Status
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Gene information
General information
TC003900 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 26375855..26387149
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003900
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02735

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003900 RA
      MSVFNDFQQ VWLQRFPDN PLPGAWEEH VKLNLAKHR 
      QKVSMFKEE VRKEEIYVE YLERLLLDI QKHKKQESG 
      EEGESSKSD VTADNVSNS ATNLSLEEF ETFSANMCV 
      TEISNVLSK LNSEQSEDV DLSMPDPSS FVTVIEVNG 
      LKPSASTQT PPKIPVKPD FSRNNSLQE KIEPEERVS 
      TPEEPYYDV VAPEDQDFN LPESDNSSA ENTLSSQAS 
      FMPKKGSQS PSASNYVNI EYFIQASNQ NDNEHSSSD 
      EEGTEMRHR GSNGSYASS SSLDSSTPH SRRKFQPED 
      LETATENSR YKCILKNII SSEEEYVEW LSVSMKYMK 
      ALQATLTTS QRIITETEL NTIFYKIPE LYGVHSSFL 
      EVLKNHVEK NDNSGKTGE CFKNLALNL ELYGAFLSN 
      YGRALDTVR KCCETNSLF RDVTKNITC KYLSKQPNN 
      LEEILHKPV ARVQKNALV LHDLLNSLQ GTHPDYASL 
      SEALQLTQG FLDQFNMIQ TKSMFPVSD RAQRRLVKN 
      SFIVEFADG HRKLRHLFL FNDVIACAK YKPSGRTEK 
      YTFELKWFI PVEDIIIFR ETSPQHHHE STVPNIVSL 
      KSQASNVRD QLRQEEKAL EDKKTRSTR GSDKFKKRL 
      VELESQLVL VSPNLPFRI KNRQTGRLY TFFLSSEFE 
      RTQWVEAIL TLQKTGKPP VPSPSFSML ELQTWITAC 
      RAFLKTNMG SYLLRSVHD ESLLVGDLH VHIFELTGL 
      EYSCELYVV FEVDFYGHF FQKAKTKAV QSSNVFTWG 
      EKFVIDLEG CENLRILVY RTSTSTIKE DTLVGKYTQ 
      KLSRSWLRT EPVEKKFNI NVATLRLNL TYIPCVVSL 
      RRLPTGKNG ALFGEKIQV VCKRQKRDI PFIITACIK 
      EVERRGMTE VGLYRVSGS ASDISKLKK SFETNSYEA 
      EQLLKEVDI HSVTGILKL YLRELPEAL FTDQLYPAL 
      SEAFNQSNG NYTRRIELL KGCFAKLPQ QNKIIIKFI 
      LDHLIRVHK HEEDNKMSL HNLATVFGP TLLRPGFKA 
      SQNKPKDTL SAETVDVMA QAGILYCYL QDLDSNKNN 
      C
    CDS sequence
    • >TC003900 RA
      atgagtgtt tttaacgat tttcaacaa gtgtggttg 
      caacgattt cctgacaat ccacttcca ggggcatgg 
      gaggaacat gtcaagttg aatttggcg aaacatcga 
      cagaaagtg tcgatgttc aaggaagaa gtccgcaag 
      gaagagatt tacgttgag tatttggag cgtctgctc 
      ctcgatatc caaaaacac aagaaacaa gagtcggga 
      gaagaaggt gaatcaagc aagagtgat gtcacagct 
      gataacgta tcaaatagc gcgaccaac ttgagcctt 
      gaggagttc gagaccttt tcggcaaac atgtgcgtg 
      acggagatt tcgaacgta ttgtcgaaa ctcaactct 
      gagcaaagt gaagacgtt gatttgtca atgccagac 
      ccgagttct ttcgtgacg gtgatcgaa gtgaatggt 
      ttgaaaccg tcggcgagc actcaaact ccaccaaag 
      ataccagta aagcctgat tttagtaga aataacagt 
      ttgcaagag aaaatcgag cctgaagag cgcgtgtca 
      acacctgag gagccttac tacgacgtt gttgcgccc 
      gaagatcag gacttcaat ctgcctgaa agtgataat 
      tcaagtgct gaaaatact ctaagttcc caggcgtcc 
      tttatgccg aagaaaggg tcacaatca ccaagtgct 
      tcaaactac gtcaatatt gaatatttc atacaggcg 
      agcaatcaa aacgataat gaacacagt agcagtgac 
      gaggaaggc actgaaatg cgtcacaga ggcagcaac 
      ggctcgtac gcaagttca agttcatta gattcatca 
      acgccccac agcagacga aaatttcaa cctgaggat 
      cttgagact gccactgaa aactcacgg tacaaatgc 
      atactaaaa aatataata agcagcgag gaggagtac 
      gtggaatgg ttgagtgtg tcaatgaag tacatgaaa 
      gcgctccaa gcgactttg acgacgtcc caacgaatt 
      atcacagag actgaactg aacacaatt ttctacaaa 
      attccagaa ctgtacggt gtgcattca agcttcctc 
      gaagtactg aaaaatcac gtggaaaag aatgacaat 
      tcgggaaag acgggcgaa tgcttcaag aacttggct 
      ttaaatcta gaactgtac ggagcattt ttgagcaat 
      tatgggcgc gctttggac acggtgcga aaatgctgc 
      gagacgaat tcgctattt cgggacgtg accaaaaac 
      atcacctgc aaatacctc tcgaaacag ccgaacaac 
      ttggaggaa atcctacac aagccggtg gcaagggtg 
      caaaagaat gcgttggtt ttgcacgat ttgttgaat 
      tcgctgcaa ggaacacat cccgattac gctagcctt 
      tctgaagct ttacaattg acgcaaggt ttccttgac 
      cagtttaat atgattcaa accaagtcc atgtttcct 
      gtgtccgac cgagcgcaa aggcggctc gttaaaaat 
      tcatttatt gtcgaattt gcagacggc catcggaaa 
      ttgaggcat ttgttttta tttaatgac gtcatcgca 
      tgtgcgaaa tacaagcca tccgggcgt actgaaaaa 
      tacacattt gaactaaaa tggttcatc cctgtggaa 
      gacataatc attttccgc gaaaccagc cctcagcat 
      caccacgaa tcaactgta ccaaatatt gtaagtctt 
      aaatcgcaa gcatcaaat gttagagat caattacgc 
      caagaagaa aaagcttta gaagataag aaaacacgc 
      agcactcgt ggttccgac aaattcaaa aaacgtcta 
      gtggaactc gaaagtcag ttagtttta gtttcacca 
      aatttaccg ttccgtatt aaaaaccgt cagactgga 
      aggttgtac acgttcttt ttgtcgtcg gaattcgaa 
      cgcacccag tgggtcgaa gcgatttta acgctacag 
      aaaacgggt aaaccaccg gtaccttca cccagtttt 
      agcatgctg gagttgcaa acgtggata acggcatgt 
      cgggcattt ttaaagacg aatatgggg tcgtatctg 
      ttgagaagc gtccatgat gaaagcctc ctagtcggg 
      gatttacat gtgcatatt tttgaactg accggactt 
      gagtacagc tgtgagcta tacgtggtg tttgaggtc 
      gatttctac ggccacttt ttccaaaaa gccaaaact 
      aaggccgtt cagagctcg aacgtgttc acgtggggc 
      gagaaattc gtaattgac cttgaagga tgcgagaat 
      ttgcgtatt ttagtatat agaactagt acaagtaca 
      attaaggag gatacactg gtggggaaa tacacacag 
      aagttgagt cggagttgg ctgaggact gaaccagtt 
      gaaaagaag tttaatata aatgtggcc acactgagg 
      ctgaatttg acgtacatt ccgtgtgtg gtgagtcta 
      cggagattg ccgacgggg aaaaatggc gccctcttc 
      ggcgagaaa attcaagtc gtttgcaag cgccaaaaa 
      agagacatt ccgttcatt atcacagca tgtatcaag 
      gaagtggaa aggcgggga atgaccgaa gtgggtttg 
      taccgcgtt tcaggttcc gcatcggat atttccaag 
      ctgaaaaaa tcgttcgag acaaattct tacgaagcc 
      gaacaactg ttgaaagaa gtcgatatc cattccgta 
      accggtatt ttaaaatta tacctacgc gagcttccc 
      gaagctctt tttaccgat caactgtac ccagctttg 
      tcagaggca tttaaccaa agtaacgga aactacacc 
      cgtcgcatt gagctgttg aagggctgt ttcgccaag 
      cttccgcag caaaataaa atcattatc aagttcatt 
      ttggatcat ttgatccga gtgcataaa cacgaagag 
      gataataag atgtcgctg cacaatctg gcgacggtt 
      ttcgggccg acgctctta aggccgggc ttcaaagcc 
      agccagaac aagccgaag gatacgctt tcggccgaa 
      acggtggac gtaatggcg caagcgggg atcctgtat 
      tgctatttg caggacctt gattcgaac aaaaataat 
      tgctaa
    mRNA sequence
    • >TC003900 RA
      gataaatca acaccctgt tatgtcgtt gtgtcagtc 
      aattgtgtt tttgcttca tgttccagt agattagcg 
      taattaaat taaatgaag ggcttagtt aatatgaat 
      taatcaaaa atgagtgtt tttaacgat tttcaacaa 
      gtgtggttg caacgattt cctgacaat ccacttcca 
      ggggcatgg gaggaacat gtcaagttg aatttggcg 
      aaacatcga cagaaagtg tcgatgttc aaggaagaa 
      gtccgcaag gaagagatt tacgttgag tatttggag 
      cgtctgctc ctcgatatc caaaaacac aagaaacaa 
      gagtcggga gaagaaggt gaatcaagc aagagtgat 
      gtcacagct gataacgta tcaaatagc gcgaccaac 
      ttgagcctt gaggagttc gagaccttt tcggcaaac 
      atgtgcgtg acggagatt tcgaacgta ttgtcgaaa 
      ctcaactct gagcaaagt gaagacgtt gatttgtca 
      atgccagac ccgagttct ttcgtgacg gtgatcgaa 
      gtgaatggt ttgaaaccg tcggcgagc actcaaact 
      ccaccaaag ataccagta aagcctgat tttagtaga 
      aataacagt ttgcaagag aaaatcgag cctgaagag 
      cgcgtgtca acacctgag gagccttac tacgacgtt 
      gttgcgccc gaagatcag gacttcaat ctgcctgaa 
      agtgataat tcaagtgct gaaaatact ctaagttcc 
      caggcgtcc tttatgccg aagaaaggg tcacaatca 
      ccaagtgct tcaaactac gtcaatatt gaatatttc 
      atacaggcg agcaatcaa aacgataat gaacacagt 
      agcagtgac gaggaaggc actgaaatg cgtcacaga 
      ggcagcaac ggctcgtac gcaagttca agttcatta 
      gattcatca acgccccac agcagacga aaatttcaa 
      cctgaggat cttgagact gccactgaa aactcacgg 
      tacaaatgc atactaaaa aatataata agcagcgag 
      gaggagtac gtggaatgg ttgagtgtg tcaatgaag 
      tacatgaaa gcgctccaa gcgactttg acgacgtcc 
      caacgaatt atcacagag actgaactg aacacaatt 
      ttctacaaa attccagaa ctgtacggt gtgcattca 
      agcttcctc gaagtactg aaaaatcac gtggaaaag 
      aatgacaat tcgggaaag acgggcgaa tgcttcaag 
      aacttggct ttaaatcta gaactgtac ggagcattt 
      ttgagcaat tatgggcgc gctttggac acggtgcga 
      aaatgctgc gagacgaat tcgctattt cgggacgtg 
      accaaaaac atcacctgc aaatacctc tcgaaacag 
      ccgaacaac ttggaggaa atcctacac aagccggtg 
      gcaagggtg caaaagaat gcgttggtt ttgcacgat 
      ttgttgaat tcgctgcaa ggaacacat cccgattac 
      gctagcctt tctgaagct ttacaattg acgcaaggt 
      ttccttgac cagtttaat atgattcaa accaagtcc 
      atgtttcct gtgtccgac cgagcgcaa aggcggctc 
      gttaaaaat tcatttatt gtcgaattt gcagacggc 
      catcggaaa ttgaggcat ttgttttta tttaatgac 
      gtcatcgca tgtgcgaaa tacaagcca tccgggcgt 
      actgaaaaa tacacattt gaactaaaa tggttcatc 
      cctgtggaa gacataatc attttccgc gaaaccagc 
      cctcagcat caccacgaa tcaactgta ccaaatatt 
      gtaagtctt aaatcgcaa gcatcaaat gttagagat 
      caattacgc caagaagaa aaagcttta gaagataag 
      aaaacacgc agcactcgt ggttccgac aaattcaaa 
      aaacgtcta gtggaactc gaaagtcag ttagtttta 
      gtttcacca aatttaccg ttccgtatt aaaaaccgt 
      cagactgga aggttgtac acgttcttt ttgtcgtcg 
      gaattcgaa cgcacccag tgggtcgaa gcgatttta 
      acgctacag aaaacgggt aaaccaccg gtaccttca 
      cccagtttt agcatgctg gagttgcaa acgtggata 
      acggcatgt cgggcattt ttaaagacg aatatgggg 
      tcgtatctg ttgagaagc gtccatgat gaaagcctc 
      ctagtcggg gatttacat gtgcatatt tttgaactg 
      accggactt gagtacagc tgtgagcta tacgtggtg 
      tttgaggtc gatttctac ggccacttt ttccaaaaa 
      gccaaaact aaggccgtt cagagctcg aacgtgttc 
      acgtggggc gagaaattc gtaattgac cttgaagga 
      tgcgagaat ttgcgtatt ttagtatat agaactagt 
      acaagtaca attaaggag gatacactg gtggggaaa 
      tacacacag aagttgagt cggagttgg ctgaggact 
      gaaccagtt gaaaagaag tttaatata aatgtggcc 
      acactgagg ctgaatttg acgtacatt ccgtgtgtg 
      gtgagtcta cggagattg ccgacgggg aaaaatggc 
      gccctcttc ggcgagaaa attcaagtc gtttgcaag 
      cgccaaaaa agagacatt ccgttcatt atcacagca 
      tgtatcaag gaagtggaa aggcgggga atgaccgaa 
      gtgggtttg taccgcgtt tcaggttcc gcatcggat 
      atttccaag ctgaaaaaa tcgttcgag acaaattct 
      tacgaagcc gaacaactg ttgaaagaa gtcgatatc 
      cattccgta accggtatt ttaaaatta tacctacgc 
      gagcttccc gaagctctt tttaccgat caactgtac 
      ccagctttg tcagaggca tttaaccaa agtaacgga 
      aactacacc cgtcgcatt gagctgttg aagggctgt 
      ttcgccaag cttccgcag caaaataaa atcattatc 
      aagttcatt ttggatcat ttgatccga gtgcataaa 
      cacgaagag gataataag atgtcgctg cacaatctg 
      gcgacggtt ttcgggccg acgctctta aggccgggc 
      ttcaaagcc agccagaac aagccgaag gatacgctt 
      tcggccgaa acggtggac gtaatggcg caagcgggg 
      atcctgtat tgctatttg caggacctt gattcgaac 
      aaaaataat tgctaaaat tatgtatat aaataccag 
      tattgtatt cgtatcgtg tgattttgc ggtcactga 
      gacttatat ttatgtgaa aagtatttt tgtatagtg 
      gcttttccg aatgaattt tgtatccta ttccgaagc 
      ttttgtaaa taaatatag actatcaga caaatggtg 
      gcgcattat cgaaatcct ggttaagtc agattttac 
      ttttttgtg attatttat ttggaattt taatgtgcg 
      aagaacgca atattagtg aaatatga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY