccaca 
cctggggca caggctagg ggcaagtgc aagactgcc 
agatgggtc aaaccgttc cggtgtcta gagggtccg 
ttccagtcg gacgctctc ctagtgccc gaaaactgc 
ttattcgac cacatccac aaccagagc aactgctgg 
acattcggt cgctggaat gccactgca ggcaacgct 
tgccaagaa cgaggactc caacttcga tcgtttgct 
atgcttctt ccctgtggc atttctttg tttagtggt 
gtggaattt gtatgctgt cccaaacac ttcaaagac 
aacatgaag ccgaaaaag ccactggac ttaacaatc 
ctgaaaaag gaccaggac aacatcttc gacaccatc 
gacgacgat gactccact tcggacgaa gacgacgac 
aaggacgac ctggacgac ctcgccgac gacaccgag 
ctcgccgac gaccccaac atcgacgac gaggacgac 
gatgacgac gaatacgac gactcggat tgggacacc 
accccgtcg agcacgacg tccaaacaa tccaccacc 
acgaccacc accaccacc acggcgaag acgacgtca 
gtgccgacc ccagacccc tatttcacg catttcgat 
ccgagaaac gagcaccag agttacaag gaggcgcag 
gaacgtttg gaggagaca cacagggag aaagttaca 
agggttatg aaggattgg tcggacctg gaggagagg 
tatcaggac atgagacag acggatcca cgtggagct 
gatgagttc aagcagagg atgacgcag aggtttcaa 
cagacggtg caagcgctt gaagaagag ggcgatgct 
gagaaacat cagttggca gcaatgcat cagcaaaga 
gtcctagca catattaat caaaggaag aaggaggcc 
atgacttgt tatacacaa gcgttgaat gatgcgcct 
ctcaatacc catcgtgtg cagaaatgc ctggaaaaa 
ctgctccgt tctcttcac aaagaccga gctcatgct 
attaaccac taccgccat ctgttggca agtagtttg 
gaggcggca gctcgtgaa caaccgcgc attctcgaa 
cgtttaaca gacattgat cgtacggtt aatcaaagc 
atgcaaatg ttaaaaaga tatccagaa ttggcgacg 
aaaatcggt caattaatg gatgattac atccaagct 
ttgagatca aaagatgaa acacctgga tcacttcta 
gcgatgaca actgaagct gaagctgcg attttggat 
aaatataaa tcagaagtt acaaataaa caagcagag 
agagaacga caaaggttg caagagaag gagaggaag 
gaacagagg aagaaagaa cggtcggaa ttaagggag 
gagaagaag agagttgag gctgtttta gggaagaag 
attagcaat tttgatgat gaagacatg gaagatgag 
tcgttgcca gaagaaagg acaacaata atttcgacc 
accgggagc tcaactttc gaccttgtg agcaactcc 
atccctacc acaagccac cgcgacgcc gtggacgac 
gccgccgtc cagcaagcc gtcgaagac gtcgccgcc 
gtggtccac aaacaagac gtgatccga gtcgcccat 
gcaatggcg catgacatc agccacggc gaacctact 
tattcagtc cgacgtgag gtgtacggc agcaaagac 
ggcaaaagt gtttacttc actctcgcc ttcgccggc 
atggcgctg atggccgcc gtggtggtg ggggtgggg 
gtggcacgg cgcaggtcc gcacgctcc ccccaaagc 
caaggcttc gtggaggtg gaccaggcc gccaccccc 
gaggagcgg cacgtggcc aatatgcaa atcaatggc 
tacgaaaat cccacctac aaatatttc gaagtcaag 
gagtaataa acgatttgg ttgcgtttg gttggttag 
gttggtatt ttttacgtc attccattc gttttatta 
cagtgtact taactaagc aactgataa taataattc 
ggacatatg caaaaacgt attttccaa gtctaaaaa 
ttatgaaat aagtaataa tttttgcat atgacagcc 
agtccatgt tttacactt attgtataa tcttaaggc 
gagctgtgg agttacatt gccttggtg taattttac 
agtggaaga gtgtagcta gatgtatgg taccctcca 
taatatgtg acagcgttt ctctcttaa ggcctacgt 
ttcaaactt tcaataatg cagctagag attgaactc 
aaaatgact gagctacta gaaattggc gccaatttt 
aaattattt atgacgtat ggcaatttt ctggcaaaa 
ttttcgttt tcctaactt gaaaaattg tcaaaaagc 
cgtcaggtt ggcattacc ataagtaat tttttgttg 
ttaaagttg gtacttttt tttactgta ctacagtga 
cctgatttt tttgtacaa aatacaaaa tacgaaaat 
aactttaaa aattacaga catgttttt cgtatagtc 
tgtcatact tttgacaat tgtcaaaac atgactgat 
cagtttctt tttgataat taaatgttt gaagagcct 
tctagaatt tacaaaact gtcaactgt caaaattca 
aggctagta tgttttttt aaaatggga cggccatat 
tttttaacg gcaatagaa ctaaagttg acatttatg 
gtaattttt atcatcacg ttctatacc tatctcagt 
ttttgaaaa aaatcacga gaaacggat tttttatat 
cattactat aatcatttt ttgattatc tttttaaaa 
taataaaaa aaatcatag tagctttgc aatttgaca 
gttgacaag tttaaaaat tttggaaga ttgtttaat 
cgtttggta acaaatcca agaagaatt gtgatgttc 
tgaatgaga aaaaaattg atatatgtc aaaactctg 
acagatatg tgcaaagtt ggcgccatt ttggatcaa 
atttattgg ttggatcag tgtgtgtgc aaaataata 
agtttaaag tttgtgatg taggcccta gaagagtcg 
ccctgtact taattagaa tataacaag tgcatgttc 
aaccaacaa gctttttgt acaaagtct ttttacttt 
ttaccagct tgcaatcac ccgaatttg gcacaatcc 
gaacacttg tatgttttt tgtttgaga caaaacgaa 
agaaatgca agtgttcat cacaacgag acgtatttt 
cacgttgtc gaaagcttg atgaacaat tgtagataa 
cacaaatca tagaatttg tacaaaagt tgttgcgta 
cattctttt ttttaaaca ttgcgcgac aatgaggct 
tctgacagc ttaatgtgt acagtctag aatacaaaa 
aaaaaaaca accaacaac tttgtctta ctttctccc 
gtcttgtag gttttcaaa aactagata tgcgaatgt 
aaaattgat aaaagcgtg tttgatgtg catatagtt 
taatactcg attagaatt atttttcaa aacctgttt 
catataaga attattgta ttatatttc ttttctacg 
cgttttatt agtacgttt ctcctcatc ctgaagtaa 
ctagactag tttagcata cttgatagt taattatat 
gttttgtgg ccatgtaat tttaaattt aatgtaata 
aagaactaa attatta

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY