General information | |||
TC032539
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 17888353..17890124 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC032539 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02722 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC032539 RA
MYFVTPGLI FTVIALWLL WSFLWKNFF ATKIKGPWA FPLIGSAYV LFGGTHNIL ENTVRVVSK YRPLCKLWL GNKLTIFPS NPDDIQMIL NRSLEKEEG YRYIKTVFG HGLFTSPVS EWKGHRKVI VSTLNQQVL NSFMDTYNI YTRELIEKL KIDTKDGQH IDMFPIMTQ CTLDIICSS IMGTQMNAM KEESQFIMW MTRVAELAI IRMFNIYLW PELFWKLSP ISKESHELD QKIYNYVKT VIHNKRHKG LKSHTGKKI FLDYLVELT DREGRWTDE ELTNETRSV IMAGSDATA LTLSYCLVM LAMFQDIQD KVYEELCSV FGDSDRFVT VDDLPHLQY LDRFIKETL RVFPVTSMI GRELTTDMT IDGHFIPKG TSIGFPILY IHRNPEYYP DPLKFDPDR FLPEEVAKR HPCTFIPFS FGPRNCIGY RYAMMTMKV ILATLLRSF KMVHTPYKE ISELKIKFD IATKVDEGY PVRMELRKN VAAGTK
- >TC032539 RA
atgtatttc gtaactccc ggtctcatt tttacggta atagcactt tggttgttg tggtcattc ttgtggaaa aattttttc gctacgaag atcaaagga ccatgggcc tttccgctc atcggaagc gcctacgtc ctgtttggc ggaacacac aatattttg gaaaatacg gtccgagtg gtcagcaag taccggccg ttgtgtaaa ctgtggttg ggcaataag ttgacgatt ttcccctcg aaccctgac gacatccag atgatcctc aacaggtct ttggagaag gaggagggt tataggtac atcaagact gtttttgga cacggcttg ttcactagt cccgtaagc gagtggaag gggcatcgg aaagtcatc gtttcgact ctcaaccag caagttttg aacagtttt atggacacg tacaatatt tacacgcgg gaattgatc gagaaactc aaaattgac accaaggac gggcagcat atcgatatg ttccccatt atgacccag tgcaccttg gatatcatc tgcagctcc atcatgggg acgcagatg aatgccatg aaggaggaa agtcagttt attatgtgg atgacaaga gtggccgag cttgcaatc attcgcatg ttcaacatc tacttgtgg ccagaactc ttctggaaa ctcagccca atctcgaaa gagtcgcac gagcttgac caaaaaatc tacaactat gtcaaaacg gttatacac aataagagg cacaagggg ttgaagtcg cacactggc aagaagatt ttcttggat tatttggtc gaattgacg gaccgggag ggccgatgg actgacgaa gaattgacc aatgaaacc aggagtgtg attatggca gggagtgat gccacagct ttgaccctt tcatattgt cttgtaatg ctggcgatg tttcaagac atacaagac aaggtgtac gaggaattg tgttcggtt ttcggtgac agtgaccgg ttcgtcacc gtcgacgat ttgccccat ttgcagtac ttggatcgc ttcattaaa gagactttg agggttttc ccagtcacc tcaatgatc ggtcgcgaa ttgaccaca gatatgacc atagatggg cattttata ccaaaaggt acttcaatt ggattcccc attttgtat attcacagg aaccctgaa tattacccc gacccgctc aaattcgac ccggatcgt ttcctgccg gaagaagtt gccaaaaga cacccttgt acttttatt ccgttcagt tttgggccg aggaattgc attggttat aggtacgcg atgatgacc atgaaagtg attcttgca actctgctt aggagtttc aaaatggtg cacaccccg tataaagaa atttcagaa ttgaaaatt aagtttgat attgccacc aaagtcgac gaagggtat ccggtgcga atggagctt cgcaaaaat gtggcagct ggcactaaa tag
- >TC032539 RA
tcgaattag aactctaat aatgtattt cgtaactcc cggtctcat ttttacggt aatagcact ttggttgtt gtggtcatt cttgtggaa aaatttttt cgctacgaa gatcaaagg accatgggc ctttccgct catcggaag cgcctacgt cctgtttgg cggaacaca caatatttt ggaaaatac ggtccgagt ggtcagcaa gtaccggcc gttgtgtaa actgtggtt gggcaataa gttgacgat tttcccctc gaaccctga cgacatcca gatgatcct caacaggtc tttggagaa ggaggaggg ttataggta catcaagac tgtttttgg acacggctt gttcactag tcccgtaag cgagtggaa ggggcatcg gaaagtcat cgtttcgac tctcaacca gcaagtttt gaacagttt tatggacac gtacaatat ttacacgcg ggaattgat cgagaaact caaaattga caccaagga cgggcagca tatcgatat gttccccat tatgaccca gtgcacctt ggatatcat ctgcagctc catcatggg gacgcagat gaatgccat gaaggagga aagtcagtt tattatgtg gatgacaag agtggccga gcttgcaat cattcgcat gttcaacat ctacttgtg gccagaact cttctggaa actcagccc aatctcgaa agagtcgca cgagcttga ccaaaaaat ctacaacta tgtcaaaac ggttataca caataagag gcacaaggg gttgaagtc gcacactgg caagaagat tttcttgga ttatttggt cgaattgac ggaccggga gggccgatg gactgacga agaattgac caatgaaac caggagtgt gattatggc agggagtga tgccacagc tttgaccct ttcatattg tcttgtaat gctggcgat gtttcaaga catacaaga caaggtgta cgaggaatt gtgttcggt tttcggtga cagtgaccg gttcgtcac cgtcgacga tttgcccca tttgcagta cttggatcg cttcattaa agagacttt gagggtttt cccagtcac ctcaatgat cggtcgcga attgaccac agatatgac catagatgg gcattttat accaaaagg tacttcaat tggattccc cattttgta tattcacag gaaccctga atattaccc cgacccgct caaattcga cccggatcg tttcctgcc ggaagaagt tgccaaaag acacccttg tacttttat tccgttcag ttttgggcc gaggaattg cattggtta taggtacgc gatgatgac catgaaagt gattcttgc aactctgct taggagttt caaaatggt gcacacccc gtataaaga aatttcaga attgaaaat taagtttga tattgccac caaagtcga cgaagggta tccggtgcg aatggagct tcgcaaaaa tgtggcagc tggcactaa atagttgtg tagagttgg attttgatc agaaataaa cgatttcga gactc
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
