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Gene information
General information
TC003821 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 21718839..21728015
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003821
    iBeetle screen iB_02728

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality early (L5/6) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003821 RA
      MACQFSKDG KYFAQITSE GKLKVWNTL SNTFEQEFT 
      PDFHLTSPC TCLHFISSE AASKQSGSP RKKKRKESL 
      GESNTCVAL GTSNGRLLL YSLVKGDLE YIVDSATSQ 
      SVNCLSSIT NEFVYSGAD THVIVWNLS KRNVSSKWN 
      AGNDKIYAI LLTPLGDKL LTASKSIKL WDVKSKEVL 
      KTFTGHSSP VTMLEYINP RSDAADAYV VSGSKGDRL 
      LSCWNLNDS VDGKNAVAS FLMEDIVNN LSIFVDHDG 
      SSRMAATVR SGVVHIYHH TLNGKKSNK PIKPKTTIQ 
      IVSDSGTKD AVNPIYITA AAYRSAETL CICYGSAVV 
      QTFEDITIS SYKKVQCLL REDIHKKTV SKENQVTKV 
      RTPVVGNDV HYLTTQTSS APSKRKNDG QQEVPMEKR 
      LENLMVNEE DSSSKVPKA NNAAQLLIQ GLHSKDKNI 
      LRTVLFKKD EQLIKNTIK RIPLSLITP LFEELTKLI 
      QGKTLSRQV GLTWLKNLI LIHSGLLLS NPDLSSFFG 
      EALGSIEAG LALQSPRNK LMGRLELLI AQINHTPQQ 
      DSHDNEALL VFNDKASSD SENEGMEFE AHSSSENEW 
      EEEDSEGGD NENEKVDES DYENDDGSD EPMSS
    • >TC003821 RB
      MACQFSKDG KYFAQITSE GKLKVWNTL SNTFEQEFT 
      PDFHLTSPC TCLHFISSE AASKSGSPR KKKRKESLG 
      ESNTCVALG TSNGRLLLY SLVKGDLEY IVDSATSQS 
      VNCLSSITN EFVYSGADT HVIVWNLSK RNVSSKWNA 
      GNDKIYAIL LTPLGDKLL TASKSIKLW DVKSKEVLK 
      TFTGHSSPV TMLEYINPR SDAADAYVV SGSKGDRLL 
      SCWNLNDSV DGKNAVASF LMEDIVNNL SIFVDHDGS 
      SRMAATVRS GVVHIYHHT LNGKKSNKP IKPKTTIQI 
      VSDSGTKDA VNPIYITAA AYRSAETLC ICYGSAVVQ 
      TFEDITISS YKKVQCLLR EDIHKKTVS KENQVTKVR 
      TPVVGNDVH YLTTQTSSA PSKRKNDGQ QEVPMEKRL 
      ENLMVNEED SSSKVPKAN NAAQLLIQG LHSKDKNIL 
      RTVLFKKDE QLIKNTIKR IPLSLITPL FEELTKLIQ 
      GKTLSRQVG LTWLKNLIL IHSGLLLSN PDLSSFFGE 
      ALGSIEAGL ALQSPRNKL MGRLELLIA QINHTPQQD 
      SHDNEALLV FNDKASSDS ENEGMEFEA HSSSENEWE 
      EEDSEGGDN ENEKVDESD YENDDGSDE PMSS
    CDS sequence
    • >TC003821 RA
      atggcatgc caattcagt aaagacggc aaatatttt 
      gctcaaatc acatcagag gggaagcta aaagtgtgg 
      aacaccctc tcaaatacc tttgaacaa gaatttaca 
      ccagatttc cacttaaca tcgccctgt acttgttta 
      catttcata agttcagaa gccgcaagc aagcagtcg 
      gggtcccct cgcaagaaa aagaggaaa gaatcactt 
      ggggagtca aacacttgt gttgcttta gggactagc 
      aatggacgt cttttactt tattcctta gtaaagggg 
      gacttagaa tacatagtt gatagtgct acgagtcag 
      tcggttaat tgtttatct tcaattaca aacgagttt 
      gtatattct ggtgctgac actcatgta atagtgtgg 
      aatttaagt aaacgaaac gttagcagt aagtggaat 
      gctggtaac gacaaaata tacgcgatt ttactcaca 
      ccactcggt gacaagcta ttaactgcc tcgaaaagt 
      atcaaactc tgggatgtg aagtcaaaa gaagtgttg 
      aaaacattt actgggcat tcttcacct gtgacaatg 
      cttgaatac atcaatccg agatctgat gcagcagat 
      gcgtatgtt gttagtggg tcaaaaggg gatcgattg 
      ttaagttgt tggaattta aatgatagt gtggatggg 
      aaaaatgca gttgctagt tttttaatg gaggatatt 
      gtaaataat ttgtcaatt tttgttgat catgatggc 
      tcatctcgc atggctgca acggtgagg agtggggtt 
      gtgcatatt taccaccat acattaaat ggaaaaaaa 
      tctaacaaa cccataaaa cctaaaaca acgatccag 
      attgtgtcc gattccggg accaaggat gccgtcaat 
      ccaatatac ataacagca gcagcatac agaagcgct 
      gaaactctg tgtatatgt tatggcagt gcagttgtt 
      caaactttt gaggacatt acaatttcg agttataag 
      aaagttcag tgcctttta agggaagac attcacaaa 
      aagacagtt tctaaagaa aatcaagtt acgaaagtt 
      cgtacacct gttgtggga aatgatgta cattactta 
      acaactcaa acgagtagt gctcccagc aagaggaaa 
      aatgacggc caacaggag gtccctatg gaaaaaaga 
      ttggagaat ttgatggtg aatgaagaa gacagcagc 
      tcgaaagta cccaaagcc aacaatgcg gcacaatta 
      ttaatacaa gggttgcac agtaaagat aaaaatatt 
      ttacgaacg gttttattt aaaaaggac gaacaactc 
      atcaaaaac acgatcaaa aggatacca ttgtcccta 
      ataacaccg ctgtttgag gaacttact aaacttata 
      caggggaaa acgctgtcg cgtcaggtg ggcttaacg 
      tggctgaaa aatttaatt ttgattcat tctggactt 
      ctgttgtcg aatcccgat ctgtcgagt ttttttggg 
      gaagcttta gggagcatt gaagccgga ttagcacta 
      cagagtcca agaaataaa ttaatgggg cgcttggag 
      ttgttaatc gcgcagata aatcataca ccacaacag 
      gattcacac gataatgaa gcattgctc gttttcaac 
      gataaagct tcgagtgat tcggagaat gaaggtatg 
      gagtttgag gctcattcg agtagtgaa aacgagtgg 
      gaagaggaa gatagtgaa ggtggggat aatgaaaac 
      gagaaggtg gatgagagt gattatgaa aatgatgat 
      gggtcagat gagcccatg tcttcataa
    • >TC003821 RB
      atggcatgc caattcagt aaagacggc aaatatttt 
      gctcaaatc acatcagag gggaagcta aaagtgtgg 
      aacaccctc tcaaatacc tttgaacaa gaatttaca 
      ccagatttc cacttaaca tcgccctgt acttgttta 
      catttcata agttcagaa gccgcaagc aagtcgggg 
      tcccctcgc aagaaaaag aggaaagaa tcacttggg 
      gagtcaaac acttgtgtt gctttaggg actagcaat 
      ggacgtctt ttactttat tccttagta aagggggac 
      ttagaatac atagttgat agtgctacg agtcagtcg 
      gttaattgt ttatcttca attacaaac gagtttgta 
      tattctggt gctgacact catgtaata gtgtggaat 
      ttaagtaaa cgaaacgtt agcagtaag tggaatgct 
      ggtaacgac aaaatatac gcgatttta ctcacacca 
      ctcggtgac aagctatta actgcctcg aaaagtatc 
      aaactctgg gatgtgaag tcaaaagaa gtgttgaaa 
      acatttact gggcattct tcacctgtg acaatgctt 
      gaatacatc aatccgaga tctgatgca gcagatgcg 
      tatgttgtt agtgggtca aaaggggat cgattgtta 
      agttgttgg aatttaaat gatagtgtg gatgggaaa 
      aatgcagtt gctagtttt ttaatggag gatattgta 
      aataatttg tcaattttt gttgatcat gatggctca 
      tctcgcatg gctgcaacg gtgaggagt ggggttgtg 
      catatttac caccataca ttaaatgga aaaaaatct 
      aacaaaccc ataaaacct aaaacaacg atccagatt 
      gtgtccgat tccgggacc aaggatgcc gtcaatcca 
      atatacata acagcagca gcatacaga agcgctgaa 
      actctgtgt atatgttat ggcagtgca gttgttcaa 
      acttttgag gacattaca atttcgagt tataagaaa 
      gttcagtgc cttttaagg gaagacatt cacaaaaag 
      acagtttct aaagaaaat caagttacg aaagttcgt 
      acacctgtt gtgggaaat gatgtacat tacttaaca 
      actcaaacg agtagtgct cccagcaag aggaaaaat 
      gacggccaa caggaggtc cctatggaa aaaagattg 
      gagaatttg atggtgaat gaagaagac agcagctcg 
      aaagtaccc aaagccaac aatgcggca caattatta 
      atacaaggg ttgcacagt aaagataaa aatatttta 
      cgaacggtt ttatttaaa aaggacgaa caactcatc 
      aaaaacacg atcaaaagg ataccattg tccctaata 
      acaccgctg tttgaggaa cttactaaa cttatacag 
      gggaaaacg ctgtcgcgt caggtgggc ttaacgtgg 
      ctgaaaaat ttaattttg attcattct ggacttctg 
      ttgtcgaat cccgatctg tcgagtttt tttggggaa 
      gctttaggg agcattgaa gccggatta gcactacag 
      agtccaaga aataaatta atggggcgc ttggagttg 
      ttaatcgcg cagataaat catacacca caacaggat 
      tcacacgat aatgaagca ttgctcgtt ttcaacgat 
      aaagcttcg agtgattcg gagaatgaa ggtatggag 
      tttgaggct cattcgagt agtgaaaac gagtgggaa 
      gaggaagat agtgaaggt ggggataat gaaaacgag 
      aaggtggat gagagtgat tatgaaaat gatgatggg 
      tcagatgag cccatgtct tcataa
    mRNA sequence
    • >TC003821 RA
      cggtaagtc agaaagtgg gaaaaagtt aggttacga 
      aaacaagtt aatggcggt tagtgcaag aatggcatg 
      ccaattcag taaagacgg caaatattt tgctcaaat 
      cacatcaga ggggaagct aaaagtgtg gaacaccct 
      ctcaaatac ctttgaaca agaatttac accagattt 
      ccacttaac atcgccctg tacttgttt acatttcat 
      aagttcaga agccgcaag caagcagtc ggggtcccc 
      tcgcaagaa aaagaggaa agaatcact tggggagtc 
      aaacacttg tgttgcttt agggactag caatggacg 
      tcttttact ttattcctt agtaaaggg ggacttaga 
      atacatagt tgatagtgc tacgagtca gtcggttaa 
      ttgtttatc ttcaattac aaacgagtt tgtatattc 
      tggtgctga cactcatgt aatagtgtg gaatttaag 
      taaacgaaa cgttagcag taagtggaa tgctggtaa 
      cgacaaaat atacgcgat tttactcac accactcgg 
      tgacaagct attaactgc ctcgaaaag tatcaaact 
      ctgggatgt gaagtcaaa agaagtgtt gaaaacatt 
      tactgggca ttcttcacc tgtgacaat gcttgaata 
      catcaatcc gagatctga tgcagcaga tgcgtatgt 
      tgttagtgg gtcaaaagg ggatcgatt gttaagttg 
      ttggaattt aaatgatag tgtggatgg gaaaaatgc 
      agttgctag ttttttaat ggaggatat tgtaaataa 
      tttgtcaat ttttgttga tcatgatgg ctcatctcg 
      catggctgc aacggtgag gagtggggt tgtgcatat 
      ttaccacca tacattaaa tggaaaaaa atctaacaa 
      acccataaa acctaaaac aacgatcca gattgtgtc 
      cgattccgg gaccaagga tgccgtcaa tccaatata 
      cataacagc agcagcata cagaagcgc tgaaactct 
      gtgtatatg ttatggcag tgcagttgt tcaaacttt 
      tgaggacat tacaatttc gagttataa gaaagttca 
      gtgcctttt aagggaaga cattcacaa aaagacagt 
      ttctaaaga aaatcaagt tacgaaagt tcgtacacc 
      tgttgtggg aaatgatgt acattactt aacaactca 
      aacgagtag tgctcccag caagaggaa aaatgacgg 
      ccaacagga ggtccctat ggaaaaaag attggagaa 
      tttgatggt gaatgaaga agacagcag ctcgaaagt 
      acccaaagc caacaatgc ggcacaatt attaataca 
      agggttgca cagtaaaga taaaaatat tttacgaac 
      ggttttatt taaaaagga cgaacaact catcaaaaa 
      cacgatcaa aaggatacc attgtccct aataacacc 
      gctgtttga ggaacttac taaacttat acaggggaa 
      aacgctgtc gcgtcaggt gggcttaac gtggctgaa 
      aaatttaat tttgattca ttctggact tctgttgtc 
      gaatcccga tctgtcgag tttttttgg ggaagcttt 
      agggagcat tgaagccgg attagcact acagagtcc 
      aagaaataa attaatggg gcgcttgga gttgttaat 
      cgcgcagat aaatcatac accacaaca ggattcaca 
      cgataatga agcattgct cgttttcaa cgataaagc 
      ttcgagtga ttcggagaa tgaaggtat ggagtttga 
      ggctcattc gagtagtga aaacgagtg ggaagagga 
      agatagtga aggtgggga taatgaaaa cgagaaggt 
      ggatgagag tgattatga aaatgatga tgggtcaga 
      tgagcccat gtcttcata atttttgtt gttgatttt 
      tttcaacta ataaaaaca ttttggctc ctgattttg 
      taaattatt tacgaaaat aacatttta ttacagctg 
      tactatgga ttttaagta tagtgacaa gcgatattg 
      taatttttt aataaatgt taaacaaaa tt
    • >TC003821 RB
      cggtaagtc agaaagtgg gaaaaagtt aggttacga 
      aaacaagtt aatggcggt tagtgcaag aatggcatg 
      ccaattcag taaagacgg caaatattt tgctcaaat 
      cacatcaga ggggaagct aaaagtgtg gaacaccct 
      ctcaaatac ctttgaaca agaatttac accagattt 
      ccacttaac atcgccctg tacttgttt acatttcat 
      aagttcaga agccgcaag caagtcggg gtcccctcg 
      caagaaaaa gaggaaaga atcacttgg ggagtcaaa 
      cacttgtgt tgctttagg gactagcaa tggacgtct 
      tttacttta ttccttagt aaaggggga cttagaata 
      catagttga tagtgctac gagtcagtc ggttaattg 
      tttatcttc aattacaaa cgagtttgt atattctgg 
      tgctgacac tcatgtaat agtgtggaa tttaagtaa 
      acgaaacgt tagcagtaa gtggaatgc tggtaacga 
      caaaatata cgcgatttt actcacacc actcggtga 
      caagctatt aactgcctc gaaaagtat caaactctg 
      ggatgtgaa gtcaaaaga agtgttgaa aacatttac 
      tgggcattc ttcacctgt gacaatgct tgaatacat 
      caatccgag atctgatgc agcagatgc gtatgttgt 
      tagtgggtc aaaagggga tcgattgtt aagttgttg 
      gaatttaaa tgatagtgt ggatgggaa aaatgcagt 
      tgctagttt tttaatgga ggatattgt aaataattt 
      gtcaatttt tgttgatca tgatggctc atctcgcat 
      ggctgcaac ggtgaggag tggggttgt gcatattta 
      ccaccatac attaaatgg aaaaaaatc taacaaacc 
      cataaaacc taaaacaac gatccagat tgtgtccga 
      ttccgggac caaggatgc cgtcaatcc aatatacat 
      aacagcagc agcatacag aagcgctga aactctgtg 
      tatatgtta tggcagtgc agttgttca aacttttga 
      ggacattac aatttcgag ttataagaa agttcagtg 
      ccttttaag ggaagacat tcacaaaaa gacagtttc 
      taaagaaaa tcaagttac gaaagttcg tacacctgt 
      tgtgggaaa tgatgtaca ttacttaac aactcaaac 
      gagtagtgc tcccagcaa gaggaaaaa tgacggcca 
      acaggaggt ccctatgga aaaaagatt ggagaattt 
      gatggtgaa tgaagaaga cagcagctc gaaagtacc 
      caaagccaa caatgcggc acaattatt aatacaagg 
      gttgcacag taaagataa aaatatttt acgaacggt 
      tttatttaa aaaggacga acaactcat caaaaacac 
      gatcaaaag gataccatt gtccctaat aacaccgct 
      gtttgagga acttactaa acttataca ggggaaaac 
      gctgtcgcg tcaggtggg cttaacgtg gctgaaaaa 
      tttaatttt gattcattc tggacttct gttgtcgaa 
      tcccgatct gtcgagttt ttttgggga agctttagg 
      gagcattga agccggatt agcactaca gagtccaag 
      aaataaatt aatggggcg cttggagtt gttaatcgc 
      gcagataaa tcatacacc acaacagga ttcacacga 
      taatgaagc attgctcgt tttcaacga taaagcttc 
      gagtgattc ggagaatga aggtatgga gtttgaggc 
      tcattcgag tagtgaaaa cgagtggga agaggaaga 
      tagtgaagg tggggataa tgaaaacga gaaggtgga 
      tgagagtga ttatgaaaa tgatgatgg gtcagatga 
      gcccatgtc ttcataatt tttgttgtt gattttttt 
      caactaata aaaacattt tggctcctg attttgtaa 
      attatttac gaaaataac attttatta cagctgtac 
      tatggattt taagtatag tgacaagcg atattgtaa 
      ttttttaat aaatgttaa acaaaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY