General information | |||
TC003717 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 13617744..13634437 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC003717 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02717 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
antenna not present - Penetrance (percentage of animals/eggs on slide): less than 30%
procephalic head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC003717 RA
MDLPYRAEY AKSGRSNCR GCKSPIGQG TVRLAVMVQ SPFHDGMMP NWHHLHCFF NKQRPKSED DIYHFETLR LEDQEIIRE KLSNMGSII VPEKKGKGK KRDATAIAQ KNAALKDFK IEYAKSGRA ACRGCEQKI LKDEIRISK KDFETDIGK KYGGQDMWH HVTCFAQLR AELGYFESA DQLPGFKSL KKEDQAETK KALPAIKQE DVPDVKKVK KEEDVVDGF TEEQSKLYK EQTELIFKH RDQLEKNLS KEELAMLLQ HNDQEVPPG KDRVLDRLA DIMTFGALV PCSVCKHGQ LVFEKFGYI CKGNLTEYT KCRTQVKEP PRKPFKVPA ELKQYPFLK KYKYVPRTR VVKNAPPSY SVKKEEGEE NAEPKVKRE APALYNMEF VILGQPDMG KEELKRRIV KLGGKVVTK ISGTVMAVF ASKADVEKM GARMKVVQE EKIHVVSED FIEEAPQHK GKIPELVIS KSICDWGSD PTTRLPPER SSSKSKSGS IYTKSMPSK VKLTVKGGS AVDPDSGLE HKAHVYTTE HNKYFAVLS KTDIQTNKN SYYKLQLLE ADAGNKYWI FRSWGRIGT TIGGNKLEK FYHLHDALL EFHKQYEEK TGNRWENRF EFQKIPALF CPVDVDFGD VDEDQSKSM DITDSASTL PLPIQNLIR KIFDVKAMQ KLMLEFELD TEKMPLGKL SKKQISKAY AVLAELQQL VDTGGAEAM LVDASNRFY TFIPHSFGV DNPPTLNDA ETIKKKIEM LDSLMELEI AYNLMKSSG SEHTVDSYY KQLNTEIDV LKTDSEEYK IIQEYVTNT HAATHGGYS LNIKNVFTI KRQGEDKRF RPFKKLHNH KLLWHGSRT TNFAGILSK GCALRRRKL PLLGTCSVK VSTLPTWCP SRPITVVLR FRIRLDCCC CVMLRWEIL TRETKLSTW KSYQKGNTV PKVWAGLSL TLKHPRPLT ELRCR
- >TC003717 RA
atggatctg ccataccgg gcagaatat gcgaaaagt ggaaggtca aattgtcgt gggtgtaaa tcacccatt ggacaaggt accgtgcga ctggcggtc atggtccag tctcctttc cacgatgga atgatgcca aactggcat catctccac tgttttttc aacaaacaa cgccccaaa tcagaagac gacatttac cactttgaa actttacga cttgaagac caggagatt attagagaa aagttgtca aatatgggg tcaatcata gtacccgag aaaaaaggc aaaggtaaa aagagagac gcaacagcc attgctcag aaaaatgcc gctcttaaa gacttcaaa atagaatat gccaagtca ggtagagct gcttgcaga ggctgtgaa cagaaaata ttgaaagac gagataaga atttccaaa aaagatttt gaaactgat attggtaag aaatatgga ggacaggat atgtggcac cacgtgact tgctttgca cagttaagg gccgaattg gggtatttt gagtccgca gaccaactg cctggcttc aagtcgttg aaaaaagag gatcaggct gaaactaaa aaagcattg cctgccatc aagcaagaa gacgttcca gatgttaaa aaagttaaa aaagaagag gacgttgtt gatggtttt acagaagaa cagtcaaaa ttgtacaag gaacaaact gaacttatc tttaaacac agagatcag ttggagaag aatctcagt aaggaagaa cttgctatg ttgttgcaa cataacgat caagaagtt ccaccgggc aaagacaga gttttggat cgtctggct gacattatg acttttggt gccttggtt ccatgctcc gtttgtaag cacggacag ttagttttt gagaaattt ggttacatt tgtaaggga aacttgact gaatataca aagtgtcgc acccaagtt aaggagccg cctcgtaag ccgtttaaa gtgcccgcg gaattgaaa caatacccg tttttgaaa aagtataaa tacgtgccc agaacccgt gtggtgaaa aatgcgcct ccctcctac tctgtgaaa aaagaagag ggcgaggaa aatgccgaa ccaaaagtg aaaagagag gccccagcg ctgtataat atggagttc gtcattttg ggacaacct gatatggga aaagaagaa ctaaaaaga cggattgtt aagttgggg ggtaaagtc gttaccaaa atcagtgga actgtgatg gctgtgttt gcttcaaag gctgatgtt gagaaaatg ggagctagg atgaaagtg gttcaagag gaaaaaatc catgtagtt tctgaggat ttcatcgaa gaagcgccg caacataag gggaaaatt ccagaactc gttatttcg aaaagtatc tgcgactgg gggtcagat cccaccact cgtcttcct cctgaacga tccagcagc aaatcgaaa tctggcagt atctacacc aagtcaatg ccaagcaaa gtcaaactg accgtgaaa ggtggttca gctgtggat ccagactcc ggtctggag cacaaagct cacgtatac acgaccgaa cacaacaaa tactttgct gtattgagc aaaacggat atacaaacc aacaagaac agttactac aagctccaa ttactggag gccgatgcc ggtaacaaa tactggatt ttccgatca tggggtcgc attggtact accatcggt ggcaataaa ttggaaaag ttctatcat ctccatgat gcattgctc gaattccat aaacaatat gaggaaaaa acaggaaac aggtgggag aatcggttt gaattccag aaaatcccc gcacttttt tgccctgtg gatgtcgat tttggggat gttgatgag gatcagagt aagagcatg gatataacg gattcggcg tcgacgttg ccacttccc atccaaaac ttgattcgg aagattttt gatgtgaaa gcgatgcag aaactcatg ctggagttt gagttagat actgagaaa atgccgcta gggaagttg agcaaaaag cagatttcg aaggcctat gccgtcctg gctgagctg caacagctc gtggacacg ggcggagct gaggccatg ttggtagat gcctcgaat cggttctac actttcatt ccacactcg ttcggagtg gacaatcct ccgacactc aatgatgcg gaaacaatt aagaagaaa atcgagatg ttggatagt ttgatggag ctggaaata gcatacaac ttgatgaaa tcgagcggt tcggagcac accgttgac agttactac aaacaattg aatacagaa attgatgtt ttgaaaact gatagtgaa gaatacaaa atcatacaa gagtatgtg acaaatacg catgccgct actcatggc ggttattct ctcaacatc aaaaacgtc ttcactatt aagcgccaa ggggaggat aagagattc agaccgttt aagaaacta cacaatcac aagttattg tggcacggt tcacgtacc actaacttt gcggggatt ttgtccaag ggttgcgca ttgcgccgc cggaagctc ccgttactg ggtacatgt tcggtaaag gtatctact ttgccgaca tggtgtcca agtcggcca attactgtt gtacttcgg ttcagaatc cgactggat tgttgctgc tgtgtgatg ttgcgctgg gaaatactt acgagagaa accaagctg agtacgtgg aaaagttac caaaaggga aacacagta ccaaaggtt tgggcagga ctgagcctg accctaaag catccaaga cccttgacg gaattgagg tgccgctag
- >TC003717 RA
aaatatttg tcaattgtt cattttgaa aatttcccg gaagaaatc attcaattt tctaattta aaaatttgg cgggttgct caggtcgcg caacgtaac agatgtagt tttactaaa taagaacct tgtttttag aaattttta ggacttgtg gttggtcac atgttttgt tattgcgca ttttcccgg tattttcag caccgctgt tttaggttg tgtgtttga ttgttgtgt aaaagtggt tgtttacgc aaaataagt aaaaatgga tctgccata ccgggcaga atatgcgaa aagtggaag gtcaaattg tcgtgggtg taaatcacc cattggaca aggtaccgt gcgactggc ggtcatggt ccagtctcc tttccacga tggaatgat gccaaactg gcatcatct ccactgttt tttcaacaa acaacgccc caaatcaga agacgacat ttaccactt tgaaacttt acgacttga agaccagga gattattag agaaaagtt gtcaaatat ggggtcaat catagtacc cgagaaaaa aggcaaagg taaaaagag agacgcaac agccattgc tcagaaaaa tgccgctct taaagactt caaaataga atatgccaa gtcaggtag agctgcttg cagaggctg tgaacagaa aatattgaa agacgagat aagaatttc caaaaaaga ttttgaaac tgatattgg taagaaata tggaggaca ggatatgtg gcaccacgt gacttgctt tgcacagtt aagggccga attggggta ttttgagtc cgcagacca actgcctgg cttcaagtc gttgaaaaa agaggatca ggctgaaac taaaaaagc attgcctgc catcaagca agaagacgt tccagatgt taaaaaagt taaaaaaga agaggacgt tgttgatgg ttttacaga agaacagtc aaaattgta caaggaaca aactgaact tatctttaa acacagaga tcagttgga gaagaatct cagtaagga agaacttgc tatgttgtt gcaacataa cgatcaaga agttccacc gggcaaaga cagagtttt ggatcgtct ggctgacat tatgacttt tggtgcctt ggttccatg ctccgtttg taagcacgg acagttagt ttttgagaa atttggtta catttgtaa gggaaactt gactgaata tacaaagtg tcgcaccca agttaagga gccgcctcg taagccgtt taaagtgcc cgcggaatt gaaacaata cccgttttt gaaaaagta taaatacgt gcccagaac ccgtgtggt gaaaaatgc gcctccctc ctactctgt gaaaaaaga agagggcga ggaaaatgc cgaaccaaa agtgaaaag agaggcccc agcgctgta taatatgga gttcgtcat tttgggaca acctgatat gggaaaaga agaactaaa aagacggat tgttaagtt ggggggtaa agtcgttac caaaatcag tggaactgt gatggctgt gtttgcttc aaaggctga tgttgagaa aatgggagc taggatgaa agtggttca agaggaaaa aatccatgt agtttctga ggatttcat cgaagaagc gccgcaaca taaggggaa aattccaga actcgttat ttcgaaaag tatctgcga ctgggggtc agatcccac cactcgtct tcctcctga acgatccag cagcaaatc gaaatctgg cagtatcta caccaagtc aatgccaag caaagtcaa actgaccgt gaaaggtgg ttcagctgt ggatccaga ctccggtct ggagcacaa agctcacgt atacacgac cgaacacaa caaatactt tgctgtatt gagcaaaac ggatataca aaccaacaa gaacagtta ctacaagct ccaattact ggaggccga tgccggtaa caaatactg gattttccg atcatgggg tcgcattgg tactaccat cggtggcaa taaattgga aaagttcta tcatctcca tgatgcatt gctcgaatt ccataaaca atatgagga aaaaacagg aaacaggtg ggagaatcg gtttgaatt ccagaaaat ccccgcact tttttgccc tgtggatgt cgattttgg ggatgttga tgaggatca gagtaagag catggatat aacggattc ggcgtcgac gttgccact tcccatcca aaacttgat tcggaagat ttttgatgt gaaagcgat gcagaaact catgctgga gtttgagtt agatactga gaaaatgcc gctagggaa gttgagcaa aaagcagat ttcgaaggc ctatgccgt cctggctga gctgcaaca gctcgtgga cacgggcgg agctgaggc catgttggt agatgcctc gaatcggtt ctacacttt cattccaca ctcgttcgg agtggacaa tcctccgac actcaatga tgcggaaac aattaagaa gaaaatcga gatgttgga tagtttgat ggagctgga aatagcata caacttgat gaaatcgag cggttcgga gcacaccgt tgacagtta ctacaaaca attgaatac agaaattga tgttttgaa aactgatag tgaagaata caaaatcat acaagagta tgtgacaaa tacgcatgc cgctactca tggcggtta ttctctcaa catcaaaaa cgtcttcac tattaagcg ccaagggga ggataagag attcagacc gtttaagaa actacacaa tcacaagtt attgtggca cggttcacg taccactaa ctttgcggg gattttgtc caagggttg cgcattgcg ccgccggaa gctcccgtt actgggtac atgttcggt aaaggtatc tactttgcc gacatggtg tccaagtcg gccaattac tgttgtact tcggttcag aatccgact ggattgttg ctgctgtgt gatgttgcg ctgggaaat acttacgag agaaaccaa gctgagtac gtggaaaag ttaccaaaa gggaaacac agtaccaaa ggtttgggc aggactgag cctgaccct aaagcatcc aagaccctt gacggaatt gaggtgccg ctagggaag ggcgttact aatgataat atgcgctca gctttgctt tataacgag tatattgtt tacgatgtg gcgcaggtc aacgttaag tatttggtt gaagttgac ttcaagtat aaatactaa aatgtgctt tccaatgtt tttaatgtt tacaagttt gaaattacc aagtttaca ataaaaagt gttgcgatt actacgaaa gatgtctgt attgattta cttccattt tttcccgtt cctggtact aaaattgtt gatttatca cccagactg ccgctgttg ttatcgtct gaagctttt ccactgcct ccattacag aggtcttct ttacatgcc acaaaatac cacctgtaa gtggcgtct tccacaatt tcgttcata tgttctggc gtatcctca catcctcta aacattgaa acaactttc ccttgaggt tcctaatac ataaatatt gaattacgt cccctaact ggtgtaata gcttactaa taactcttg ccgttttgt ggtacaata ttttttgtt acaatttgt tggtgtcgt tttttcagg cgtatcaat acatgtcat atcagtgtc ttgctcacc cactgaaca cgtgtaaca ttttaaagt tacaacaga gtggtaaaa ggaagcaca gagtatcaa aaacaacgg taccttcat gacttaaaa tttccaaat actagaaat ctttagaat gattaaaag ataaggcgt tcg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
