Status
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Gene information
General information
TC032726 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 580869..609761
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032726
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02749

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032726 RA
      MLPLATFFC VLFLSNGLF SDVSGLQDS SLNSGAVSH 
      FEPQVAMLC DAGPHGQEA YHPKYMTEQ GEWLSDLYS 
      KATCFKDKM DILNYCKKV YPKRDITNI VESSHYLKI 
      ASWCKAGST PGAQARGKC KTARWVKPF RCLEGPFQS 
      DALLVPENC LFDHIHNQS NCWTFGRWN ATAGNACQE 
      RGLQLRSFA MLLPCGISL FSGVEFVCC PKHFKDNMK 
      PKKPLDLTI LKKDQDNIF DTIDDDDST SDEDDDKDD 
      LDDLADDTE LADDPNIDD EDDDDDEYD DSDWDTTPS 
      STTSKQSTT TTTTTTTAK TTSVPTPDP YFTHFDPRN 
      EHQSYKEAQ ERLEETHRE KVTRVMKDW SDLEERYQD 
      MRQTDPRGA DEFKQRMTQ RFQQTVQAL EEEGDAEKH 
      QLAAMHQQR VLAHINQRK KEAMTCYTQ ALNDAPLNT 
      HRVQKCLEK LLRSLHKDR AHAINHYRH LLASSLEAA 
      AREQPRILE RLTDIDRTV NQSMQMLKR YPELATKIG 
      QLMDDYIQA LRSKDETPG SLLAMTTEA EAAILDKYK 
      SEVTNKQAE RERQRLQEK ERKEQRKKE RSELREEKK 
      RVEAVLGKK ISNFDDEDM EDESLPEER TTIISTTGS 
      STFDLVSNS IPTTSHRDA VDDAAVQQA VEDVAAVVH 
      KQDVIRVAH AMAHDISHG EPTYSVRRE VYGSKDGKS 
      VYFTLAFAG MALMAAVVV GVGVARRRS ARSPQSQGF 
      VEVDQAATP EERHVANMQ INGYENPTY KYFEVKE
    CDS sequence
    • >TC032726 RA
      atgttgcca ttagcgact tttttttgt gtgttattt 
      ctatcaaat ggacttttt agcgatgtc tcgggcctt 
      caagacagt agcttaaac agcggggct gtgtcccac 
      ttcgaacct caggtagca atgctttgt gatgcgggc 
      ccccatggc caagaggca tatcatcca aagtatatg 
      acagagcaa ggggaatgg ctctcagat ctctactcc 
      aaagccacg tgttttaag gacaaaatg gatattttg 
      aactattgc aaaaaggtg taccccaag cgagatatc 
      acaaacatc gtcgaaagc agtcattat ttgaagatt 
      gcctcctgg tgcaaagcc ggctccaca cctggggca 
      caggctagg ggcaagtgc aagactgcc agatgggtc 
      aaaccgttc cggtgtcta gagggtccg ttccagtcg 
      gacgctctc ctagtgccc gaaaactgc ttattcgac 
      cacatccac aaccagagc aactgctgg acattcggt 
      cgctggaat gccactgca ggcaacgct tgccaagaa 
      cgaggactc caacttcga tcgtttgct atgcttctt 
      ccctgtggc atttctttg tttagtggt gtggaattt 
      gtatgctgt cccaaacac ttcaaagac aacatgaag 
      ccgaaaaag ccactggac ttaacaatc ctgaaaaag 
      gaccaggac aacatcttc gacaccatc gacgacgat 
      gactccact tcggacgaa gacgacgac aaggacgac 
      ctggacgac ctcgccgac gacaccgag ctcgccgac 
      gaccccaac atcgacgac gaggacgac gatgacgac 
      gaatacgac gactcggat tgggacacc accccgtcg 
      agcacgacg tccaaacaa tccaccacc acgaccacc 
      accaccacc acggcgaag acgacgtca gtgccgacc 
      ccagacccc tatttcacg catttcgat ccgagaaac 
      gagcaccag agttacaag gaggcgcag gaacgtttg 
      gaggagaca cacagggag aaagttaca agggttatg 
      aaggattgg tcggacctg gaggagagg tatcaggac 
      atgagacag acggatcca cgtggagct gatgagttc 
      aagcagagg atgacgcag aggtttcaa cagacggtg 
      caagcgctt gaagaagag ggcgatgct gagaaacat 
      cagttggca gcaatgcat cagcaaaga gtcctagca 
      catattaat caaaggaag aaggaggcc atgacttgt 
      tatacacaa gcgttgaat gatgcgcct ctcaatacc 
      catcgtgtg cagaaatgc ctggaaaaa ctgctccgt 
      tctcttcac aaagaccga gctcatgct attaaccac 
      taccgccat ctgttggca agtagtttg gaggcggca 
      gctcgtgaa caaccgcgc attctcgaa cgtttaaca 
      gacattgat cgtacggtt aatcaaagc atgcaaatg 
      ttaaaaaga tatccagaa ttggcgacg aaaatcggt 
      caattaatg gatgattac atccaagct ttgagatca 
      aaagatgaa acacctgga tcacttcta gcgatgaca 
      actgaagct gaagctgcg attttggat aaatataaa 
      tcagaagtt acaaataaa caagcagag agagaacga 
      caaaggttg caagagaag gagaggaag gaacagagg 
      aagaaagaa cggtcggaa ttaagggag gagaagaag 
      agagttgag gctgtttta gggaagaag attagcaat 
      tttgatgat gaagacatg gaagatgag tcgttgcca 
      gaagaaagg acaacaata atttcgacc accgggagc 
      tcaactttc gaccttgtg agcaactcc atccctacc 
      acaagccac cgcgacgcc gtggacgac gccgccgtc 
      cagcaagcc gtcgaagac gtcgccgcc gtggtccac 
      aaacaagac gtgatccga gtcgcccat gcaatggcg 
      catgacatc agccacggc gaacctact tattcagtc 
      cgacgtgag gtgtacggc agcaaagac ggcaaaagt 
      gtttacttc actctcgcc ttcgccggc atggcgctg 
      atggccgcc gtggtggtg ggggtgggg gtggcacgg 
      cgcaggtcc gcacgctcc ccccaaagc caaggcttc 
      gtggaggtg gaccaggcc gccaccccc gaggagcgg 
      cacgtggcc aatatgcaa atcaatggc tacgaaaat 
      cccacctac aaatatttc gaagtcaag gagtaa
    mRNA sequence
    • >TC032726 RA
      gtggagctg tcgtcagtc gtgaaacca cccatcgga 
      gttgcgttt cagtgttgt gtgctttct gtgagaaat 
      ccaccaaaa atgttgcca ttagcgact tttttttgt 
      gtgttattt ctatcaaat ggacttttt agcgatgtc 
      tcgggcctt caagacagt agcttaaac agcggggct 
      gtgtcccac ttcgaacct caggtagca atgctttgt 
      gatgcgggc ccccatggc caagaggca tatcatcca 
      aagtatatg acagagcaa ggggaatgg ctctcagat 
      ctctactcc aaagccacg tgttttaag gacaaaatg 
      gatattttg aactattgc aaaaaggtg taccccaag 
      cgagatatc acaaacatc gtcgaaagc agtcattat 
      ttgaagatt gcctcctgg tgcaaagcc ggctccaca 
      cctggggca caggctagg ggcaagtgc aagactgcc 
      agatgggtc aaaccgttc cggtgtcta gagggtccg 
      ttccagtcg gacgctctc ctagtgccc gaaaactgc 
      ttattcgac cacatccac aaccagagc aactgctgg 
      acattcggt cgctggaat gccactgca ggcaacgct 
      tgccaagaa cgaggactc caacttcga tcgtttgct 
      atgcttctt ccctgtggc atttctttg tttagtggt 
      gtggaattt gtatgctgt cccaaacac ttcaaagac 
      aacatgaag ccgaaaaag ccactggac ttaacaatc 
      ctgaaaaag gaccaggac aacatcttc gacaccatc 
      gacgacgat gactccact tcggacgaa gacgacgac 
      aaggacgac ctggacgac ctcgccgac gacaccgag 
      ctcgccgac gaccccaac atcgacgac gaggacgac 
      gatgacgac gaatacgac gactcggat tgggacacc 
      accccgtcg agcacgacg tccaaacaa tccaccacc 
      acgaccacc accaccacc acggcgaag acgacgtca 
      gtgccgacc ccagacccc tatttcacg catttcgat 
      ccgagaaac gagcaccag agttacaag gaggcgcag 
      gaacgtttg gaggagaca cacagggag aaagttaca 
      agggttatg aaggattgg tcggacctg gaggagagg 
      tatcaggac atgagacag acggatcca cgtggagct 
      gatgagttc aagcagagg atgacgcag aggtttcaa 
      cagacggtg caagcgctt gaagaagag ggcgatgct 
      gagaaacat cagttggca gcaatgcat cagcaaaga 
      gtcctagca catattaat caaaggaag aaggaggcc 
      atgacttgt tatacacaa gcgttgaat gatgcgcct 
      ctcaatacc catcgtgtg cagaaatgc ctggaaaaa 
      ctgctccgt tctcttcac aaagaccga gctcatgct 
      attaaccac taccgccat ctgttggca agtagtttg 
      gaggcggca gctcgtgaa caaccgcgc attctcgaa 
      cgtttaaca gacattgat cgtacggtt aatcaaagc 
      atgcaaatg ttaaaaaga tatccagaa ttggcgacg 
      aaaatcggt caattaatg gatgattac atccaagct 
      ttgagatca aaagatgaa acacctgga tcacttcta 
      gcgatgaca actgaagct gaagctgcg attttggat 
      aaatataaa tcagaagtt acaaataaa caagcagag 
      agagaacga caaaggttg caagagaag gagaggaag 
      gaacagagg aagaaagaa cggtcggaa ttaagggag 
      gagaagaag agagttgag gctgtttta gggaagaag 
      attagcaat tttgatgat gaagacatg gaagatgag 
      tcgttgcca gaagaaagg acaacaata atttcgacc 
      accgggagc tcaactttc gaccttgtg agcaactcc 
      atccctacc acaagccac cgcgacgcc gtggacgac 
      gccgccgtc cagcaagcc gtcgaagac gtcgccgcc 
      gtggtccac aaacaagac gtgatccga gtcgcccat 
      gcaatggcg catgacatc agccacggc gaacctact 
      tattcagtc cgacgtgag gtgtacggc agcaaagac 
      ggcaaaagt gtttacttc actctcgcc ttcgccggc 
      atggcgctg atggccgcc gtggtggtg ggggtgggg 
      gtggcacgg cgcaggtcc gcacgctcc ccccaaagc 
      caaggcttc gtggaggtg gaccaggcc gccaccccc 
      gaggagcgg cacgtggcc aatatgcaa atcaatggc 
      tacgaaaat cccacctac aaatatttc gaagtcaag 
      gagtaataa acgatttgg ttgcgtttg gttggttag 
      gttggtatt ttttacgtc attccattc gttttatta 
      cagtgtact taactaagc aactgataa taataattc 
      ggacatatg caaaaacgt attttccaa gtctaaaaa 
      ttatgaaat aagtaataa tttttgcat atgacagcc 
      agtccatgt tttacactt attgtataa tcttaaggc 
      gagctgtgg agttacatt gccttggtg taattttac 
      agtggaaga gtgtagcta gatgtatgg taccctcca 
      taatatgtg acagcgttt ctctcttaa ggcctacgt 
      ttcaaactt tcaataatg cagctagag attgaactc 
      aaaatgact gagctacta gaaattggc gccaatttt 
      aaattattt atgacgtat ggcaatttt ctggcaaaa 
      ttttcgttt tcctaactt gaaaaattg tcaaaaagc 
      cgtcaggtt ggcattacc ataagtaat tttttgttg 
      ttaaagttg gtacttttt tttactgta ctacagtga 
      cctgatttt tttgtacaa aatacaaaa tacgaaaat 
      aactttaaa aattacaga catgttttt cgtatagtc 
      tgtcatact tttgacaat tgtcaaaac atgactgat 
      cagtttctt tttgataat taaatgttt gaagagcct 
      tctagaatt tacaaaact gtcaactgt caaaattca 
      aggctagta tgttttttt aaaatggga cggccatat 
      tttttaacg gcaatagaa ctaaagttg acatttatg 
      gtaattttt atcatcacg ttctatacc tatctcagt 
      ttttgaaaa aaatcacga gaaacggat tttttatat 
      cattactat aatcatttt ttgattatc tttttaaaa 
      taataaaaa aaatcatag tagctttgc aatttgaca 
      gttgacaag tttaaaaat tttggaaga ttgtttaat 
      cgtttggta acaaatcca agaagaatt gtgatgttc 
      tgaatgaga aaaaaattg atatatgtc aaaactctg 
      acagatatg tgcaaagtt ggcgccatt ttggatcaa 
      atttattgg ttggatcag tgtgtgtgc aaaataata 
      agtttaaag tttgtgatg taggcccta gaagagtcg 
      ccctgtact taattagaa tataacaag tgcatgttc 
      aaccaacaa gctttttgt acaaagtct ttttacttt 
      ttaccagct tgcaatcac ccgaatttg gcacaatcc 
      gaacacttg tatgttttt tgtttgaga caaaacgaa 
      agaaatgca agtgttcat cacaacgag acgtatttt 
      cacgttgtc gaaagcttg atgaacaat tgtagataa 
      cacaaatca tagaatttg tacaaaagt tgttgcgta 
      cattctttt ttttaaaca ttgcgcgac aatgaggct 
      tctgacagc ttaatgtgt acagtctag aatacaaaa 
      aaaaaaaca accaacaac tttgtctta ctttctccc 
      gtcttgtag gttttcaaa aactagata tgcgaatgt 
      aaaattgat aaaagcgtg tttgatgtg catatagtt 
      taatactcg attagaatt atttttcaa aacctgttt 
      catataaga attattgta ttatatttc ttttctacg 
      cgttttatt agtacgttt ctcctcatc ctgaagtaa 
      ctagactag tttagcata cttgatagt taattatat 
      gttttgtgg ccatgtaat tttaaattt aatgtaata 
      aagaactaa attatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY