| General information | |||
| TC032726 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 580869..609761 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC032726 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02749 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
- >TC032726 RA
MLPLATFFC VLFLSNGLF SDVSGLQDS SLNSGAVSH FEPQVAMLC DAGPHGQEA YHPKYMTEQ GEWLSDLYS KATCFKDKM DILNYCKKV YPKRDITNI VESSHYLKI ASWCKAGST PGAQARGKC KTARWVKPF RCLEGPFQS DALLVPENC LFDHIHNQS NCWTFGRWN ATAGNACQE RGLQLRSFA MLLPCGISL FSGVEFVCC PKHFKDNMK PKKPLDLTI LKKDQDNIF DTIDDDDST SDEDDDKDD LDDLADDTE LADDPNIDD EDDDDDEYD DSDWDTTPS STTSKQSTT TTTTTTTAK TTSVPTPDP YFTHFDPRN EHQSYKEAQ ERLEETHRE KVTRVMKDW SDLEERYQD MRQTDPRGA DEFKQRMTQ RFQQTVQAL EEEGDAEKH QLAAMHQQR VLAHINQRK KEAMTCYTQ ALNDAPLNT HRVQKCLEK LLRSLHKDR AHAINHYRH LLASSLEAA AREQPRILE RLTDIDRTV NQSMQMLKR YPELATKIG QLMDDYIQA LRSKDETPG SLLAMTTEA EAAILDKYK SEVTNKQAE RERQRLQEK ERKEQRKKE RSELREEKK RVEAVLGKK ISNFDDEDM EDESLPEER TTIISTTGS STFDLVSNS IPTTSHRDA VDDAAVQQA VEDVAAVVH KQDVIRVAH AMAHDISHG EPTYSVRRE VYGSKDGKS VYFTLAFAG MALMAAVVV GVGVARRRS ARSPQSQGF VEVDQAATP EERHVANMQ INGYENPTY KYFEVKE
- >TC032726 RA
atgttgcca ttagcgact tttttttgt gtgttattt ctatcaaat ggacttttt agcgatgtc tcgggcctt caagacagt agcttaaac agcggggct gtgtcccac ttcgaacct caggtagca atgctttgt gatgcgggc ccccatggc caagaggca tatcatcca aagtatatg acagagcaa ggggaatgg ctctcagat ctctactcc aaagccacg tgttttaag gacaaaatg gatattttg aactattgc aaaaaggtg taccccaag cgagatatc acaaacatc gtcgaaagc agtcattat ttgaagatt gcctcctgg tgcaaagcc ggctccaca cctggggca caggctagg ggcaagtgc aagactgcc agatgggtc aaaccgttc cggtgtcta gagggtccg ttccagtcg gacgctctc ctagtgccc gaaaactgc ttattcgac cacatccac aaccagagc aactgctgg acattcggt cgctggaat gccactgca ggcaacgct tgccaagaa cgaggactc caacttcga tcgtttgct atgcttctt ccctgtggc atttctttg tttagtggt gtggaattt gtatgctgt cccaaacac ttcaaagac aacatgaag ccgaaaaag ccactggac ttaacaatc ctgaaaaag gaccaggac aacatcttc gacaccatc gacgacgat gactccact tcggacgaa gacgacgac aaggacgac ctggacgac ctcgccgac gacaccgag ctcgccgac gaccccaac atcgacgac gaggacgac gatgacgac gaatacgac gactcggat tgggacacc accccgtcg agcacgacg tccaaacaa tccaccacc acgaccacc accaccacc acggcgaag acgacgtca gtgccgacc ccagacccc tatttcacg catttcgat ccgagaaac gagcaccag agttacaag gaggcgcag gaacgtttg gaggagaca cacagggag aaagttaca agggttatg aaggattgg tcggacctg gaggagagg tatcaggac atgagacag acggatcca cgtggagct gatgagttc aagcagagg atgacgcag aggtttcaa cagacggtg caagcgctt gaagaagag ggcgatgct gagaaacat cagttggca gcaatgcat cagcaaaga gtcctagca catattaat caaaggaag aaggaggcc atgacttgt tatacacaa gcgttgaat gatgcgcct ctcaatacc catcgtgtg cagaaatgc ctggaaaaa ctgctccgt tctcttcac aaagaccga gctcatgct attaaccac taccgccat ctgttggca agtagtttg gaggcggca gctcgtgaa caaccgcgc attctcgaa cgtttaaca gacattgat cgtacggtt aatcaaagc atgcaaatg ttaaaaaga tatccagaa ttggcgacg aaaatcggt caattaatg gatgattac atccaagct ttgagatca aaagatgaa acacctgga tcacttcta gcgatgaca actgaagct gaagctgcg attttggat aaatataaa tcagaagtt acaaataaa caagcagag agagaacga caaaggttg caagagaag gagaggaag gaacagagg aagaaagaa cggtcggaa ttaagggag gagaagaag agagttgag gctgtttta gggaagaag attagcaat tttgatgat gaagacatg gaagatgag tcgttgcca gaagaaagg acaacaata atttcgacc accgggagc tcaactttc gaccttgtg agcaactcc atccctacc acaagccac cgcgacgcc gtggacgac gccgccgtc cagcaagcc gtcgaagac gtcgccgcc gtggtccac aaacaagac gtgatccga gtcgcccat gcaatggcg catgacatc agccacggc gaacctact tattcagtc cgacgtgag gtgtacggc agcaaagac ggcaaaagt gtttacttc actctcgcc ttcgccggc atggcgctg atggccgcc gtggtggtg ggggtgggg gtggcacgg cgcaggtcc gcacgctcc ccccaaagc caaggcttc gtggaggtg gaccaggcc gccaccccc gaggagcgg cacgtggcc aatatgcaa atcaatggc tacgaaaat cccacctac aaatatttc gaagtcaag gagtaa
- >TC032726 RA
gtggagctg tcgtcagtc gtgaaacca cccatcgga gttgcgttt cagtgttgt gtgctttct gtgagaaat ccaccaaaa atgttgcca ttagcgact tttttttgt gtgttattt ctatcaaat ggacttttt agcgatgtc tcgggcctt caagacagt agcttaaac agcggggct gtgtcccac ttcgaacct caggtagca atgctttgt gatgcgggc ccccatggc caagaggca tatcatcca aagtatatg acagagcaa ggggaatgg ctctcagat ctctactcc aaagccacg tgttttaag gacaaaatg gatattttg aactattgc aaaaaggtg taccccaag cgagatatc acaaacatc gtcgaaagc agtcattat ttgaagatt gcctcctgg tgcaaagcc ggctccaca cctggggca caggctagg ggcaagtgc aagactgcc agatgggtc aaaccgttc cggtgtcta gagggtccg ttccagtcg gacgctctc ctagtgccc gaaaactgc ttattcgac cacatccac aaccagagc aactgctgg acattcggt cgctggaat gccactgca ggcaacgct tgccaagaa cgaggactc caacttcga tcgtttgct atgcttctt ccctgtggc atttctttg tttagtggt gtggaattt gtatgctgt cccaaacac ttcaaagac aacatgaag ccgaaaaag ccactggac ttaacaatc ctgaaaaag gaccaggac aacatcttc gacaccatc gacgacgat gactccact tcggacgaa gacgacgac aaggacgac ctggacgac ctcgccgac gacaccgag ctcgccgac gaccccaac atcgacgac gaggacgac gatgacgac gaatacgac gactcggat tgggacacc accccgtcg agcacgacg tccaaacaa tccaccacc acgaccacc accaccacc acggcgaag acgacgtca gtgccgacc ccagacccc tatttcacg catttcgat ccgagaaac gagcaccag agttacaag gaggcgcag gaacgtttg gaggagaca cacagggag aaagttaca agggttatg aaggattgg tcggacctg gaggagagg tatcaggac atgagacag acggatcca cgtggagct gatgagttc aagcagagg atgacgcag aggtttcaa cagacggtg caagcgctt gaagaagag ggcgatgct gagaaacat cagttggca gcaatgcat cagcaaaga gtcctagca catattaat caaaggaag aaggaggcc atgacttgt tatacacaa gcgttgaat gatgcgcct ctcaatacc catcgtgtg cagaaatgc ctggaaaaa ctgctccgt tctcttcac aaagaccga gctcatgct attaaccac taccgccat ctgttggca agtagtttg gaggcggca gctcgtgaa caaccgcgc attctcgaa cgtttaaca gacattgat cgtacggtt aatcaaagc atgcaaatg ttaaaaaga tatccagaa ttggcgacg aaaatcggt caattaatg gatgattac atccaagct ttgagatca aaagatgaa acacctgga tcacttcta gcgatgaca actgaagct gaagctgcg attttggat aaatataaa tcagaagtt acaaataaa caagcagag agagaacga caaaggttg caagagaag gagaggaag gaacagagg aagaaagaa cggtcggaa ttaagggag gagaagaag agagttgag gctgtttta gggaagaag attagcaat tttgatgat gaagacatg gaagatgag tcgttgcca gaagaaagg acaacaata atttcgacc accgggagc tcaactttc gaccttgtg agcaactcc atccctacc acaagccac cgcgacgcc gtggacgac gccgccgtc cagcaagcc gtcgaagac gtcgccgcc gtggtccac aaacaagac gtgatccga gtcgcccat gcaatggcg catgacatc agccacggc gaacctact tattcagtc cgacgtgag gtgtacggc agcaaagac ggcaaaagt gtttacttc actctcgcc ttcgccggc atggcgctg atggccgcc gtggtggtg ggggtgggg gtggcacgg cgcaggtcc gcacgctcc ccccaaagc caaggcttc gtggaggtg gaccaggcc gccaccccc gaggagcgg cacgtggcc aatatgcaa atcaatggc tacgaaaat cccacctac aaatatttc gaagtcaag gagtaataa acgatttgg ttgcgtttg gttggttag gttggtatt ttttacgtc attccattc gttttatta cagtgtact taactaagc aactgataa taataattc ggacatatg caaaaacgt attttccaa gtctaaaaa ttatgaaat aagtaataa tttttgcat atgacagcc agtccatgt tttacactt attgtataa tcttaaggc gagctgtgg agttacatt gccttggtg taattttac agtggaaga gtgtagcta gatgtatgg taccctcca taatatgtg acagcgttt ctctcttaa ggcctacgt ttcaaactt tcaataatg cagctagag attgaactc aaaatgact gagctacta gaaattggc gccaatttt aaattattt atgacgtat ggcaatttt ctggcaaaa ttttcgttt tcctaactt gaaaaattg tcaaaaagc cgtcaggtt ggcattacc ataagtaat tttttgttg ttaaagttg gtacttttt tttactgta ctacagtga cctgatttt tttgtacaa aatacaaaa tacgaaaat aactttaaa aattacaga catgttttt cgtatagtc tgtcatact tttgacaat tgtcaaaac atgactgat cagtttctt tttgataat taaatgttt gaagagcct tctagaatt tacaaaact gtcaactgt caaaattca aggctagta tgttttttt aaaatggga cggccatat tttttaacg gcaatagaa ctaaagttg acatttatg gtaattttt atcatcacg ttctatacc tatctcagt ttttgaaaa aaatcacga gaaacggat tttttatat cattactat aatcatttt ttgattatc tttttaaaa taataaaaa aaatcatag tagctttgc aatttgaca gttgacaag tttaaaaat tttggaaga ttgtttaat cgtttggta acaaatcca agaagaatt gtgatgttc tgaatgaga aaaaaattg atatatgtc aaaactctg acagatatg tgcaaagtt ggcgccatt ttggatcaa atttattgg ttggatcag tgtgtgtgc aaaataata agtttaaag tttgtgatg taggcccta gaagagtcg ccctgtact taattagaa tataacaag tgcatgttc aaccaacaa gctttttgt acaaagtct ttttacttt ttaccagct tgcaatcac ccgaatttg gcacaatcc gaacacttg tatgttttt tgtttgaga caaaacgaa agaaatgca agtgttcat cacaacgag acgtatttt cacgttgtc gaaagcttg atgaacaat tgtagataa cacaaatca tagaatttg tacaaaagt tgttgcgta cattctttt ttttaaaca ttgcgcgac aatgaggct tctgacagc ttaatgtgt acagtctag aatacaaaa aaaaaaaca accaacaac tttgtctta ctttctccc gtcttgtag gttttcaaa aactagata tgcgaatgt aaaattgat aaaagcgtg tttgatgtg catatagtt taatactcg attagaatt atttttcaa aacctgttt catataaga attattgta ttatatttc ttttctacg cgttttatt agtacgttt ctcctcatc ctgaagtaa ctagactag tttagcata cttgatagt taattatat gttttgtgg ccatgtaat tttaaattt aatgtaata aagaactaa attatta
