Status
Please wait ...
Gene information
General information
TC003819 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 21552531..21558248
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003819
    iBeetle screen iB_02725

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003819 RA
      MNIPTCLVG SFCLLTLAE CMTNMTLKR TVEDDYYMN 
      YDYEISNDS FNSELKVDS FLLPNPENI DSKYAVDVL 
      PNVELPDDS LSDTDLKDH DFIDNLMYV YYGSHNKNG 
      HSYGPEVII VGSVLSCAA QLLTIFFVL LKKNLNGKK 
      EMKNLFLHM MGSFCFSNL LFMLGVYAI KNSVKCLMV 
      AFLLHYLHL LTAFWMFLY CYYIYKRFS SSGVPKNKY 
      FFSVGYGVP LVFSLLSYV VSPYSFETK RFCFMSVQK 
      GMIINYMLP ISLLIVITT IYSLNGIRK INIELSKLE 
      FSSSVESLN ALKNELDVV MNDKKQECA DEEVVSLRE 
      SKTCLKLMC VVQTGYDIV WFIVVLALE SNSENNSMS 
      IVYSVTSCL LNWYIFIKA KSFLPNIMH VSRSIDNVL 
      LHQEQQATN AHKENSSLS RRGSSDSVP LLVNSENCT 
      EMRELRLEY ISTISN
    CDS sequence
    • >TC003819 RA
      atgaacatt ccgacatgt cttgtgggt agtttttgt 
      ttgttaact cttgcagaa tgcatgaca aatatgact 
      ttaaagcgc accgtcgaa gacgattat tacatgaat 
      tatgactac gaaatttcg aacgactcg ttcaattcc 
      gagctgaaa gttgattcc tttctgttg ccaaatccc 
      gaaaatatt gactcaaaa tatgcagtt gacgtgctc 
      cccaatgtt gaactcccc gatgattcg ctctcagac 
      accgacttg aaagaccac gatttcatc gataattta 
      atgtacgtt tattacggc tctcataat aagaacgga 
      cacagttat gggcctgag gtgataatt gttggctca 
      gtgctcagc tgcgctgct cagctactg acgattttt 
      ttcgttcta ctcaagaag aatttgaat gggaaaaag 
      gagatgaaa aatttgttc ttgcacatg atgggctcg 
      ttctgtttc tccaatttg ctcttcatg ttaggtgtc 
      tatgcgata aaaaattcg gttaagtgt ttgatggta 
      gccttcctc ttgcactac ttgcattta cttacagcg 
      ttttggatg ttcttgtat tgttattat atctacaag 
      cgattctct agtagtggt gtcccgaaa aataaatat 
      tttttctcg gtgggttat ggggttcca ctcgttttt 
      agtcttctt tcatatgtg gtgtcgccg tacagtttc 
      gaaacgaag cgattttgc ttcatgtca gtacaaaaa 
      ggaatgata ataaactat atgcttcca atatctcta 
      ctaatcgtt ataacaacg atctactcg ctcaatggt 
      ataagaaaa ataaatata gaattgtcc aaattagag 
      tttagtagt tcagtcgag tcattaaac gctttaaag 
      aacgaactg gacgttgta atgaacgac aagaaacag 
      gaatgtgca gacgaagaa gtcgtcagt ttaagggaa 
      tcgaagact tgcttgaaa ctgatgtgt gtggtgcaa 
      actggatac gatatagtg tggttcatt gtagttctg 
      gctcttgag agcaacagt gaaaataac agtatgtcg 
      attgtatat tcggttact tcgtgttta ctgaactgg 
      tacatcttc attaaagct aaatctttt cttccaaat 
      attatgcac gtttctcgt tcaattgac aatgttctt 
      ctacaccag gagcagcaa gcgacaaat gcacataag 
      gagaattcg tcgttgtcg aggagaggg tcttccgac 
      agtgttcct cttttggta aattcggaa aattgtacc 
      gaaatgaga gaactgcgc cttgaatac attagtact 
      ataagcaac taa
    mRNA sequence
    • >TC003819 RA
      caatggatg aactcggga tatttgctc aatttccct 
      cccgaattt tgttatgaa cattccgac atgtcttgt 
      gggtagttt ttgtttgtt aactcttgc agaatgcat 
      gacaaatat gactttaaa gcgcaccgt cgaagacga 
      ttattacat gaattatga ctacgaaat ttcgaacga 
      ctcgttcaa ttccgagct gaaagttga ttcctttct 
      gttgccaaa tcccgaaaa tattgactc aaaatatgc 
      agttgacgt gctccccaa tgttgaact ccccgatga 
      ttcgctctc agacaccga cttgaaaga ccacgattt 
      catcgataa tttaatgta cgtttatta cggctctca 
      taataagaa cggacacag ttatgggcc tgaggtgat 
      aattgttgg ctcagtgct cagctgcgc tgctcagct 
      actgacgat ttttttcgt tctactcaa gaagaattt 
      gaatgggaa aaaggagat gaaaaattt gttcttgca 
      catgatggg ctcgttctg tttctccaa tttgctctt 
      catgttagg tgtctatgc gataaaaaa ttcggttaa 
      gtgtttgat ggtagcctt cctcttgca ctacttgca 
      tttacttac agcgttttg gatgttctt gtattgtta 
      ttatatcta caagcgatt ctctagtag tggtgtccc 
      gaaaaataa atatttttt ctcggtggg ttatggggt 
      tccactcgt ttttagtct tctttcata tgtggtgtc 
      gccgtacag tttcgaaac gaagcgatt ttgcttcat 
      gtcagtaca aaaaggaat gataataaa ctatatgct 
      tccaatatc tctactaat cgttataac aacgatcta 
      ctcgctcaa tggtataag aaaaataaa tatagaatt 
      gtccaaatt agagtttag tagttcagt cgagtcatt 
      aaacgcttt aaagaacga actggacgt tgtaatgaa 
      cgacaagaa acaggaatg tgcagacga agaagtcgt 
      cagtttaag ggaatcgaa gacttgctt gaaactgat 
      gtgtgtggt gcaaactgg atacgatat agtgtggtt 
      cattgtagt tctggctct tgagagcaa cagtgaaaa 
      taacagtat gtcgattgt atattcggt tacttcgtg 
      tttactgaa ctggtacat cttcattaa agctaaatc 
      ttttcttcc aaatattat gcacgtttc tcgttcaat 
      tgacaatgt tcttctaca ccaggagca gcaagcgac 
      aaatgcaca taaggagaa ttcgtcgtt gtcgaggag 
      agggtcttc cgacagtgt tcctctttt ggtaaattc 
      ggaaaattg taccgaaat gagagaact gcgccttga 
      atacattag tactataag caactaaag cgctgcaat 
      attattcga acgtagaca ttgtatact cactagtca 
      gaataatgt ttatttttt cttttcaga gtaaattgt 
      tgaaaagtt actctttgg accagtttg tcgtttttg 
      tggtctgag ggatggttc tttattcta acgtaattt 
      taagacatc ggcctatat cagtattag aagttctta 
      cttcttacc tctatttct actgtaaac aatgttcta 
      aaaaagcac aagttgtcc gtgtaagca accaaaaat 
      aaatgttgt ttatctaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY