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Gene information
General information
TC034626 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid24: 968315..1023869
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034626
    iBeetle screen iB_02731
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034626 RA
      MDSEPKNKV VVGVPAKTL SSQGYAICF FPEQPPRKI 
      VDVPAQENE EVFKKTEVK KVEAETIPL GNVKYIRLH 
      WRNRITYVI GFMAFTLSL NNAYHFPMN IYRYGGNFF 
      IPYTVVTIF VGYPLHVLQ TSMGQYSQR GVLWMWECA 
      PFCCGVGVA MVLACFLVA LYHNIFAAY ALVYFAHCF 
      HLVLPWTYC SEVEDADAH ACAPPSWYI SQNINLSSQ 
      EYFYNVVVD TNDKSHYLW SLGSIKWWL VLSLAFSWV 
      VCYIAVLRS VRSIGKICI FIIILSYFT LLTLIIVAL 
      QLKQFGKFS EVLSGVKGY VSWWNFEMW CVATIQVIV 
      ELGLCYGIP ITYASFCHF RNNYQTDSL ILCSMNYLH 
      SLIFFFIFF SFMVRYSCD TKDFLRRLP ENTIEMVYI 
      MYSEAFANL PGSQIWCMI FFLMLFFML IGTQIAFLE 
      AIMTAIYDN FVYLSKARP CINFLVCGG LALLSVVFC 
      FSNGLYQAV FVGSFPIWI TVLPLSILF VVGILFVYG 
      LNQYFRDMN LIQKDRNNL KLKFFLFAL LAVLIFALF 
      IVTIVRPAN TLSGDQNYD FTHVVIMWF VYAVFIGVI 
      IVFAFLWTM HCRVAQKQV FGSSVSWMR FYNERSEAR 
      GQFSYEYVP SYVWGWNSH
    CDS sequence
    • >TC034626 RA
      atggattcg gaaccaaaa aacaaagtt gttgttggt 
      gtaccagct aaaacttta tcttcgcaa ggatatgct 
      atttgtttt ttcccagag caacctcca aggaaaatt 
      gtcgatgtc cccgcccaa gaaaacgag gaagtgttt 
      aaaaaaact gaagtaaaa aaagttgaa gctgaaact 
      attccatta ggaaatgtc aagtacata agacttcac 
      tggaggaac cgaataacc tacgtgatc ggctttatg 
      gccttcact ctctcccta aacaacgcc taccatttc 
      cctatgaac atttatcgg tacggcggt aattttttt 
      atcccttac accgttgtc acgatattc gttggctac 
      cctctgcac gtgttgcaa acttccatg ggccaatac 
      agccaacgt ggggtgcta tggatgtgg gaatgcgcc 
      cctttttgc tgtggggtc ggagtggca atggttcta 
      gcctgcttc cttgtggct ttgtaccac aacattttc 
      gccgcttac gccctggtt tatttcgcc cattgcttc 
      cacttggtc ctcccatgg acctactgc agcgaggtg 
      gaggacgcc gatgcccac gcgtgcgcg cccccatca 
      tggtacatc tcccagaac atcaactta tcctcacag 
      gagtacttc tacaacgtt gtggtcgac accaacgac 
      aagtcgcat tacttgtgg tcgttgggc tcgattaag 
      tggtggctg gtcctgtcc ttggctttc tcatgggtt 
      gtttgctac attgccgtc cttcgcagt gttcgatcg 
      attgggaaa atttgcatt ttcattatt attttgtcg 
      tactttacg ctcctaacg ctgattatt gtggcgctt 
      cagttgaaa cagtttggg aaattttcg gaggttctt 
      tccggggtt aaggggtac gtgtcttgg tggaatttt 
      gagatgtgg tgtgtggcc acgattcag gtgattgtt 
      gagctgggc ttgtgttat ggcattccc ataacttac 
      gcttccttt tgccatttt cgaaacaac taccaaacc 
      gattcgctt attctgtgc tcgatgaac tatttgcat 
      tctttgatt tttttcttc atttttttc tcgtttatg 
      gttcggtat tcgtgcgat acgaaggat tttcttcgc 
      cgattgccc gagaacacg atcgaaatg gtttacatc 
      atgtattcg gaagctttt gctaattta cccgggagc 
      cagatttgg tgcatgatt ttttttctt atgctgttt 
      tttatgcta attgggaca caaattgcg tttcttgaa 
      gcgatcatg actgcaatc tatgataat ttcgtttac 
      ttgtccaaa gctcgcccg tgtatcaat tttctggtt 
      tgtggcggt ttggctctt ttgagtgtt gtgttctgt 
      ttcagcaac ggtttgtat caggctgtc ttcgtgggt 
      tcttttccc atttggatc actgtgctt ccgctttcg 
      attttattt gttgtaggg attttgttt gtttacggc 
      ttgaatcag tattttcgg gacatgaac ttgattcag 
      aaagataga aacaacttg aagttgaaa ttttttctg 
      tttgctttg ttggcagtt ttaattttt gctttgttt 
      attgtgaca attgtgagg ccggcgaat acgttgagt 
      ggtgatcag aattatgat ttcacacat gttgttata 
      atgtggttt gtttatgcg gtgtttatt ggtgtaatt 
      atcgtattt gcttttttg tggactatg cattgtaga 
      gttgcacag aagcaggtg ttcggttcg tcggtttct 
      tggatgagg ttttataat gagaggtct gaggctaga 
      ggacagttt agttacgag tatgtgcct tcgtatgtt 
      tgggggtgg aattcacat taa
    mRNA sequence
    • >TC034626 RA
      ctcaagtta tggattcgg aaccaaaaa acaaagttg 
      ttgttggtg taccagcta aaactttat cttcgcaag 
      gatatgcta tttgttttt tcccagagc aacctccaa 
      ggaaaattg tcgatgtcc ccgcccaag aaaacgagg 
      aagtgttta aaaaaactg aagtaaaaa aagttgaag 
      ctgaaacta ttccattag gaaatgtca agtacataa 
      gacttcact ggaggaacc gaataacct acgtgatcg 
      gctttatgg ccttcactc tctccctaa acaacgcct 
      accatttcc ctatgaaca tttatcggt acggcggta 
      attttttta tcccttaca ccgttgtca cgatattcg 
      ttggctacc ctctgcacg tgttgcaaa cttccatgg 
      gccaataca gccaacgtg gggtgctat ggatgtggg 
      aatgcgccc ctttttgct gtggggtcg gagtggcaa 
      tggttctag cctgcttcc ttgtggctt tgtaccaca 
      acattttcg ccgcttacg ccctggttt atttcgccc 
      attgcttcc acttggtcc tcccatgga cctactgca 
      gcgaggtgg aggacgccg atgcccacg cgtgcgcgc 
      ccccatcat ggtacatct cccagaaca tcaacttat 
      cctcacagg agtacttct acaacgttg tggtcgaca 
      ccaacgaca agtcgcatt acttgtggt cgttgggct 
      cgattaagt ggtggctgg tcctgtcct tggctttct 
      catgggttg tttgctaca ttgccgtcc ttcgcagtg 
      ttcgatcga ttgggaaaa tttgcattt tcattatta 
      ttttgtcgt actttacgc tcctaacgc tgattattg 
      tggcgcttc agttgaaac agtttggga aattttcgg 
      aggttcttt ccggggtta aggggtacg tgtcttggt 
      ggaattttg agatgtggt gtgtggcca cgattcagg 
      tgattgttg agctgggct tgtgttatg gcattccca 
      taacttacg cttcctttt gccattttc gaaacaact 
      accaaaccg attcgctta ttctgtgct cgatgaact 
      atttgcatt ctttgattt ttttcttca tttttttct 
      cgtttatgg ttcggtatt cgtgcgata cgaaggatt 
      ttcttcgcc gattgcccg agaacacga tcgaaatgg 
      tttacatca tgtattcgg aagcttttg ctaatttac 
      ccgggagcc agatttggt gcatgattt tttttctta 
      tgctgtttt ttatgctaa ttgggacac aaattgcgt 
      ttcttgaag cgatcatga ctgcaatct atgataatt 
      tcgtttact tgtccaaag ctcgcccgt gtatcaatt 
      ttctggttt gtggcggtt tggctcttt tgagtgttg 
      tgttctgtt tcagcaacg gtttgtatc aggctgtct 
      tcgtgggtt cttttccca tttggatca ctgtgcttc 
      cgctttcga ttttatttg ttgtaggga ttttgtttg 
      tttacggct tgaatcagt attttcggg acatgaact 
      tgattcaga aagatagaa acaacttga agttgaaat 
      tttttctgt ttgctttgt tggcagttt taatttttg 
      ctttgttta ttgtgacaa ttgtgaggc cggcgaata 
      cgttgagtg gtgatcaga attatgatt tcacacatg 
      ttgttataa tgtggtttg tttatgcgg tgtttattg 
      gtgtaatta tcgtatttg cttttttgt ggactatgc 
      attgtagag ttgcacaga agcaggtgt tcggttcgt 
      cggtttctt ggatgaggt tttataatg agaggtctg 
      aggctagag gacagttta gttacgagt atgtgcctt 
      cgtatgttt gggggtgga attcacatt aataaagca 
      acattttta ataactaat ttgttgttt taatataca 
      tcctaatcc cga