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Gene information
General information
TC032628
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003896
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid25: 910644..917968
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032628
    iBeetle screen iB_02734
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032628 RA
      MDSSKFSMF FGNVPTFTI PGRTFPVEI LFSKNPVED 
      YVDAAVKQA LQIHLQPPS GDILIFMPG QEDIEVTCE 
      VLAERLAEI ENAPELSIL PIYSQLPSD LQAKIFQRS 
      PEGIRKCVV ATNIAETSL TVDGIIFVI DSGYCKLKV 
      YNPRIGMDA LQIYPISQA NANQRSGRA GRTGPGQAF 
      RLYTERQYK EELLVTTVP EIQRTNLAN TVLLLKSLG 
      VQDLLQFHF MDPPPQDNI LNSLYQLWI LGALDHTGV 
      LTKLGRQMA EFPLDPPQC QMLIVSSQM GCTAEILII 
      VSMLSVPSI FYRPKGREE EADGVREKF QVPESDHLT 
      YLNVYNQWK QNKYSSHWC NEHFIHIKA MRKVREVRQ 
      QLKDILVQQ KLEIKSCGT DWDIVRKCI CSAYFHQAA 
      RLKGIGEYV NCRTGMPCH LHPTSALFG LGSTPDYVV 
      YHELVMTAR EYMQCVTAV DGHWLAELG PMFFSLKET 
      GKSGRAKKK QAAEHLQEM ENQMQVAQE EMRARKEAA 
      DKREAAMNK GQEIVSAGA TPRRTPARF GL
    CDS sequence
    • >TC032628 RA
      atggactcg agcaaattc tcgatgttt ttcggcaac 
      gtgcccacc ttcacaatt cctggtcgc acattccct 
      gtggaaatc ctcttcagt aaaaatcca gtggaggat 
      tatgtggac gctgccgtc aagcaggct ctccaaatc 
      cacctccaa cctccgtct ggagatatc cttattttt 
      atgcccggt caggaggac attgaagtg acgtgtgaa 
      gtcctggct gagagactt gcagagatt gaaaatgct 
      ccagaattg tcaattttg ccgatttat tcacaacta 
      ccgtccgat ctacaagcg aaaattttt caacggtca 
      ccggaaggg attaggaag tgtgtggtg gcgacgaat 
      attgcggaa acgtcgttg actgtcgat gggattatt 
      tttgtcatc gattcgggt tattgcaag ttgaaggtt 
      tataatcct aggattggt atggatgcg ctccagatt 
      tacccaata agtcaagcc aatgccaat caaaggtcg 
      ggaagggcg ggccgtact ggtccaggt caggctttc 
      cgactctac accgaaagg caatacaaa gaagaattg 
      ctcgtaacg actgtacct gaaattcag cggacgaat 
      ttagccaat acggtcctg ctgttaaaa tcgttggga 
      gtacaagat ttgctccaa tttcatttc atggaccct 
      ccgcctcaa gataatatt ctcaattct ttgtaccaa 
      ttatggatt ttgggcgcc cttgatcat accggcgtc 
      ctgacaaaa ctcggccga caaatggcc gaatttcct 
      ctagatcca cctcaatgt cagatgttg attgtttca 
      agtcagatg gggtgcact gccgaaatt ttgataata 
      gtatcaatg ttgtcagtg ccgtccatt ttctatagg 
      cctaaagga cgcgaggag gaagccgat ggagtcaga 
      gagaagttc caagttccg gaaagtgac catttgact 
      taccttaac gtttacaac cagtggaaa caaaataaa 
      tattcatcg cattggtgc aacgagcat tttattcac 
      atcaaagcg atgcgtaaa gtgagggaa gtgagacaa 
      cagttgaag gatatccta gtgcaacaa aaactggag 
      attaagtcg tgtggcacc gactgggat atcgtcaga 
      aaatgcatt tgctcggct tatttccac caagcagcg 
      cgcttgaag ggcatcggc gagtacgtc aattgccgt 
      acgggcatg ccctgccac ctgcacccc acatcggcc 
      cttttcggc ctcgggagc actcccgac tacgtcgtc 
      taccacgag ctggtgatg acagcccgc gagtacatg 
      cagtgtgtc actgcggtg gacggccat tggttggcc 
      gaactaggc ccaatgttc ttctcactg aaagaaacc 
      gggaaaagt ggccgggcg aagaagaaa caagccgcc 
      gagcacctc caggagatg gagaaccag atgcaggtg 
      gcccaggag gagatgagg gcccgcaag gaagcggcg 
      gacaagcga gaggcggcc atgaacaag ggccaggag 
      attgtttca gccggggcg acgccgaga aggacaccg 
      gcccggttt ggtttataa
    mRNA sequence
    • >TC032628 RA
      tggcgctta taccactgg gccaccggg ccccagatt 
      gtggctcgc cgtcacgac ctcaaactc atcgtcacc 
      tccgccacg atggactcg agcaaattc tcgatgttt 
      ttcggcaac gtgcccacc ttcacaatt cctggtcgc 
      acattccct gtggaaatc ctcttcagt aaaaatcca 
      gtggaggat tatgtggac gctgccgtc aagcaggct 
      ctccaaatc cacctccaa cctccgtct ggagatatc 
      cttattttt atgcccggt caggaggac attgaagtg 
      acgtgtgaa gtcctggct gagagactt gcagagatt 
      gaaaatgct ccagaattg tcaattttg ccgatttat 
      tcacaacta ccgtccgat ctacaagcg aaaattttt 
      caacggtca ccggaaggg attaggaag tgtgtggtg 
      gcgacgaat attgcggaa acgtcgttg actgtcgat 
      gggattatt tttgtcatc gattcgggt tattgcaag 
      ttgaaggtt tataatcct aggattggt atggatgcg 
      ctccagatt tacccaata agtcaagcc aatgccaat 
      caaaggtcg ggaagggcg ggccgtact ggtccaggt 
      caggctttc cgactctac accgaaagg caatacaaa 
      gaagaattg ctcgtaacg actgtacct gaaattcag 
      cggacgaat ttagccaat acggtcctg ctgttaaaa 
      tcgttggga gtacaagat ttgctccaa tttcatttc 
      atggaccct ccgcctcaa gataatatt ctcaattct 
      ttgtaccaa ttatggatt ttgggcgcc cttgatcat 
      accggcgtc ctgacaaaa ctcggccga caaatggcc 
      gaatttcct ctagatcca cctcaatgt cagatgttg 
      attgtttca agtcagatg gggtgcact gccgaaatt 
      ttgataata gtatcaatg ttgtcagtg ccgtccatt 
      ttctatagg cctaaagga cgcgaggag gaagccgat 
      ggagtcaga gagaagttc caagttccg gaaagtgac 
      catttgact taccttaac gtttacaac cagtggaaa 
      caaaataaa tattcatcg cattggtgc aacgagcat 
      tttattcac atcaaagcg atgcgtaaa gtgagggaa 
      gtgagacaa cagttgaag gatatccta gtgcaacaa 
      aaactggag attaagtcg tgtggcacc gactgggat 
      atcgtcaga aaatgcatt tgctcggct tatttccac 
      caagcagcg cgcttgaag ggcatcggc gagtacgtc 
      aattgccgt acgggcatg ccctgccac ctgcacccc 
      acatcggcc cttttcggc ctcgggagc actcccgac 
      tacgtcgtc taccacgag ctggtgatg acagcccgc 
      gagtacatg cagtgtgtc actgcggtg gacggccat 
      tggttggcc gaactaggc ccaatgttc ttctcactg 
      aaagaaacc gggaaaagt ggccgggcg aagaagaaa 
      caagccgcc gagcacctc caggagatg gagaaccag 
      atgcaggtg gcccaggag gagatgagg gcccgcaag 
      gaagcggcg gacaagcga gaggcggcc atgaacaag 
      ggccaggag attgtttca gccggggcg acgccgaga 
      aggacaccg gcccggttt ggtttataa ctttttatt 
      tatttttgt aagaacaat aaaaaaaat attaatcaa 
      aaatgcttg tctggtaat tgttgacaa cccgttgaa 
      catttccaa aagcaggtg gagaggaag tcgaggacg 
      tggttgagg attgagggt tcgatgcgc gtgcgatta 
      ctaatccgg actgaaaat tgctaatta tttttaggt 
      attttttta agggtgtgg tacgtctgg atgggcgac 
      gtttgcagt aaatgtctt cgatgggtc cgtccaggc 
      ttgacactg aggataact cgctgataa cgctgtttt 
      tgtcgaagt tcatgtcaa gggaacatg tggaacagt 
      tgggggtga taactggag tttctggaa aatttggtg 
      agtcgttat acttaaata atgcgttta ccgtctagt 
      tcatcactt tgtgggctt ggccatgag aaactatcg 
      tcgtgtttg agaaaagtt atttcgatt gtttcggga 
      ctgggcgtt tttttcgct cgaaaattc attaagtct 
      gagttttgt gaaaatcaa ctgacagaa taattttgt 
      attttgaac