Status
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Gene information
General information
TC003698 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 12845317..12853859
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003698
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02715

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003698 RA
      MQPFTTFMN VESTRSYID NLYSSDPEK CLNSIICIK 
      NSVIGSNRQ KESVIAQGI VPRLLQLLE DKNTKLAVR 
      IEAAVTIGS LAKGTTEHS EILINSGTV ELLLDILEE 
      DEPRLVDAC LCCLRTLSL LDSATAQNK FDVKRLQKL 
      LTFAGPGES LQRQSCVAS ILGSACKTP VEQNELCNV 
      GAPNLLAAL LSVQNSSVR IPVLTCLAA MCWNNQNVA 
      NAIVNTTYK DIKVPNHLA TLISRDKPI EMQLEAAKC 
      LTNLHRAGA IPAYDPIIT YRTLPCLVR LCQIEHSEP 
      HRAAAANTL AYLTEVDSD LQQVAAISN QLVSALVHL 
      LSCRSVVAR EAAFRAFAS LAANDEDIR KRIIDTKCL 
      MDSVLEGLD DENENIRLA AVRCLHSLS RSVQQLRTT 
      FQDYSVWRP LMALLTGCP STELLTAAS SALCNFLLE 
      FSPAKEPMI QQGVVQLLA TLTVRPEPS LRLNGVWAL 
      MNLAFRAEQ RVKSQILTA LGTDQIFRL LGDSDTRVL 
      MKTLGLLRN LVSPRTHTD TMMSLHGPQ VMQGVVLVL 
      EGPYSPEVK EQALCILGN IADGEKARE HIMSNEDVL 
      KKLIGYMTH PAPCLQQAS VFCIDNLSR LGEPGSVER 
      QNKLKEMGV VNILQQLLS TSNTMLYNR VKTALTQYN 
      DV
    CDS sequence
    • >TC003698 RA
      atgcagcca tttacgact ttcatgaac gtagaaagc 
      acccgctcc tacatcgac aacctgtac tcgtcagat 
      cctgagaaa tgcctaaac tcgataatt tgcatcaaa 
      aactccgtt attggctcg aaccgacaa aaagagagc 
      gtgattgct cagggtata gtgccgcgc cttttgcag 
      ctcctcgaa gataaaaac acgaaattg gctgtaagg 
      atcgaggct gcagtgacg atcgggtcg ctggccaag 
      ggaacgacc gagcatagc gagattctc atcaattct 
      ggcactgtt gagctgctt ttggacatc ctggaggag 
      gacgagccc cgcttagta gacgcgtgt ttgtgttgc 
      ttgcgtact ttgtctctg ctggactcg gccacggct 
      caaaataag ttcgatgtg aagcggttg cagaagttg 
      ttgactttc gctgggccg ggggagagt ctccagaga 
      cagtcttgt gtggcgagt attctaggg tcagcgtgt 
      aagacgcca gtggaacaa aacgagttg tgtaatgtg 
      ggggcgccg aacttgctc gcggcgctc ctgtcagtg 
      caaaatagt tcggtgagg atacccgtc ttgacgtgt 
      ttggcggca atgtgttgg aacaaccaa aatgtagca 
      aatgctatt gtcaatacg acttacaaa gatattaaa 
      gttcccaac catttggcg actttgatc agtagggat 
      aaacccata gagatgcag ttggaggcc gccaaatgt 
      ctcacgaat ttacacaga gctggggct atcccagcc 
      tacgatcct ataataact tacagaact ctgccgtgt 
      ctagtccga ctatgtcag attgaacac tcggaacca 
      cacagggca gcagcagca aacacttta gcttattta 
      acagaagtc gatagtgat ttacagcaa gtcgctgca 
      attagtaat caattagtc agtgcttta gtacatttg 
      ttgagttgt cgtagtgtt gtggcgaga gaagcggcc 
      ttcagagcg tttgcttct ttggcagcg aatgacgaa 
      gacataaga aaaaggatt atcgacaca aagtgtcta 
      atggatagt gtgttggaa ggtttagat gatgaaaat 
      gaaaatatt aggttagca gctgtacgt tgtcttcat 
      tcgttgagt aggtcagtg caacagttg cgtaccacc 
      ttccaagat tactccgtg tggcgcccc ctgatggcg 
      cttttaaca ggttgccct tcgacggag ttgctcaca 
      gccgcctcg tccgccctt tgcaacttc ctcctggaa 
      ttctccccc gccaaagag cccatgatc cagcaaggc 
      gtcgtgcag ttgttggca accctgacc gtccgtccc 
      gagccatcc cttcgtctg aacggcgtc tgggctcta 
      atgaatctc gcgtttcgg gcagagcaa cgagtcaaa 
      tcccaaatc ttaaccgcc ctcggcacc gaccagatc 
      ttccggttg ctgggcgac tcggacacg cgcgtcctc 
      atgaagacc ttggggctg ttgcgcaac ctggtgtcc 
      ccgcggacg cacaccgac accatgatg tcgctgcac 
      ggcccccag gtcatgcag ggcgtggtg ctggtgctg 
      gaggggccg tattccccc gaagtgaag gagcaggcg 
      ctgtgcatc ctgggcaac atcgccgac ggcgagaag 
      gccagggag cacataatg tcgaacgag gacgtactt 
      aagaaatta atcggctat atgacgcac ccagcgccc 
      tgtttgcag caagcgtcc gttttctgc atcgataat 
      ttgtcgagg ttgggagag ccgggctct gtggagagg 
      cagaataag ttaaaggag atgggagtt gttaatatc 
      ctccagcag ttgttgagt acttctaat acgatgctg 
      tacaatcga gttaaaacg gcgctcacg cagtataat 
      gatgtatga
    mRNA sequence
    • >TC003698 RA
      tccgttgcg taaacgccg taaacgtca agttgaatg 
      tttaaagta caattttaa aataaactc gaaattagt 
      tgcgtgtct gttgtgatt acccgaaga accttttta 
      tttatttta gttgtgacc gtagaatga tatttgaga 
      tgcagccat ttacgactt tcatgaacg tagaaagca 
      cccgctcct acatcgaca acctgtact cgtcagatc 
      ctgagaaat gcctaaact cgataattt gcatcaaaa 
      actccgtta ttggctcga accgacaaa aagagagcg 
      tgattgctc agggtatag tgccgcgcc ttttgcagc 
      tcctcgaag ataaaaaca cgaaattgg ctgtaagga 
      tcgaggctg cagtgacga tcgggtcgc tggccaagg 
      gaacgaccg agcatagcg agattctca tcaattctg 
      gcactgttg agctgcttt tggacatcc tggaggagg 
      acgagcccc gcttagtag acgcgtgtt tgtgttgct 
      tgcgtactt tgtctctgc tggactcgg ccacggctc 
      aaaataagt tcgatgtga agcggttgc agaagttgt 
      tgactttcg ctgggccgg gggagagtc tccagagac 
      agtcttgtg tggcgagta ttctagggt cagcgtgta 
      agacgccag tggaacaaa acgagttgt gtaatgtgg 
      gggcgccga acttgctcg cggcgctcc tgtcagtgc 
      aaaatagtt cggtgagga tacccgtct tgacgtgtt 
      tggcggcaa tgtgttgga acaaccaaa atgtagcaa 
      atgctattg tcaatacga cttacaaag atattaaag 
      ttcccaacc atttggcga ctttgatca gtagggata 
      aacccatag agatgcagt tggaggccg ccaaatgtc 
      tcacgaatt tacacagag ctggggcta tcccagcct 
      acgatccta taataactt acagaactc tgccgtgtc 
      tagtccgac tatgtcaga ttgaacact cggaaccac 
      acagggcag cagcagcaa acactttag cttatttaa 
      cagaagtcg atagtgatt tacagcaag tcgctgcaa 
      ttagtaatc aattagtca gtgctttag tacatttgt 
      tgagttgtc gtagtgttg tggcgagag aagcggcct 
      tcagagcgt ttgcttctt tggcagcga atgacgaag 
      acataagaa aaaggatta tcgacacaa agtgtctaa 
      tggatagtg tgttggaag gtttagatg atgaaaatg 
      aaaatatta ggttagcag ctgtacgtt gtcttcatt 
      cgttgagta ggtcagtgc aacagttgc gtaccacct 
      tccaagatt actccgtgt ggcgccccc tgatggcgc 
      ttttaacag gttgccctt cgacggagt tgctcacag 
      ccgcctcgt ccgcccttt gcaacttcc tcctggaat 
      tctcccccg ccaaagagc ccatgatcc agcaaggcg 
      tcgtgcagt tgttggcaa ccctgaccg tccgtcccg 
      agccatccc ttcgtctga acggcgtct gggctctaa 
      tgaatctcg cgtttcggg cagagcaac gagtcaaat 
      cccaaatct taaccgccc tcggcaccg accagatct 
      tccggttgc tgggcgact cggacacgc gcgtcctca 
      tgaagacct tggggctgt tgcgcaacc tggtgtccc 
      cgcggacgc acaccgaca ccatgatgt cgctgcacg 
      gcccccagg tcatgcagg gcgtggtgc tggtgctgg 
      aggggccgt attcccccg aagtgaagg agcaggcgc 
      tgtgcatcc tgggcaaca tcgccgacg gcgagaagg 
      ccagggagc acataatgt cgaacgagg acgtactta 
      agaaattaa tcggctata tgacgcacc cagcgccct 
      gtttgcagc aagcgtccg ttttctgca tcgataatt 
      tgtcgaggt tgggagagc cgggctctg tggagaggc 
      agaataagt taaaggaga tgggagttg ttaatatcc 
      tccagcagt tgttgagta cttctaata cgatgctgt 
      acaatcgag ttaaaacgg cgctcacgc agtataatg 
      atgtatgaa gtttattta ctgttttaa ggatacatg 
      atcccccta tctgatatt aatgtgatg tgtagactg 
      acaagtgta tattttgtt ttttattat tattttatt 
      tgtaattat tgtaggatg ctgattata tgatccaag 
      atctaatta ttatgtttt acatcctgg ttaaaagca 
      catgacact ttttattcg aggtcagtt aggtacaat 
      tggagaaga ggatgaaaa tgacaaata aaatattga 
      ctctgttag catcggttt atgaatttg tagataatt 
      gtataaact ttacgcttt tagtcaaca ctttggctt 
      ttgttatcg attttaatt tacagcatc attaacaat 
      aaaagatta tttatttc
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