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Gene information
General information
TC032612
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002116
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid22: 648306..659394
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032612
    iBeetle screen iB_02732
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 12.5% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    pronotum surface irregular - Number of animals showing the phenotype: 7


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032612 RA
      MTKSKITLY TSPTQKLII YIDPEKQGQ EACLCKIVN 
      SQNKFSPEE ALQSIEPLP FSEVTSEEQ LASFASSVS 
      TVDEFIPPP QLIERPKQL TPQESNSED YISSTETPE 
      NESSQQVND NVDENETSA KMPSRGKTQ KINFNLETP 
      ESSVLSLKP HNRRLSINS THSKSGNRI EVDESFIES 
      VACIIEANN LETRKEKLD ELLNNISGV KVGELSKAK 
      DEAKLGCWP SFKFSKKRK VAITPREPT PQRVASVPY 
      FNRSFYRYS LKSRIEGLT FQPHRVYRY GEQGSGFQA 
      KKGSQDFYE LRDQCLQRG ELFEDPEFP PTDSSLFYS 
      HRPDRRFEW RRPGEIVDN PQLFVEGFS RFDVQQGEL 
      GDCWLLAAV ANLTLYRRL FFQIVPDDQ GFDENYAGI 
      FHFRFWQYG RWIDVVIDD RLPTYRGEL VFLHSTEAN 
      EFWSALLEK AYAKLHGSY EALKGGSTC EAMEDFTGG 
      VTEMYELDA APPNLFKII SKAYERWSL MGCSIEPDP 
      DVLEAQTPE GLIRGHAYS ITKVQYVNI TTPNVSGKI 
      PLLRLRNPW GNESEWNGA WSDHSPEWH YISDSDKEE 
      LGLNFDADG EFWMSFKDF QQHFNRIEI CNLNPDSLA 
      EDELTEGRN KKWVMSVFE GEWVRGVTA GGCRNFLDT 
      FSHNPQYRV TLDEVDEGD DDDKCTLIV ALMQKNRRQ 
      MRRSVGPDL LTIGFAIYY LPYPDRAPK PLDLNFFKY 
      NASVARSPS FINLREVSC RFKLPRGTY CIVPSTFDP 
      NEEGEFLLR VFSEHQNNM EYDGSCSDS LPASVRDNE 
      VDDEDEENR RPRNPYPSY PSAPSHPYP DAPPPQPYS 
      TPYPPASPY PNNPYPYPQ SSYGQYPYP QNPQPYPPS 
      YPQYPQYPS EPDRSSRGS RGSDPITNF FNKLFR
    CDS sequence
    • >TC032612 RA
      atgacgaaa tctaagatc accttgtat acgtcgcca 
      acacaaaag ttaataatc tacattgat cccgaaaag 
      caaggacaa gaagcgtgt ttgtgtaaa attgtaaat 
      tcccaaaat aaattttca ccagaagag gcactacaa 
      tctatagaa cctttaccc ttttctgaa gttacttct 
      gaggaacag ctagcatct tttgcttct tctgtatca 
      acagtggat gaatttatt cctccacct cagctaatt 
      gaaagacct aaacaacta actccacaa gaatcaaat 
      tcagaagat tatatttca agtactgaa acgcctgaa 
      aacgaatct tcgcaacaa gtaaacgac aatgttgat 
      gaaaacgaa acaagcgca aaaatgcct agccgtgga 
      aaaacgcag aaaataaat ttcaacctt gaaacacct 
      gaaagctca gttttatca ctaaaaccg cacaaccga 
      cgtctttcc ataaactca actcacagc aaatcagga 
      aatagaatc gaagtcgat gaaagtttc atcgaaagt 
      gttgcttgc atcattgaa gcgaataat ttggaaact 
      cgcaaagag aaactggac gaacttttg aacaatata 
      agtggtgtt aaagtcggg gagttgtct aaagcaaaa 
      gacgaagca aaattgggt tgttggcct agctttaaa 
      ttttccaaa aagagaaaa gttgctata actccacga 
      gaaccaacg ccgcaacgt gtagcttct gttccttat 
      tttaatcgt tctttttac cgctactct ctgaaatcg 
      aggatcgaa ggactcact tttcagccc catagagtg 
      tacagatat ggcgaacaa gggtcaggt tttcaagca 
      aagaaagga tcgcaagat ttttacgaa ttacgcgac 
      caatgtcta caaagaggc gaattattt gaagacccc 
      gaatttcct ccaactgat tcttccctt ttctatagt 
      cacagacca gaccgacgt ttcgaatgg agacgtcca 
      ggggaaatt gtcgacaat ccccaacta ttcgtggag 
      ggtttttca cgctttgat gtgcaacaa ggcgaactg 
      ggcgattgt tggctactt gcagctgtt gctaattta 
      acactttat cgcaggctt tttttccaa attgttcca 
      gacgatcaa ggttttgat gagaattat gctgggatt 
      ttccatttc agattctgg cagtatgga cgctggatt 
      gatgttgtt attgatgat cgtttgcct acatacaga 
      ggcgaattg gtgtttttg cactctact gaagccaac 
      gaattttgg agcgcttta ttagagaaa gcatatgct 
      aagttgcat gggtcttat gaggcactc aaaggaggt 
      tcaacttgt gaagctatg gaagatttt actggtggt 
      gttacagaa atgtacgaa ttggacgca gctcctccg 
      aatttgttc aaaattatt tcgaaagct tacgaacgg 
      tggtctctg atgggttgt tccattgag cctgaccct 
      gacgtgctt gaagctcaa acccccgaa ggtctaatt 
      cgaggtcac gcctacagt atcacaaaa gtgcaatat 
      gttaacata acaacccct aatgtttct ggaaaaata 
      cctttactg agacttaga aatccatgg ggtaatgaa 
      tcagaatgg aatggggct tggagtgat cattctccc 
      gaatggcat tacatttcg gattcggat aaagaagaa 
      ctgggtttg aattttgat gccgatggt gaattctgg 
      atgtcgttt aaagatttc cagcaacat ttcaatcga 
      atagaaatc tgtaatttg aatccagat tcactggca 
      gaggatgaa ttgacggaa gggcgtaat aaaaagtgg 
      gtcatgtcg gtgtttgaa ggggagtgg gtgagggga 
      gtcactgca ggcggttgt cgcaacttt ttagacacc 
      ttttctcac aaccctcaa tatcgcgtt accttggac 
      gaagtcgat gagggcgat gacgacgac aagtgtacc 
      ttgattgtt gccctcatg cagaaaaat cgcagacaa 
      atgcgtcgc agtgttgga ccagattta cttactatt 
      ggatttgca atttattat cttccctac cctgataga 
      gctcccaaa cccctcgac ctcaacttc ttcaaatac 
      aacgcttcc gtagctaga tcacccagt tttataaac 
      ttgcgtgaa gtaagctgt cgctttaag ctccctcgt 
      ggtacctac tgcattgtg ccttcgaca tttgacccc 
      aacgaggaa ggagagttc cttctgcgt gtcttctcc 
      gaacatcaa aacaacatg gagtatgac ggttcttgt 
      tccgatagt ttgcctgct agtgttagg gataatgag 
      gttgatgac gaagatgaa gaaaatcgt cgacctcgt 
      aatccctat ccgagttac cccagcgct ccttcacat 
      ccctatcca gacgctcca cccccacag ccatactcc 
      actccctat ccgccggca agcccatat cctaataat 
      ccctatccc taccctcag agttcatac ggacaatat 
      ccctacccg caaaatccc caaccctat ccaccctca 
      taccctcag tacccgcaa tatccctca gaacctgac 
      agaagttca cgtgggtct agaggttca gacccgatt 
      acaaacttt ttcaataag ctgtttcgt taa
    mRNA sequence
    • >TC032612 RA
      aaatatttt aaatgtatt ttacaaaat taaaagtat 
      gaaacgact taaacaaat ctatgacga aatctaaga 
      tcaccttgt atacgtcgc caacacaaa agttaataa 
      tctacattg atcccgaaa agcaaggac aagaagcgt 
      gtttgtgta aaattgtaa attcccaaa ataaatttt 
      caccagaag aggcactac aatctatag aacctttac 
      ccttttctg aagttactt ctgaggaac agctagcat 
      cttttgctt cttctgtat caacagtgg atgaattta 
      ttcctccac ctcagctaa ttgaaagac ctaaacaac 
      taactccac aagaatcaa attcagaag attatattt 
      caagtactg aaacgcctg aaaacgaat cttcgcaac 
      aagtaaacg acaatgttg atgaaaacg aaacaagcg 
      caaaaatgc ctagccgtg gaaaaacgc agaaaataa 
      atttcaacc ttgaaacac ctgaaagct cagttttat 
      cactaaaac cgcacaacc gacgtcttt ccataaact 
      caactcaca gcaaatcag gaaatagaa tcgaagtcg 
      atgaaagtt tcatcgaaa gtgttgctt gcatcattg 
      aagcgaata atttggaaa ctcgcaaag agaaactgg 
      acgaacttt tgaacaata taagtggtg ttaaagtcg 
      gggagttgt ctaaagcaa aagacgaag caaaattgg 
      gttgttggc ctagcttta aattttcca aaaagagaa 
      aagttgcta taactccac gagaaccaa cgccgcaac 
      gtgtagctt ctgttcctt attttaatc gttcttttt 
      accgctact ctctgaaat cgaggatcg aaggactca 
      cttttcagc cccatagag tgtacagat atggcgaac 
      aagggtcag gttttcaag caaagaaag gatcgcaag 
      atttttacg aattacgcg accaatgtc tacaaagag 
      gcgaattat ttgaagacc ccgaatttc ctccaactg 
      attcttccc ttttctata gtcacagac cagaccgac 
      gtttcgaat ggagacgtc caggggaaa ttgtcgaca 
      atccccaac tattcgtgg agggttttt cacgctttg 
      atgtgcaac aaggcgaac tgggcgatt gttggctac 
      ttgcagctg ttgctaatt taacacttt atcgcaggc 
      tttttttcc aaattgttc cagacgatc aaggttttg 
      atgagaatt atgctggga ttttccatt tcagattct 
      ggcagtatg gacgctgga ttgatgttg ttattgatg 
      atcgtttgc ctacataca gaggcgaat tggtgtttt 
      tgcactcta ctgaagcca acgaatttt ggagcgctt 
      tattagaga aagcatatg ctaagttgc atgggtctt 
      atgaggcac tcaaaggag gttcaactt gtgaagcta 
      tggaagatt ttactggtg gtgttacag aaatgtacg 
      aattggacg cagctcctc cgaatttgt tcaaaatta 
      tttcgaaag cttacgaac ggtggtctc tgatgggtt 
      gttccattg agcctgacc ctgacgtgc ttgaagctc 
      aaacccccg aaggtctaa ttcgaggtc acgcctaca 
      gtatcacaa aagtgcaat atgttaaca taacaaccc 
      ctaatgttt ctggaaaaa tacctttac tgagactta 
      gaaatccat ggggtaatg aatcagaat ggaatgggg 
      cttggagtg atcattctc ccgaatggc attacattt 
      cggattcgg ataaagaag aactgggtt tgaattttg 
      atgccgatg gtgaattct ggatgtcgt ttaaagatt 
      tccagcaac atttcaatc gaatagaaa tctgtaatt 
      tgaatccag attcactgg cagaggatg aattgacgg 
      aagggcgta ataaaaagt gggtcatgt cggtgtttg 
      aaggggagt gggtgaggg gagtcactg caggcggtt 
      gtcgcaact ttttagaca ccttttctc acaaccctc 
      aatatcgcg ttaccttgg acgaagtcg atgagggcg 
      atgacgacg acaagtgta ccttgattg ttgccctca 
      tgcagaaaa atcgcagac aaatgcgtc gcagtgttg 
      gaccagatt tacttacta ttggatttg caatttatt 
      atcttccct accctgata gagctccca aacccctcg 
      acctcaact tcttcaaat acaacgctt ccgtagcta 
      gatcaccca gttttataa acttgcgtg aagtaagct 
      gtcgcttta agctccctc gtggtacct actgcattg 
      tgccttcga catttgacc ccaacgagg aaggagagt 
      tccttctgc gtgtcttct ccgaacatc aaaacaaca 
      tggagtatg acggttctt gttccgata gtttgcctg 
      ctagtgtta gggataatg aggttgatg acgaagatg 
      aagaaaatc gtcgacctc gtaatccct atccgagtt 
      accccagcg ctccttcac atccctatc cagacgctc 
      cacccccac agccatact ccactccct atccgccgg 
      caagcccat atcctaata atccctatc cctaccctc 
      agagttcat acggacaat atccctacc cgcaaaatc 
      cccaaccct atccaccct cataccctc agtacccgc 
      aatatccct cagaacctg acagaagtt cacgtgggt 
      ctagaggtt cagacccga ttacaaact ttttcaata 
      agctgtttc gttaagttc acagcttct ttgttcaat 
      gaagggagt ttcccttca aacagctag tcgcatttt 
      gtttctgca agggcgcca ggagccact ttgatgtaa 
      aactattct gggagtttc aattttgaa