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Gene information
General information
TC031068 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 27069578..27106570
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031068
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02738

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031068 RA
      MRRKCEEVS GSSADYGPM STEDVQVDV KYSDDLGNK 
      TPKQLLLNS TTTVSSIEM FVRDEFPND HESLDLVLQ 
      YEDGKEICL SDHKTSCMA DLGITLTPG HNYTILVSP 
      SEKRNMSLY VNDVTEDDL LLGASASPT TGEVLSSSA 
      SDSAAPIDD YPEHGHCSR CRIGPTGEE SSNNVEAMS 
      VVETTNYVG LVNQAMTCY LNSLVQALF MTPEFRNAI 
      YNWEFDGQN EARSIPYQL QKLFLNLQT STRSAVETT 
      DLTTSFGWQ GSEAWHQHD IQELCRVMF DALERKFKD 
      TKQANLIND LYEGTMLDY VKCLECGTE KSREDTFLD 
      IPLPVRPFG STVAYNSVE EALRAFVQP ETLEGNNQY 
      HCEKCNKKC DAHKGLKFI KFPYLLTLH LKRFDFDYN 
      TMHRIKLND KVVFPEKLN LNSFVPNNR SENDGLEER 
      DTVVRCDEC STTDSGSAL DDESCQGTD LSSTVNDHD 
      DNCEDSDEG IDVSSGNAH HENDAKGPY IYELFSIMI 
      HSGSASGGH YYAYIKDFD KNQWLCFND QSVNRITEE 
      DIKKTYGGG PQKGYYSGV YTSSTNAYM LMYRQIDKD 
      RNCGAMKVE EFPPHMQKL LQNMRNKEK TDRLNKEKQ 
      NNSFRLTVN CYHPYTEEM MDVRISLYL DSTLEDAVQ 
      MAHRRFKLE GIVNVDDCR MVIFNKKQS CIDCSFESD 
      DLKFCDIDD NYKVCYQEW LLDIREPGT EFTTYKKGG 
      VNKRTYYVN IETEDVREP FMVRIDVGD TVGDVKNKI 
      AEQLETNVN NIKVAIAKF CNEGKYLDD DNATLAIDN 
      TSPDFKLYV TNAIDEDPD KSFENSKFK KIIDRFENI 
      VYLSVLLPD TDPVTLDGL FIPNLDPDK LGAVTTDRT 
      NSPSLRISP QPGVLSCGD NFGDQSNSE DSSLSDSDR 
      TLVGDAPGE CQGIFSSSS TSPADQHMT SPMDPAEDM 
      YNYDVLGNP SEEMNWDSH ATGVIRNAT FFKLLNTRD 
      CRRGVSDAN YAKICKVFV DNRMTLETF KKYLESVVR 
      VPKEYFKIY RQYPSPDEE WSNLSDTLR LARDGQTLT 
      VKLGRALAI NEFSCKVYL LRPESTETN HFLFEHILT 
      RGQSVGQVK KEILFKAKK QHMLDIQFN RCRLREKCL 
      KKPRKVYLD DQTFSDDIL VLSSYELYL QELPYPEKV 
      TSTNQLVLF ARHWCPATM TLEPFQEVI IDTPTMSEL 
      KTKVAELSN IPVENVQVA FVKSTFPCD MHLLDIHTE 
      LEWDPNVTH LYQWPLQVD DGTAFFYRD GREELKELT 
      YEEKKEINI RENTRLGRV PLKSCATLE KSLRIYLDN 
      WPSETEDNC ID
    CDS sequence
    • >TC031068 RA
      atgaggagg aaatgtgag gaggtgtcg ggctcttcg 
      gccgattac gggccgatg agtactgaa gatgtccaa 
      gtcgatgtg aaatacagc gatgatctt gggaacaaa 
      actccaaaa caactgctc ctgaactcc actacgaca 
      gtgtcgagt attgagatg tttgtccga gatgagttt 
      ccaaacgat cacgaaagt cttgatctc gtactccag 
      tatgaggat ggaaaagaa atttgcctc agcgaccac 
      aaaaccagc tgtatggca gacctcggc ataacccta 
      acacctgga cataattac acaattctc gtttcacca 
      tcagaaaaa cgcaacatg tctttgtac gttaacgac 
      gtcaccgaa gatgattta ctattagga gcttcggct 
      agtccaaca accggtgaa gttttatca tctagtgca 
      agcgatagt gctgcccct atcgacgat tatcccgaa 
      catggtcat tgttcgaga tgtcgtatt ggaccgact 
      ggtgaagaa tcgtccaat aatgttgaa gcaatgagt 
      gttgttgaa actacgaat tatgtcggt ctagtcaac 
      caagcaatg acgtgttat ttgaacagt ttggttcaa 
      gcgttgttt atgacacca gaattcaga aacgccatc 
      tataattgg gagtttgat ggacagaat gaggccagg 
      tctataccc tatcagctg cagaagttg ttcctcaat 
      ttacagacg tcaacgagg tcagccgtt gagaccacc 
      gacctaacc accagcttc ggttggcaa ggaagtgaa 
      gcatggcac cagcacgac atccaagag ctgtgccga 
      gtcatgttc gatgcgtta gagcgcaaa ttcaaagac 
      accaaacag gcaaatcta attaacgat ttgtacgaa 
      ggcaccatg ttagactat gtcaagtgt ttagagtgc 
      ggtacggaa aaatcccgc gaggatacg ttcctagat 
      attccgttg ccggtgaga ccgtttggt agtacagta 
      gcgtacaat agtgtcgaa gaagcgctg agagcgttt 
      gtccaaccg gaaacattg gaaggaaac aatcaatat 
      cattgcgag aaatgtaat aagaagtgt gacgcacac 
      aaaggtttg aaatttata aaattccct tatttactc 
      acattgcat ttaaagagg ttcgatttt gactacaac 
      actatgcac agaatcaag ttgaacgat aaggttgtg 
      tttcctgaa aaacttaat ttaaattct ttcgtgcct 
      aataatagg agtgaaaat gatgggttg gaggagaga 
      gatacggtt gtaaggtgt gatgagtgt agtacgacg 
      gattctgga tcggctttg gatgatgag agctgtcag 
      ggtactgat ttgtcttcc actgttaat gatcatgac 
      gataactgc gaggatagt gatgaagga atcgacgta 
      agttccggc aacgcacac cacgaaaac gacgccaaa 
      ggcccctac atttacgaa ctgttcagc ataatgatc 
      cactcaggc agcgcctct ggtggtcac tactatgct 
      tacatcaaa gatttcgac aaaaaccaa tggctgtgt 
      tttaacgat cagagcgta aaccgcatc accgaagaa 
      gacattaaa aaaacctac ggtggcggc ccccaaaaa 
      ggctactat tcaggcgtc tacacctct agtacaaac 
      gcctacatg ttaatgtat cgtcaaatc gacaaagac 
      cgaaactgt ggcgcaatg aaagtcgag gaatttcca 
      ccgcacatg caaaaattg ctccagaat atgcgaaat 
      aaggagaag accgatcga ttgaataaa gaaaagcaa 
      aataattcg ttccggctg acggttaat tgttaccat 
      ccatatacg gaggagatg atggatgtg aggattagt 
      ttgtacttg gattcgacg ctggaagat gccgtccag 
      atggcgcat cgtcggttc aagttggaa gggattgtg 
      aatgttgat gattgcagg atggttatt tttaataag 
      aaacaatca tgtatcgac tgtagtttc gagtccgat 
      gaccttaaa ttctgtgat attgacgat aattataaa 
      gtgtgttat caggaatgg ctgttagac atcagggag 
      ccaggaacg gagtttaca acctacaag aaaggagga 
      gtcaacaag agaacatac tacgtaaat atcgaaaca 
      gaggacgtc agggaaccg tttatggtg cggatcgac 
      gttggcgat actgttggt gatgtaaaa aataaaatt 
      gccgaacag ttggaaacg aacgttaat aacatcaag 
      gttgcaatt gcgaaattt tgcaatgag ggcaaatac 
      ttagatgac gacaacgcg acgcttgct atcgataat 
      acctcccct gatttcaaa ctgtacgtt acaaatgcg 
      atagatgag gatccagat aagagtttt gaaaattct 
      aagtttaag aaaattatt gataggttt gagaatatc 
      gtttatttg agtgttttg ttgccggat actgatcca 
      gtgactttg gatggtttg tttatacca aatttggat 
      cctgataaa ttgggtgct gtaacgact gatcgaacc 
      aattctcct agtcttagg atttcgcca caaccgggc 
      gttttgagt tgtggcgat aatttcggt gatcagagc 
      aacagtgag gatagtagt ttgagtgat agcgatagg 
      acgcttgta ggagatgca ccaggtgaa tgccagggc 
      attttctcg agcagttcc acatcgccc gccgaccag 
      cacatgacc tcccccatg gaccccgcc gaagacatg 
      tacaactac gacgttctc ggcaatccg tccgaagaa 
      atgaactgg gattcccac gcaactggc gtcatccgc 
      aacgccacc tttttcaaa ctcttgaac acgcgcgat 
      tgccggcgc ggcgtttcg gacgcgaat tacgcgaaa 
      atatgcaaa gtgttcgtc gataatcgc atgacttta 
      gaaacgttt aaaaaatat ctagaatcg gtagttagg 
      gttcctaag gaatatttt aaaatttac cgacagtat 
      ccgagtccg gacgaggaa tggtcgaac ttatccgac 
      actttacga ctggctcgc gacggtcaa acgctcact 
      gttaagttg ggccgagcg ttggcgatc aacgaattc 
      agttgtaag gtttatttg ttgcgaccg gaaagcacc 
      gaaaccaat cacttccta ttcgaacat atcttaact 
      aggggacaa tccgtaggc caagtcaag aaggagatt 
      ttgttcaag gcgaaaaag cagcacatg ttggatata 
      cagtttaat agatgtcgt ctgagggag aagtgtttg 
      aaaaagccg aggaaagtt tatctcgat gatcaaacc 
      ttctcggat gatattcta gtgttgagc agttacgag 
      ctgtacctc caggagtta ccatatccg gagaaagtt 
      acttcaacc aatcagctt gttttgttt gcgcgacat 
      tggtgtccg gcaacgatg acacttgag ccgtttcag 
      gaagttatt attgatact ccgaccatg tccgaatta 
      aaaacgaaa gtggccgaa ctttcgaat attcccgtc 
      gaaaatgtc caagtagca tttgtcaaa agtacattt 
      ccttgcgat atgcatttg ttagatata cacactgag 
      ttggagtgg gatccgaac gtgacgcat ttgtatcag 
      tggcctctg caggtggat gatggaact gcctttttc 
      tacagagat gggcgcgag gagctgaaa gagttaacg 
      tatgaggag aagaaagag attaacata agggagaac 
      acgcgcttg ggtcgcgtc cccttgaag agttgtgcc 
      acgttggag aagtcgttg cgtatttat ttagataat 
      tggccttct gaaacagag gacaattgt atagactga
    mRNA sequence
    • >TC031068 RA
      gacttgtgc acaaattgg aaaaaatcc acaaaaagg 
      tgtgatttt ttcattagt agataataa ataattgat 
      aattgggac tcaaaatcg cacgtagtg tttttctca 
      ttgtaattt aacgaattt ggggtttaa agtctagtg 
      tgtttacgt ttgggattg gcattcctg ctcaaaacg 
      gggcaaatg gtggtgaaa agtgagcaa gatggcgcg 
      tgatgaatt ttagcacat gtcaaatat ttttgcgaa 
      attggaggc gccctgaaa aatgaggag gaaatgtga 
      ggaggtgtc gggctcttc ggccgatta cgggccgat 
      gagtactga agatgtcca agtcgatgt gaaatacag 
      cgatgatct tgggaacaa aactccaaa acaactgct 
      cctgaactc cactacgac agtgtcgag tattgagat 
      gtttgtccg agatgagtt tccaaacga tcacgaaag 
      tcttgatct cgtactcca gtatgagga tggaaaaga 
      aatttgcct cagcgacca caaaaccag ctgtatggc 
      agacctcgg cataaccct aacacctgg acataatta 
      cacaattct cgtttcacc atcagaaaa acgcaacat 
      gtctttgta cgttaacga cgtcaccga agatgattt 
      actattagg agcttcggc tagtccaac aaccggtga 
      agttttatc atctagtgc aagcgatag tgctgcccc 
      tatcgacga ttatcccga acatggtca ttgttcgag 
      atgtcgtat tggaccgac tggtgaaga atcgtccaa 
      taatgttga agcaatgag tgttgttga aactacgaa 
      ttatgtcgg tctagtcaa ccaagcaat gacgtgtta 
      tttgaacag tttggttca agcgttgtt tatgacacc 
      agaattcag aaacgccat ctataattg ggagtttga 
      tggacagaa tgaggccag gtctatacc ctatcagct 
      gcagaagtt gttcctcaa tttacagac gtcaacgag 
      gtcagccgt tgagaccac cgacctaac caccagctt 
      cggttggca aggaagtga agcatggca ccagcacga 
      catccaaga gctgtgccg agtcatgtt cgatgcgtt 
      agagcgcaa attcaaaga caccaaaca ggcaaatct 
      aattaacga tttgtacga aggcaccat gttagacta 
      tgtcaagtg tttagagtg cggtacgga aaaatcccg 
      cgaggatac gttcctaga tattccgtt gccggtgag 
      accgtttgg tagtacagt agcgtacaa tagtgtcga 
      agaagcgct gagagcgtt tgtccaacc ggaaacatt 
      ggaaggaaa caatcaata tcattgcga gaaatgtaa 
      taagaagtg tgacgcaca caaaggttt gaaatttat 
      aaaattccc ttatttact cacattgca tttaaagag 
      gttcgattt tgactacaa cactatgca cagaatcaa 
      gttgaacga taaggttgt gtttcctga aaaacttaa 
      tttaaattc tttcgtgcc taataatag gagtgaaaa 
      tgatgggtt ggaggagag agatacggt tgtaaggtg 
      tgatgagtg tagtacgac ggattctgg atcggcttt 
      ggatgatga gagctgtca gggtactga tttgtcttc 
      cactgttaa tgatcatga cgataactg cgaggatag 
      tgatgaagg aatcgacgt aagttccgg caacgcaca 
      ccacgaaaa cgacgccaa aggccccta catttacga 
      actgttcag cataatgat ccactcagg cagcgcctc 
      tggtggtca ctactatgc ttacatcaa agatttcga 
      caaaaacca atggctgtg ttttaacga tcagagcgt 
      aaaccgcat caccgaaga agacattaa aaaaaccta 
      cggtggcgg cccccaaaa aggctacta ttcaggcgt 
      ctacacctc tagtacaaa cgcctacat gttaatgta 
      tcgtcaaat cgacaaaga ccgaaactg tggcgcaat 
      gaaagtcga ggaatttcc accgcacat gcaaaaatt 
      gctccagaa tatgcgaaa taaggagaa gaccgatcg 
      attgaataa agaaaagca aaataattc gttccggct 
      gacggttaa ttgttacca tccatatac ggaggagat 
      gatggatgt gaggattag tttgtactt ggattcgac 
      gctggaaga tgccgtcca gatggcgca tcgtcggtt 
      caagttgga agggattgt gaatgttga tgattgcag 
      gatggttat ttttaataa gaaacaatc atgtatcga 
      ctgtagttt cgagtccga tgaccttaa attctgtga 
      tattgacga taattataa agtgtgtta tcaggaatg 
      gctgttaga catcaggga gccaggaac ggagtttac 
      aacctacaa gaaaggagg agtcaacaa gagaacata 
      ctacgtaaa tatcgaaac agaggacgt cagggaacc 
      gtttatggt gcggatcga cgttggcga tactgttgg 
      tgatgtaaa aaataaaat tgccgaaca gttggaaac 
      gaacgttaa taacatcaa ggttgcaat tgcgaaatt 
      ttgcaatga gggcaaata cttagatga cgacaacgc 
      gacgcttgc tatcgataa tacctcccc tgatttcaa 
      actgtacgt tacaaatgc gatagatga ggatccaga 
      taagagttt tgaaaattc taagtttaa gaaaattat 
      tgataggtt tgagaatat cgtttattt gagtgtttt 
      gttgccgga tactgatcc agtgacttt ggatggttt 
      gtttatacc aaatttgga tcctgataa attgggtgc 
      tgtaacgac tgatcgaac caattctcc tagtcttag 
      gatttcgcc acaaccggg cgttttgag ttgtggcga 
      taatttcgg tgatcagag caacagtga ggatagtag 
      tttgagtga tagcgatag gacgcttgt aggagatgc 
      accaggtga atgccaggg cattttctc gagcagttc 
      cacatcgcc cgccgacca gcacatgac ctcccccat 
      ggaccccgc cgaagacat gtacaacta cgacgttct 
      cggcaatcc gtccgaaga aatgaactg ggattccca 
      cgcaactgg cgtcatccg caacgccac ctttttcaa 
      actcttgaa cacgcgcga ttgccggcg cggcgtttc 
      ggacgcgaa ttacgcgaa aatatgcaa agtgttcgt 
      cgataatcg catgacttt agaaacgtt taaaaaata 
      tctagaatc ggtagttag ggttcctaa ggaatattt 
      taaaattta ccgacagta tccgagtcc ggacgagga 
      atggtcgaa cttatccga cactttacg actggctcg 
      cgacggtca aacgctcac tgttaagtt gggccgagc 
      gttggcgat caacgaatt cagttgtaa ggtttattt 
      gttgcgacc ggaaagcac cgaaaccaa tcacttcct 
      attcgaaca tatcttaac taggggaca atccgtagg 
      ccaagtcaa gaaggagat tttgttcaa ggcgaaaaa 
      gcagcacat gttggatat acagtttaa tagatgtcg 
      tctgaggga gaagtgttt gaaaaagcc gaggaaagt 
      ttatctcga tgatcaaac cttctcgga tgatattct 
      agtgttgag cagttacga gctgtacct ccaggagtt 
      accatatcc ggagaaagt tacttcaac caatcagct 
      tgttttgtt tgcgcgaca ttggtgtcc ggcaacgat 
      gacacttga gccgtttca ggaagttat tattgatac 
      tccgaccat gtccgaatt aaaaacgaa agtggccga 
      actttcgaa tattcccgt cgaaaatgt ccaagtagc 
      atttgtcaa aagtacatt tccttgcga tatgcattt 
      gttagatat acacactga gttggagtg ggatccgaa 
      cgtgacgca tttgtatca gtggcctct gcaggtgga 
      tgatggaac tgccttttt ctacagaga tgggcgcga 
      ggagctgaa agagttaac gtatgagga gaagaaaga 
      gattaacat aagggagaa cacgcgctt gggtcgcgt 
      ccccttgaa gagttgtgc cacgttgga gaagtcgtt 
      gcgtattta tttagataa ttggccttc tgaaacaga 
      ggacaattg tatagactg acatgccag taaacaata 
      caataattc gttacctta ctgcatcct aagctgaat 
      cgacgacta cgatgcgcc ggataagtt gtatggact 
      gttttaaat aatacaatg tttttttta gcggcggac 
      gtcgaaatg gcattgttg tattttaat taattttgt 
      aattgtttt tatgccatg cattcatgc tctcgattt 
      ttcattaat attgttatt tcgtttgtt tcatttata 
      tttatttgt tttaacgtt agacaatag tttggaaaa 
      gtttaaatt attgtacac ttttacttt atcgtacga 
      tttgtagtt tagtttttt tttttcgtt ttgtcggac 
      ttgtctgtt atcgtttat tactggcat ggttgaaac 
      cccatttta tagtttccc aaaatgaat tctggggca 
      gagccacct gaagacaaa cgttcattt tgaggaatt 
      cattttgct aacgcttgt cattataca gctactttg 
      taaaaatat ttatttgtg actgatcaa taaaatctt 
      tatctacag
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