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Gene information
General information
TC003717 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 13617744..13634437
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003717
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02717

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    procephalic head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003717 RA
      MDLPYRAEY AKSGRSNCR GCKSPIGQG TVRLAVMVQ 
      SPFHDGMMP NWHHLHCFF NKQRPKSED DIYHFETLR 
      LEDQEIIRE KLSNMGSII VPEKKGKGK KRDATAIAQ 
      KNAALKDFK IEYAKSGRA ACRGCEQKI LKDEIRISK 
      KDFETDIGK KYGGQDMWH HVTCFAQLR AELGYFESA 
      DQLPGFKSL KKEDQAETK KALPAIKQE DVPDVKKVK 
      KEEDVVDGF TEEQSKLYK EQTELIFKH RDQLEKNLS 
      KEELAMLLQ HNDQEVPPG KDRVLDRLA DIMTFGALV 
      PCSVCKHGQ LVFEKFGYI CKGNLTEYT KCRTQVKEP 
      PRKPFKVPA ELKQYPFLK KYKYVPRTR VVKNAPPSY 
      SVKKEEGEE NAEPKVKRE APALYNMEF VILGQPDMG 
      KEELKRRIV KLGGKVVTK ISGTVMAVF ASKADVEKM 
      GARMKVVQE EKIHVVSED FIEEAPQHK GKIPELVIS 
      KSICDWGSD PTTRLPPER SSSKSKSGS IYTKSMPSK 
      VKLTVKGGS AVDPDSGLE HKAHVYTTE HNKYFAVLS 
      KTDIQTNKN SYYKLQLLE ADAGNKYWI FRSWGRIGT 
      TIGGNKLEK FYHLHDALL EFHKQYEEK TGNRWENRF 
      EFQKIPALF CPVDVDFGD VDEDQSKSM DITDSASTL 
      PLPIQNLIR KIFDVKAMQ KLMLEFELD TEKMPLGKL 
      SKKQISKAY AVLAELQQL VDTGGAEAM LVDASNRFY 
      TFIPHSFGV DNPPTLNDA ETIKKKIEM LDSLMELEI 
      AYNLMKSSG SEHTVDSYY KQLNTEIDV LKTDSEEYK 
      IIQEYVTNT HAATHGGYS LNIKNVFTI KRQGEDKRF 
      RPFKKLHNH KLLWHGSRT TNFAGILSK GCALRRRKL 
      PLLGTCSVK VSTLPTWCP SRPITVVLR FRIRLDCCC 
      CVMLRWEIL TRETKLSTW KSYQKGNTV PKVWAGLSL 
      TLKHPRPLT ELRCR
    CDS sequence
    • >TC003717 RA
      atggatctg ccataccgg gcagaatat gcgaaaagt 
      ggaaggtca aattgtcgt gggtgtaaa tcacccatt 
      ggacaaggt accgtgcga ctggcggtc atggtccag 
      tctcctttc cacgatgga atgatgcca aactggcat 
      catctccac tgttttttc aacaaacaa cgccccaaa 
      tcagaagac gacatttac cactttgaa actttacga 
      cttgaagac caggagatt attagagaa aagttgtca 
      aatatgggg tcaatcata gtacccgag aaaaaaggc 
      aaaggtaaa aagagagac gcaacagcc attgctcag 
      aaaaatgcc gctcttaaa gacttcaaa atagaatat 
      gccaagtca ggtagagct gcttgcaga ggctgtgaa 
      cagaaaata ttgaaagac gagataaga atttccaaa 
      aaagatttt gaaactgat attggtaag aaatatgga 
      ggacaggat atgtggcac cacgtgact tgctttgca 
      cagttaagg gccgaattg gggtatttt gagtccgca 
      gaccaactg cctggcttc aagtcgttg aaaaaagag 
      gatcaggct gaaactaaa aaagcattg cctgccatc 
      aagcaagaa gacgttcca gatgttaaa aaagttaaa 
      aaagaagag gacgttgtt gatggtttt acagaagaa 
      cagtcaaaa ttgtacaag gaacaaact gaacttatc 
      tttaaacac agagatcag ttggagaag aatctcagt 
      aaggaagaa cttgctatg ttgttgcaa cataacgat 
      caagaagtt ccaccgggc aaagacaga gttttggat 
      cgtctggct gacattatg acttttggt gccttggtt 
      ccatgctcc gtttgtaag cacggacag ttagttttt 
      gagaaattt ggttacatt tgtaaggga aacttgact 
      gaatataca aagtgtcgc acccaagtt aaggagccg 
      cctcgtaag ccgtttaaa gtgcccgcg gaattgaaa 
      caatacccg tttttgaaa aagtataaa tacgtgccc 
      agaacccgt gtggtgaaa aatgcgcct ccctcctac 
      tctgtgaaa aaagaagag ggcgaggaa aatgccgaa 
      ccaaaagtg aaaagagag gccccagcg ctgtataat 
      atggagttc gtcattttg ggacaacct gatatggga 
      aaagaagaa ctaaaaaga cggattgtt aagttgggg 
      ggtaaagtc gttaccaaa atcagtgga actgtgatg 
      gctgtgttt gcttcaaag gctgatgtt gagaaaatg 
      ggagctagg atgaaagtg gttcaagag gaaaaaatc 
      catgtagtt tctgaggat ttcatcgaa gaagcgccg 
      caacataag gggaaaatt ccagaactc gttatttcg 
      aaaagtatc tgcgactgg gggtcagat cccaccact 
      cgtcttcct cctgaacga tccagcagc aaatcgaaa 
      tctggcagt atctacacc aagtcaatg ccaagcaaa 
      gtcaaactg accgtgaaa ggtggttca gctgtggat 
      ccagactcc ggtctggag cacaaagct cacgtatac 
      acgaccgaa cacaacaaa tactttgct gtattgagc 
      aaaacggat atacaaacc aacaagaac agttactac 
      aagctccaa ttactggag gccgatgcc ggtaacaaa 
      tactggatt ttccgatca tggggtcgc attggtact 
      accatcggt ggcaataaa ttggaaaag ttctatcat 
      ctccatgat gcattgctc gaattccat aaacaatat 
      gaggaaaaa acaggaaac aggtgggag aatcggttt 
      gaattccag aaaatcccc gcacttttt tgccctgtg 
      gatgtcgat tttggggat gttgatgag gatcagagt 
      aagagcatg gatataacg gattcggcg tcgacgttg 
      ccacttccc atccaaaac ttgattcgg aagattttt 
      gatgtgaaa gcgatgcag aaactcatg ctggagttt 
      gagttagat actgagaaa atgccgcta gggaagttg 
      agcaaaaag cagatttcg aaggcctat gccgtcctg 
      gctgagctg caacagctc gtggacacg ggcggagct 
      gaggccatg ttggtagat gcctcgaat cggttctac 
      actttcatt ccacactcg ttcggagtg gacaatcct 
      ccgacactc aatgatgcg gaaacaatt aagaagaaa 
      atcgagatg ttggatagt ttgatggag ctggaaata 
      gcatacaac ttgatgaaa tcgagcggt tcggagcac 
      accgttgac agttactac aaacaattg aatacagaa 
      attgatgtt ttgaaaact gatagtgaa gaatacaaa 
      atcatacaa gagtatgtg acaaatacg catgccgct 
      actcatggc ggttattct ctcaacatc aaaaacgtc 
      ttcactatt aagcgccaa ggggaggat aagagattc 
      agaccgttt aagaaacta cacaatcac aagttattg 
      tggcacggt tcacgtacc actaacttt gcggggatt 
      ttgtccaag ggttgcgca ttgcgccgc cggaagctc 
      ccgttactg ggtacatgt tcggtaaag gtatctact 
      ttgccgaca tggtgtcca agtcggcca attactgtt 
      gtacttcgg ttcagaatc cgactggat tgttgctgc 
      tgtgtgatg ttgcgctgg gaaatactt acgagagaa 
      accaagctg agtacgtgg aaaagttac caaaaggga 
      aacacagta ccaaaggtt tgggcagga ctgagcctg 
      accctaaag catccaaga cccttgacg gaattgagg 
      tgccgctag
    mRNA sequence
    • >TC003717 RA
      aaatatttg tcaattgtt cattttgaa aatttcccg 
      gaagaaatc attcaattt tctaattta aaaatttgg 
      cgggttgct caggtcgcg caacgtaac agatgtagt 
      tttactaaa taagaacct tgtttttag aaattttta 
      ggacttgtg gttggtcac atgttttgt tattgcgca 
      ttttcccgg tattttcag caccgctgt tttaggttg 
      tgtgtttga ttgttgtgt aaaagtggt tgtttacgc 
      aaaataagt aaaaatgga tctgccata ccgggcaga 
      atatgcgaa aagtggaag gtcaaattg tcgtgggtg 
      taaatcacc cattggaca aggtaccgt gcgactggc 
      ggtcatggt ccagtctcc tttccacga tggaatgat 
      gccaaactg gcatcatct ccactgttt tttcaacaa 
      acaacgccc caaatcaga agacgacat ttaccactt 
      tgaaacttt acgacttga agaccagga gattattag 
      agaaaagtt gtcaaatat ggggtcaat catagtacc 
      cgagaaaaa aggcaaagg taaaaagag agacgcaac 
      agccattgc tcagaaaaa tgccgctct taaagactt 
      caaaataga atatgccaa gtcaggtag agctgcttg 
      cagaggctg tgaacagaa aatattgaa agacgagat 
      aagaatttc caaaaaaga ttttgaaac tgatattgg 
      taagaaata tggaggaca ggatatgtg gcaccacgt 
      gacttgctt tgcacagtt aagggccga attggggta 
      ttttgagtc cgcagacca actgcctgg cttcaagtc 
      gttgaaaaa agaggatca ggctgaaac taaaaaagc 
      attgcctgc catcaagca agaagacgt tccagatgt 
      taaaaaagt taaaaaaga agaggacgt tgttgatgg 
      ttttacaga agaacagtc aaaattgta caaggaaca 
      aactgaact tatctttaa acacagaga tcagttgga 
      gaagaatct cagtaagga agaacttgc tatgttgtt 
      gcaacataa cgatcaaga agttccacc gggcaaaga 
      cagagtttt ggatcgtct ggctgacat tatgacttt 
      tggtgcctt ggttccatg ctccgtttg taagcacgg 
      acagttagt ttttgagaa atttggtta catttgtaa 
      gggaaactt gactgaata tacaaagtg tcgcaccca 
      agttaagga gccgcctcg taagccgtt taaagtgcc 
      cgcggaatt gaaacaata cccgttttt gaaaaagta 
      taaatacgt gcccagaac ccgtgtggt gaaaaatgc 
      gcctccctc ctactctgt gaaaaaaga agagggcga 
      ggaaaatgc cgaaccaaa agtgaaaag agaggcccc 
      agcgctgta taatatgga gttcgtcat tttgggaca 
      acctgatat gggaaaaga agaactaaa aagacggat 
      tgttaagtt ggggggtaa agtcgttac caaaatcag 
      tggaactgt gatggctgt gtttgcttc aaaggctga 
      tgttgagaa aatgggagc taggatgaa agtggttca 
      agaggaaaa aatccatgt agtttctga ggatttcat 
      cgaagaagc gccgcaaca taaggggaa aattccaga 
      actcgttat ttcgaaaag tatctgcga ctgggggtc 
      agatcccac cactcgtct tcctcctga acgatccag 
      cagcaaatc gaaatctgg cagtatcta caccaagtc 
      aatgccaag caaagtcaa actgaccgt gaaaggtgg 
      ttcagctgt ggatccaga ctccggtct ggagcacaa 
      agctcacgt atacacgac cgaacacaa caaatactt 
      tgctgtatt gagcaaaac ggatataca aaccaacaa 
      gaacagtta ctacaagct ccaattact ggaggccga 
      tgccggtaa caaatactg gattttccg atcatgggg 
      tcgcattgg tactaccat cggtggcaa taaattgga 
      aaagttcta tcatctcca tgatgcatt gctcgaatt 
      ccataaaca atatgagga aaaaacagg aaacaggtg 
      ggagaatcg gtttgaatt ccagaaaat ccccgcact 
      tttttgccc tgtggatgt cgattttgg ggatgttga 
      tgaggatca gagtaagag catggatat aacggattc 
      ggcgtcgac gttgccact tcccatcca aaacttgat 
      tcggaagat ttttgatgt gaaagcgat gcagaaact 
      catgctgga gtttgagtt agatactga gaaaatgcc 
      gctagggaa gttgagcaa aaagcagat ttcgaaggc 
      ctatgccgt cctggctga gctgcaaca gctcgtgga 
      cacgggcgg agctgaggc catgttggt agatgcctc 
      gaatcggtt ctacacttt cattccaca ctcgttcgg 
      agtggacaa tcctccgac actcaatga tgcggaaac 
      aattaagaa gaaaatcga gatgttgga tagtttgat 
      ggagctgga aatagcata caacttgat gaaatcgag 
      cggttcgga gcacaccgt tgacagtta ctacaaaca 
      attgaatac agaaattga tgttttgaa aactgatag 
      tgaagaata caaaatcat acaagagta tgtgacaaa 
      tacgcatgc cgctactca tggcggtta ttctctcaa 
      catcaaaaa cgtcttcac tattaagcg ccaagggga 
      ggataagag attcagacc gtttaagaa actacacaa 
      tcacaagtt attgtggca cggttcacg taccactaa 
      ctttgcggg gattttgtc caagggttg cgcattgcg 
      ccgccggaa gctcccgtt actgggtac atgttcggt 
      aaaggtatc tactttgcc gacatggtg tccaagtcg 
      gccaattac tgttgtact tcggttcag aatccgact 
      ggattgttg ctgctgtgt gatgttgcg ctgggaaat 
      acttacgag agaaaccaa gctgagtac gtggaaaag 
      ttaccaaaa gggaaacac agtaccaaa ggtttgggc 
      aggactgag cctgaccct aaagcatcc aagaccctt 
      gacggaatt gaggtgccg ctagggaag ggcgttact 
      aatgataat atgcgctca gctttgctt tataacgag 
      tatattgtt tacgatgtg gcgcaggtc aacgttaag 
      tatttggtt gaagttgac ttcaagtat aaatactaa 
      aatgtgctt tccaatgtt tttaatgtt tacaagttt 
      gaaattacc aagtttaca ataaaaagt gttgcgatt 
      actacgaaa gatgtctgt attgattta cttccattt 
      tttcccgtt cctggtact aaaattgtt gatttatca 
      cccagactg ccgctgttg ttatcgtct gaagctttt 
      ccactgcct ccattacag aggtcttct ttacatgcc 
      acaaaatac cacctgtaa gtggcgtct tccacaatt 
      tcgttcata tgttctggc gtatcctca catcctcta 
      aacattgaa acaactttc ccttgaggt tcctaatac 
      ataaatatt gaattacgt cccctaact ggtgtaata 
      gcttactaa taactcttg ccgttttgt ggtacaata 
      ttttttgtt acaatttgt tggtgtcgt tttttcagg 
      cgtatcaat acatgtcat atcagtgtc ttgctcacc 
      cactgaaca cgtgtaaca ttttaaagt tacaacaga 
      gtggtaaaa ggaagcaca gagtatcaa aaacaacgg 
      taccttcat gacttaaaa tttccaaat actagaaat 
      ctttagaat gattaaaag ataaggcgt tcg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY