Status
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Gene information
General information
TC032539
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003768
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 17888353..17890124
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032539
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02722

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032539 RA
      MYFVTPGLI FTVIALWLL WSFLWKNFF ATKIKGPWA 
      FPLIGSAYV LFGGTHNIL ENTVRVVSK YRPLCKLWL 
      GNKLTIFPS NPDDIQMIL NRSLEKEEG YRYIKTVFG 
      HGLFTSPVS EWKGHRKVI VSTLNQQVL NSFMDTYNI 
      YTRELIEKL KIDTKDGQH IDMFPIMTQ CTLDIICSS 
      IMGTQMNAM KEESQFIMW MTRVAELAI IRMFNIYLW 
      PELFWKLSP ISKESHELD QKIYNYVKT VIHNKRHKG 
      LKSHTGKKI FLDYLVELT DREGRWTDE ELTNETRSV 
      IMAGSDATA LTLSYCLVM LAMFQDIQD KVYEELCSV 
      FGDSDRFVT VDDLPHLQY LDRFIKETL RVFPVTSMI 
      GRELTTDMT IDGHFIPKG TSIGFPILY IHRNPEYYP 
      DPLKFDPDR FLPEEVAKR HPCTFIPFS FGPRNCIGY 
      RYAMMTMKV ILATLLRSF KMVHTPYKE ISELKIKFD 
      IATKVDEGY PVRMELRKN VAAGTK
    CDS sequence
    • >TC032539 RA
      atgtatttc gtaactccc ggtctcatt tttacggta 
      atagcactt tggttgttg tggtcattc ttgtggaaa 
      aattttttc gctacgaag atcaaagga ccatgggcc 
      tttccgctc atcggaagc gcctacgtc ctgtttggc 
      ggaacacac aatattttg gaaaatacg gtccgagtg 
      gtcagcaag taccggccg ttgtgtaaa ctgtggttg 
      ggcaataag ttgacgatt ttcccctcg aaccctgac 
      gacatccag atgatcctc aacaggtct ttggagaag 
      gaggagggt tataggtac atcaagact gtttttgga 
      cacggcttg ttcactagt cccgtaagc gagtggaag 
      gggcatcgg aaagtcatc gtttcgact ctcaaccag 
      caagttttg aacagtttt atggacacg tacaatatt 
      tacacgcgg gaattgatc gagaaactc aaaattgac 
      accaaggac gggcagcat atcgatatg ttccccatt 
      atgacccag tgcaccttg gatatcatc tgcagctcc 
      atcatgggg acgcagatg aatgccatg aaggaggaa 
      agtcagttt attatgtgg atgacaaga gtggccgag 
      cttgcaatc attcgcatg ttcaacatc tacttgtgg 
      ccagaactc ttctggaaa ctcagccca atctcgaaa 
      gagtcgcac gagcttgac caaaaaatc tacaactat 
      gtcaaaacg gttatacac aataagagg cacaagggg 
      ttgaagtcg cacactggc aagaagatt ttcttggat 
      tatttggtc gaattgacg gaccgggag ggccgatgg 
      actgacgaa gaattgacc aatgaaacc aggagtgtg 
      attatggca gggagtgat gccacagct ttgaccctt 
      tcatattgt cttgtaatg ctggcgatg tttcaagac 
      atacaagac aaggtgtac gaggaattg tgttcggtt 
      ttcggtgac agtgaccgg ttcgtcacc gtcgacgat 
      ttgccccat ttgcagtac ttggatcgc ttcattaaa 
      gagactttg agggttttc ccagtcacc tcaatgatc 
      ggtcgcgaa ttgaccaca gatatgacc atagatggg 
      cattttata ccaaaaggt acttcaatt ggattcccc 
      attttgtat attcacagg aaccctgaa tattacccc 
      gacccgctc aaattcgac ccggatcgt ttcctgccg 
      gaagaagtt gccaaaaga cacccttgt acttttatt 
      ccgttcagt tttgggccg aggaattgc attggttat 
      aggtacgcg atgatgacc atgaaagtg attcttgca 
      actctgctt aggagtttc aaaatggtg cacaccccg 
      tataaagaa atttcagaa ttgaaaatt aagtttgat 
      attgccacc aaagtcgac gaagggtat ccggtgcga 
      atggagctt cgcaaaaat gtggcagct ggcactaaa 
      tag
    mRNA sequence
    • >TC032539 RA
      tcgaattag aactctaat aatgtattt cgtaactcc 
      cggtctcat ttttacggt aatagcact ttggttgtt 
      gtggtcatt cttgtggaa aaatttttt cgctacgaa 
      gatcaaagg accatgggc ctttccgct catcggaag 
      cgcctacgt cctgtttgg cggaacaca caatatttt 
      ggaaaatac ggtccgagt ggtcagcaa gtaccggcc 
      gttgtgtaa actgtggtt gggcaataa gttgacgat 
      tttcccctc gaaccctga cgacatcca gatgatcct 
      caacaggtc tttggagaa ggaggaggg ttataggta 
      catcaagac tgtttttgg acacggctt gttcactag 
      tcccgtaag cgagtggaa ggggcatcg gaaagtcat 
      cgtttcgac tctcaacca gcaagtttt gaacagttt 
      tatggacac gtacaatat ttacacgcg ggaattgat 
      cgagaaact caaaattga caccaagga cgggcagca 
      tatcgatat gttccccat tatgaccca gtgcacctt 
      ggatatcat ctgcagctc catcatggg gacgcagat 
      gaatgccat gaaggagga aagtcagtt tattatgtg 
      gatgacaag agtggccga gcttgcaat cattcgcat 
      gttcaacat ctacttgtg gccagaact cttctggaa 
      actcagccc aatctcgaa agagtcgca cgagcttga 
      ccaaaaaat ctacaacta tgtcaaaac ggttataca 
      caataagag gcacaaggg gttgaagtc gcacactgg 
      caagaagat tttcttgga ttatttggt cgaattgac 
      ggaccggga gggccgatg gactgacga agaattgac 
      caatgaaac caggagtgt gattatggc agggagtga 
      tgccacagc tttgaccct ttcatattg tcttgtaat 
      gctggcgat gtttcaaga catacaaga caaggtgta 
      cgaggaatt gtgttcggt tttcggtga cagtgaccg 
      gttcgtcac cgtcgacga tttgcccca tttgcagta 
      cttggatcg cttcattaa agagacttt gagggtttt 
      cccagtcac ctcaatgat cggtcgcga attgaccac 
      agatatgac catagatgg gcattttat accaaaagg 
      tacttcaat tggattccc cattttgta tattcacag 
      gaaccctga atattaccc cgacccgct caaattcga 
      cccggatcg tttcctgcc ggaagaagt tgccaaaag 
      acacccttg tacttttat tccgttcag ttttgggcc 
      gaggaattg cattggtta taggtacgc gatgatgac 
      catgaaagt gattcttgc aactctgct taggagttt 
      caaaatggt gcacacccc gtataaaga aatttcaga 
      attgaaaat taagtttga tattgccac caaagtcga 
      cgaagggta tccggtgcg aatggagct tcgcaaaaa 
      tgtggcagc tggcactaa atagttgtg tagagttgg 
      attttgatc agaaataaa cgatttcga gactc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY