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Gene information
General information
TC031067
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003896
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid25: 892684..900198
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031067
    iBeetle screen iB_02733
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: -1.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: -1.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031067 RA
      MESEENLHR LEGISDQKG GLIVKKKPP TFKVPQPSL 
      LGLDRLAAA KRREKEEAA RKMSFTMDD NDNTDDSSS 
      LLKEKHSKD SRKFRSPHN ETPTYTGGI SDEARERLI 
      ERLKSNKQK EKGVYATTK DRHRDRDRD RERDKDRDR 
      GRHRDRESS HRSKTPRFR DEPKTPNLG HKDEISKSS 
      WDDDDDVGP SKKSSWDFP TPSTYKGSG GDWSERSTK 
      SRKYDESKR SSRESKRRK YEDESARFT PAHKYNSWA 
      KDRKRSGAT PMPGKDGVI KWDNTVDRE LWEEEQKRI 
      DREWYNMDE GYDDGNNPF SSVSEEYTK KKEEQLEQR 
      KKKRLSAQQ RQINKDNEL WERNRMLTS GAVHSIDFN 
      EDYDEESID RVHLLVHNI VPPFLDGRI VFTKQPEPV 
      IPVRDPTSD MAIVSRKGS HLVRVYREQ KERKKAQKK 
      HWELGGTKI GNIMGIKKK EDEEDKRFN KEDDTADYK 
      TDQKFAEHM KSTEASSDF AKKKTILEQ RRYLPVFAV 
      RQELLNVIR ENSVVIIVG ETGSGKTTQ LTQYLHEDG 
      YSKYGMIGC TQPRRVAAM SVAKRVSDE MGTQLGDDV 
      GYAIRFEDC TSENTVIKY MTDGILLRE SLREPDLDH 
      YSAVIMDEA HERSLSTDV LFGLLREVR KDSLIQSSF 
      FI
    CDS sequence
    • >TC031067 RA
      atggaatct gaggagaat ttgcaccgc ctggaaggc 
      ataagtgac caaaaaggc ggcctgatt gtaaaaaag 
      aagccccca acattcaaa gtgccccaa ccttcgctc 
      ctaggcctc gaccgtcta gcggcggca aagcgtcgc 
      gaaaaggag gaagcagca cgaaaaatg tcttttaca 
      atggacgat aatgacaac accgacgat tcaagttca 
      ctcttgaag gaaaaacac tcaaaagac agtcgcaaa 
      ttccggtca ccccacaac gagaccccc acgtacact 
      ggggggatc agtgacgag gcccgcgag cgcctcatc 
      gagcgtctc aagtcgaac aaacagaag gagaagggg 
      gtttacgcg acgactaaa gacagacac agggatagg 
      gatagggac cgggagagg gataaggac agggacagg 
      gggcgccac cgagacagg gagagttcg catcgttct 
      aagacccca cggtttcga gacgagccc aagaccccg 
      aatttggga cacaaggac gagatttca aagtcgtca 
      tgggatgac gatgatgat gtcgggccc tcgaaaaag 
      tcttcgtgg gatttcccg acgccttcg acttacaaa 
      gggagcggt ggggattgg tcggagcgg agcaccaag 
      tcacggaag tacgacgag tcgaagcga agctcgcgc 
      gagagtaaa cgaaggaaa tacgaggat gagagtgcg 
      aggtttacg ccggcccat aaatacaat tcctgggcc 
      aaagacagg aaacggagt ggggctacg cccatgcct 
      ggcaaagat ggggttatc aagtgggat aacaccgtc 
      gatagggaa ttatgggag gaggaacag aagaggatt 
      gacagggaa tggtacaat atggatgag gggtacgat 
      gatggaaat aaccctttt tcgagcgtc agtgaggaa 
      tatacgaag aaaaaagaa gaacagttg gagcaaagg 
      aagaagaag cggctttca gcacaacaa agacagatc 
      aataaggat aatgagttg tgggagagg aacagaatg 
      ctgacttcg ggggctgta cattccatt gactttaat 
      gaagactac gatgaagaa tccatcgat cgggtccat 
      ctgttggtg cacaatatt gtgccccct tttctggac 
      gggaggatt gtcttcacc aaacagccg gaaccggtc 
      attcctgtt agggacccc acttctgac atggccata 
      gtctcgcgc aaaggctcg caccttgtg cgggtgtac 
      cgtgagcag aaggagcgc aaaaaggct cagaagaag 
      cattgggag cttggaggc accaaaatc ggcaacatc 
      atgggaatc aagaaaaag gaggacgaa gaagataaa 
      cgattcaac aaagaggac gacacggct gattacaaa 
      accgatcag aaattcgcc gaacatatg aaatcgaca 
      gaagcctcc agtgatttc gccaagaag aagactatt 
      ctcgaacag agacgatat ttgcccgtt tttgccgtc 
      cgacaagaa ttgctgaat gttatcaga gagaattcg 
      gttgttatt attgtgggg gagaccgga agcgggaag 
      accacccag ctgacccaa tacttgcac gaggatggg 
      tatagcaaa tatgggatg attggatgc acccaaccg 
      agaagagtt gcagctatg tctgtagcc aaacgtgtg 
      agtgacgaa atggggacc caattaggg gacgacgtc 
      ggttacgcc atccgtttc gaagactgc acttctgaa 
      aacacagta atcaaatac atgactgat ggtattttg 
      ctgagagag agcctccga gagcccgac ttggaccac 
      tacagtgcc gtaattatg gacgaggct cacgagagg 
      tcgctcagt accgacgtc ctttttggc ttgctgaga 
      gaggtaagg aaagacagt ttaatccaa tcgtcattt 
      tttatttaa
    mRNA sequence
    • >TC031067 RA
      gtgaaaaac tcaggttat gtgagttta agagtgagg 
      ttatattta cataacatt tttttgcgt tattaaaaa 
      agaatggaa tctgaggag aatttgcac cgcctggaa 
      ggcataagt gaccaaaaa ggcggcctg attgtaaaa 
      aagaagccc ccaacattc aaagtgccc caaccttcg 
      ctcctaggc ctcgaccgt ctagcggcg gcaaagcgt 
      cgcgaaaag gaggaagca gcacgaaaa atgtctttt 
      acaatggac gataatgac aacaccgac gattcaagt 
      tcactcttg aaggaaaaa cactcaaaa gacagtcgc 
      aaattccgg tcaccccac aacgagacc cccacgtac 
      actgggggg atcagtgac gaggcccgc gagcgcctc 
      atcgagcgt ctcaagtcg aacaaacag aaggagaag 
      ggggtttac gcgacgact aaagacaga cacagggat 
      agggatagg gaccgggag agggataag gacagggac 
      agggggcgc caccgagac agggagagt tcgcatcgt 
      tctaagacc ccacggttt cgagacgag cccaagacc 
      ccgaatttg ggacacaag gacgagatt tcaaagtcg 
      tcatgggat gacgatgat gatgtcggg ccctcgaaa 
      aagtcttcg tgggatttc ccgacgcct tcgacttac 
      aaagggagc ggtggggat tggtcggag cggagcacc 
      aagtcacgg aagtacgac gagtcgaag cgaagctcg 
      cgcgagagt aaacgaagg aaatacgag gatgagagt 
      gcgaggttt acgccggcc cataaatac aattcctgg 
      gccaaagac aggaaacgg agtggggct acgcccatg 
      cctggcaaa gatggggtt atcaagtgg gataacacc 
      gtcgatagg gaattatgg gaggaggaa cagaagagg 
      attgacagg gaatggtac aatatggat gaggggtac 
      gatgatgga aataaccct ttttcgagc gtcagtgag 
      gaatatacg aagaaaaaa gaagaacag ttggagcaa 
      aggaagaag aagcggctt tcagcacaa caaagacag 
      atcaataag gataatgag ttgtgggag aggaacaga 
      atgctgact tcgggggct gtacattcc attgacttt 
      aatgaagac tacgatgaa gaatccatc gatcgggtc 
      catctgttg gtgcacaat attgtgccc ccttttctg 
      gacgggagg attgtcttc accaaacag ccggaaccg 
      gtcattcct gttagggac cccacttct gacatggcc 
      atagtctcg cgcaaaggc tcgcacctt gtgcgggtg 
      taccgtgag cagaaggag cgcaaaaag gctcagaag 
      aagcattgg gagcttgga ggcaccaaa atcggcaac 
      atcatggga atcaagaaa aaggaggac gaagaagat 
      aaacgattc aacaaagag gacgacacg gctgattac 
      aaaaccgat cagaaattc gccgaacat atgaaatcg 
      acagaagcc tccagtgat ttcgccaag aagaagact 
      attctcgaa cagagacga tatttgccc gtttttgcc 
      gtccgacaa gaattgctg aatgttatc agagagaat 
      tcggttgtt attattgtg ggggagacc ggaagcggg 
      aagaccacc cagctgacc caatacttg cacgaggat 
      gggtatagc aaatatggg atgattgga tgcacccaa 
      ccgagaaga gttgcagct atgtctgta gccaaacgt 
      gtgagtgac gaaatgggg acccaatta ggggacgac 
      gtcggttac gccatccgt ttcgaagac tgcacttct 
      gaaaacaca gtaatcaaa tacatgact gatggtatt 
      ttgctgaga gagagcctc cgagagccc gacttggac 
      cactacagt gccgtaatt atggacgag gctcacgag 
      aggtcgctc agtaccgac gtccttttt ggcttgctg 
      agagaggta aggaaagac agtttaatc caatcgtca 
      ttttttatt taaacctaa ctacaactt ttataaaaa 
      ataagtcgt tacaagtcg atataaaag aaataaata 
      acagtctta aat