| General information | |||
| TC007714 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007419.2: 88685..97947 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC007714 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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|---|---|---|
| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02748 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_10755 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC007714 RA
MVLLLCKIC DRKIEFSQW KKHISNESH VRSLPSSIF IKGTPLNTK LNAVCEHFA KFGKLTKRE SLDFGTTLE ISYEDPNIY SDVVHREHA VEGEILKIF WYKTVKSFK VVVSFTKQE AITNGATRR IVELAKLLK DAQSFEQEL EIFLDTVKS YNDDFGRKS QVIRGLLLS HFNKVVFYG SSILDVDVF TRNCNLYLE TGKPILPIL IGSNRFSNS MLTSEGAKC IYEDSEIRV ACCVNLHKL LFVKTCDLV KYYLTLDEK DYTSSYMLY LMVIFYLQQ EPFKLPAVL SLQEDSFTN FVDGWNCYF FPKVVTSPA LENATLLKL LEGFFEFYS HFDYFTYVI CPYLGSPLK KTVFLDSCE LPESFDLYT KSSEKLQVN SGLCIQDPF NHSSNISKA MTDEMCGKL MTLCRVTSN LIKSGETRL YNLWMNNMH NLFEQITIE QDCEMDEKL WTQTVKDVL YQILTQVLF LDVKPGPNC THLPDCYVY SCTNTKSNT ESFVGKVTV KLAFNYGPR NKASVILKT NNPNFHLAH LIFIKLTSF LKEKIENLK NVTNKPNVK QVLISHLRS GTNLLHQLL ILLKNLPGK NDHSIKILQ DVKLMLTSQ YPKIKIKIF GSRVMKLGT CFSHLDFSL SKIASSDMT LLGELLSKF NIFTDVSIV NNGATLQCT HKATQTICH LWFVNHLWL ANERLVRWY FSSGVVVRQ MYMVVKFWT ICNDLDNHF TSYVLYLLV VFFLQQNHK FPSVATLQK DVPPYRINK WNCNFKQIE FLSNKCVKL SELLAGFFQ FYSNFDYIS YVVSPYFGA PLHKLDSNE TFKIHSGLC VQDFFVLSS NVTASIPLN IAGIFANLC QKTHRVLSL STPQLHTVL SRVGTDCSF FIEMEQGRN ELAWQQSVK EHTLKIISK VLGCKNINI IIVSSTYIK YMFVEMKHF WDKRADFMK SLQQQLNGC SDIERDITI TAHITKVSP SDADWKSSL ELKFHQNPT KVQITVIGN YNQTHTCYF FFTRLLKLL GEKPEISRN IQIPLLGND NTVAEWTGN YAELYRPIT ERIEGDFSE QIATFQKFQ TEVTKNVDI ITRDIGSVF TNPTTIGVF LPGDILDFF VDCDKESDM VILLSKSSE FRDVTLIQK CQNLVVTCT HRKTGIVCH LDNRHKVAH ATSHMIQAY LHEKQVLLF FNIIKLWAG RCKFMDECG FTSYIFNLM IIFFLQHEL HYPAVADLQ KSVNWGEIL LKKSLHELL VAFFKFYGD FDYISFVVA PFCGVAVEK SMLQDKKFP VNSGLCIQD LIDHSCNVS AEVTLNSSG KFAEMCRRA YTCMTDNQD LVPLVNPPI CNFAAFKIE ETGHKNEKE WIQLLRISV TKILVDMLG CKVSSVGNY NTFCVVYNC VLNHDIWSE RMSALKQLN GSDTLSKET SATMFIKEK YGATSLSKF VVNMSFDKD PISVQIEIR GTGDIFHLT HFVYTRLKL ILDNNIEDD ASPTDEVDL NQELLDDAC SKESDEMFE KLVASLQEA STIDEQMAI FSKILHYSK IEIEEIRDL IQIDLYSAL SPTFPSHKI EFFGSNIGF KNSDFDINI DNITSDIPT LELVKTRVM ACSNFSNVT MGDKQIKFK HDETDANCC LYVKNKMWV STSNLINYY LSLDNKLCD VFSVINFWA DYYGLKNGF FTTYSLNLL ILFYLEQEP YKLPRVKDL QKDVTPQIV DGWNCAFNC LKCDSLPLQ NASIAHLVI GFFHYYANF DYVSFVISP YFGTVIEKI RFVNQHFVV NCGVAIQNI FELSKNVSS DVPLGCVGR FAALCRNSV EALTKDLKL THLLEAKAD CIFEIGNLH KYDVEAFGI LVKRLILRV LNILNAKVI EKSYFRYEC EVKKSVFDN VKNISSPIH ATNDIIRDI IVTTFSEGL IQFSLTIDA TKNVCKIAL LGEENDLIY FMTHFIYIK LLKYLTEKP DQVSCGTKP SVLSSNFQT NDDEQNDSF EDDDEDIPS DFFDDFSKE EFLNGLDLE FCDEKRPKR RIRCSSSSS ESSTPPRKK KSISPHHDV NAQPTEAPQ PVDNIDQLF VERKISLTD FLTLTLRRE VKTNSEEKM KLISRCQEA IKILSEDLL IFPSKFTVT PSNKVVDSN KLRIKYRSP LLRNLVKFN FTTYQGPKK LDTFSQSLD HVLQRLGLL KEDNNLENL IDSITTALI NSPIVDKET VASILKASL LNLNCVNCD SKLMIQHVD VATQCRFEC ERNGFDEDS GTFFV
- >TC007714 RA
atggtttta cttttgtgc aaaatttgt gaccggaaa atcgaattt tcccagtgg aaaaaacac atttcgaat gaatcacac gttcgaagt ttaccatca tcaattttt attaaaggg actccactg aacaccaaa ttgaacgct gtctgcgaa cattttgca aaattcggg aaattaacc aaacgagaa tcgctagat ttcggcaca acccttgaa atatcatac gaggaccca aacatctat tctgacgtc gtgcaccgt gaacatgca gttgaaggc gaaatttta aaaattttc tggtacaaa acagtcaaa tcattcaaa gttgttgtc agcttcacc aaacaggag gcgattacc aatggcgcc acaagacgc atagtcgag ctggcaaaa ttgctgaaa gacgcacaa tcgtttgag caggaacta gaaattttt ctggacact gtcaagtct tataacgat gatttcggg agaaaaagt caagtgatt cgtggtttg ttgctgtcg catttcaac aaagtagtt ttctatgga tcgagtatc ctggatgtg gatgtgttt acgagaaat tgcaatctt tacctcgag actggtaag ccaattttg ccgattttg attggttcg aatcggttc agcaacagc atgttgaca agtgaaggt gcgaaatgt atttacgaa gatagtgag attcgagtg gcttgttgc gtgaatttg cacaagctt ttgtttgtc aaaacttgc gatttggtt aagtattac ttaacgttg gacgaaaaa gattacacg tccagctat atgctgtat cttatggtt attttctat ttgcaacaa gagccgttc aagttacct gccgtgttg tcactacaa gaggattca tttactaat tttgtagac ggttggaat tgttatttt tttccaaaa gttgtgaca agtccagct cttgaaaac gccactctt ctcaaattg cttgagggt tttttcgaa ttttattca cactttgat tacttcact tatgtgata tgtccatat ttgggatca ccgttgaaa aaaacagtt tttttggac tcttgcgaa ttgcctgaa tcgtttgat ctttacaca aaatcgtca gaaaaacta caagtcaat tcaggattg tgtattcaa gatcctttc aatcattcg agcaacatt tcaaaggca atgactgat gaaatgtgc ggaaaactg atgactttg tgcagagtc acttcaaac ttgatcaaa tcgggagaa acaagactt tacaattta tggatgaac aatatgcat aatctcttt gagcaaata acaattgag caagactgc gaaatggac gagaaatta tggactcaa acagtcaaa gacgttctt tatcaaatc ttgacgcaa gttttgttt ttggatgtt aaaccaggc ccgaattgt acacattta cccgactgt tatgtgtat tcatgcaca aacaccaaa tcaaatacg gaaagtttt gttggaaag gttacagtc aagctagcg tttaattat ggacctcgc aataaggct tcagtcatc cttaagaca aataatccc aattttcac ctagcacac ttgattttc atcaaactg acgagtttt ctaaaagaa aagattgaa aacttgaaa aatgtcact aataaacca aatgtcaaa caagtgctg ataagccac ctgagatca ggcacgaac ctccttcac cagttgcta attttattg aaaaatctt ccggggaaa aatgaccac agcataaag attcttcag gatgtgaaa ttaatgtta acatcgcaa tatcctaaa attaaaatt aaaattttt ggttcgaga gtaatgaaa ctgggcact tgtttcagt cacttggac tttagttta agtaaaatt gcatcaagt gacatgact ttgctaggc gaactgttg tcgaaattt aacattttt actgacgtt tcaattgtt aacaacgga gcaacgctt caatgcaca cataaagcc actcagacc atttgccac ttatggttt gtaaaccat ttgtggttg gctaatgaa cggttggtg aggtggtac ttcagttcg ggagtggtt gtacgtcag atgtatatg gttgttaag ttttggacg atttgtaat gaccttgat aaccatttt acctcgtat gttctctac ttgttggtg gtttttttc ctccaacaa aaccacaag tttccctct gttgcaaca ctgcaaaag gatgttcct ccttatcgg atcaataaa tggaattgc aatttcaaa caaatcgaa tttcttagc aataaatgt gtgaaatta tccgaactt ctagccggt tttttccaa ttttatagc aactttgac tacatttcg tacgtggtt tcgccatat tttggcgcc cctttgcac aaacttgac tcaaatgaa acatttaaa atacactcc ggcctatgt gtgcaggat tttttcgtt ctatcatca aacgtcaca gcttcgatt cctctaaat atcgctggg atttttgca aatttgtgc caaaaaact cacagggtg ttatcatta tcaactccc caattacac acggtgttg tcacgtgtt ggcaccgat tgtagtttt tttattgaa atggagcaa ggacgcaat gaattggcc tggcaacaa tcggtcaaa gagcacact ttaaaaatt atatccaaa gtgttgggt tgcaaaaat ataaacata atcattgtc tcctccaca tatatcaag tatatgttc gtcgaaatg aaacatttt tgggacaag agagccgat tttatgaaa agtttgcaa caacaattg aacggttgt agtgatatt gagcgagat attacaatt actgcacat atcaccaaa gtatcacca agtgatgcg gactggaag tcatcactt gaattaaaa tttcaccaa aatccaaca aaagtacaa attacagtc ataggaaat tacaatcag acacacacc tgttatttt ttcttcacc agactgctc aaattactg ggggaaaaa ccggagatc agtagaaac atacaaata ccattgctt ggcaatgat aacacagtg gctgagtgg accggtaac tacgccgaa ttatatcga ccgataacc gaacgtatt gaaggagat ttttctgaa caaatagca acatttcag aagtttcaa accgaagtg accaaaaac gtcgatata attacacgt gacataggt tcggttttt acaaatcca accaccatt ggtgtgttt ctccctggt gatattttg gattttttt gttgactgt gataaggaa agtgatatg gtaatcctg cttagtaaa agtagtgaa tttcgtgat gtgacactt atccaaaag tgtcaaaac cttgtggta acttgcacg catcgtaaa accggaatt gtttgtcac ttggataac cgccacaag gtggcgcat gcgactagt cacatgatc caagcctac ttacacgaa aaacaagtc ctgttattt ttcaatata ataaaattg tgggctgga cgttgcaaa ttcatggat gagtgcggt tttacgagt tatatattc aatttgatg ataattttt ttcttgcaa cacgaattg cattatcca gcagttgcc gacttacag aaatctgta aactggggt gaaattctt ctgaaaaag agtttacac gagttgcta gtggcgttt tttaagttt tacggcgat tttgattat atttcgttt gtcgttgcg ccgttttgc ggtgttgcg gttgaaaaa tcaatgttg caagataag aaatttcct gtgaattca ggattatgt attcaggat ctgatcgat cactcgtgt aacgtttcg gctgaggtt acgttaaac tcatcaggg aaattcgca gaaatgtgt cggagagcg tatacttgt atgacagat aatcaggat ttagtacct ttggtgaat ccccccatt tgtaatttt gcggcgttt aaaatcgag gaaactggc cataaaaac gaaaaagaa tggatacaa ttacttagg atcagtgtt actaaaatt ttggttgat atgttagga tgtaaagta tcttccgtt ggaaattat aacactttt tgtgttgtt tacaactgc gttttgaat catgatatt tggagtgaa agaatgtcc gctttgaaa caattaaac ggttccgat actttgagt aaagaaact tcagctacg atgtttatt aaggaaaaa tatggagcc acttctttg tccaaattc gttgttaat atgtcgttt gacaaagat ccgatttct gttcaaatt gaaattaga ggaactgga gatattttt catttgact cattttgtc tacacaaga ttgaaacta attctcgac aacaatata gaggatgac gcttctccc acagatgaa gtagatttg aaccaagaa ttgttggat gacgcctgc agtaaggaa tcggatgaa atgttcgaa aaattggta gcaagtctg caggaagca tcaactatc gacgagcaa atggcgatt ttttcgaaa attcttcac tattcgaaa attgaaatc gaagagatt cgagatttg atccaaatc gatttgtat tcagcgtta agtccgact tttccgtct cataagatc gaatttttc ggttcaaat atcggtttt aaaaacagt gattttgac ataaacatc gataatatc actagcgac ataccaacc ctcgaacta gtgaaaacc cgagttatg gcatgttct aacttttca aatgtcaca atgggcgac aaacaaatc aagttcaaa catgacgaa accgacgcc aactgttgt ctgtatgta aaaaacaaa atgtgggtc agcaccagc aatttgatc aactattac ttaagtttg gataacaag ttatgtgat gttttttct gtgattaat ttttgggcc gattattac ggcttaaaa aatggtttt ttcaccact tattcatta aacctactc atacttttc taccttgaa caagaaccg tacaagtta ccacgtgtc aaagacttg caaaaagat gtaacacct cagattgta gatggctgg aactgtgcg ttcaattgt ttgaaatgt gacagtttg ccactacaa aacgcctca attgcccat ctagtcatt gggtttttc cactattat gccaacttt gattacgtt tcgtttgtc atttcgccg tattttgga accgtgatt gaaaaaatt cgttttgtt aatcaacat ttcgtagtt aattgtggt gttgccatt cagaatatt ttcgaactt tcgaagaat gtctcatcg gatgtgcca ctaggatgt gttggaagg tttgcagcc ctgtgtcgg aactcagtt gaagcatta accaaagat ttgaaattg actcattta ttggaagcg aaggctgat tgtattttt gaaattgga aatttacat aaatacgac gttgaagcg tttggaatt ttggtcaag agactaatt ttgagagtt ttgaacatt ttgaacgca aaagtgatc gaaaaatcg tactttcgg tacgagtgt gaggtcaag aagagtgtc tttgataat gtgaagaat atttcttca cccattcat gctaccaat gatattatt cgagacatt attgttacg acgttttcc gaaggcttg atccaattt tcactcaca atcgatgct acgaaaaat gtttgtaag attgccttg ttgggggaa gaaaacgat ttgatctat ttcatgaca cactttatt tatatcaaa cttttgaaa tatctgaca gagaaacca gatcaagtg tcctgtggc acgaaacca tctgtcctt tcttcaaat tttcaaaca aatgatgat gaacaaaac gactcattt gaagatgat gatgaagac attccatct gactttttc gacgatttc tcaaaagaa gaatttctt aacggtctt gacttagag ttttgtgat gaaaaacgc ccaaagcgt agaattcgc tgttctagt tctagttct gaaagttcg acaccacca agaaaaaaa aagtcaatt tcacctcat catgatgtt aatgcacag cctactgag gcccctcaa cctgtcgac aacatagat caacttttt gtcgagagg aagatttca ttgacggat ttcttaaca ctgacctta cgacgtgaa gtaaaaacc aatagtgaa gaaaaaatg aaattgatt tcgcgatgt caagaggct attaaaatc ctaagtgaa gatttgttg atttttcca agcaagttt acagttaca ccatcgaat aaagttgtc gattctaat aagttaaga attaaatat cgatcgccg ttacttcga aatttggtt aaatttaat tttacgact tatcaaggt ccaaaaaaa ctcgatacg ttttcacag agtttagat catgttttg caacgcctg ggtttgttg aaagaagat aataatttg gagaacttg atcgattca ataactaca gctttgatt aatagtccg attgtggat aaagaaact gtggcttcg attttgaaa gcttcactt ttgaatttg aattgtgta aattgtgat tcgaaatta atgatacaa catgtggat gtagcaacg cagtgtcgg tttgaatgt gaaagaaat ggatttgat gaggattca gggactttc tttgtatga
- >TC007714 RA
tcaaaacca gcaaaaaat gacgtcaga gtgtcggta ataccaaaa ttcggatta aaataaatt gggtgtgcc tttagacac actttgccg gcacatttg cgcgttcga tctggtcaa attatcaaa tcaattaat tttttttcc aaactcttg tgtcatatc ttttgtaca aaacacgtt gcgtcatcg tacaaatct agtgtaatt atgatggtt tagtattta ttttttccg agttattta tttgtcgat aaaatgtga cgtttggtt gtaacgcca aaaaacggc tgacacttg aacagaata taaacaatt ttggtatga ggtgattaa aatgtgaga aaaaaagga ataagaagc gaatagttt tgcatatta aaatgaaac gatttttga ctttctaac cctgaaaat ggttttact tttgtgcaa aatttgtga ccggaaaat cgaattttc ccagtggaa aaaacacat ttcgaatga atcacacgt tcgaagttt accatcatc aatttttat taaagggac tccactgaa caccaaatt gaacgctgt ctgcgaaca ttttgcaaa attcgggaa attaaccaa acgagaatc gctagattt cggcacaac ccttgaaat atcatacga ggacccaaa catctattc tgacgtcgt gcaccgtga acatgcagt tgaaggcga aattttaaa aattttctg gtacaaaac agtcaaatc attcaaagt tgttgtcag cttcaccaa acaggaggc gattaccaa tggcgccac aagacgcat agtcgagct ggcaaaatt gctgaaaga cgcacaatc gtttgagca ggaactaga aatttttct ggacactgt caagtctta taacgatga tttcgggag aaaaagtca agtgattcg tggtttgtt gctgtcgca tttcaacaa agtagtttt ctatggatc gagtatcct ggatgtgga tgtgtttac gagaaattg caatcttta cctcgagac tggtaagcc aattttgcc gattttgat tggttcgaa tcggttcag caacagcat gttgacaag tgaaggtgc gaaatgtat ttacgaaga tagtgagat tcgagtggc ttgttgcgt gaatttgca caagctttt gtttgtcaa aacttgcga tttggttaa gtattactt aacgttgga cgaaaaaga ttacacgtc cagctatat gctgtatct tatggttat tttctattt gcaacaaga gccgttcaa gttacctgc cgtgttgtc actacaaga ggattcatt tactaattt tgtagacgg ttggaattg ttatttttt tccaaaagt tgtgacaag tccagctct tgaaaacgc cactcttct caaattgct tgagggttt tttcgaatt ttattcaca ctttgatta cttcactta tgtgatatg tccatattt gggatcacc gttgaaaaa aacagtttt tttggactc ttgcgaatt gcctgaatc gtttgatct ttacacaaa atcgtcaga aaaactaca agtcaattc aggattgtg tattcaaga tcctttcaa tcattcgag caacatttc aaaggcaat gactgatga aatgtgcgg aaaactgat gactttgtg cagagtcac ttcaaactt gatcaaatc gggagaaac aagacttta caatttatg gatgaacaa tatgcataa tctctttga gcaaataac aattgagca agactgcga aatggacga gaaattatg gactcaaac agtcaaaga cgttcttta tcaaatctt gacgcaagt tttgttttt ggatgttaa accaggccc gaattgtac acatttacc cgactgtta tgtgtattc atgcacaaa caccaaatc aaatacgga aagttttgt tggaaaggt tacagtcaa gctagcgtt taattatgg acctcgcaa taaggcttc agtcatcct taagacaaa taatcccaa ttttcacct agcacactt gattttcat caaactgac gagttttct aaaagaaaa gattgaaaa cttgaaaaa tgtcactaa taaaccaaa tgtcaaaca agtgctgat aagccacct gagatcagg cacgaacct ccttcacca gttgctaat tttattgaa aaatcttcc ggggaaaaa tgaccacag cataaagat tcttcagga tgtgaaatt aatgttaac atcgcaata tcctaaaat taaaattaa aatttttgg ttcgagagt aatgaaact gggcacttg tttcagtca cttggactt tagtttaag taaaattgc atcaagtga catgacttt gctaggcga actgttgtc gaaatttaa catttttac tgacgtttc aattgttaa caacggagc aacgcttca atgcacaca taaagccac tcagaccat ttgccactt atggtttgt aaaccattt gtggttggc taatgaacg gttggtgag gtggtactt cagttcggg agtggttgt acgtcagat gtatatggt tgttaagtt ttggacgat ttgtaatga ccttgataa ccattttac ctcgtatgt tctctactt gttggtggt ttttttcct ccaacaaaa ccacaagtt tccctctgt tgcaacact gcaaaagga tgttcctcc ttatcggat caataaatg gaattgcaa tttcaaaca aatcgaatt tcttagcaa taaatgtgt gaaattatc cgaacttct agccggttt tttccaatt ttatagcaa ctttgacta catttcgta cgtggtttc gccatattt tggcgcccc tttgcacaa acttgactc aaatgaaac atttaaaat acactccgg cctatgtgt gcaggattt tttcgttct atcatcaaa cgtcacagc ttcgattcc tctaaatat cgctgggat ttttgcaaa tttgtgcca aaaaactca cagggtgtt atcattatc aactcccca attacacac ggtgttgtc acgtgttgg caccgattg tagtttttt tattgaaat ggagcaagg acgcaatga attggcctg gcaacaatc ggtcaaaga gcacacttt aaaaattat atccaaagt gttgggttg caaaaatat aaacataat cattgtctc ctccacata tatcaagta tatgttcgt cgaaatgaa acatttttg ggacaagag agccgattt tatgaaaag tttgcaaca acaattgaa cggttgtag tgatattga gcgagatat tacaattac tgcacatat caccaaagt atcaccaag tgatgcgga ctggaagtc atcacttga attaaaatt tcaccaaaa tccaacaaa agtacaaat tacagtcat aggaaatta caatcagac acacacctg ttatttttt cttcaccag actgctcaa attactggg ggaaaaacc ggagatcag tagaaacat acaaatacc attgcttgg caatgataa cacagtggc tgagtggac cggtaacta cgccgaatt atatcgacc gataaccga acgtattga aggagattt ttctgaaca aatagcaac atttcagaa gtttcaaac cgaagtgac caaaaacgt cgatataat tacacgtga cataggttc ggtttttac aaatccaac caccattgg tgtgtttct ccctggtga tattttgga tttttttgt tgactgtga taaggaaag tgatatggt aatcctgct tagtaaaag tagtgaatt tcgtgatgt gacacttat ccaaaagtg tcaaaacct tgtggtaac ttgcacgca tcgtaaaac cggaattgt ttgtcactt ggataaccg ccacaaggt ggcgcatgc gactagtca catgatcca agcctactt acacgaaaa acaagtcct gttattttt caatataat aaaattgtg ggctggacg ttgcaaatt catggatga gtgcggttt tacgagtta tatattcaa tttgatgat aattttttt cttgcaaca cgaattgca ttatccagc agttgccga cttacagaa atctgtaaa ctggggtga aattcttct gaaaaagag tttacacga gttgctagt ggcgttttt taagtttta cggcgattt tgattatat ttcgtttgt cgttgcgcc gttttgcgg tgttgcggt tgaaaaatc aatgttgca agataagaa atttcctgt gaattcagg attatgtat tcaggatct gatcgatca ctcgtgtaa cgtttcggc tgaggttac gttaaactc atcagggaa attcgcaga aatgtgtcg gagagcgta tacttgtat gacagataa tcaggattt agtaccttt ggtgaatcc ccccatttg taattttgc ggcgtttaa aatcgagga aactggcca taaaaacga aaaagaatg gatacaatt acttaggat cagtgttac taaaatttt ggttgatat gttaggatg taaagtatc ttccgttgg aaattataa cactttttg tgttgttta caactgcgt tttgaatca tgatatttg gagtgaaag aatgtccgc tttgaaaca attaaacgg ttccgatac tttgagtaa agaaacttc agctacgat gtttattaa ggaaaaata tggagccac ttctttgtc caaattcgt tgttaatat gtcgtttga caaagatcc gatttctgt tcaaattga aattagagg aactggaga tatttttca tttgactca ttttgtcta cacaagatt gaaactaat tctcgacaa caatataga ggatgacgc ttctcccac agatgaagt agatttgaa ccaagaatt gttggatga cgcctgcag taaggaatc ggatgaaat gttcgaaaa attggtagc aagtctgca ggaagcatc aactatcga cgagcaaat ggcgatttt ttcgaaaat tcttcacta ttcgaaaat tgaaatcga agagattcg agatttgat ccaaatcga tttgtattc agcgttaag tccgacttt tccgtctca taagatcga atttttcgg ttcaaatat cggttttaa aaacagtga ttttgacat aaacatcga taatatcac tagcgacat accaaccct cgaactagt gaaaacccg agttatggc atgttctaa cttttcaaa tgtcacaat gggcgacaa acaaatcaa gttcaaaca tgacgaaac cgacgccaa ctgttgtct gtatgtaaa aaacaaaat gtgggtcag caccagcaa tttgatcaa ctattactt aagtttgga taacaagtt atgtgatgt tttttctgt gattaattt ttgggccga ttattacgg cttaaaaaa tggtttttt caccactta ttcattaaa cctactcat acttttcta ccttgaaca agaaccgta caagttacc acgtgtcaa agacttgca aaaagatgt aacacctca gattgtaga tggctggaa ctgtgcgtt caattgttt gaaatgtga cagtttgcc actacaaaa cgcctcaat tgcccatct agtcattgg gtttttcca ctattatgc caactttga ttacgtttc gtttgtcat ttcgccgta ttttggaac cgtgattga aaaaattcg ttttgttaa tcaacattt cgtagttaa ttgtggtgt tgccattca gaatatttt cgaactttc gaagaatgt ctcatcgga tgtgccact aggatgtgt tggaaggtt tgcagccct gtgtcggaa ctcagttga agcattaac caaagattt gaaattgac tcatttatt ggaagcgaa ggctgattg tatttttga aattggaaa tttacataa atacgacgt tgaagcgtt tggaatttt ggtcaagag actaatttt gagagtttt gaacatttt gaacgcaaa agtgatcga aaaatcgta ctttcggta cgagtgtga ggtcaagaa gagtgtctt tgataatgt gaagaatat ttcttcacc cattcatgc taccaatga tattattcg agacattat tgttacgac gttttccga aggcttgat ccaattttc actcacaat cgatgctac gaaaaatgt ttgtaagat tgccttgtt gggggaaga aaacgattt gatctattt catgacaca ctttattta tatcaaact tttgaaata tctgacaga gaaaccaga tcaagtgtc ctgtggcac gaaaccatc tgtcctttc ttcaaattt tcaaacaaa tgatgatga acaaaacga ctcatttga agatgatga tgaagacat tccatctga ctttttcga cgatttctc aaaagaaga atttcttaa cggtcttga cttagagtt ttgtgatga aaaacgccc aaagcgtag aattcgctg ttctagttc tagttctga aagttcgac accaccaag aaaaaaaaa gtcaatttc acctcatca tgatgttaa tgcacagcc tactgaggc ccctcaacc tgtcgacaa catagatca actttttgt cgagaggaa gatttcatt gacggattt cttaacact gaccttacg acgtgaagt aaaaaccaa tagtgaaga aaaaatgaa attgatttc gcgatgtca agaggctat taaaatcct aagtgaaga tttgttgat ttttccaag caagtttac agttacacc atcgaataa agttgtcga ttctaataa gttaagaat taaatatcg atcgccgtt acttcgaaa tttggttaa atttaattt tacgactta tcaaggtcc aaaaaaact cgatacgtt ttcacagag tttagatca tgttttgca acgcctggg tttgttgaa agaagataa taatttgga gaacttgat cgattcaat aactacagc tttgattaa tagtccgat tgtggataa agaaactgt ggcttcgat tttgaaagc ttcactttt gaatttgaa ttgtgtaaa ttgtgattc gaaattaat gatacaaca tgtggatgt agcaacgca gtgtcggtt tgaatgtga aagaaatgg atttgatga ggattcagg gactttctt tgtatgagg agaagagaa tttcatttg atttttttt tttaataag aagttaaaa tagtttctt aagagtatt acattagtt ttaagaaaa agtttattt ttgttactt atttgaaac gttattaaa cgtttttct cgatgcttt tgttttatt gcgtaacga cttaaattt gggtattta gtttttttg aaatttccc aaataatag gacgcgatc ctgtaagtt ttttttgtg taaatgtct acaaacgac gtgtttttg agtaagaca ttatcatgg cttctgaga cactctgca gccaccgaa ggcctaagt ctaacccct gagggcttc gcccccgtt tcggaagtc ctaaaccat cgccaactg cgtggtaaa ttcacagtg gaggacatc caacgcatt gttgaaagc aacaacaaa caacgattt ttcctacga caaaacggt gctattttg gaaataaga gccaatcaa ggccactcg atgcaagtg actgagtta gagttggcg tcttatttg aatgatcca aatgtggat gttgttcat ggaacgttt tttcgaaat ttggatgct ataaagaga ataggtttg agtcgcatg tccagaaat cacattcat ttcactcgg gacttggct ctgattcga aaaaacgtc caagtttta atttacatt gatgttgca aaggcgttg agtgacggt atcaagttt tacatgtcg tcaaatggt gttatttta agtcccggc aacgaaaag ggtattttg gaacccaaa tacttctgg aaaattgtg aaaagataa ctgcaataa attttaatt aaagtt
