Status
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Gene information
General information
TC003717 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 13617744..13634437
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003717
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02717

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    procephalic head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003717 RA
      MDLPYRAEY AKSGRSNCR GCKSPIGQG TVRLAVMVQ 
SPFHDGMMP NWHHLHCFF NKQRPKSED DIYHFETLR 
LEDQEIIRE KLSNMGSII VPEKKGKGK KRDATAIAQ 
KNAALKDFK IEYAKSGRA ACRGCEQKI LKDEIRISK 
KDFETDIGK KYGGQDMWH HVTCFAQLR AELGYFESA 
DQLPGFKSL KKEDQAETK KALPAIKQE DVPDVKKVK 
KEEDVVDGF TEEQSKLYK EQTELIFKH RDQLEKNLS 
KEELAMLLQ HNDQEVPPG KDRVLDRLA DIMTFGALV 
PCSVCKHGQ LVFEKFGYI CKGNLTEYT KCRTQVKEP 
PRKPFKVPA ELKQYPFLK KYKYVPRTR VVKNAPPSY 
SVKKEEGEE NAEPKVKRE APALYNMEF VILGQPDMG 
KEELKRRIV KLGGKVVTK ISGTVMAVF ASKADVEKM 
GARMKVVQE EKIHVVSED FIEEAPQHK GKIPELVIS 
KSICDWGSD PTTRLPPER SSSKSKSGS IYTKSMPSK 
VKLTVKGGS AVDPDSGLE HKAHVYTTE HNKYFAVLS 
KTDIQTNKN SYYKLQLLE ADAGNKYWI FRSWGRIGT 
TIGGNKLEK FYHLHDALL EFHKQYEEK TGNRWENRF 
EFQKIPALF CPVDVDFGD VDEDQSKSM DITDSASTL 
PLPIQNLIR KIFDVKAMQ KLMLEFELD TEKMPLGKL 
SKKQISKAY AVLAELQQL VDTGGAEAM LVDASNRFY 
TFIPHSFGV DNPPTLNDA ETIKKKIEM LDSLMELEI 
AYNLMKSSG SEHTVDSYY KQLNTEIDV LKTDSEEYK 
IIQEYVTNT HAATHGGYS LNIKNVFTI KRQGEDKRF 
RPFKKLHNH KLLWHGSRT TNFAGILSK GCALRRRKL 
PLLGTCSVK VSTLPTWCP SRPITVVLR FRIRLDCCC 
CVMLRWEIL TRETKLSTW KSYQKGNTV PKVWAGLSL 
TLKHPRPLT ELRCR
    CDS sequence
    • >TC003717 RA
      atggatctg ccataccgg gcagaatat gcgaaaagt 
ggaaggtca aattgtcgt gggtgtaaa tcacccatt 
ggacaaggt accgtgcga ctggcggtc atggtccag 
tctcctttc cacgatgga atgatgcca aactggcat 
catctccac tgttttttc aacaaacaa cgccccaaa 
tcagaagac gacatttac cactttgaa actttacga 
cttgaagac caggagatt attagagaa aagttgtca 
aatatgggg tcaatcata gtacccgag aaaaaaggc 
aaaggtaaa aagagagac gcaacagcc attgctcag 
aaaaatgcc gctcttaaa gacttcaaa atagaatat 
gccaagtca ggtagagct gcttgcaga ggctgtgaa 
cagaaaata ttgaaagac gagataaga atttccaaa 
aaagatttt gaaactgat attggtaag aaatatgga 
ggacaggat atgtggcac cacgtgact tgctttgca 
cagttaagg gccgaattg gggtatttt gagtccgca 
gaccaactg cctggcttc aagtcgttg aaaaaagag 
gatcaggct gaaactaaa aaagcattg cctgccatc 
aagcaagaa gacgttcca gatgttaaa aaagttaaa 
aaagaagag gacgttgtt gatggtttt acagaagaa 
cagtcaaaa ttgtacaag gaacaaact gaacttatc 
tttaaacac agagatcag ttggagaag aatctcagt 
aaggaagaa cttgctatg ttgttgcaa cataacgat 
caagaagtt ccaccgggc aaagacaga gttttggat 
cgtctggct gacattatg acttttggt gccttggtt 
ccatgctcc gtttgtaag cacggacag ttagttttt 
gagaaattt ggttacatt tgtaaggga aacttgact 
gaatataca aagtgtcgc acccaagtt aaggagccg 
cctcgtaag ccgtttaaa gtgcccgcg gaattgaaa 
caatacccg tttttgaaa aagtataaa tacgtgccc 
agaacccgt gtggtgaaa aatgcgcct ccctcctac 
tctgtgaaa aaagaagag ggcgaggaa aatgccgaa 
ccaaaagtg aaaagagag gccccagcg ctgtataat 
atggagttc gtcattttg ggacaacct gatatggga 
aaagaagaa ctaaaaaga cggattgtt aagttgggg 
ggtaaagtc gttaccaaa atcagtgga actgtgatg 
gctgtgttt gcttcaaag gctgatgtt gagaaaatg 
ggagctagg atgaaagtg gttcaagag gaaaaaatc 
catgtagtt tctgaggat ttcatcgaa gaagcgccg 
caacataag gggaaaatt ccagaactc gttatttcg 
aaaagtatc tgcgactgg gggtcagat cccaccact 
cgtcttcct cctgaacga tccagcagc aaatcgaaa 
tctggcagt atctacacc aagtcaatg ccaagcaaa 
gtcaaactg accgtgaaa ggtggttca gctgtggat 
ccagactcc ggtctggag cacaaagct cacgtatac 
acgaccgaa cacaacaaa tactttgct gtattgagc 
aaaacggat atacaaacc aacaagaac agttactac 
aagctccaa ttactggag gccgatgcc ggtaacaaa 
tactggatt ttccgatca tggggtcgc attggtact 
accatcggt ggcaataaa ttggaaaag ttctatcat 
ctccatgat gcattgctc gaattccat aaacaatat 
gaggaaaaa acaggaaac aggtgggag aatcggttt 
gaattccag aaaatcccc gcacttttt tgccctgtg 
gatgtcgat tttggggat gttgatgag gatcagagt 
aagagcatg gatataacg gattcggcg tcgacgttg 
ccacttccc atccaaaac ttgattcgg aagattttt 
gatgtgaaa gcgatgcag aaactcatg ctggagttt 
gagttagat actgagaaa atgccgcta gggaagttg 
agcaaaaag cagatttcg aaggcctat gccgtcctg 
gctgagctg caacagctc gtggacacg ggcggagct 
gaggccatg ttggtagat gcctcgaat cggttctac 
actttcatt ccacactcg ttcggagtg gacaatcct 
ccgacactc aatgatgcg gaaacaatt aagaagaaa 
atcgagatg ttggatagt ttgatggag ctggaaata 
gcatacaac ttgatgaaa tcgagcggt tcggagcac 
accgttgac agttactac aaacaattg aatacagaa 
attgatgtt ttgaaaact gatagtgaa gaatacaaa 
atcatacaa gagtatgtg acaaatacg catgccgct 
actcatggc ggttattct ctcaacatc aaaaacgtc 
ttcactatt aagcgccaa ggggaggat aagagattc 
agaccgttt aagaaacta cacaatcac aagttattg 
tggcacggt tcacgtacc actaacttt gcggggatt 
ttgtccaag ggttgcgca ttgcgccgc cggaagctc 
ccgttactg ggtacatgt tcggtaaag gtatctact 
ttgccgaca tggtgtcca agtcggcca attactgtt 
gtacttcgg ttcagaatc cgactggat tgttgctgc 
tgtgtgatg ttgcgctgg gaaatactt acgagagaa 
accaagctg agtacgtgg aaaagttac caaaaggga 
aacacagta ccaaaggtt tgggcagga ctgagcctg 
accctaaag catccaaga cccttgacg gaattgagg 
tgccgctag
    mRNA sequence
    • >TC003717 RA
      aaatatttg tcaattgtt cattttgaa aatttcccg 
gaagaaatc attcaattt tctaattta aaaatttgg 
cgggttgct caggtcgcg caacgtaac agatgtagt 
tttactaaa taagaacct tgtttttag aaattttta 
ggacttgtg gttggtcac atgttttgt tattgcgca 
ttttcccgg tattttcag caccgctgt tttaggttg 
tgtgtttga ttgttgtgt aaaagtggt tgtttacgc 
aaaataagt aaaaatgga tctgccata ccgggcaga 
atatgcgaa aagtggaag gtcaaattg tcgtgggtg 
taaatcacc cattggaca aggtaccgt gcgactggc 
ggtcatggt ccagtctcc tttccacga tggaatgat 
gccaaactg gcatcatct ccactgttt tttcaacaa 
acaacgccc caaatcaga agacgacat ttaccactt 
tgaaacttt acgacttga agaccagga gattattag 
agaaaagtt gtcaaatat ggggtcaat catagtacc 
cgagaaaaa aggcaaagg taaaaagag agacgcaac 
agccattgc tcagaaaaa tgccgctct taaagactt 
caaaataga atatgccaa gtcaggtag agctgcttg 
cagaggctg tgaacagaa aatattgaa agacgagat 
aagaatttc caaaaaaga ttttgaaac tgatattgg 
taagaaata tggaggaca ggatatgtg gcaccacgt 
gacttgctt tgcacagtt aagggccga attggggta 
ttttgagtc cgcagacca actgcctgg cttcaagtc 
gttgaaaaa agaggatca ggctgaaac taaaaaagc 
attgcctgc catcaagca agaagacgt tccagatgt 
taaaaaagt taaaaaaga agaggacgt tgttgatgg 
ttttacaga agaacagtc aaaattgta caaggaaca 
aactgaact tatctttaa acacagaga tcagttgga 
gaagaatct cagtaagga agaacttgc tatgttgtt 
gcaacataa cgatcaaga agttccacc gggcaaaga 
cagagtttt ggatcgtct ggctgacat tatgacttt 
tggtgcctt ggttccatg ctccgtttg taagcacgg 
acagttagt ttttgagaa atttggtta catttgtaa 
gggaaactt gactgaata tacaaagtg tcgcaccca 
agttaagga gccgcctcg taagccgtt taaagtgcc 
cgcggaatt gaaacaata cccgttttt gaaaaagta 
taaatacgt gcccagaac ccgtgtggt gaaaaatgc 
gcctccctc ctactctgt gaaaaaaga agagggcga 
ggaaaatgc cgaaccaaa agtgaaaag agaggcccc 
agcgctgta taatatgga gttcgtcat tttgggaca 
acctgatat gggaaaaga agaactaaa aagacggat 
tgttaagtt ggggggtaa agtcgttac caaaatcag 
tggaactgt gatggctgt gtttgcttc aaaggctga 
tgttgagaa aatgggagc taggatgaa agtggttca 
agaggaaaa aatccatgt agtttctga ggatttcat 
cgaagaagc gccgcaaca taaggggaa aattccaga 
actcgttat ttcgaaaag tatctgcga ctgggggtc 
agatcccac cactcgtct tcctcctga acgatccag 
cagcaaatc gaaatctgg cagtatcta caccaagtc 
aatgccaag caaagtcaa actgaccgt gaaaggtgg 
ttcagctgt ggatccaga ctccggtct ggagcacaa 
agctcacgt atacacgac cgaacacaa caaatactt 
tgctgtatt gagcaaaac ggatataca aaccaacaa 
gaacagtta ctacaagct ccaattact ggaggccga 
tgccggtaa caaatactg gattttccg atcatgggg 
tcgcattgg tactaccat cggtggcaa taaattgga 
aaagttcta tcatctcca tgatgcatt gctcgaatt 
ccataaaca atatgagga aaaaacagg aaacaggtg 
ggagaatcg gtttgaatt ccagaaaat ccccgcact 
tttttgccc tgtggatgt cgattttgg ggatgttga 
tgaggatca gagtaagag catggatat aacggattc 
ggcgtcgac gttgccact tcccatcca aaacttgat 
tcggaagat ttttgatgt gaaagcgat gcagaaact 
catgctgga gtttgagtt agatactga gaaaatgcc 
gctagggaa gttgagcaa aaagcagat ttcgaaggc 
ctatgccgt cctggctga gctgcaaca gctcgtgga 
cacgggcgg agctgaggc catgttggt agatgcctc 
gaatcggtt ctacacttt cattccaca ctcgttcgg 
agtggacaa tcctccgac actcaatga tgcggaaac 
aattaagaa gaaaatcga gatgttgga tagtttgat 
ggagctgga aatagcata caacttgat gaaatcgag 
cggttcgga gcacaccgt tgacagtta ctacaaaca 
attgaatac agaaattga tgttttgaa aactgatag 
tgaagaata caaaatcat acaagagta tgtgacaaa 
tacgcatgc cgctactca tggcggtta ttctctcaa 
catcaaaaa cgtcttcac tattaagcg ccaagggga 
ggataagag attcagacc gtttaagaa actacacaa 
tcacaagtt attgtggca cggttcacg taccactaa 
ctttgcggg gattttgtc caagggttg cgcattgcg 
ccgccggaa gctcccgtt actgggtac atgttcggt 
aaaggtatc tactttgcc gacatggtg tccaagtcg 
gccaattac tgttgtact tcggttcag aatccgact 
ggattgttg ctgctgtgt gatgttgcg ctgggaaat 
acttacgag agaaaccaa gctgagtac gtggaaaag 
ttaccaaaa gggaaacac agtaccaaa ggtttgggc 
aggactgag cctgaccct aaagcatcc aagaccctt 
gacggaatt gaggtgccg ctagggaag ggcgttact 
aatgataat atgcgctca gctttgctt tataacgag 
tatattgtt tacgatgtg gcgcaggtc aacgttaag 
tatttggtt gaagttgac ttcaagtat aaatactaa 
aatgtgctt tccaatgtt tttaatgtt tacaagttt 
gaaattacc aagtttaca ataaaaagt gttgcgatt 
actacgaaa gatgtctgt attgattta cttccattt 
tttcccgtt cctggtact aaaattgtt gatttatca 
cccagactg ccgctgttg ttatcgtct gaagctttt 
ccactgcct ccattacag aggtcttct ttacatgcc 
acaaaatac cacctgtaa gtggcgtct tccacaatt 
tcgttcata tgttctggc gtatcctca catcctcta 
aacattgaa acaactttc ccttgaggt tcctaatac 
ataaatatt gaattacgt cccctaact ggtgtaata 
gcttactaa taactcttg ccgttttgt ggtacaata 
ttttttgtt acaatttgt tggtgtcgt tttttcagg 
cgtatcaat acatgtcat atcagtgtc ttgctcacc 
cactgaaca cgtgtaaca ttttaaagt tacaacaga 
gtggtaaaa ggaagcaca gagtatcaa aaacaacgg 
taccttcat gacttaaaa tttccaaat actagaaat 
ctttagaat gattaaaag ataaggcgt tcg

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY