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Gene information
General information
TC032714
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC007755
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid33: 476370..482900
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032714
    iBeetle screen iB_02746
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032714 RA
      MFGSDCNNE RALSQVEKS FLLHAERGD CATVRRIIE 
      EYGGGEGDQ EFDINCIDA LNRSAFVIA IENGNIELV 
      KLLLESQID VKDGLLHAI NEEFVEAVE MLLDWEERT 
      HQVGDLYSW EKVERCSAT FTPDITPLV LAGHKNNYE 
      IIKLLLDRG ATLPLPHDV KCGCDECVC SNQSDSLRH 
      SQARINSYK ALTSPSLIS LSSTDPLFT AFELSGDLR 
      RLSRLETEF RAEYNNMRT VVENFAALL LDHVKTSEE 
      LMILLNYDE KNPSWVLGE QQTLGRLKL AIKFKQKKF 
      VAHPNVQQI LGTIWYEGL PGFRRKNLF GQTLQLMRL 
      SAMFTVYCL VYMLLPTSK MGLFIKKPF VKFICHSSS 
      YILFLVLLG VTSQRFEIT ILEWVDTGW SRDFVDEWK 
      RKERGALLG FAECAVVLY VSSKMCKEI KHLWASNLL 
      EYVSDLWNI VDFVTNLFY VAWLSLRLC SIYLTRASR 
      AESLGEDPW FPREEWNSF EPMLIAEGA FAAAMIFSF 
      LKLVHIFSV NPHLGPLQI SLSRMIIDI VKFFFIYTL 
      VLFAFGCGL NQLLWYYAE LEKNKCYHR GGMPDFEAH 
      DKACTIWRR YANLFETSQ SLFWASFGL VDLLSFELT 
      GIKSFTRFW ALLMFGSYS VINIIVLLN MLIAMMSNS 
      FQIISERAD VEWKFARSK LWMTYFEDD DVLPPPFNI 
      LPNTELFCR VFKLRRKKN KICNKESQL RIKAKHDSV 
      VRLLLKRYN TSEQRKRHE FGVTEDDVI EIRQDISTL 
      RYEIIDILK NNGMRTPNM SFEDKQVVG KKGKMIERR 
      ILRDFHIGV VDEVGKSEV SYSPNKRKR RTFRKEKKD 
      WNSLVRKNT KSYDPIGSS KNHFE
    CDS sequence
    • >TC032714 RA
      atgtttgga tctgattgt aataatgaa agggcgtta 
      agtcaggtg gaaaaatcg tttttgctt catgccgaa 
      aggggtgat tgtgctaca gttcgcagg ataattgag 
      gaatatggt ggtggtgag ggcgatcaa gagtttgat 
      ataaactgc atcgatgct ctaaatcgc tcagcgttc 
      gttatagca attgagaat ggaaatatc gagctggtg 
      aagcttctc cttgagtcg cagattgac gttaaagat 
      ggattgctt catgcaata aatgaggaa tttgtggaa 
      gcggttgaa atgctttta gattgggag gagaggacg 
      catcaagtt ggcgatttg tacagttgg gaaaaagtc 
      gaacggtgt tcggccact tttacacct gatattaca 
      cctctggtc ttagccggg cataaaaat aattacgag 
      attattaag ctgttgctg gatagagga gccactctt 
      ccattgcct cacgatgtc aaatgtggt tgtgacgaa 
      tgcgtttgc tcaaaccaa agtgactct ttacgacat 
      tctcaagct cggatcaac tcctacaaa gctctaaca 
      tccccttca ctcatttcc ctctcatcc actgaccct 
      ctatttact gctttcgaa ctttcgggc gacttgaga 
      cgtctcagt cgactagaa accgaattt cgagccgaa 
      tataacaac atgcgaact gttgtggaa aatttcgca 
      gctttgctc ctagaccat gtaaaaacc tcagaagaa 
      ttaatgatt ctgttgaac tacgacgaa aaaaatcct 
      tcatgggtt ttgggcgaa caacaaact ttgggacgc 
      ttaaaactc gccataaaa tttaaacag aaaaaattc 
      gtggcacat cccaatgtc caacaaatc ttaggcaca 
      atctggtac gagggactc cccggattt cgccgaaaa 
      aacctcttc ggacaaacc ttacaacta atgagactt 
      agtgcaatg tttactgtt tattgcctg gtttatatg 
      ctactacca acgtccaaa atgggtctc ttcattaaa 
      aaacccttc gttaagttt atctgccac tcaagcagc 
      tatattctt tttttggtt ttgttgggc gtgacatcg 
      caacgtttc gaaatcaca attttggaa tgggttgat 
      acagggtgg tccagagat ttcgtcgat gaatggaag 
      aggaaagaa cgtggggct cttttagga tttgccgaa 
      tgtgcagtt gttctctac gtttcaagt aaaatgtgc 
      aaagagatc aagcacttg tgggcctcg aatttgctt 
      gaatacgtt tcggatttg tggaatatt gttgatttt 
      gtgacgaat cttttttac gtagcttgg ctttcttta 
      cgactttgt tccatctat ttaacacgg gcaagtaga 
      gctgagtct ctgggtgaa gatccttgg tttccacgg 
      gaggaatgg aactcattt gagccaatg ttgatagcc 
      gaaggggct tttgcagct gcgatgatt ttcagtttc 
      ctgaagctg gttcacata ttcagcgta aatccgcac 
      ttgggccca cttcaaatc tcactaagc cgcatgatt 
      attgacatc gtcaagttc ttctttatt tatactttg 
      gttttattt gcgttcggt tgtggtttg aaccaactg 
      ttgtggtat tatgccgaa cttgagaag aacaagtgt 
      taccacagg ggcggaatg cccgatttt gaggcccat 
      gacaaggct tgcacgatc tggaggaga tatgccaat 
      ttatttgaa acgtcccag tcacttttt tgggccagc 
      tttggtttg gttgacttg ctctcattt gaactaaca 
      gggatcaaa agtttcacc cggttttgg gccttgcta 
      atgtttggc tcgtactca gtcattaat atcatcgtt 
      ttgttgaat atgttgatc gcaatgatg tctaactcg 
      tttcaaatc atctcagaa cgtgctgac gttgaatgg 
      aaattcgcc agaagcaag ctgtggatg acgtacttc 
      gaagatgac gatgtgtta cctccacct ttcaatatt 
      ttgccaaac actgaactg ttttgtcgg gttttcaaa 
      ctgaggcgc aagaagaat aaaatttgc aataaagaa 
      tcacagctt cgtatcaaa gcaaagcac gattcggtc 
      gttcgtttg ctactaaaa cgctacaat acatcagaa 
      cagcgcaaa cgacacgaa tttggagtt actgaagac 
      gatgttatt gaaatcaga caagatatt tcgactttg 
      cggtacgaa atcatcgat attttgaaa aataacggc 
      atgcgaacg ccgaacatg tcgtttgag gataaacaa 
      gtggttggg aaaaaaggg aaaatgatt gagcgacga 
      attttgcgt gatttccac atcggagtg gtggacgaa 
      gttggcaaa agcgaagtc agttattcc ccgaataag 
      aggaaacgg cgcactttc agaaaggaa aaaaaggat 
      tggaattca ctagtgagg aagaacacc aaaagttac 
      gacccgatt ggttcttct aaaaatcat tttgaataa
    mRNA sequence
    • >TC032714 RA
      atgtttgga tctgattgt aataatgaa agggcgtta 
      agtcaggtg gaaaaatcg tttttgctt catgccgaa 
      aggggtgat tgtgctaca gttcgcagg ataattgag 
      gaatatggt ggtggtgag ggcgatcaa gagtttgat 
      ataaactgc atcgatgct ctaaatcgc tcagcgttc 
      gttatagca attgagaat ggaaatatc gagctggtg 
      aagcttctc cttgagtcg cagattgac gttaaagat 
      ggattgctt catgcaata aatgaggaa tttgtggaa 
      gcggttgaa atgctttta gattgggag gagaggacg 
      catcaagtt ggcgatttg tacagttgg gaaaaagtc 
      gaacggtgt tcggccact tttacacct gatattaca 
      cctctggtc ttagccggg cataaaaat aattacgag 
      attattaag ctgttgctg gatagagga gccactctt 
      ccattgcct cacgatgtc aaatgtggt tgtgacgaa 
      tgcgtttgc tcaaaccaa agtgactct ttacgacat 
      tctcaagct cggatcaac tcctacaaa gctctaaca 
      tccccttca ctcatttcc ctctcatcc actgaccct 
      ctatttact gctttcgaa ctttcgggc gacttgaga 
      cgtctcagt cgactagaa accgaattt cgagccgaa 
      tataacaac atgcgaact gttgtggaa aatttcgca 
      gctttgctc ctagaccat gtaaaaacc tcagaagaa 
      ttaatgatt ctgttgaac tacgacgaa aaaaatcct 
      tcatgggtt ttgggcgaa caacaaact ttgggacgc 
      ttaaaactc gccataaaa tttaaacag aaaaaattc 
      gtggcacat cccaatgtc caacaaatc ttaggcaca 
      atctggtac gagggactc cccggattt cgccgaaaa 
      aacctcttc ggacaaacc ttacaacta atgagactt 
      agtgcaatg tttactgtt tattgcctg gtttatatg 
      ctactacca acgtccaaa atgggtctc ttcattaaa 
      aaacccttc gttaagttt atctgccac tcaagcagc 
      tatattctt tttttggtt ttgttgggc gtgacatcg 
      caacgtttc gaaatcaca attttggaa tgggttgat 
      acagggtgg tccagagat ttcgtcgat gaatggaag 
      aggaaagaa cgtggggct cttttagga tttgccgaa 
      tgtgcagtt gttctctac gtttcaagt aaaatgtgc 
      aaagagatc aagcacttg tgggcctcg aatttgctt 
      gaatacgtt tcggatttg tggaatatt gttgatttt 
      gtgacgaat cttttttac gtagcttgg ctttcttta 
      cgactttgt tccatctat ttaacacgg gcaagtaga 
      gctgagtct ctgggtgaa gatccttgg tttccacgg 
      gaggaatgg aactcattt gagccaatg ttgatagcc 
      gaaggggct tttgcagct gcgatgatt ttcagtttc 
      ctgaagctg gttcacata ttcagcgta aatccgcac 
      ttgggccca cttcaaatc tcactaagc cgcatgatt 
      attgacatc gtcaagttc ttctttatt tatactttg 
      gttttattt gcgttcggt tgtggtttg aaccaactg 
      ttgtggtat tatgccgaa cttgagaag aacaagtgt 
      taccacagg ggcggaatg cccgatttt gaggcccat 
      gacaaggct tgcacgatc tggaggaga tatgccaat 
      ttatttgaa acgtcccag tcacttttt tgggccagc 
      tttggtttg gttgacttg ctctcattt gaactaaca 
      gggatcaaa agtttcacc cggttttgg gccttgcta 
      atgtttggc tcgtactca gtcattaat atcatcgtt 
      ttgttgaat atgttgatc gcaatgatg tctaactcg 
      tttcaaatc atctcagaa cgtgctgac gttgaatgg 
      aaattcgcc agaagcaag ctgtggatg acgtacttc 
      gaagatgac gatgtgtta cctccacct ttcaatatt 
      ttgccaaac actgaactg ttttgtcgg gttttcaaa 
      ctgaggcgc aagaagaat aaaatttgc aataaagaa 
      tcacagctt cgtatcaaa gcaaagcac gattcggtc 
      gttcgtttg ctactaaaa cgctacaat acatcagaa 
      cagcgcaaa cgacacgaa tttggagtt actgaagac 
      gatgttatt gaaatcaga caagatatt tcgactttg 
      cggtacgaa atcatcgat attttgaaa aataacggc 
      atgcgaacg ccgaacatg tcgtttgag gataaacaa 
      gtggttggg aaaaaaggg aaaatgatt gagcgacga 
      attttgcgt gatttccac atcggagtg gtggacgaa 
      gttggcaaa agcgaagtc agttattcc ccgaataag 
      aggaaacgg cgcactttc agaaaggaa aaaaaggat 
      tggaattca ctagtgagg aagaacacc aaaagttac 
      gacccgatt ggttcttct aaaaatcat tttgaataa