| General information | |||
| TC002673 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 17903926..18002354 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC002673 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02721 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
Days post injection
- >TC002673 RA
MADEEVLFD DVYDLCEEI GRGPFSIVR RCIHRQTGQ QFAAKIVDV AKFTSSPGL STDDLKREA TICHMLKHP HIVELLETY SSEGMLYMV FEYMDGSDL CYEVVRRAS SGFVYSEAV ASHYMRQIL EALRYCHEN DIVHRDIKP ESILLATKE NSAPVKLGG FGVAVQLPD RHPANPGGR VGCPNFMAP EVIERRSYG KPVDIWGAG VLLHILLSG VLPFHGSGR RLMESICRG KLHLDSPQW ELISDSAKD LIQQMLTVD PKQRITIQE VLNHRWLRD RDKGLRIHL GESVEELKK FNARRKLKA VILSAVNSS KWYPYDETN SDSLSDFGD DEISACAVQ TILDSLDDI SCLQESQPQ ERAHLLALL EDTHLHILL ELYDRIASK VVTPIRAPP SDAVQRAKD AADALRELE HRRDTDHRD LQELRDLLC RPHFKALLQ AHDVVAHEI YGEDAMRVT PPPLLPYLN GGDDLDGPN GEVEMENVT RVRLVQFQK NTDEPMGIT LKMNEDGKC IVARIMHGG MIHRQATLH VGDEIREIN GISVMNQSV SALQKILRE ARGSVTFKI VPSYRSAPP PCEIFVRAQ FDYDPLEDD LIPCAQAGI AFKTGDILQ IISKDDHHW WQARKDNSA GSAGLIPSP ELQEWRAAC AAMEKTKQE QVNCSIFGR KKKQYKDKY LAKHNAVFD QLDLVTYEE VVKLPMTDP AFQRKTLVL LGAHGVGRR HIKNTLIAK HPDQYAYPI PHTTRQPRA DEENGRNYF FVSHDEMMA DIAANEYLE YGTHEDAMY GTKLDTIRK IHQEGKMAI LDVEPQALK VLRTAEFSP YVVFIAAPA LQNIADYDG SLERLAKES DMLRQAYGH YFDLTIVNN DIDETIAAL EAAIERVQT TPQWIPVSW VY
- >TC002673 RB
MADEEVLFD DVYDLCEEI GRGPFSIVR RCIHRQTGQ QFAAKIVDV AKFTSSPGL STDDLKREA TICHMLKHP HIVELLETY SSEGMLYMV FEYMDGSDL CYEVVRRAS SGFVYSEAV ASHYMRQIL EALRYCHEN DIVHRDIKP ESILLATKE NSAPVKLGG FGVAVQLPD RHPANPGGR VGCPNFMAP EVIERRSYG KPVDIWGAG VLLHILLSG VLPFHGSGR RLMESICRG KLHLDSPQW ELISDSAKD LIQQMLTVD PKQRITIQE VLNHRWLRD RDKGLRIHL GESVEELKK FNARRKLKA VILSAVNSS KWYPYDETN SDSLSDFGD DEISACAVQ TILDSLDDI SCLQESQPQ ERAHLLALL EDTHLHILL ELYDRIASK VVTPIRAPP SDAVQRAKD AADALRELE HRRDTDHRD LQELRDLLC RPHFKVSLL KFRCRPLVC CRPGAARSS ATLRPLVPF GAASAASLQ YCSGLPDRR RVCTIEATP TPDPAYARP QSAMTSRFP LVKDYYPPV SPLCPVSPG GVRDLWIGE ETKRSMPPH VYKYYLART RCYRRQSLR CASRSRGGS AAPSHLSSF SEYGVQSAC VLDPYGAGG WAPQPHYGG PPHWCEPRP GRSSDPVTP IGGPLRQVD SPFHAACPV HSPFRFRFI NGGPDYHPH HQVMNYSGV FSGRLLFGT LCQNWRGGK RVKVVHADL LTQLDGLLE GLGMKGGVG ALIVTKRD
- >TC002673 RA
atggccgac gaggaagtt ctcttcgac gacgtctac gatctctgc gaggagatc ggaagggga ccatttagc atagttaga cgatgtatt caccgccaa accggtcag caattcgct gcgaagatc gtagatgtc gccaaattc acttctagt cctggcctt agcacggat gatctgaag cgagaagcc acaatatgt cacatgctg aagcatccg cacatagtc gaattatta gaaacgtac tcgtccgaa gggatgtta tatatggtg ttcgaatac atggacggt tccgacctg tgttacgaa gtggtacgg agagcatcc tcgggattc gtttacagc gaggctgta gctagtcat tacatgcgc cagatcctc gaggcgttg cggtactgc cacgagaac gacatcgtc cacagggac atcaaaccg gagagtatc cttttagcc actaaggag aattcggcg cccgtaaaa ctcggcggt ttcggagtc gccgtccaa ttacccgat agacatccg gctaatcca ggaggccga gtgggatgt cccaacttc atggccccc gaagtgatc gagaggcga tcgtacggc aaacccgtg gacatctgg ggtgcgggt gtccttctc cacatcctt ctttccggc gttttgcct ttccatgga agcggccgt cgcctcatg gaatctata tgtcgcggc aaactgcac ttggattcg ccgcagtgg gagctcatt agtgatagt gcaaaagac ttaatacaa caaatgtta actgtggat cccaaacag aggataacg atacaggaa gttttaaat catagatgg ctgagggat cgcgataag ggattaagg attcatctc ggcgagtcg gtggaagaa ttaaaaaaa tttaacgcg aggagaaaa ttaaaagca gtgattttg agtgcggtc aattcgagt aaatggtat ccgtacgac gaaacgaat tcggactcg ctctcggac ttcggcgac gacgagatc tcggcgtgc gccgtccag acgatcctg gactcgctg gacgatatc tcctgcctt caagagtcg cagcctcaa gagcgcgcg caccttctg gccctgctc gaggacacc catcttcac atcctgctg gagttgtac gatcgcatc gcgagtaaa gttgtgacg ccgatcagg gcgccacct agcgacgcc gtacagagg gccaaagac gccgccgac gctctcagg gaactggaa catcgaagg gacacggac catagggat ctacaggag ttgagggat ctgctttgc cggccacat ttcaaggcc cttttacaa gctcatgat gttgtggcg cacgaaatt tacggcgaa gatgctatg cgggtcacg cccccgcct cttctgccg tacctcaac gggggcgac gatctggac ggtcccaac ggcgaggtg gaaatggag aacgtaacc agagtgagg ctggtgcag tttcagaaa aacaccgac gagccgatg ggcattacg ctgaagatg aacgaagac ggaaagtgt atagtggct aggataatg cacggaggg atgattcat cgacaagcg acgcttcat gtaggcgat gaaattaga gaaattaat ggaatttcc gttatgaac caatccgtt agtgctctc caaaaaatc ctcagggag gcaagaggt tcagtcacg tttaagatt gtaccgtct tatagaagt gcaccaccc ccttgcgag attttcgtt cgagcacaa ttcgattat gatccactt gaggacgat ttgattcct tgcgcccaa gctggtatc gccttcaaa accggagat atccttcaa attattagt aaagatgat catcactgg tggcaagcc cgcaaggac aactctgcg ggctctgcg ggcttgatc ccttcaccc gaattacaa gaatggagg gccgcttgc gccgccatg gaaaaaacg aaacaagaa caagtcaat tgctcaata ttcggtcgc aagaagaaa cagtacaag gataaatat ttggcaaag cacaatgct gtttttgac caattagat ttagtaact tatgaggaa gttgtgaaa ttgccaatg actgatcca gctttccag cgaaagacc ttagtcctc ctgggggct cacggggtc ggtagacgg catattaaa aatactcta atagctaaa catcccgat cagtatgct tatcctata ccacataca acgaggcaa cctagagcc gacgaggaa aacggccgc aattatttc ttcgtgtcg cacgacgaa atgatggcc gatatagcc gctaatgag tatttagaa tatggcacg cacgaagat gccatgtac ggaactaag ttagataca atccgtaaa atacatcaa gaaggtaaa atggccatt cttgatgtt gaacctcaa gcactcaaa gtgttgcgt actgctgag ttctcacca tatgttgtg ttcattgct gctccggct ttgcaaaac attgcagat tacgacggt agtttagaa cgactcgcc aaagaatct gacatgttg cgtcaggcc tacgggcac tacttcgac ttaacaatc gtaaacaat gatatcgac gagacgatc gcggccttg gaggcggcc atcgagcgc gtccagacc actccgcaa tggattccc gtttcttgg gtctactga
- >TC002673 RB
atggccgac gaggaagtt ctcttcgac gacgtctac gatctctgc gaggagatc ggaagggga ccatttagc atagttaga cgatgtatt caccgccaa accggtcag caattcgct gcgaagatc gtagatgtc gccaaattc acttctagt cctggcctt agcacggat gatctgaag cgagaagcc acaatatgt cacatgctg aagcatccg cacatagtc gaattatta gaaacgtac tcgtccgaa gggatgtta tatatggtg ttcgaatac atggacggt tccgacctg tgttacgaa gtggtacgg agagcatcc tcgggattc gtttacagc gaggctgta gctagtcat tacatgcgc cagatcctc gaggcgttg cggtactgc cacgagaac gacatcgtc cacagggac atcaaaccg gagagtatc cttttagcc actaaggag aattcggcg cccgtaaaa ctcggcggt ttcggagtc gccgtccaa ttacccgat agacatccg gctaatcca ggaggccga gtgggatgt cccaacttc atggccccc gaagtgatc gagaggcga tcgtacggc aaacccgtg gacatctgg ggtgcgggt gtccttctc cacatcctt ctttccggc gttttgcct ttccatgga agcggccgt cgcctcatg gaatctata tgtcgcggc aaactgcac ttggattcg ccgcagtgg gagctcatt agtgatagt gcaaaagac ttaatacaa caaatgtta actgtggat cccaaacag aggataacg atacaggaa gttttaaat catagatgg ctgagggat cgcgataag ggattaagg attcatctc ggcgagtcg gtggaagaa ttaaaaaaa tttaacgcg aggagaaaa ttaaaagca gtgattttg agtgcggtc aattcgagt aaatggtat ccgtacgac gaaacgaat tcggactcg ctctcggac ttcggcgac gacgagatc tcggcgtgc gccgtccag acgatcctg gactcgctg gacgatatc tcctgcctt caagagtcg cagcctcaa gagcgcgcg caccttctg gccctgctc gaggacacc catcttcac atcctgctg gagttgtac gatcgcatc gcgagtaaa gttgtgacg ccgatcagg gcgccacct agcgacgcc gtacagagg gccaaagac gccgccgac gctctcagg gaactggaa catcgaagg gacacggac catagggat ctacaggag ttgagggat ctgctttgc cggccacat ttcaaggtg tcgctttta aaatttcgc tgccgcccc cttgtttgt tgtcgtccg ggggcggct cgcagcagc gccactctg cggccgtta gtgcctttc ggcgccgct tccgcagcc tcgctccag tactgcagc gggctcccg gaccgccgc cgcgtctgc acgatcgaa gcgacgccc acccccgat ccggcctat gcacggcct caaagtgct atgactagt cgctttcca ctggtgaaa gactactac cctccggtg tctcctcta tgtcctgtg tctcccgga ggggtccgg gacctctgg atcggggag gagacgaag cgttctatg cctccgcac gtctataag tattacctg gcgcgcaca cgctgctat cgacgacaa agcctccgg tgtgcatcg cgctctcga ggcggcagc gccgctccc tcgcacttg tcctcattt tcggagtac ggtgttcaa agtgcgtgc gtcctggat ccgtacggg gccggcgga tgggccccc cagccgcac tatgggggc cctccccat tggtgcgaa ccgcggcct ggcaggtcc tcagaccct gtgactccg atcgggggc cccttgaga caagtggat agcccgttt cacgccgct tgtccggtg cacagccct ttcaggttc aggttcatc aacgggggg ccggattac catccgcat catcaggtg atgaattat tcaggggtt ttttcgggg cggctgctc tttggtaca ctgtgccaa aattggagg ggcggcaag cgtgtcaag gtcgtccac gctgactta ttgacacaa ttagacggt ttattagag ggattaggg atgaaaggc ggcgtggga gcactaatt gttacaaaa cgggattaa
- >TC002673 RA
aaagttaac gcgaatgga ccaatcact ttttttcat gtctttgtt gtggcacca ttccgacct gtcaaatat catcgaaaa ttaatcaaa cccgtttta atgaaactc gcccctgga aatcgatgc ccgcatgtg aaacccccg cctgtccct atttagtag tttaaaacc ggtgccgcg ttattaaag tgcgaagat aggttatgt tgtcagcct gacatttgc gtgtgacaa tccaggacc gggtttcct gtcggcggc cgcacccac cgtatctaa atcagtcat caaactcca aacaagccg ctttggagg tgttaaaag tccagtccg tagtgttaa aacccgcgg cgcgacgct gcgcatgtg ttttaggcg tgatcccgg ccggtgttg cgcctgttc ctgtcacag ctgtcagtt tgtggctgt ttattagcg tgttgttgg gtgtgtgcg tcgtgcggc ggtgaaaac gtccgattg tcgcggtgt tcgtttgcg gagtcctgt tcgccgccc ccagttggg gccagagtg gtaggcaac ccccatggg cctgtgctg aacggcttg cctagctgc cggcgtcgc cgccccaca ggcatggtc taacgatgg ccgacgagg aagttctct tcgacgacg tctacgatc tctgcgagg agatcggaa ggggaccat ttagcatag ttagacgat gtattcacc gccaaaccg gtcagcaat tcgctgcga agatcgtag atgtcgcca aattcactt ctagtcctg gccttagca cggatgatc tgaagcgag aagccacaa tatgtcaca tgctgaagc atccgcaca tagtcgaat tattagaaa cgtactcgt ccgaaggga tgttatata tggtgttcg aatacatgg acggttccg acctgtgtt acgaagtgg tacggagag catcctcgg gattcgttt acagcgagg ctgtagcta gtcattaca tgcgccaga tcctcgagg cgttgcggt actgccacg agaacgaca tcgtccaca gggacatca aaccggaga gtatccttt tagccacta aggagaatt cggcgcccg taaaactcg gcggtttcg gagtcgccg tccaattac ccgatagac atccggcta atccaggag gccgagtgg gatgtccca acttcatgg cccccgaag tgatcgaga ggcgatcgt acggcaaac ccgtggaca tctggggtg cgggtgtcc ttctccaca tccttcttt ccggcgttt tgcctttcc atggaagcg gccgtcgcc tcatggaat ctatatgtc gcggcaaac tgcacttgg attcgccgc agtgggagc tcattagtg atagtgcaa aagacttaa tacaacaaa tgttaactg tggatccca aacagagga taacgatac aggaagttt taaatcata gatggctga gggatcgcg ataagggat taaggattc atctcggcg agtcggtgg aagaattaa aaaaattta acgcgagga gaaaattaa aagcagtga ttttgagtg cggtcaatt cgagtaaat ggtatccgt acgacgaaa cgaattcgg actcgctct cggacttcg gcgacgacg agatctcgg cgtgcgccg tccagacga tcctggact cgctggacg atatctcct gccttcaag agtcgcagc ctcaagagc gcgcgcacc ttctggccc tgctcgagg acacccatc ttcacatcc tgctggagt tgtacgatc gcatcgcga gtaaagttg tgacgccga tcagggcgc cacctagcg acgccgtac agagggcca aagacgccg ccgacgctc tcagggaac tggaacatc gaagggaca cggaccata gggatctac aggagttga gggatctgc tttgccggc cacatttca aggcccttt tacaagctc atgatgttg tggcgcacg aaatttacg gcgaagatg ctatgcggg tcacgcccc cgcctcttc tgccgtacc tcaacgggg gcgacgatc tggacggtc ccaacggcg aggtggaaa tggagaacg taaccagag tgaggctgg tgcagtttc agaaaaaca ccgacgagc cgatgggca ttacgctga agatgaacg aagacggaa agtgtatag tggctagga taatgcacg gagggatga ttcatcgac aagcgacgc ttcatgtag gcgatgaaa ttagagaaa ttaatggaa tttccgtta tgaaccaat ccgttagtg ctctccaaa aaatcctca gggaggcaa gaggttcag tcacgttta agattgtac cgtcttata gaagtgcac caccccctt gcgagattt tcgttcgag cacaattcg attatgatc cacttgagg acgatttga ttccttgcg cccaagctg gtatcgcct tcaaaaccg gagatatcc ttcaaatta ttagtaaag atgatcatc actggtggc aagcccgca aggacaact ctgcgggct ctgcgggct tgatccctt cacccgaat tacaagaat ggagggccg cttgcgccg ccatggaaa aaacgaaac aagaacaag tcaattgct caatattcg gtcgcaaga agaaacagt acaaggata aatatttgg caaagcaca atgctgttt ttgaccaat tagatttag taacttatg aggaagttg tgaaattgc caatgactg atccagctt tccagcgaa agaccttag tcctcctgg gggctcacg gggtcggta gacggcata ttaaaaata ctctaatag ctaaacatc ccgatcagt atgcttatc ctataccac atacaacga ggcaaccta gagccgacg aggaaaacg gccgcaatt atttcttcg tgtcgcacg acgaaatga tggccgata tagccgcta atgagtatt tagaatatg gcacgcacg aagatgcca tgtacggaa ctaagttag atacaatcc gtaaaatac atcaagaag gtaaaatgg ccattcttg atgttgaac ctcaagcac tcaaagtgt tgcgtactg ctgagttct caccatatg ttgtgttca ttgctgctc cggctttgc aaaacattg cagattacg acggtagtt tagaacgac tcgccaaag aatctgaca tgttgcgtc aggcctacg ggcactact tcgacttaa caatcgtaa acaatgata tcgacgaga cgatcgcgg ccttggagg cggccatcg agcgcgtcc agaccactc cgcaatgga ttcccgttt cttgggtct actgacaac aaccttcat tggaattcg gctcgcttc agaacttct gagccgcgc tttcaccgg aatttattg aagtgaatt aacagtgaa agaagctgc accacacac tcacacaca cccgacaca gagagtagt ctgaaaaaa tgaaaaaat aaatcgcta gtgttttgt gttttttcg tttttcttt tccaaagac acgctgata ccaaagcca atttgactc gtttgctaa gtataggat tagaaatag cgatgttga atgtaacgt ttcgttcga cacccggtc ttcatcagt agtttcata gtttctaaa cctacacaa cgcacaact caatgaaat tcaaacaac gtaacgaaa cgataattt gcttcaata taagaaaaa atatagcaa tacgtctga aagtattac aaacttaat cgtaattat tatataatg tgatttaat gatgttaaa gtgctcaca agtactgtt aaaagcgat aactcgacg acgtcaaaa ttctgatta cgactcatt tcttgcttt gatttgatt ggttcgccg gcttgttac taaaaacac agcttcctt gatcactga agaccggtg tcagcccta gtcattgat cgaggaagc tgtgaaaaa cacatatcc gattcgagg atgactaga aaatttgat gacgggaaa ccaaattct agattacga gatattgta ctataaagc ttttcaatt tttgatagt gagatgaaa ttgttctat tttgaggca gtggagttt cattttgat cattttctg ctgtttata tatttatag ataagtgcc attgtcgat ttatgtaaa gaagtttaa ttatttcag ttatgattg aacactaaa aattaaaaa aacgaaaaa cacattcat taaacttat acaagtgat tgcttcctc aaataagtg tacctacct acttaaaaa atgaactaa agggtattc ctaacataa tttgataaa aagtagtgt agcgtttta aaagtttgc atttatttg aaacgttag acaatgtag gttcaatat ttctgaaat aaaatgtaa tataatgc
- >TC002673 RB
aaagttaac gcgaatgga ccaatcact ttttttcat gtctttgtt gtggcacca ttccgacct gtcaaatat catcgaaaa ttaatcaaa cccgtttta atgaaactc gcccctgga aatcgatgc ccgcatgtg aaacccccg cctgtccct atttagtag tttaaaacc ggtgccgcg ttattaaag tgcgaagat aggttatgt tgtcagcct gacatttgc gtgtgacaa tccaggacc gggtttcct gtcggcggc cgcacccac cgtatctaa atcagtcat caaactcca aacaagccg ctttggagg tgttaaaag tccagtccg tagtgttaa aacccgcgg cgcgacgct gcgcatgtg ttttaggcg tgatcccgg ccggtgttg cgcctgttc ctgtcacag ctgtcagtt tgtggctgt ttattagcg tgttgttgg gtgtgtgcg tcgtgcggc ggtgaaaac gtccgattg tcgcggtgt tcgtttgcg gagtcctgt tcgccgccc ccagttggg gccagagtg gtaggcaac ccccatggg cctgtgctg aacggcttg cctagctgc cggcgtcgc cgccccaca ggcatggtc taacgatgg ccgacgagg aagttctct tcgacgacg tctacgatc tctgcgagg agatcggaa ggggaccat ttagcatag ttagacgat gtattcacc gccaaaccg gtcagcaat tcgctgcga agatcgtag atgtcgcca aattcactt ctagtcctg gccttagca cggatgatc tgaagcgag aagccacaa tatgtcaca tgctgaagc atccgcaca tagtcgaat tattagaaa cgtactcgt ccgaaggga tgttatata tggtgttcg aatacatgg acggttccg acctgtgtt acgaagtgg tacggagag catcctcgg gattcgttt acagcgagg ctgtagcta gtcattaca tgcgccaga tcctcgagg cgttgcggt actgccacg agaacgaca tcgtccaca gggacatca aaccggaga gtatccttt tagccacta aggagaatt cggcgcccg taaaactcg gcggtttcg gagtcgccg tccaattac ccgatagac atccggcta atccaggag gccgagtgg gatgtccca acttcatgg cccccgaag tgatcgaga ggcgatcgt acggcaaac ccgtggaca tctggggtg cgggtgtcc ttctccaca tccttcttt ccggcgttt tgcctttcc atggaagcg gccgtcgcc tcatggaat ctatatgtc gcggcaaac tgcacttgg attcgccgc agtgggagc tcattagtg atagtgcaa aagacttaa tacaacaaa tgttaactg tggatccca aacagagga taacgatac aggaagttt taaatcata gatggctga gggatcgcg ataagggat taaggattc atctcggcg agtcggtgg aagaattaa aaaaattta acgcgagga gaaaattaa aagcagtga ttttgagtg cggtcaatt cgagtaaat ggtatccgt acgacgaaa cgaattcgg actcgctct cggacttcg gcgacgacg agatctcgg cgtgcgccg tccagacga tcctggact cgctggacg atatctcct gccttcaag agtcgcagc ctcaagagc gcgcgcacc ttctggccc tgctcgagg acacccatc ttcacatcc tgctggagt tgtacgatc gcatcgcga gtaaagttg tgacgccga tcagggcgc cacctagcg acgccgtac agagggcca aagacgccg ccgacgctc tcagggaac tggaacatc gaagggaca cggaccata gggatctac aggagttga gggatctgc tttgccggc cacatttca aggtgtcgc ttttaaaat ttcgctgcc gcccccttg tttgttgtc gtccggggg cggctcgca gcagcgcca ctctgcggc cgttagtgc ctttcggcg ccgcttccg cagcctcgc tccagtact gcagcgggc tcccggacc gccgccgcg tctgcacga tcgaagcga cgcccaccc ccgatccgg cctatgcac ggcctcaaa gtgctatga ctagtcgct ttccactgg tgaaagact actaccctc cggtgtctc ctctatgtc ctgtgtctc ccggagggg tccgggacc tctggatcg gggaggaga cgaagcgtt ctatgcctc cgcacgtct ataagtatt acctggcgc gcacacgct gctatcgac gacaaagcc tccggtgtg catcgcgct ctcgaggcg gcagcgccg ctccctcgc acttgtcct cattttcgg agtacggtg ttcaaagtg cgtgcgtcc tggatccgt acggggccg gcggatggg ccccccagc cgcactatg ggggccctc cccattggt gcgaaccgc ggcctggca ggtcctcag accctgtga ctccgatcg ggggcccct tgagacaag tggatagcc cgtttcacg ccgcttgtc cggtgcaca gccctttca ggttcaggt tcatcaacg gggggccgg attaccatc cgcatcatc aggtgatga attattcag gggtttttt cggggcggc tgctctttg gtacactgt gccaaaatt ggaggggcg gcaagcgtg tcaaggtcg tccacgctg acttattga cacaattag acggtttat tagagggat tagggatga aaggcggcg tgggagcac taattgtta caaaacggg attaaatct tgttgacgt gggggccac atttactcc aatgggtat ttttgagta tacaaaatt ttcgacggg agtgtttgt aggccttgc ctagatgga tgtatctct catgtttac atgagatga taagccttt gtacacttc ataaccgct ctgatgaca taaatagag ccctcgaca taattaatg gcgcttttc aatacattg aagaaaaat tt
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