Status
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Gene information
General information
TC032586
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC010352,TC010348,TC010351,TC010350
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 24192177..24210781
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032586
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02730

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032586 RA
      MLVDKKVWE NSILQENTR QWELFRKIP FPFQIKVCF 
      FDVQNVDEI LQGAKPVVK EVGPYVYRL FRWKDDIEW 
      NDDEISYYD YMKFEFDKN ASGKLSDED VLTILNSPF 
      NALLMTVEE LSSTLLATV NDALPIIFN KNNGLFITA 
      KVKDLLFEG MIICENGGE SDFAAKMIC KQFKAKAKV 
      AKGMSIVNK SINYSYLSF KNNTHLGRL TIKSGLKEK 
      EDVSTLVKY NNESHVSVW RDDNPVCNE VKSTTTFRP 
      YVTPNMTFD VFSEDICRR LKMAYQSRE MVKGIQGYK 
      YVVTNDSFN APKVNTDNY CYCVNRSRT LEGDFGCLL 
      DGLLDMTNC VGKG
    • >TC032586 RB
      MCIAKYESH YNTAAINTQ TGDHGLFQI SQIYWCSNS 
      NKPGKGCNA KCSEFRDND IRDDVACVK KIYKEHQRL 
      SGNGFNAWV AYKKYCTGN NDHFIKGYK NLRVNISKS 
      KMNPLSKLL VLLFVSLCV SLQIEAKVF KRCELAKEL 
      KNKHHIPGN QLATWMCIA NYESGFNTA AINKKTGDH 
      GLFQISQIY WCSNSNKPG KACNAKCSD FRNNDIKDD 
      VACVKKIYN EHQKLSGNG FNAWVAYKK YCRGNNNNN 
      LSTVAKMKL LAVVAIFAF ASLSEAKIF DKCEFANTI 
      RGYGLFPAE HISTWVCIA NYESAFNTD ATNTVTGDH 
      GIYQISQIY WCSTGDSPG GGCNKRCAD FHNDDISDD 
      SVCAKAIFD EHQRLSGNG FNAWTTYAP YCSGDNSGW 
      IAGC
    CDS sequence
    • >TC032586 RA
      atgcttgta gataaaaaa gtttgggag aattccatt 
      ttacaagaa aatactcgt cagtgggaa ctatttaga 
      aaaattcca tttccattt caaattaaa gtctgcttt 
      tttgatgtc cagaacgta gatgagata ctgcaaggt 
      gcaaaacct gttgttaaa gaagttgga ccttatgtc 
      tacagatta tttcgctgg aaagacgat attgaatgg 
      aatgatgat gaaatatcg tattatgat tacatgaag 
      tttgaattt gataaaaat gcttctgga aaattatca 
      gatgaagat gttctaaca attcttaat tcccctttt 
      aacgctctg ctgatgact gtcgaagaa ttatcttcc 
      accctcctg gccactgtc aacgatgct ctccctatc 
      atttttaac aaaaacaac ggcctcttc ataacagca 
      aaagtgaaa gatttgctg ttcgaagga atgatcatt 
      tgcgaaaat gggggcgaa agcgacttt gcagctaag 
      atgatttgt aagcagttc aaagcgaaa gctaaagtc 
      gccaaaggc atgtccatc gtcaacaaa tcaatcaat 
      tattcgtac ctcagtttt aaaaataac actcatctt 
      ggacgttta acaatcaaa agtggctta aaggaaaaa 
      gaagacgtt tcaactttg gtaaaatat aacaatgaa 
      agtcacgtc tccgtctgg agggatgat aatccggtg 
      tgtaatgaa gtcaaaagc acgacgact tttcggcct 
      tatgttacg ccaaatatg acgtttgat gtgttttcc 
      gaggacatc tgcagacgt ttaaaaatg gcatatcag 
      tccagagaa atggtgaaa gggatacaa ggatataaa 
      tatgttgta acgaatgat tccttcaat gctcctaaa 
      gtaaacaca gacaattat tgttactgt gttaaccgg 
      agtagaact ttggaagga gattttgga tgtttacta 
      gatggactt ttggatatg acaaattgt gtaggtaag 
      ggataa
    • >TC032586 RB
      atgtgcatc gcaaaatat gaatcgcat tacaacact 
      gctgctatc aacacgcaa acaggagac catggcttg 
      tttcaaata agccaaatt tattggtgt tcaaacagc 
      aacaaacca gggaaaggg tgcaatgcc aaatgttcc 
      gaatttcga gataacgac attcgggac gatgttgcc 
      tgtgtcaag aagatctac aaagaacat caaagactg 
      tccggaaat gggttcaat gcctgggtg gcttacaag 
      aaatattgc acaggaaat aatgatcat ttcatcaaa 
      ggatacaaa aacttgcgt gttaacatc agtaagtcc 
      aagatgaat cctttgagc aaattactt gtgctcctc 
      ttcgtttcg ctttgtgtt tcacttcaa atagaagca 
      aaagtcttc aaacgatgt gaacttgcc aaagaattg 
      aaaaacaag caccacatc cctggaaat caactggca 
      acatggatg tgcatcgca aattacgaa tcgggtttc 
      aacactgct gctatcaac aagaaaaca ggagaccat 
      ggcttgttt caaataagc caaatctac tggtgttca 
      aacagcaac aaaccggga aaagcatgc aatgcgaaa 
      tgttccgat tttcgaaat aacgacatt aaagacgat 
      gttgcctgt gtcaagaag atctacaac gaacatcaa 
      aaactttct ggaaatggg ttcaatgct tgggtggct 
      tacaagaaa tactgcaga ggaaataat aacaataac 
      ttgtctaca gttgcaaaa atgaaactt ctcgctgtg 
      gttgctatt ttcgccttt gcttcgctg agcgaagca 
      aaaattttc gataagtgt gagtttgcc aacactatc 
      agaggctat ggtctgttc cctgcagag catatttcc 
      acatgggtc tgtatcgca aattacgaa tccgctttt 
      aacaccgat gccaccaat actgtgact ggagaccat 
      gggatttat caaataagt caaatttac tggtgttcg 
      actggagat agtccagga ggtggttgc aataagaga 
      tgtgccgat ttccataat gatgatatt agtgacgat 
      tccgtgtgt gctaaagcc atcttcgat gaacatcag 
      agactttcc ggaaatggg ttcaatgcc tggacaact 
      tatgcgcct tattgtagt ggagataat tctgggtgg 
      attgctgga tgttag
    mRNA sequence
    • >TC032586 RA
      ccaacggta cagttccat ttgcatttc atgcaagaa 
      aatcaaaaa attgcaaca aatctaata taaatataa 
      caatcaaat caccaacat gaaaataat agtgaatac 
      aaaagtttt aatttattg tgcagtgtc tggaactgt 
      gctttcggt aatgggagt acttctcgg ttttaaaat 
      ttttccaat gcttgtaga taaaaaagt ttgggagaa 
      ttccatttt acaagaaaa tactcgtca gtgggaact 
      atttagaaa aattccatt tccatttca aattaaagt 
      ctgcttttt tgatgtcca gaacgtaga tgagatact 
      gcaaggtgc aaaacctgt tgttaaaga agttggacc 
      ttatgtcta cagattatt tcgctggaa agacgatat 
      tgaatggaa tgatgatga aatatcgta ttatgatta 
      catgaagtt tgaatttga taaaaatgc ttctggaaa 
      attatcaga tgaagatgt tctaacaat tcttaattc 
      cccttttaa cgctctgct gatgactgt cgaagaatt 
      atcttccac cctcctggc cactgtcaa cgatgctct 
      ccctatcat ttttaacaa aaacaacgg cctcttcat 
      aacagcaaa agtgaaaga tttgctgtt cgaaggaat 
      gatcatttg cgaaaatgg gggcgaaag cgactttgc 
      agctaagat gatttgtaa gcagttcaa agcgaaagc 
      taaagtcgc caaaggcat gtccatcgt caacaaatc 
      aatcaatta ttcgtacct cagttttaa aaataacac 
      tcatcttgg acgtttaac aatcaaaag tggcttaaa 
      ggaaaaaga agacgtttc aactttggt aaaatataa 
      caatgaaag tcacgtctc cgtctggag ggatgataa 
      tccggtgtg taatgaagt caaaagcac gacgacttt 
      tcggcctta tgttacgcc aaatatgac gtttgatgt 
      gttttccga ggacatctg cagacgttt aaaaatggc 
      atatcagtc cagagaaat ggtgaaagg gatacaagg 
      atataaata tgttgtaac gaatgattc cttcaatgc 
      tcctaaagt aaacacaga caattattg ttactgtgt 
      taaccggag tagaacttt ggaaggaga ttttggatg 
      tttactaga tggactttt ggatatgac aaattgtgt 
      aggtaaggg ataaatttt attggaatt tccttaaac 
      attgagaac actgataaa caaagacag taattattg 
      ttttgttta catttaata aagataaga aaggttt
    • >TC032586 RB
      cagtatatc caagatgag tcctttgag caaaatact 
      tttaaagca aaaattttc gaacgatgt gaacttgct 
      aaagaattg aaaaagaac catttgcct ggaactcaa 
      ctggcaaca tggatgtgc atcgcaaaa tatgaatcg 
      cattacaac actgctgct atcaacacg caaacagga 
      gaccatggc ttgtttcaa ataagccaa atttattgg 
      tgttcaaac agcaacaaa ccagggaaa gggtgcaat 
      gccaaatgt tccgaattt cgagataac gacattcgg 
      gacgatgtt gcctgtgtc aagaagatc tacaaagaa 
      catcaaaga ctgtccgga aatgggttc aatgcctgg 
      gtggcttac aagaaatat tgcacagga aataatgat 
      catttcatc aaaggatac aaaaacttg cgtgttaac 
      atcagtaag tccaagatg aatcctttg agcaaatta 
      cttgtgctc ctcttcgtt tcgctttgt gtttcactt 
      caaatagaa gcaaaagtc ttcaaacga tgtgaactt 
      gccaaagaa ttgaaaaac aagcaccac atccctgga 
      aatcaactg gcaacatgg atgtgcatc gcaaattac 
      gaatcgggt ttcaacact gctgctatc aacaagaaa 
      acaggagac catggcttg tttcaaata agccaaatc 
      tactggtgt tcaaacagc aacaaaccg ggaaaagca 
      tgcaatgcg aaatgttcc gattttcga aataacgac 
      attaaagac gatgttgcc tgtgtcaag aagatctac 
      aacgaacat caaaaactt tctggaaat gggttcaat 
      gcttgggtg gcttacaag aaatactgc agaggaaat 
      aataacaat aacttgtct acagttgca aaaatgaaa 
      cttctcgct gtggttgct attttcgcc tttgcttcg 
      ctgagcgaa gcaaaaatt ttcgataag tgtgagttt 
      gccaacact atcagaggc tatggtctg ttccctgca 
      gagcatatt tccacatgg gtctgtatc gcaaattac 
      gaatccgct tttaacacc gatgccacc aatactgtg 
      actggagac catgggatt tatcaaata agtcaaatt 
      tactggtgt tcgactgga gatagtcca ggaggtggt 
      tgcaataag agatgtgcc gatttccat aatgatgat 
      attagtgac gattccgtg tgtgctaaa gccatcttc 
      gatgaacat cagagactt tccggaaat gggttcaat 
      gcctggaca acttatgcg ccttattgt agtggagat 
      aattctggg tggattgct ggatgttag atttagctt 
      ttttacatg ttaataaaa aaattcaaa gaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY