Status
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Gene information
General information
TC002447 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 29473051..29485368
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002447
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02744

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002447 RA
      MLQSKCRHS SALPLETCS FCNSSSPES LLDICFNYI 
      NNNLDTICV SCKITGRLR LKEGVHLPV EICEKFLNV 
      RSNSLVRVN SNFIHIFRD RQSTRLKRV RLRDIDITD 
      RDLEVLLRH RLSELDVSY VPNLSSGCI KHLTEYGTL 
      LTSLTIGEE TEIFPTKIF GKLKFTEEH YDRGFIFLA 
      PNLKKLTLK RLNALQPDF FVLLLRHFS ALTHLDLSG 
      CSDLGGFEY TEHLVNLTS LVLYNVTGL ENMVPAICK 
      LKSLRHLDI SQSREENGR YENGGQILT TIVNNLPKL 
      TSLDISGTN LAGRGVADV ASGPVASDI PALSSRMNN 
      PFQYLGLYE TSHDACLRH DIPAKLIAG NANEEQILV 
      AALAFLDRT DMLQKVLNE LFHLLRFES CAYVGQALN 
      IVLDAMNRH LTERHVQIS GSATLFYIV KGTGKQLHD 
      AVRVKRRII TALLNGMSV HRTDETMMR NGCLTLCQF 
      KIPSDVLFD YERLVDILL HSVHGMAQE SFVQRIGIY 
      LLNSLACQV DRQQKAKLG ELGAIDKMI WLIADRLER 
      GHCDDVLEV AWSTMWNVT DETPSNCRK FLEIKGMEY 
      FLQCLEKFP NKDELLRNM MGLLGNVAE VRELRHFLV 
      TSKYLTVFS DLLDSRSDG IEVSYNAAG VISHIASDG 
      PDVWLVFQP TRAYVLNKM VRAIDRWDL NSQRNINYR 
      SFEPILYLV KVYHTPECQ KWAVWALAN MTKVYPEKY 
      CSLIEKEGG LELLQELIE HPSPPAPVK NLAHLVIEN 
      CRRYKEQEW TNLEAQVEV QLDG
    CDS sequence
    • >TC002447 RA
      atgcttcaa tccaaatgt cggcattcg agtgcccta 
      cctttagaa acgtgctcg ttttgcaac tcatcctcc 
      cccgaaagc ctgctcgat atttgcttc aactacatc 
      aacaacaac ctggacacg atatgtgtc tcctgcaag 
      atcacaggc cggttgcgc ctcaaagaa ggggtgcat 
      ttacccgtc gagatctgc gagaagttc ctcaacgtg 
      cggtcgaat agcctcgtg agagtcaac tcgaacttc 
      atccacata ttcagggac aggcaaagt acgaggctc 
      aagcgggtg cgtttacgg gacattgac atcacagac 
      agggacctg gaggtgctg ctaaggcac cgcctgtcc 
      gaactcgac gtttcgtac gtccccaac ctctcctca 
      ggctgcatc aagcacttg accgagtac ggcaccctg 
      ctcacttct ctaacaatc ggcgaagaa acggagatt 
      ttcccgacg aaaatcttc ggcaaattg aaattcacc 
      gaggaacat tacgaccgc gggtttatt ttcttggcc 
      cccaatctc aaaaaactg accctgaag cgcttaaac 
      gccctccag ccggacttt ttcgtcttg ttgttgcgg 
      catttctcc gctttgacc cacttggat ttgtcgggt 
      tgtagcgac ttggggggg tttgagtac acggaacat 
      ttagtgaat ttgacctcg ctggtgctg tacaatgtg 
      acggggttg gaaaatatg gtcccggcg atttgtaaa 
      ttgaagtcg ctgcgacac ctcgatatt tcgcagtcg 
      agggaagag aacgggagg tatgagaat ggggggcag 
      attctgacg acgattgtt aataatttg cccaaattg 
      acatcgctt gatatctcg gggacgaat ttggcgggc 
      cggggggtg gctgatgtg gcctcaggg cctgtggcg 
      tccgatatt ccagcgctg agttcgagg atgaataac 
      ccgtttcag tatttgggg ctgtatgag acctcgcat 
      gatgcctgt ctcaggcac gatattcca gcgaaattg 
      attgctgga aacgcgaac gaagagcaa attttagtc 
      gcagcatta gcttttctc gaccgcact gacatgctc 
      caaaaagtc ctcaacgag cttttccat ctgttacgt 
      ttcgaaagt tgtgcctac gttggtcaa gctttaaac 
      atagttttg gacgcaatg aatcggcat ttaaccgaa 
      agacacgta caaatttcg ggcagtgcc acacttttt 
      tacattgtc aaaggtaca ggaaaacaa cttcatgac 
      gctgttagg gtaaaaaga cgaataatt acggcttta 
      ttgaacgga atgagtgtg cataggacg gacgaaacg 
      atgatgagg aacgggtgc ttgacgttg tgtcagttt 
      aagattcct tcggatgtg ctttttgat tacgaacga 
      ttggtcgat attctgctc cattcggtt catggaatg 
      gcacaagag agtttcgtc caaaggata ggaatttat 
      cttttaaat tctctagct tgtcaagtc gataggcaa 
      caaaaagcg aaattagga gaacttgga gctattgat 
      aaaatgatt tggttgatt gctgataga ctggaaaga 
      ggacactgt gatgatgtt ttagaggtg gcttggtca 
      actatgtgg aacgtcacc gatgaaacg ccttctaat 
      tgtagaaaa tttttagaa atcaaagga atggagtat 
      ttcttgcag tgtctcgag aaattcccc aacaaagac 
      gaacttctt cgaaacatg atgggtctc ttaggcaac 
      gtggccgaa gttcgtgaa ttgcgccac tttcttgtc 
      acttccaaa tatctaaca gttttttcg gaccttttg 
      gattcgaga agtgacgga atcgaagtg agttacaac 
      gctgccggt gtcatatcg catattgca tcagatggg 
      cccgatgtt tggctcgtt tttcaacca acaagagct 
      tacgttttg aataaaatg gtgcgagct atagaccgg 
      tgggatttg aattcgcaa aggaatatc aattatcgg 
      tcgtttgag ccgattttg tatctggtc aaagtgtat 
      cacacgcca gagtgccag aaatgggcc gtgtgggcg 
      ctggccaac atgaccaaa gtttatcct gaaaagtac 
      tgctccttg atcgagaaa gagggcggt ttggagttg 
      ctccaggag ttgatcgaa catccatct cctccagct 
      cctgtgaag aacttagcc catttggtg attgaaaat 
      tgccggagg tacaaggag caggagtgg acgaacttg 
      gaggctcaa gtggaggtg cagctcgac ggttaa
    mRNA sequence
    • >TC002447 RA
      aaatatgtc aaaatgtca aaaattacc taataacaa 
      aaatagttg ggaaaacat ctggaaaag tgttgtgaa 
      aaattaacc gcctgcacg ttggccccg tctgcgaag 
      cgccccctt ccaatgtca tgtttctcc gcggaattg 
      actgaatta tcgcaaaat gcttcaatc caaatgtcg 
      gcattcgag tgccctacc tttagaaac gtgctcgtt 
      ttgcaactc atcctcccc cgaaagcct gctcgatat 
      ttgcttcaa ctacatcaa caacaacct ggacacgat 
      atgtgtctc ctgcaagat cacaggccg gttgcgcct 
      caaagaagg ggtgcattt acccgtcga gatctgcga 
      gaagttcct caacgtgcg gtcgaatag cctcgtgag 
      agtcaactc gaacttcat ccacatatt cagggacag 
      gcaaagtac gaggctcaa gcgggtgcg tttacggga 
      cattgacat cacagacag ggacctgga ggtgctgct 
      aaggcaccg cctgtccga actcgacgt ttcgtacgt 
      ccccaacct ctcctcagg ctgcatcaa gcacttgac 
      cgagtacgg caccctgct cacttctct aacaatcgg 
      cgaagaaac ggagatttt cccgacgaa aatcttcgg 
      caaattgaa attcaccga ggaacatta cgaccgcgg 
      gtttatttt cttggcccc caatctcaa aaaactgac 
      cctgaagcg cttaaacgc cctccagcc ggacttttt 
      cgtcttgtt gttgcggca tttctccgc tttgaccca 
      cttggattt gtcgggttg tagcgactt gggggggtt 
      tgagtacac ggaacattt agtgaattt gacctcgct 
      ggtgctgta caatgtgac ggggttgga aaatatggt 
      cccggcgat ttgtaaatt gaagtcgct gcgacacct 
      cgatatttc gcagtcgag ggaagagaa cgggaggta 
      tgagaatgg ggggcagat tctgacgac gattgttaa 
      taatttgcc caaattgac atcgcttga tatctcggg 
      gacgaattt ggcgggccg gggggtggc tgatgtggc 
      ctcagggcc tgtggcgtc cgatattcc agcgctgag 
      ttcgaggat gaataaccc gtttcagta tttggggct 
      gtatgagac ctcgcatga tgcctgtct caggcacga 
      tattccagc gaaattgat tgctggaaa cgcgaacga 
      agagcaaat tttagtcgc agcattagc ttttctcga 
      ccgcactga catgctcca aaaagtcct caacgagct 
      tttccatct gttacgttt cgaaagttg tgcctacgt 
      tggtcaagc tttaaacat agttttgga cgcaatgaa 
      tcggcattt aaccgaaag acacgtaca aatttcggg 
      cagtgccac actttttta cattgtcaa aggtacagg 
      aaaacaact tcatgacgc tgttagggt aaaaagacg 
      aataattac ggctttatt gaacggaat gagtgtgca 
      taggacgga cgaaacgat gatgaggaa cgggtgctt 
      gacgttgtg tcagtttaa gattccttc ggatgtgct 
      ttttgatta cgaacgatt ggtcgatat tctgctcca 
      ttcggttca tggaatggc acaagagag tttcgtcca 
      aaggatagg aatttatct tttaaattc tctagcttg 
      tcaagtcga taggcaaca aaaagcgaa attaggaga 
      acttggagc tattgataa aatgatttg gttgattgc 
      tgatagact ggaaagagg acactgtga tgatgtttt 
      agaggtggc ttggtcaac tatgtggaa cgtcaccga 
      tgaaacgcc ttctaattg tagaaaatt tttagaaat 
      caaaggaat ggagtattt cttgcagtg tctcgagaa 
      attccccaa caaagacga acttcttcg aaacatgat 
      gggtctctt aggcaacgt ggccgaagt tcgtgaatt 
      gcgccactt tcttgtcac ttccaaata tctaacagt 
      tttttcgga ccttttgga ttcgagaag tgacggaat 
      cgaagtgag ttacaacgc tgccggtgt catatcgca 
      tattgcatc agatgggcc cgatgtttg gctcgtttt 
      tcaaccaac aagagctta cgttttgaa taaaatggt 
      gcgagctat agaccggtg ggatttgaa ttcgcaaag 
      gaatatcaa ttatcggtc gtttgagcc gattttgta 
      tctggtcaa agtgtatca cacgccaga gtgccagaa 
      atgggccgt gtgggcgct ggccaacat gaccaaagt 
      ttatcctga aaagtactg ctccttgat cgagaaaga 
      gggcggttt ggagttgct ccaggagtt gatcgaaca 
      tccatctcc tccagctcc tgtgaagaa cttagccca 
      tttggtgat tgaaaattg ccggaggta caaggagca 
      ggagtggac gaacttgga ggctcaagt ggaggtgca 
      gctcgacgg ttaatttgt tatattatt tatgtttac 
      tttattaat acttcggaa cgatggaaa ttcctttgt 
      gattgattg cgttgatta tataatttt attttttta 
      ttttcttca agggtaatc gtaaccaca tttattgcg 
      catgtgtgg actgttagt taaactaat tgctactgt 
      acataattt atttccaat atgatgtat taaatgggt 
      tagccttac tctgtggaa ttagtttct ctattttat 
      catgacatt attattatt atgttattt atgccattt 
      aattatatt ttatttatc taagtctag gttttttac 
      ctgtcctat tatcacccg tttgtaagc ggaagtcga 
      tggtggcac ttttcggcg aatttgtta cagcttgaa 
      taaataatt taataaatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY