Status
Please wait ...
Gene information
General information
TC010518 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 14539492..14558534
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010518
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02719

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010518 RA
      MPKEQYRET DVARKISHI SFGIESAES MQQQSHIHV 
      VAKNLYNQD VQRTPIPYG VLDKRLGTN SKESPCQTC 
      GKNINSCVG HFGYIDLEL PVYHVGYFR SIISILQTI 
      CKNCARVLL SEEEKNQYR IRLSNEHLS YMTKKAIRK 
      KIIEKCRKL TKCPYCKES NGFVKKLTA SKGSTGGSV 
      LKIVCEKNR GSDKETVLK EHIEKFNSA IDVNPEIKN 
      ALTANSAVS QILTPIDVL KLFERIPQS DIPLLAMDS 
      RRSQPKDLI FTRMLVPPV SIRPSVVSD LKAGTNEDD 
      LTMRQSEII FINDVIKKH KLSGASVNM YQEGWDFLQ 
      LQTALYINS ELSGIPLAM MPKKPGRGL VQRLKGKQG 
      RFRGNLSGK RVDFSSRTV ISPDPNLQI DQVGVPLHI 
      AKILTFPER VIHANIDVM RQLVINGPD KWPGANYVQ 
      QKGSAFKKF LKYGNREKL AQELKLGDL VERHLHNDD 
      VVLFNRQPS LHKLSIMAH RTKVHQHRT FRFNECVCN 
      PYNADFDGD EMNLHLPQT EEAKAEALI LMGNKNNLV 
      TPRNGELLI AATQDFLTG GYLLTKKDT FLDLAHASQ 
      LAATLLAEN DTNMQIDLP TPCILKPRR LWSGKQICS 
      LIFRPNKKC PVKANLEAK GKAYTKNRE LCVKDSYVL 
      IRNSELIAG TLDKSHLGS GGKGNNIFY VILRDFGSE 
      YAIKSMWRL AKLTSYYLM NSGFSIGIG DVTPSKGLL 
      RRKNKLLED GYTKCDEYI RQMADGRLQ CQPGCTAEE 
      TLEAVILKE LSVIREHAG QACLAELHP TNSPLIMAL 
      SGSKGSFIN ISQMIACVG QQALNGKRV PDGFENRSL 
      PHFPHHSKT PEAKGFVEN SFYTGLTPT EFFFHTMGG 
      REGLVDTAV KTAETGYMQ RRLVKSLED LVVHYDGSV 
      RNAEGDIVQ VNYGGDGLD PTYMEGKDC PVDFDRVLR 
      HVQAKCPYR DETILNSVQ IRRATKTFL ETDALNECS 
      EEFKAELKN FMDKVALRV ENIYNKFGD GKVVKEIER 
      LTCSQLVTF LQICGSKYT RSIIEPGTA VGALAAQSI 
      GEPGTQMTL KTFHFAGVA SMNITQGVP RIKEIINAS 
      KDISTPIIT ASLTNPRDI GFAREVKAR IERTTLGEV 
      ATFIDEIYT QYDCFLLIQ LNYDRIKLL KLEINAETV 
      RYSICTSKL KLKPNEVRV ESETIITVS PSGSKHTHR 
      FNFSLNELK AKLPSVVVK GLPTVNRAV IAKEEKGGE 
      TLYNLCVEG DNLREVIAT YGVLGTKTV SNNIMEVYH 
      TLGIEAARE TIMSEIKMV MENHGMSVD YRHIMLLAA 
      QMTHTGEVL GITRYGLSK MKQSILNLA SFEKTADHL 
      FDAAYYGQV DKINGVSEN IIMGMPAPI GTGIFKLLH 
      KPHHIEENP PSEPLLFET AIAK
    CDS sequence
    • >TC010518 RA
      atgccaaaa gaacaatac cgagaaact gatgtagcg 
      agaaaaatc tcgcacatc agtttcggg attgagagt 
      gcagagtcg atgcaacaa caatcccac attcacgtt 
      gtagctaaa aacttgtat aatcaggat gttcaacgc 
      actccaatt ccttacgga gtgctagac aagagactg 
      ggcacaaac tcgaaagaa tcgccttgt cagacatgc 
      gggaaaaac ataaattcg tgcgttggc cattttggg 
      tacattgac ttggaatta cctgtgtat catgtgggg 
      tactttaga agcattatt agcattctt caaacaatt 
      tgtaagaat tgtgcccgt gttctcctt tccgaggaa 
      gaaaaaaat caataccga atacgtctc tctaatgaa 
      catctgagt tatatgact aaaaaagct ataaggaag 
      aaaatcatt gagaaatgc agaaagttg actaagtgt 
      ccctactgt aaggaatct aatggcttt gtaaaaaag 
      ttgacagcc agtaaaggg agcactggg ggatctgtg 
      ttaaagata gtgtgtgaa aaaaataga gggagtgac 
      aaggaaaca gtgttgaaa gaacacatc gaaaagttc 
      aattcagcg attgatgtt aatccagag atcaaaaat 
      gcattaacg gccaattct gctgtctcg caaattctt 
      actccaatc gatgtttta aaattattt gagagaatt 
      ccacaaagt gatattcca ttattggca atggactca 
      aggaggtca caaccaaaa gatttaatt tttactaga 
      atgctagtc cctcctgtg tcaattcgc ccctctgtg 
      gtatcggac ttgaaggct gggaccaat gaggacgat 
      ttaactatg agacaatct gaaataatc tttatcaat 
      gatgtaatc aaaaaacac aaattgtca ggagcatct 
      gttaatatg tatcaagag ggttgggac tttcttcag 
      ctgcagact gctctttat attaacagt gaactttcc 
      ggaattccc ctagcaatg atgccaaaa aaaccgggc 
      cgtggtctt gtgcaacgc cttaaaggc aaacaaggc 
      cgtttccgc ggcaatctc tccggcaaa cgagtcgac 
      ttttcttcg agaacagtt atttcccct gatccgaat 
      ttacaaatc gatcaagtc ggtgttcct ctacacatc 
      gccaaaatc ttgactttt cctgaacgg gtgattcac 
      gcaaatatc gatgttatg agacaattg gtgatcaac 
      ggacctgac aagtggcct ggggctaat tacgttcaa 
      cagaaaggc tctgctttt aaaaagttt cttaaatac 
      ggaaacaga gaaaaacta gcacaggaa ttgaaactg 
      ggggatttg gttgaacgg caccttcac aacgacgat 
      gtcgtctta tttaatcgt caaccgagt ttgcacaag 
      ttgtcgatt atggcgcac aggaccaaa gtccatcaa 
      catcgaacg ttcaggttc aacgagtgc gtttgtaat 
      ccctacaat gcggatttt gacggcgac gaaatgaat 
      ttgcatttg cctcaaact gaagaggct aaagcggaa 
      gcgttaatt ttaatgggg aataaaaat aatctagtg 
      acgccacga aacggtgaa ctattgatc gcagcaacc 
      caggatttt ttgaccggt ggctactta ttgaccaaa 
      aaagatacg tttctggat ttagcacac gcatctcaa 
      ttagccgcc acgctattg gctgagaac gacacgaat 
      atgcaaatt gatttacca acaccctgt attttaaag 
      ccaagacgt ttgtggtct ggcaagcaa atttgtagt 
      cttattttc cgacctaat aagaaatgt ccagtcaag 
      gcaaatctt gaagcaaag ggcaaggcc tacacaaaa 
      aatagagag ctatgcgtt aaagactca tacgttttg 
      atcaggaat tccgaactt atcgctgga acgctggat 
      aaaagccac ttgggaagt ggaggcaaa ggcaataac 
      attttttac gtaattttg agagacttt ggctcagaa 
      tatgcaata aaatcaatg tggagatta gctaagctt 
      acctcttat tatttaatg aatagtgga ttcagtatc 
      gggataggc gacgtcact ccaagtaaa gggttactg 
      agacgaaag aacaagttg ttagaggat ggatatact 
      aaatgtgac gagtatatt cgtcaaatg gccgacggt 
      aggctccag tgccaaccg ggatgtacg gctgaggag 
      actttagaa gctgttatt ttgaaagaa ttatcagtt 
      attcgtgaa catgcaggt caggcgtgt ctcgcagaa 
      ctgcatcca accaatagt ccacttatt atggcgtta 
      tcgggttcg aaggggtct tttatcaat atttctcag 
      atgattgct tgtgtgggt caacaggct ttgaatggg 
      aaacgagtc cctgatgga tttgaaaat agatctctg 
      ccacatttt ccacatcac tcgaaaaca ccagaagct 
      aaaggcttt gttgaaaat agtttttac actggacta 
      acacctacg gaattcttt ttccataca atgggcggc 
      cgtgagggt cttgtcgac acagcagtt aaaactgcg 
      gaaactggc tatatgcag agaaggctg gtaaagtcg 
      ttggaagac ttggtggtg cactacgat ggttctgtc 
      agaaatgcg gaaggtgat atagttcag gtgaattac 
      ggaggtgat ggtttagac ccgacttac atggaaggt 
      aaagactgc ccagttgat tttgaccgt gttctgcgc 
      catgtccag gcgaaatgt ccttaccga gacgaaacg 
      atcctgaac agtgttcaa atcagacga gctactaaa 
      actttcctt gaaacggat gcactaaac gagtgtagc 
      gaggaattt aaagcggaa ctaaagaac ttcatggac 
      aaggttgcg ctaagagtg gaaaacata tacaataaa 
      ttcggggac ggaaaagtg gtgaaagaa atagaaaga 
      ctaacatgt agccagttg gtgacgttc cttcaaatt 
      tgcggttca aaatacacc aggtctatc attgaaccg 
      ggaactgca gttggtgct ttagctgct caaagtatt 
      ggtgaaccc ggaacacaa atgacactc aaaactttc 
      cattttgct ggagttgca agcatgaat attacacag 
      ggtgttccg agaatcaag gagattatc aatgccagt 
      aaggacatc agcacgcct ataattacc gccagcctg 
      accaaccca cgagatatc ggttttgct cgggaagtg 
      aaagcccga atcgaaagg acaaccctt ggcgaggtg 
      gcaacattt attgatgaa atttacacc cagtacgac 
      tgcttcctt ttaattcaa ttgaattac gaccgcatt 
      aaattattg aaacttgaa ataaatgcc gagactgtc 
      cgttacagt atttgcacc tcaaagttg aaacttaaa 
      ccaaacgaa gttcgcgtc gaaagtgaa acaattatc 
      acagtgtcc cccagtggt agtaaacat acacaccga 
      tttaatttt tcattgaat gagcttaag gcaaaattg 
      cccagtgtt gttgttaaa gggctgcca accgtaaat 
      cgagcagtc attgccaaa gaggagaaa ggaggcgaa 
      actctgtac aatttatgt gttgagggt gataattta 
      agggaagtt atcgctacg tatggtgtg ctggggaca 
      aagactgtc tccaataat atcatggaa gtgtaccat 
      actttggga atagaagcc gcaagagaa accattatg 
      tccgaaatt aaaatggta atggaaaac catggtatg 
      agcgtggat tatcgccac attatgtta ctcgcggcc 
      caaatgaca cacacgggg gaagttctg ggcatcaca 
      cgatatggt ttatccaaa atgaagcag tccatacta 
      aacttggct tcttttgag aaaaccgct gatcatttg 
      tttgatgct gcttattac ggccaggtt gacaaaata 
      aacggcgtt tcagaaaat attataatg ggaatgcct 
      gcacctata ggaaccgga attttcaaa ctcctccac 
      aagccacac cacatcgag gaaaatcca ccatcagaa 
      cctttacta tttgaaact gcgattgcc aagtaa
    mRNA sequence
    • >TC010518 RA
      atagttcta tgcgcgcag cgattgtct ggtcgactg 
      taatattca tggtgccag cacctgtct tcacagata 
      tagtttagg ttatgttgt gtttcaaaa aagtgaggt 
      tatttaagg cataattgt tatattttt aagcggttc 
      tgctttcaa agttcgttg gtaacacaa ctgaacatt 
      tgtaaacca gggcgagca caatgccaa aagaacaat 
      accgagaaa ctgatgtag cgagaaaaa tctcgcaca 
      tcagtttcg ggattgaga gtgcagagt cgatgcaac 
      aacaatccc acattcacg ttgtagcta aaaacttgt 
      ataatcagg atgttcaac gcactccaa ttccttacg 
      gagtgctag acaagagac tgggcacaa actcgaaag 
      aatcgcctt gtcagacat gcgggaaaa acataaatt 
      cgtgcgttg gccattttg ggtacattg acttggaat 
      tacctgtgt atcatgtgg ggtacttta gaagcatta 
      ttagcattc ttcaaacaa tttgtaaga attgtgccc 
      gtgttctcc tttccgagg aagaaaaaa atcaatacc 
      gaatacgtc tctctaatg aacatctga gttatatga 
      ctaaaaaag ctataagga agaaaatca ttgagaaat 
      gcagaaagt tgactaagt gtccctact gtaaggaat 
      ctaatggct ttgtaaaaa agttgacag ccagtaaag 
      ggagcactg ggggatctg tgttaaaga tagtgtgtg 
      aaaaaaata gagggagtg acaaggaaa cagtgttga 
      aagaacaca tcgaaaagt tcaattcag cgattgatg 
      ttaatccag agatcaaaa atgcattaa cggccaatt 
      ctgctgtct cgcaaattc ttactccaa tcgatgttt 
      taaaattat ttgagagaa ttccacaaa gtgatattc 
      cattattgg caatggact caaggaggt cacaaccaa 
      aagatttaa tttttacta gaatgctag tccctcctg 
      tgtcaattc gcccctctg tggtatcgg acttgaagg 
      ctgggacca atgaggacg atttaacta tgagacaat 
      ctgaaataa tctttatca atgatgtaa tcaaaaaac 
      acaaattgt caggagcat ctgttaata tgtatcaag 
      agggttggg actttcttc agctgcaga ctgctcttt 
      atattaaca gtgaacttt ccggaattc ccctagcaa 
      tgatgccaa aaaaaccgg gccgtggtc ttgtgcaac 
      gccttaaag gcaaacaag gccgtttcc gcggcaatc 
      tctccggca aacgagtcg acttttctt cgagaacag 
      ttatttccc ctgatccga atttacaaa tcgatcaag 
      tcggtgttc ctctacaca tcgccaaaa tcttgactt 
      ttcctgaac gggtgattc acgcaaata tcgatgtta 
      tgagacaat tggtgatca acggacctg acaagtggc 
      ctggggcta attacgttc aacagaaag gctctgctt 
      ttaaaaagt ttcttaaat acggaaaca gagaaaaac 
      tagcacagg aattgaaac tgggggatt tggttgaac 
      ggcaccttc acaacgacg atgtcgtct tatttaatc 
      gtcaaccga gtttgcaca agttgtcga ttatggcgc 
      acaggacca aagtccatc aacatcgaa cgttcaggt 
      tcaacgagt gcgtttgta atccctaca atgcggatt 
      ttgacggcg acgaaatga atttgcatt tgcctcaaa 
      ctgaagagg ctaaagcgg aagcgttaa ttttaatgg 
      ggaataaaa ataatctag tgacgccac gaaacggtg 
      aactattga tcgcagcaa cccaggatt ttttgaccg 
      gtggctact tattgacca aaaaagata cgtttctgg 
      atttagcac acgcatctc aattagccg ccacgctat 
      tggctgaga acgacacga atatgcaaa ttgatttac 
      caacaccct gtattttaa agccaagac gtttgtggt 
      ctggcaagc aaatttgta gtcttattt tccgaccta 
      ataagaaat gtccagtca aggcaaatc ttgaagcaa 
      agggcaagg cctacacaa aaaatagag agctatgcg 
      ttaaagact catacgttt tgatcagga attccgaac 
      ttatcgctg gaacgctgg ataaaagcc acttgggaa 
      gtggaggca aaggcaata acatttttt acgtaattt 
      tgagagact ttggctcag aatatgcaa taaaatcaa 
      tgtggagat tagctaagc ttacctctt attatttaa 
      tgaatagtg gattcagta tcgggatag gcgacgtca 
      ctccaagta aagggttac tgagacgaa agaacaagt 
      tgttagagg atggatata ctaaatgtg acgagtata 
      ttcgtcaaa tggccgacg gtaggctcc agtgccaac 
      cgggatgta cggctgagg agactttag aagctgtta 
      ttttgaaag aattatcag ttattcgtg aacatgcag 
      gtcaggcgt gtctcgcag aactgcatc caaccaata 
      gtccactta ttatggcgt tatcgggtt cgaaggggt 
      cttttatca atatttctc agatgattg cttgtgtgg 
      gtcaacagg ctttgaatg ggaaacgag tccctgatg 
      gatttgaaa atagatctc tgccacatt ttccacatc 
      actcgaaaa caccagaag ctaaaggct ttgttgaaa 
      atagttttt acactggac taacaccta cggaattct 
      ttttccata caatgggcg gccgtgagg gtcttgtcg 
      acacagcag ttaaaactg cggaaactg gctatatgc 
      agagaaggc tggtaaagt cgttggaag acttggtgg 
      tgcactacg atggttctg tcagaaatg cggaaggtg 
      atatagttc aggtgaatt acggaggtg atggtttag 
      acccgactt acatggaag gtaaagact gcccagttg 
      attttgacc gtgttctgc gccatgtcc aggcgaaat 
      gtccttacc gagacgaaa cgatcctga acagtgttc 
      aaatcagac gagctacta aaactttcc ttgaaacgg 
      atgcactaa acgagtgta gcgaggaat ttaaagcgg 
      aactaaaga acttcatgg acaaggttg cgctaagag 
      tggaaaaca tatacaata aattcgggg acggaaaag 
      tggtgaaag aaatagaaa gactaacat gtagccagt 
      tggtgacgt tccttcaaa tttgcggtt caaaataca 
      ccaggtcta tcattgaac cgggaactg cagttggtg 
      ctttagctg ctcaaagta ttggtgaac ccggaacac 
      aaatgacac tcaaaactt tccattttg ctggagttg 
      caagcatga atattacac agggtgttc cgagaatca 
      aggagatta tcaatgcca gtaaggaca tcagcacgc 
      ctataatta ccgccagcc tgaccaacc cacgagata 
      tcggttttg ctcgggaag tgaaagccc gaatcgaaa 
      ggacaaccc ttggcgagg tggcaacat ttattgatg 
      aaatttaca cccagtacg actgcttcc ttttaattc 
      aattgaatt acgaccgca ttaaattat tgaaacttg 
      aaataaatg ccgagactg tccgttaca gtatttgca 
      cctcaaagt tgaaactta aaccaaacg aagttcgcg 
      tcgaaagtg aaacaatta tcacagtgt cccccagtg 
      gtagtaaac atacacacc gatttaatt tttcattga 
      atgagctta aggcaaaat tgcccagtg ttgttgtta 
      aagggctgc caaccgtaa atcgagcag tcattgcca 
      aagaggaga aaggaggcg aaactctgt acaatttat 
      gtgttgagg gtgataatt taagggaag ttatcgcta 
      cgtatggtg tgctgggga caaagactg tctccaata 
      atatcatgg aagtgtacc atactttgg gaatagaag 
      ccgcaagag aaaccatta tgtccgaaa ttaaaatgg 
      taatggaaa accatggta tgagcgtgg attatcgcc 
      acattatgt tactcgcgg cccaaatga cacacacgg 
      gggaagttc tgggcatca cacgatatg gtttatcca 
      aaatgaagc agtccatac taaacttgg cttcttttg 
      agaaaaccg ctgatcatt tgtttgatg ctgcttatt 
      acggccagg ttgacaaaa taaacggcg tttcagaaa 
      atattataa tgggaatgc ctgcaccta taggaaccg 
      gaattttca aactcctcc acaagccac accacatcg 
      aggaaaatc caccatcag aacctttac tatttgaaa 
      ctgcgattg ccaagtaaa atatgtatt tgagaaatt 
      gtacaataa aaattataa atcaag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY