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Gene information
General information
TC032637 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 27049729..27065086
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032637
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02737

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032637 RA
      MADLQDALP PGWESRTDF ISGKTYYIN HNTKTTTWD 
      DPRARQKHS TSKHVEYIP LQHGSPDLR RNYVYPSQI 
      SPIPAFQIS STQNPKCNA LQELRSSSR LSPLTVRGA 
      KVQDSSLTI QSETDDAVS KISAMFPTV SETHIRLLM 
      KKYHNREAL VISALQVEK NPITTPGPF ATPPPQRNI 
      HHPGVHALQ MTPPLGLRP SSRGGSPVL RPGSGGTNS 
      SGSPRVGDA FKGSPRPHS SPKLKLRYM KSIFPQADE 
      TVILDVLYN NESNIQKVS EILIDMGFN RKDTVKAAQ 
      QKMETKIEE KRIEEVKKN EPPPPPPKI KTKEEKAAL 
      KEEMKKKYA DVPEHLIKI ALESVDFNE SRAIQILEI 
      IVQEDTDKK AAESAASSS SEVQQEEVV AKSELKVTE 
      MPTSQSRQS IKSLLKADK IDKDKTAYS REDYVNWQG 
      PNSNHRKGP QALSRGPNR SLLSQRTYQ ACGSNPELR 
      KGPKQGLAK GSIFSQLKN VAVGGESRG K
    • >TC032637 RB
      MADLQDALP PGWESRTDF ISGKTYYIN HNTKTTTWD 
      DPRARQKHS TSKHVEYIP LQELRSSSR LSPLTVRGA 
      KVQDSSLTI QSETDDAVS KISAMFPTV SETHIRLLM 
      KKYHNREAL VISALQVEK NPITTPGPF ATPPPQRNI 
      HHPGVHALQ MTPPLGLRP SSRGGSPVL RPGSGGTNS 
      SGSPRVGDA FKGSPRPHS SPKLKLRYM KSIFPQADE 
      TVILDVLYN NESNIQKVS EILIDMGFN RKDTVKAAQ 
      QKMETKIEE KRIEEVKKN EPPPPPPKI KTKEEKAAL 
      KEEMKKKYA DVPEHLIKI ALESVDFNE SRAIQILEI 
      IVQEDTDKK AAESAASSS SEVQQEEVV AKSELKVTE 
      MPTSQSRQS IKSLLKADK IDKDKTAYS REDYVNWQG 
      PNSNHRKGP QALSRGPNR SLLSQRTYQ ACGSNPELR 
      KGPKQGLAK GSIFSQLKN VAVGGESRG K
    CDS sequence
    • >TC032637 RA
      atggcggat ctccaggac gcgttaccc cctggttgg 
      gagagcaga accgatttt atatctgga aaaacgtac 
      tacataaac cacaatacg aaaacaaca acgtgggat 
      gatccaaga gctcgccaa aagcactcg acctctaaa 
      catgttgag tacatccct ctccagcat ggatcacca 
      gatctgaga aggaattat gtttacccc agtcaaatt 
      tcccctata ccggctttt cagatctct tcaactcaa 
      aatcccaag tgtaatgcg ttgcaggaa cttcgcagc 
      agctcgcgc ctgagcccc ctcacagtc cggggggcc 
      aaagtccaa gacagctcc ctaaccatc caatcagag 
      accgacgat gccgtttca aaaatcagc gccatgttc 
      cccaccgtc tccgagacc cacatccgc cttttgatg 
      aaaaagtat cacaatcgg gaagctctt gtgataagt 
      gcgttacag gtggaaaaa aacccgatc acaacgcct 
      ggacctttc gcaactccc cctcctcag cgcaacatc 
      caccatcct ggcgtgcac gccttgcaa atgacgccc 
      ccccttggg ctgcgcccc agctcgagg ggcggcagt 
      ccggtctta aggcctgga tcggggggc accaacagc 
      tcgggctcc ccacgcgtg ggagacgcc ttcaagggg 
      tcgccgagg ccgcactct tctcccaaa ttaaaacta 
      aggtacatg aagtcgatt tttccacaa gctgacgag 
      acggttatt ttggacgta ctgtataat aacgaaagt 
      aacatacaa aaagtgtct gagattttg attgacatg 
      ggtttcaac cggaaagac accgtgaaa gcggctcaa 
      caaaaaatg gaaacaaaa atcgaagaa aagaggatt 
      gaagaagtg aagaaaaat gaaccaccg ccgcctccg 
      cccaagatt aagacaaag gaagaaaag gccgcactg 
      aaagaggaa atgaagaag aaatatgcc gacgtacct 
      gaacacttg attaaaatt gcgttagag agtgttgat 
      tttaatgag agtcgagcc attcagatt ctcgagatt 
      atagtacag gaagatact gataagaag gcggctgaa 
      agcgcagct agttctagt agcgaagtc caacaagag 
      gaagttgta gcaaaatct gaactcaaa gtaactgaa 
      atgccaact tcgcaaagt cgccaatcg attaaatcg 
      cttttgaag gccgataaa attgataag gataaaacc 
      gcatattcc agggaagac tacgtcaat tggcaaggg 
      cctaactca aaccaccgc aaaggcccc caagcccta 
      agtagaggc cctaaccgc tccctgctc tcgcaaaga 
      acctaccaa gcttgtggt tcaaaccca gaactgcgc 
      aaaggcccc aaacaaggc cttgccaaa ggcagcatt 
      ttctcccaa ttgaaaaat gtcgcagtt gggggcgag 
      tctcgggga aagtag
    • >TC032637 RB
      atggcggat ctccaggac gcgttaccc cctggttgg 
      gagagcaga accgatttt atatctgga aaaacgtac 
      tacataaac cacaatacg aaaacaaca acgtgggat 
      gatccaaga gctcgccaa aagcactcg acctctaaa 
      catgttgag tacatccct ctccaggaa cttcgcagc 
      agctcgcgc ctgagcccc ctcacagtc cggggggcc 
      aaagtccaa gacagctcc ctaaccatc caatcagag 
      accgacgat gccgtttca aaaatcagc gccatgttc 
      cccaccgtc tccgagacc cacatccgc cttttgatg 
      aaaaagtat cacaatcgg gaagctctt gtgataagt 
      gcgttacag gtggaaaaa aacccgatc acaacgcct 
      ggacctttc gcaactccc cctcctcag cgcaacatc 
      caccatcct ggcgtgcac gccttgcaa atgacgccc 
      ccccttggg ctgcgcccc agctcgagg ggcggcagt 
      ccggtctta aggcctgga tcggggggc accaacagc 
      tcgggctcc ccacgcgtg ggagacgcc ttcaagggg 
      tcgccgagg ccgcactct tctcccaaa ttaaaacta 
      aggtacatg aagtcgatt tttccacaa gctgacgag 
      acggttatt ttggacgta ctgtataat aacgaaagt 
      aacatacaa aaagtgtct gagattttg attgacatg 
      ggtttcaac cggaaagac accgtgaaa gcggctcaa 
      caaaaaatg gaaacaaaa atcgaagaa aagaggatt 
      gaagaagtg aagaaaaat gaaccaccg ccgcctccg 
      cccaagatt aagacaaag gaagaaaag gccgcactg 
      aaagaggaa atgaagaag aaatatgcc gacgtacct 
      gaacacttg attaaaatt gcgttagag agtgttgat 
      tttaatgag agtcgagcc attcagatt ctcgagatt 
      atagtacag gaagatact gataagaag gcggctgaa 
      agcgcagct agttctagt agcgaagtc caacaagag 
      gaagttgta gcaaaatct gaactcaaa gtaactgaa 
      atgccaact tcgcaaagt cgccaatcg attaaatcg 
      cttttgaag gccgataaa attgataag gataaaacc 
      gcatattcc agggaagac tacgtcaat tggcaaggg 
      cctaactca aaccaccgc aaaggcccc caagcccta 
      agtagaggc cctaaccgc tccctgctc tcgcaaaga 
      acctaccaa gcttgtggt tcaaaccca gaactgcgc 
      aaaggcccc aaacaaggc cttgccaaa ggcagcatt 
      ttctcccaa ttgaaaaat gtcgcagtt gggggcgag 
      tctcgggga aagtag
    mRNA sequence
    • >TC032637 RA
      ggccatctt aaattaact gtcatttgt gtttgtgta 
      tggtgttta tcgaatttt cacaatttt ttgtgtgag 
      gcaccaatg gcggatctc caggacgcg ttaccccct 
      ggttgggag agcagaacc gattttata tctggaaaa 
      acgtactac ataaaccac aatacgaaa acaacaacg 
      tgggatgat ccaagagct cgccaaaag cactcgacc 
      tctaaacat gttgagtac atccctctc cagcatgga 
      tcaccagat ctgagaagg aattatgtt taccccagt 
      caaatttcc cctataccg gcttttcag atctcttca 
      actcaaaat cccaagtgt aatgcgttg caggaactt 
      cgcagcagc tcgcgcctg agccccctc acagtccgg 
      ggggccaaa gtccaagac agctcccta accatccaa 
      tcagagacc gacgatgcc gtttcaaaa atcagcgcc 
      atgttcccc accgtctcc gagacccac atccgcctt 
      ttgatgaaa aagtatcac aatcgggaa gctcttgtg 
      ataagtgcg ttacaggtg gaaaaaaac ccgatcaca 
      acgcctgga cctttcgca actccccct cctcagcgc 
      aacatccac catcctggc gtgcacgcc ttgcaaatg 
      acgcccccc cttgggctg cgccccagc tcgaggggc 
      ggcagtccg gtcttaagg cctggatcg gggggcacc 
      aacagctcg ggctcccca cgcgtggga gacgccttc 
      aaggggtcg ccgaggccg cactcttct cccaaatta 
      aaactaagg tacatgaag tcgattttt ccacaagct 
      gacgagacg gttattttg gacgtactg tataataac 
      gaaagtaac atacaaaaa gtgtctgag attttgatt 
      gacatgggt ttcaaccgg aaagacacc gtgaaagcg 
      gctcaacaa aaaatggaa acaaaaatc gaagaaaag 
      aggattgaa gaagtgaag aaaaatgaa ccaccgccg 
      cctccgccc aagattaag acaaaggaa gaaaaggcc 
      gcactgaaa gaggaaatg aagaagaaa tatgccgac 
      gtacctgaa cacttgatt aaaattgcg ttagagagt 
      gttgatttt aatgagagt cgagccatt cagattctc 
      gagattata gtacaggaa gatactgat aagaaggcg 
      gctgaaagc gcagctagt tctagtagc gaagtccaa 
      caagaggaa gttgtagca aaatctgaa ctcaaagta 
      actgaaatg ccaacttcg caaagtcgc caatcgatt 
      aaatcgctt ttgaaggcc gataaaatt gataaggat 
      aaaaccgca tattccagg gaagactac gtcaattgg 
      caagggcct aactcaaac caccgcaaa ggcccccaa 
      gccctaagt agaggccct aaccgctcc ctgctctcg 
      caaagaacc taccaagct tgtggttca aacccagaa 
      ctgcgcaaa ggccccaaa caaggcctt gccaaaggc 
      agcattttc tcccaattg aaaaatgtc gcagttggg 
      ggcgagtct cggggaaag tagcagttc atgtgtact 
      attatttaa gtcgttgta aataagtgt gcatgcagt 
      gtttaaaaa aaaacggca aagcttgag gatattggc 
      atcgaatta ttcagattg atctgcaat ttcacaacc 
      aaagatttt gttacttgt acagtgttc acaaaagaa 
      cgtgcatca agagtgttg tatgaatac ggaggacta 
      taagttagg agacgattt ttttaaccc aaaaaaata 
      tgggacaga caaccattt t
    • >TC032637 RB
      ggccatctt aaattaact gtcatttgt gtttgtgta 
      tggtgttta tcgaatttt cacaatttt ttgtgtgag 
      gcaccaatg gcggatctc caggacgcg ttaccccct 
      ggttgggag agcagaacc gattttata tctggaaaa 
      acgtactac ataaaccac aatacgaaa acaacaacg 
      tgggatgat ccaagagct cgccaaaag cactcgacc 
      tctaaacat gttgagtac atccctctc caggaactt 
      cgcagcagc tcgcgcctg agccccctc acagtccgg 
      ggggccaaa gtccaagac agctcccta accatccaa 
      tcagagacc gacgatgcc gtttcaaaa atcagcgcc 
      atgttcccc accgtctcc gagacccac atccgcctt 
      ttgatgaaa aagtatcac aatcgggaa gctcttgtg 
      ataagtgcg ttacaggtg gaaaaaaac ccgatcaca 
      acgcctgga cctttcgca actccccct cctcagcgc 
      aacatccac catcctggc gtgcacgcc ttgcaaatg 
      acgcccccc cttgggctg cgccccagc tcgaggggc 
      ggcagtccg gtcttaagg cctggatcg gggggcacc 
      aacagctcg ggctcccca cgcgtggga gacgccttc 
      aaggggtcg ccgaggccg cactcttct cccaaatta 
      aaactaagg tacatgaag tcgattttt ccacaagct 
      gacgagacg gttattttg gacgtactg tataataac 
      gaaagtaac atacaaaaa gtgtctgag attttgatt 
      gacatgggt ttcaaccgg aaagacacc gtgaaagcg 
      gctcaacaa aaaatggaa acaaaaatc gaagaaaag 
      aggattgaa gaagtgaag aaaaatgaa ccaccgccg 
      cctccgccc aagattaag acaaaggaa gaaaaggcc 
      gcactgaaa gaggaaatg aagaagaaa tatgccgac 
      gtacctgaa cacttgatt aaaattgcg ttagagagt 
      gttgatttt aatgagagt cgagccatt cagattctc 
      gagattata gtacaggaa gatactgat aagaaggcg 
      gctgaaagc gcagctagt tctagtagc gaagtccaa 
      caagaggaa gttgtagca aaatctgaa ctcaaagta 
      actgaaatg ccaacttcg caaagtcgc caatcgatt 
      aaatcgctt ttgaaggcc gataaaatt gataaggat 
      aaaaccgca tattccagg gaagactac gtcaattgg 
      caagggcct aactcaaac caccgcaaa ggcccccaa 
      gccctaagt agaggccct aaccgctcc ctgctctcg 
      caaagaacc taccaagct tgtggttca aacccagaa 
      ctgcgcaaa ggccccaaa caaggcctt gccaaaggc 
      agcattttc tcccaattg aaaaatgtc gcagttggg 
      ggcgagtct cggggaaag tagcagttc atgtgtact 
      attatttaa gtcgttgta aataagtgt gcatgcagt 
      gtttaaaaa aaaacggca aagcttgag gatattggc 
      atcgaatta ttcagattg atctgcaat ttcacaacc 
      aaagatttt gttacttgt acagtgttc acaaaagaa 
      cgtgcatca agagtgttg tatgaatac ggaggacta 
      taagttagg agacgattt ttttaaccc aaaaaaata 
      tgggacaga caaccattt t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY