Status
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Gene information
General information
TC032667
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003979,TC003980
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 29337218..29443319
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032667
    iBeetle screen iB_02745

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_07095

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    2

    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032667 RA
      MSAYRNYVP WNKSPNSST SFSSKKEGT KYDSSFGKK 
      KDEEKSATA RIASKYGAY GSGYQRDSS ASGSSSYSS 
      RLKPTLEKK ETNHPPVSY RALARTTPR SRDPSPDKS 
      SSSSSYNYN RLYPTRPYT RSVSRELDT STTIPKYSG 
      RSSLSKDDV SSRRSSVSR EDLSKYTGR SSTTKEDLV 
      PKYSGRSSL TKDDLSRYK PTSREDLSS SSQKYINSR 
      FLPKNSVEK SYTAYTRPS AIRSHETIR KNRELLNVL 
      HAQQEQSRP TSRCDAKTP PKEPETETV STITRATSP 
      SPTTTNIAR SRRTEIAKT VEKQTTRAK NRPMSDKEV 
      QSDDTKNPR VSGAGRMVA TPWSSFLDM KFSSPNDQT 
      RKSVSRTNS SKSLEKKSP PKKTSPTKQ LLPPQIPKS 
      DSKSSSLHS ISTANKDFR KSVLNMNPE GGKKKVGRR 
      SNSASSAES EPEVQASEA TDVSENLTS CPSYHKSSS 
      SSSKLPQRV DKGGQRSRR SPSSDGSTS SSSASEEEK 
      KDLKVNSSR TSMAVSSAD ESPKPPPSP RLKSEAEAK 
      SFLMRALAP VTSLFKTKY QGSSDKINW MDESPPTTP 
      KSDQKPAKN FKKIESGER AWWLDDTTA SCSLPKDSE 
      KSQPKTKPF LRNIDSGEI PWWLDKNAE VPEGVQTYP 
      EESPQKIFR LGSTDTEPS SNIMDADYQ ERHKIRHID 
      SGERSWWLS SAENIPEMV QAQEKSEKP KYAIRHQES 
      GEKAWWLQS NSHSEEFDQ TQAPLGDRA SPEGLEMPE 
      GRLSPYDNV PVRSNKGKK TANLFISKH RNIDEILGG 
      CGPMWIFDY QGQEKEEKC MEVDAKEVR IHDSTPQRG 
      VIQPARIDN IGGFPKTFS PYGKYLNDI DPFSYTTNF 
      PDPARPPLH TFSVTLPLI NQLAAVHRL LKSPHRLDD 
      AALQLYKDG DYGSYLDLE ASISEQQEE FEGFQSNES 
      SLNPRLTKP LNMGYLLHE LIAYNNRVD AAPVPVLCL 
      ISDVRIFCE KSEEGLLTQ WLNSLETRS SLFWLHYVD 
      HDDDDKRAN SLESTEAST SEDDHVDDF LDVDDVPTC 
      RSIAHRESD LLLALTENC TLGPVCYFC RLIPIFCCS 
      KKNSIVLRT QLSVRVHTI IEKLLTSEG RELRRALFS 
      LKQIFQEDK DLVHEFVQN DGLSCLIKV GSEADQNYQ 
      NYILRALGQ VMLYVDGMN GVMEHNQTI QWLYSLISS 
      KFRLVVKTA LKLLLVFVE YTESNCLLL IKAIQAVDF 
      AQGVLPWHN IMKLLKDFD SADTELLIY ATTLINKVL 
      NGVPDQDTY YDQTDSLEE QEIERIIQR YMSKPGTDL 
      DLLQQFQIY EAVLQYEDG EEGGTPIRH LDESIRKTL 
      RTRKSVSEA NERRKSRRH STGTSPHPH PLLARNNNR 
      SLQINVEDD ESSSSQSSE INNSQLNGC YKDSNKATD 
      AGVTPALRR RRERAERQR SFMREQQET VELLRAASQ 
      NEEDENENN LGNYTNALQ QRIANRKDI TPLMNAVNK 
      LDSEENLTK QPWILYDDS QQPIDENTE NSNENERNV 
      LLQLKRENT VKDLTQKLA NQNILHSPS DENKPNRIG 
      DMTGLISKA KEGLAKSKS KADVLKSPT TESPPKIEV 
      KKSENELRW EELVANLDR PLNLCDMDF TDLGSDDEA 
      DVLAPAAVF NGIPPPPPP LKENGAPPP PPLSKGPPP 
      VPNPPVFGV TLPRMSKQA ETKIPIKKN KKTVKLFWK 
      EVREDPVCS SKLKTGFIW DELKPVCVD TQKLEHLFE 
      SRAKDLITK KQQEMNKNK EIIVLDPKR SNAINIGMT 
      KLPPPRSIK TAILKMDAT IMNREGIEK LLTMLPTEE 
      ERSKIQEAQ AANPDLPLG SAEQFLLTL ASISELPAR 
      LKLWAFKLD FENSEREIA EPLMDLKQG IEVLRVNKT 
      FRGILSTLL SIGNFLNGN EVKGFQIDY LAKVPEVKD 
      TVHKHSLLH HLCHIVMEK FPDATDLYS EIGAITRAS 
      KIDFDELAL NIQRLEADC KASWDHLKL IAKHDGSTM 
      MKVKMSDFL ADCAERIIL VGIIHRRVM NRFHKFLLW 
      LGIPLHQLS DTKPNEFCR IVSEFALEY RTTRERVLQ 
      QLEKKANHR ERNKTRGKM ITDAHAITV ASPLVTF
    CDS sequence
    • >TC032667 RA
      atgagcgcc tacagaaac tacgtgcct tggaacaaa 
      tcgccgaat tcctccaca agtttttcg tccaaaaaa 
      gaagggaca aaatacgat tcgagtttt ggcaagaag 
      aaggatgaa gaaaagagt gccacggcc aggattgcc 
      tcgaaatat ggcgcctat ggctcaggc taccagcga 
      gacagcagt gcttcagga agcagcagc tactcgagc 
      cggcttaaa ccaactctg gaaaaaaag gagacgaac 
      caccctccc gtctcttat cgggcttta gcccgaaca 
      accccccgg tcacgtgac cctagccct gacaaaagc 
      tcctcttct tcgagctac aactacaat cgcctttac 
      cccaccagg ccctacacc cgctctgtg agtagggaa 
      ctggacact tccaccacc ataccgaaa tattccgga 
      cgctcctca ctcagtaaa gatgacgtg tccagtcgg 
      cgttcgtcc gtaagccgg gaagactta tccaaatac 
      actggacgt tcttccaca acgaaggaa gacctagtc 
      ccgaaatat tcaggacgg tcttcactc accaaagac 
      gatttgtcg cgctataag ccgacaagt cgcgaagat 
      ttatcttca agttcccag aaatatatc aacagccgg 
      tttcttccg aaaaattct gttgagaaa agctatacg 
      gcctacacc aggccttct gcgatcaga tcgcacgaa 
      accattcgc aaaaatcgc gaactactc aatgtcttg 
      cacgcgcaa caagagcaa agtcggccc acaagtcgc 
      tgcgacgcc aaaacccct cccaaagaa ccggaaacg 
      gaaactgtc tcaaccata accagagcc acttcccct 
      tcccccacc accacaaac atcgctcgc tcccggcga 
      accgaaatc gccaaaacc gtcgaaaaa caaacaaca 
      cgtgccaaa aaccggcca atgagcgac aaggaagtc 
      caatcggac gacactaaa aatcccagg gtttcagga 
      gcgggccgt atggtggcc actccttgg agttcgttc 
      ctcgatatg aaattttcg agtccaaac gaccaaacc 
      cgcaaaagt gtctcacgg acgaactcc agcaaaagt 
      ctggagaag aaatcaccc cccaagaag acttccccc 
      accaagcaa ctcctccca ccccaaatc cccaaaagc 
      gactcgaaa tcatcgtcg ttacattcc attagcacc 
      gcgaataag gatttcagg aaatcggtc ctgaatatg 
      aaccctgaa ggggggaag aagaaggtt gggaggagg 
      agtaattcg gcgagttcg gctgagtcc gagcctgaa 
      gtccaggcg tcggaagca accgacgtt tcggagaat 
      ttgacgagt tgtccgtct tatcacaag agtagttct 
      tcgagttct aagttgccg cagagggtt gataagggg 
      gggcagagg agtcgaagg tcgccgagt tccgatggg 
      agcacctcg tcgtcgtct gcgagcgag gaggagaag 
      aaggatttg aaggttaac tcctcgagg acttccatg 
      gcggtgtcg tcagctgat gagtcgccg aaaccgcca 
      ccaagtcca agactcaag tcggaagcc gaagccaag 
      tcgtttcta atgagggct ttggcgccg gttacgagt 
      ttgttcaag accaagtat caaggatcc agtgacaaa 
      atcaactgg atggacgaa agccccccc accactcca 
      aaaagcgac caaaaaccg gcgaaaaac ttcaaaaaa 
      atcgaatcc ggtgaacgc gcctggtgg ttggacgac 
      accacagcc tcctgttcc ctcccaaag gactctgaa 
      aagagccaa ccgaaaacc aaacccttc ttacgcaat 
      atcgattcc ggggaaatc ccctggtgg ctcgacaaa 
      aacgccgaa gtccctgaa ggcgtccaa acgtacccc 
      gaggaatcc cctcagaaa attttccgt cttgggagt 
      actgacact gagcccagt agcaatatc atggacgct 
      gactaccaa gagcgccac aaaatcaga cacatcgac 
      tcaggagag cgctcttgg tggctcagc agcgctgag 
      aatatcccc gaaatggtg caagctcag gaaaagagc 
      gagaaaccg aaatatgca atcaggcat caggaatca 
      ggggagaaa gcctggtgg ttgcagtcg aactcacac 
      tcagaggag ttcgaccag actcaggcc cctttgggc 
      gatagggcg agtcctgag ggcttggaa atgccagaa 
      gggaggtta agtccctat gataatgtc cctgtgagg 
      agtaataag ggaaagaaa acagcgaat ttgtttatt 
      tccaaacat agaaatatt gacgagatt ttgggaggg 
      tgtgggccg atgtggatt tttgattac caaggtcag 
      gaaaaagag gagaagtgc atggaagtg gacgccaag 
      gaggtcagg attcacgac agtacgccc cagagggga 
      gtcattcag cccgctcgg attgacaac atcggtggt 
      ttcccgaag actttttcg ccgtacgga aagtatttg 
      aacgacatc gaccctttt tcgtacact acgaatttt 
      ccggatcct gcgagacct cctctgcac acttttagt 
      gtcactttg ccgctcatc aaccagctg gcagctgtt 
      cacaggctg ctcaagtcc ccacatagg ttggatgat 
      gcggcactt cagttgtac aaagatggg gattatggg 
      tcttacctt gacctcgaa gcttctatc agtgaacag 
      caagaagag tttgagggt tttcaatca aatgagagt 
      tctcttaac cccaggtta acaaaaccc ctcaatatg 
      ggttatctc ctccacgaa ctcatcgca tacaacaat 
      cgagtagac gctgcacct gtgccagtg ctgtgcttg 
      ataagtgat gtgaggata ttttgcgaa aaatccgag 
      gagggcctc ctcacccaa tggctcaac tctctggag 
      acgcgttcg tcgctcttc tggctccac tatgtggac 
      cacgacgac gacgacaaa cgcgccaat tcgctggaa 
      tcgacggag gcgtcgacc agcgaagac gaccacgtg 
      gacgacttc ctggacgtg gacgacgtg cccacatgt 
      cgcagtatc gcgcaccgg gagtccgac cttttgctg 
      gctctcacc gagaattgc actctgggc ccggtttgc 
      tatttttgc agactaatt cccattttc tgttgcagt 
      aagaagaat tcgatcgtt ttaaggaca cagctgtcg 
      gttagagtg cacacgatt atagaaaaa ttactaact 
      tccgaaggc cgcgaactg cgtcgcgca cttttctcc 
      ctcaaacaa atcttccaa gaggataaa gacctggtc 
      catgagttt gtccaaaac gatgggttg tcgtgtctc 
      atcaaagtc ggctctgaa gccgaccaa aactaccaa 
      aattacatt ttgcgggcc ttgggccaa gtgatgttg 
      tatgtggat gggatgaac ggggtgatg gaacacaac 
      caaaccatc caatggttg tacagtctc atttcgtca 
      aagttccga cttgtggtc aaaaccgcc ttgaagttg 
      cttttggtt tttgtggaa tatacggaa tcgaattgt 
      ttgctgttg atcaaagcg attcaagcg gttgatttt 
      gcacaaggt gttctgcct tggcacaac ataatgaaa 
      ctactcaaa gattttgat tccgctgac accgaactc 
      ctgatctat gctacaact ttgatcaat aaagtacta 
      aatggagta ccagatcaa gacacttat tacgaccaa 
      accgattca cttgaggag caagaaatc gagcgaata 
      atccaaaga tacatgtcc aagccggga actgatctg 
      gacttactc caacaattc caaatttac gaagccgtt 
      cttcaatac gaagacggc gaagaaggc ggaacccca 
      atccgccac ttggacgaa agtatccga aagacgctc 
      cgaacgcga aaatcagtc tcggaagcg aacgaacgc 
      agaaaaagt cggagacac tccaccggg acttccccg 
      caccctcac ccccttcta gcgcgcaac aacaacagg 
      tccctccag atcaacgtc gaagacgac gaatcgtca 
      agctcccag tcgtcggaa atcaacaat tcgcagctg 
      aatggatgc tataaggac agtaacaaa gcgactgac 
      gctggcgtc acccccgct ttgaggagg aggcgggaa 
      cgggcggag agacagagg agtttcatg cgggagcag 
      caggagacg gtggagttg ttgagggcg gcgtcccag 
      aatgaggag gacgagaat gagaataat ttgggaaac 
      tacacaaac gccctccag cagcgaatc gccaaccgc 
      aaagacatc acccctctc atgaacgca gtcaacaag 
      cttgactct gaagagaac ctcactaaa caaccctgg 
      atcctttac gacgactcc caacagcca atcgacgaa 
      aacaccgaa aattcgaac gaaaacgag cgaaatgtc 
      ctcctccaa ctcaaacgc gaaaacaca gtcaaagac 
      ctgacccaa aaattggcc aatcaaaat atcctccat 
      agtccttca gacgaaaac aaaccgaac cgaatcggc 
      gacatgacc ggtttaatt tccaaggcc aaagaaggc 
      ttagccaag tccaagtcc aaagcggac gtgctcaag 
      agcccaaca actgaatcc ccgccaaaa atcgaagta 
      aagaagtcc gaaaacgag ctcagatgg gaggagtta 
      gtcgcgaat ttagatcgt ccgttgaac ttgtgtgat 
      atggacttc actgatttg gggagcgac gacgaggcg 
      gatgttctg gccccagct gctgtgttc aatggaatc 
      cctccacct cctccccct ttgaaggag aacggggcg 
      cccccacct cccccctta tcaaagggg ccccctccc 
      gtcccaaac cccccagtt tttggggta accctccca 
      cgaatgtcc aaacaagcc gaaaccaaa atcccaatc 
      aagaaaaac aagaagaca gtaaaactc ttctggaag 
      gaagtccga gaggatcca gtttgctcc tccaaactc 
      aagactggt tttatttgg gacgaactg aaaccagtt 
      tgtgtcgac acccaaaaa ctggaacac ctgttcgag 
      tcaagggcc aaggacctc attacgaag aaacagcaa 
      gagatgaat aaaaacaag gaaataatc gtcctggac 
      ccgaaacgc tccaacgcc atcaacatc ggcatgact 
      aagttaccc cccccacgt tccatcaaa accgccatt 
      ttaaaaatg gacgccacc ataatgaac cgggaaggt 
      atcgaaaaa ttgctcaca atgctgcca actgaagag 
      gaaaggtct aaaatacaa gaagcccaa gctgccaat 
      cctgatctt cctctaggg agtgcggaa cagttcctt 
      ttaactcta gcatcaatc tctgaatta ccggcaaga 
      cttaaattg tgggctttt aagctcgat tttgaaaat 
      tccgaacgg gaaattgcc gaacccttg atggatttg 
      aagcaaggg attgaggtt ttgcgagtg aataagacg 
      tttaggggg attttgagc acccttttg tcgataggg 
      aattttttg aatgggaat gaggtgaaa gggttccaa 
      attgattat cttgccaaa gtacctgaa gtcaaagat 
      acggtccat aagcactct ttgttgcac catctttgc 
      catattgtt atggagaag ttcccagac gcgactgat 
      ttgtattca gagattggt gcaattact cgagcttcc 
      aaaatcgac ttcgacgaa cttgccttg aacatccag 
      aggcttgag gccgattgc aaggcctcc tgggaccac 
      ttgaaactc atagccaaa cacgatggt tccactatg 
      atgaaagtc aaaatgtcc gactttcta gccgattgt 
      gccgaaaga attattctg gttggaatc atccacagg 
      agggttatg aacagattt cataaattt ttattatgg 
      ttagggatc cctctacat caactgtcg gataccaag 
      ccaaatgag ttttgtaga atcgtttcc gagtttgcc 
      ttagaatat cgaacgaca agggaacga gttttacaa 
      cagttggaa aagaaagcc aatcatcgc gaacgcaat 
      aaaacgagg gggaaaatg ataacggat gcccacgcc 
      atcacagta gcttctccc ctagttact ttctga
    mRNA sequence
    • >TC032667 RA
      gtggttaga atagagcga gaacgtttt aataaattc 
      tatttgggc cgcgccagt tttttcaat tagtttaac 
      catttcggt ttagtgtgt gttgttgga tgatctttg 
      gtttatttg tgggttcaa acggtttcg acctcaaca 
      caatgtaaa cttggtcga ttttttgac aaattgtga 
      ctaattaat taatatgaa gtgaggtgt gagctattt 
      tcaaattat gagcgccta cagaaacta cgtgccttg 
      gaacaaatc gccgaattc ctccacaag tttttcgtc 
      caaaaaaga agggacaaa atacgattc gagttttgg 
      caagaagaa ggatgaaga aaagagtgc cacggccag 
      gattgcctc gaaatatgg cgcctatgg ctcaggcta 
      ccagcgaga cagcagtgc ttcaggaag cagcagcta 
      ctcgagccg gcttaaacc aactctgga aaaaaagga 
      gacgaacca ccctcccgt ctcttatcg ggctttagc 
      ccgaacaac cccccggtc acgtgaccc tagccctga 
      caaaagctc ctcttcttc gagctacaa ctacaatcg 
      cctttaccc caccaggcc ctacacccg ctctgtgag 
      tagggaact ggacacttc caccaccat accgaaata 
      ttccggacg ctcctcact cagtaaaga tgacgtgtc 
      cagtcggcg ttcgtccgt aagccggga agacttatc 
      caaatacac tggacgttc ttccacaac gaaggaaga 
      cctagtccc gaaatattc aggacggtc ttcactcac 
      caaagacga tttgtcgcg ctataagcc gacaagtcg 
      cgaagattt atcttcaag ttcccagaa atatatcaa 
      cagccggtt tcttccgaa aaattctgt tgagaaaag 
      ctatacggc ctacaccag gccttctgc gatcagatc 
      gcacgaaac cattcgcaa aaatcgcga actactcaa 
      tgtcttgca cgcgcaaca agagcaaag tcggcccac 
      aagtcgctg cgacgccaa aacccctcc caaagaacc 
      ggaaacgga aactgtctc aaccataac cagagccac 
      ttccccttc ccccaccac cacaaacat cgctcgctc 
      ccggcgaac cgaaatcgc caaaaccgt cgaaaaaca 
      aacaacacg tgccaaaaa ccggccaat gagcgacaa 
      ggaagtcca atcggacga cactaaaaa tcccagggt 
      ttcaggagc gggccgtat ggtggccac tccttggag 
      ttcgttcct cgatatgaa attttcgag tccaaacga 
      ccaaacccg caaaagtgt ctcacggac gaactccag 
      caaaagtct ggagaagaa atcaccccc caagaagac 
      ttcccccac caagcaact cctcccacc ccaaatccc 
      caaaagcga ctcgaaatc atcgtcgtt acattccat 
      tagcaccgc gaataagga tttcaggaa atcggtcct 
      gaatatgaa ccctgaagg ggggaagaa gaaggttgg 
      gaggaggag taattcggc gagttcggc tgagtccga 
      gcctgaagt ccaggcgtc ggaagcaac cgacgtttc 
      ggagaattt gacgagttg tccgtctta tcacaagag 
      tagttcttc gagttctaa gttgccgca gagggttga 
      taagggggg gcagaggag tcgaaggtc gccgagttc 
      cgatgggag cacctcgtc gtcgtctgc gagcgagga 
      ggagaagaa ggatttgaa ggttaactc ctcgaggac 
      ttccatggc ggtgtcgtc agctgatga gtcgccgaa 
      accgccacc aagtccaag actcaagtc ggaagccga 
      agccaagtc gtttctaat gagggcttt ggcgccggt 
      tacgagttt gttcaagac caagtatca aggatccag 
      tgacaaaat caactggat ggacgaaag cccccccac 
      cactccaaa aagcgacca aaaaccggc gaaaaactt 
      caaaaaaat cgaatccgg tgaacgcgc ctggtggtt 
      ggacgacac cacagcctc ctgttccct cccaaagga 
      ctctgaaaa gagccaacc gaaaaccaa acccttctt 
      acgcaatat cgattccgg ggaaatccc ctggtggct 
      cgacaaaaa cgccgaagt ccctgaagg cgtccaaac 
      gtaccccga ggaatcccc tcagaaaat tttccgtct 
      tgggagtac tgacactga gcccagtag caatatcat 
      ggacgctga ctaccaaga gcgccacaa aatcagaca 
      catcgactc aggagagcg ctcttggtg gctcagcag 
      cgctgagaa tatccccga aatggtgca agctcagga 
      aaagagcga gaaaccgaa atatgcaat caggcatca 
      ggaatcagg ggagaaagc ctggtggtt gcagtcgaa 
      ctcacactc agaggagtt cgaccagac tcaggcccc 
      tttgggcga tagggcgag tcctgaggg cttggaaat 
      gccagaagg gaggttaag tccctatga taatgtccc 
      tgtgaggag taataaggg aaagaaaac agcgaattt 
      gtttatttc caaacatag aaatattga cgagatttt 
      gggagggtg tgggccgat gtggatttt tgattacca 
      aggtcagga aaaagagga gaagtgcat ggaagtgga 
      cgccaagga ggtcaggat tcacgacag tacgcccca 
      gaggggagt cattcagcc cgctcggat tgacaacat 
      cggtggttt cccgaagac tttttcgcc gtacggaaa 
      gtatttgaa cgacatcga ccctttttc gtacactac 
      gaattttcc ggatcctgc gagacctcc tctgcacac 
      ttttagtgt cactttgcc gctcatcaa ccagctggc 
      agctgttca caggctgct caagtcccc acataggtt 
      ggatgatgc ggcacttca gttgtacaa agatgggga 
      ttatgggtc ttaccttga cctcgaagc ttctatcag 
      tgaacagca agaagagtt tgagggttt tcaatcaaa 
      tgagagttc tcttaaccc caggttaac aaaacccct 
      caatatggg ttatctcct ccacgaact catcgcata 
      caacaatcg agtagacgc tgcacctgt gccagtgct 
      gtgcttgat aagtgatgt gaggatatt ttgcgaaaa 
      atccgagga gggcctcct cacccaatg gctcaactc 
      tctggagac gcgttcgtc gctcttctg gctccacta 
      tgtggacca cgacgacga cgacaaacg cgccaattc 
      gctggaatc gacggaggc gtcgaccag cgaagacga 
      ccacgtgga cgacttcct ggacgtgga cgacgtgcc 
      cacatgtcg cagtatcgc gcaccggga gtccgacct 
      tttgctggc tctcaccga gaattgcac tctgggccc 
      ggtttgcta tttttgcag actaattcc cattttctg 
      ttgcagtaa gaagaattc gatcgtttt aaggacaca 
      gctgtcggt tagagtgca cacgattat agaaaaatt 
      actaacttc cgaaggccg cgaactgcg tcgcgcact 
      tttctccct caaacaaat cttccaaga ggataaaga 
      cctggtcca tgagtttgt ccaaaacga tgggttgtc 
      gtgtctcat caaagtcgg ctctgaagc cgaccaaaa 
      ctaccaaaa ttacatttt gcgggcctt gggccaagt 
      gatgttgta tgtggatgg gatgaacgg ggtgatgga 
      acacaacca aaccatcca atggttgta cagtctcat 
      ttcgtcaaa gttccgact tgtggtcaa aaccgcctt 
      gaagttgct tttggtttt tgtggaata tacggaatc 
      gaattgttt gctgttgat caaagcgat tcaagcggt 
      tgattttgc acaaggtgt tctgccttg gcacaacat 
      aatgaaact actcaaaga ttttgattc cgctgacac 
      cgaactcct gatctatgc tacaacttt gatcaataa 
      agtactaaa tggagtacc agatcaaga cacttatta 
      cgaccaaac cgattcact tgaggagca agaaatcga 
      gcgaataat ccaaagata catgtccaa gccgggaac 
      tgatctgga cttactcca acaattcca aatttacga 
      agccgttct tcaatacga agacggcga agaaggcgg 
      aaccccaat ccgccactt ggacgaaag tatccgaaa 
      gacgctccg aacgcgaaa atcagtctc ggaagcgaa 
      cgaacgcag aaaaagtcg gagacactc caccgggac 
      ttccccgca ccctcaccc ccttctagc gcgcaacaa 
      caacaggtc cctccagat caacgtcga agacgacga 
      atcgtcaag ctcccagtc gtcggaaat caacaattc 
      gcagctgaa tggatgcta taaggacag taacaaagc 
      gactgacgc tggcgtcac ccccgcttt gaggaggag 
      gcgggaacg ggcggagag acagaggag tttcatgcg 
      ggagcagca ggagacggt ggagttgtt gagggcggc 
      gtcccagaa tgaggagga cgagaatga gaataattt 
      gggaaacta cacaaacgc cctccagca gcgaatcgc 
      caaccgcaa agacatcac ccctctcat gaacgcagt 
      caacaagct tgactctga agagaacct cactaaaca 
      accctggat cctttacga cgactccca acagccaat 
      cgacgaaaa caccgaaaa ttcgaacga aaacgagcg 
      aaatgtcct cctccaact caaacgcga aaacacagt 
      caaagacct gacccaaaa attggccaa tcaaaatat 
      cctccatag tccttcaga cgaaaacaa accgaaccg 
      aatcggcga catgaccgg tttaatttc caaggccaa 
      agaaggctt agccaagtc caagtccaa agcggacgt 
      gctcaagag cccaacaac tgaatcccc gccaaaaat 
      cgaagtaaa gaagtccga aaacgagct cagatggga 
      ggagttagt cgcgaattt agatcgtcc gttgaactt 
      gtgtgatat ggacttcac tgatttggg gagcgacga 
      cgaggcgga tgttctggc cccagctgc tgtgttcaa 
      tggaatccc tccacctcc tcccccttt gaaggagaa 
      cggggcgcc cccacctcc ccccttatc aaaggggcc 
      ccctcccgt cccaaaccc cccagtttt tggggtaac 
      cctcccacg aatgtccaa acaagccga aaccaaaat 
      cccaatcaa gaaaaacaa gaagacagt aaaactctt 
      ctggaagga agtccgaga ggatccagt ttgctcctc 
      caaactcaa gactggttt tatttggga cgaactgaa 
      accagtttg tgtcgacac ccaaaaact ggaacacct 
      gttcgagtc aagggccaa ggacctcat tacgaagaa 
      acagcaaga gatgaataa aaacaagga aataatcgt 
      cctggaccc gaaacgctc caacgccat caacatcgg 
      catgactaa gttaccccc cccacgttc catcaaaac 
      cgccatttt aaaaatgga cgccaccat aatgaaccg 
      ggaaggtat cgaaaaatt gctcacaat gctgccaac 
      tgaagagga aaggtctaa aatacaaga agcccaagc 
      tgccaatcc tgatcttcc tctagggag tgcggaaca 
      gttcctttt aactctagc atcaatctc tgaattacc 
      ggcaagact taaattgtg ggcttttaa gctcgattt 
      tgaaaattc cgaacggga aattgccga acccttgat 
      ggatttgaa gcaagggat tgaggtttt gcgagtgaa 
      taagacgtt tagggggat tttgagcac ccttttgtc 
      gatagggaa ttttttgaa tgggaatga ggtgaaagg 
      gttccaaat tgattatct tgccaaagt acctgaagt 
      caaagatac ggtccataa gcactcttt gttgcacca 
      tctttgcca tattgttat ggagaagtt cccagacgc 
      gactgattt gtattcaga gattggtgc aattactcg 
      agcttccaa aatcgactt cgacgaact tgccttgaa 
      catccagag gcttgaggc cgattgcaa ggcctcctg 
      ggaccactt gaaactcat agccaaaca cgatggttc 
      cactatgat gaaagtcaa aatgtccga ctttctagc 
      cgattgtgc cgaaagaat tattctggt tggaatcat 
      ccacaggag ggttatgaa cagatttca taaattttt 
      attatggtt agggatccc tctacatca actgtcgga 
      taccaagcc aaatgagtt ttgtagaat cgtttccga 
      gtttgcctt agaatatcg aacgacaag ggaacgagt 
      tttacaaca gttggaaaa gaaagccaa tcatcgcga 
      acgcaataa aacgagggg gaaaatgat aacggatgc 
      ccacgccat cacagtagc ttctcccct agttacttt 
      ctgactacc aacctcata acctaaact aactaaata 
      ttctaacac tcggtcatt ctgaccttc gagcggacc 
      tccgctcga cataaaaca aggagcaat gaaatttaa 
      tacactaat gaaacaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY