| General information | |||
| TC003698 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 12845317..12853859 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC003698 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02715 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC003698 RA
MQPFTTFMN VESTRSYID NLYSSDPEK CLNSIICIK NSVIGSNRQ KESVIAQGI VPRLLQLLE DKNTKLAVR IEAAVTIGS LAKGTTEHS EILINSGTV ELLLDILEE DEPRLVDAC LCCLRTLSL LDSATAQNK FDVKRLQKL LTFAGPGES LQRQSCVAS ILGSACKTP VEQNELCNV GAPNLLAAL LSVQNSSVR IPVLTCLAA MCWNNQNVA NAIVNTTYK DIKVPNHLA TLISRDKPI EMQLEAAKC LTNLHRAGA IPAYDPIIT YRTLPCLVR LCQIEHSEP HRAAAANTL AYLTEVDSD LQQVAAISN QLVSALVHL LSCRSVVAR EAAFRAFAS LAANDEDIR KRIIDTKCL MDSVLEGLD DENENIRLA AVRCLHSLS RSVQQLRTT FQDYSVWRP LMALLTGCP STELLTAAS SALCNFLLE FSPAKEPMI QQGVVQLLA TLTVRPEPS LRLNGVWAL MNLAFRAEQ RVKSQILTA LGTDQIFRL LGDSDTRVL MKTLGLLRN LVSPRTHTD TMMSLHGPQ VMQGVVLVL EGPYSPEVK EQALCILGN IADGEKARE HIMSNEDVL KKLIGYMTH PAPCLQQAS VFCIDNLSR LGEPGSVER QNKLKEMGV VNILQQLLS TSNTMLYNR VKTALTQYN DV
- >TC003698 RA
atgcagcca tttacgact ttcatgaac gtagaaagc acccgctcc tacatcgac aacctgtac tcgtcagat cctgagaaa tgcctaaac tcgataatt tgcatcaaa aactccgtt attggctcg aaccgacaa aaagagagc gtgattgct cagggtata gtgccgcgc cttttgcag ctcctcgaa gataaaaac acgaaattg gctgtaagg atcgaggct gcagtgacg atcgggtcg ctggccaag ggaacgacc gagcatagc gagattctc atcaattct ggcactgtt gagctgctt ttggacatc ctggaggag gacgagccc cgcttagta gacgcgtgt ttgtgttgc ttgcgtact ttgtctctg ctggactcg gccacggct caaaataag ttcgatgtg aagcggttg cagaagttg ttgactttc gctgggccg ggggagagt ctccagaga cagtcttgt gtggcgagt attctaggg tcagcgtgt aagacgcca gtggaacaa aacgagttg tgtaatgtg ggggcgccg aacttgctc gcggcgctc ctgtcagtg caaaatagt tcggtgagg atacccgtc ttgacgtgt ttggcggca atgtgttgg aacaaccaa aatgtagca aatgctatt gtcaatacg acttacaaa gatattaaa gttcccaac catttggcg actttgatc agtagggat aaacccata gagatgcag ttggaggcc gccaaatgt ctcacgaat ttacacaga gctggggct atcccagcc tacgatcct ataataact tacagaact ctgccgtgt ctagtccga ctatgtcag attgaacac tcggaacca cacagggca gcagcagca aacacttta gcttattta acagaagtc gatagtgat ttacagcaa gtcgctgca attagtaat caattagtc agtgcttta gtacatttg ttgagttgt cgtagtgtt gtggcgaga gaagcggcc ttcagagcg tttgcttct ttggcagcg aatgacgaa gacataaga aaaaggatt atcgacaca aagtgtcta atggatagt gtgttggaa ggtttagat gatgaaaat gaaaatatt aggttagca gctgtacgt tgtcttcat tcgttgagt aggtcagtg caacagttg cgtaccacc ttccaagat tactccgtg tggcgcccc ctgatggcg cttttaaca ggttgccct tcgacggag ttgctcaca gccgcctcg tccgccctt tgcaacttc ctcctggaa ttctccccc gccaaagag cccatgatc cagcaaggc gtcgtgcag ttgttggca accctgacc gtccgtccc gagccatcc cttcgtctg aacggcgtc tgggctcta atgaatctc gcgtttcgg gcagagcaa cgagtcaaa tcccaaatc ttaaccgcc ctcggcacc gaccagatc ttccggttg ctgggcgac tcggacacg cgcgtcctc atgaagacc ttggggctg ttgcgcaac ctggtgtcc ccgcggacg cacaccgac accatgatg tcgctgcac ggcccccag gtcatgcag ggcgtggtg ctggtgctg gaggggccg tattccccc gaagtgaag gagcaggcg ctgtgcatc ctgggcaac atcgccgac ggcgagaag gccagggag cacataatg tcgaacgag gacgtactt aagaaatta atcggctat atgacgcac ccagcgccc tgtttgcag caagcgtcc gttttctgc atcgataat ttgtcgagg ttgggagag ccgggctct gtggagagg cagaataag ttaaaggag atgggagtt gttaatatc ctccagcag ttgttgagt acttctaat acgatgctg tacaatcga gttaaaacg gcgctcacg cagtataat gatgtatga
- >TC003698 RA
tccgttgcg taaacgccg taaacgtca agttgaatg tttaaagta caattttaa aataaactc gaaattagt tgcgtgtct gttgtgatt acccgaaga accttttta tttatttta gttgtgacc gtagaatga tatttgaga tgcagccat ttacgactt tcatgaacg tagaaagca cccgctcct acatcgaca acctgtact cgtcagatc ctgagaaat gcctaaact cgataattt gcatcaaaa actccgtta ttggctcga accgacaaa aagagagcg tgattgctc agggtatag tgccgcgcc ttttgcagc tcctcgaag ataaaaaca cgaaattgg ctgtaagga tcgaggctg cagtgacga tcgggtcgc tggccaagg gaacgaccg agcatagcg agattctca tcaattctg gcactgttg agctgcttt tggacatcc tggaggagg acgagcccc gcttagtag acgcgtgtt tgtgttgct tgcgtactt tgtctctgc tggactcgg ccacggctc aaaataagt tcgatgtga agcggttgc agaagttgt tgactttcg ctgggccgg gggagagtc tccagagac agtcttgtg tggcgagta ttctagggt cagcgtgta agacgccag tggaacaaa acgagttgt gtaatgtgg gggcgccga acttgctcg cggcgctcc tgtcagtgc aaaatagtt cggtgagga tacccgtct tgacgtgtt tggcggcaa tgtgttgga acaaccaaa atgtagcaa atgctattg tcaatacga cttacaaag atattaaag ttcccaacc atttggcga ctttgatca gtagggata aacccatag agatgcagt tggaggccg ccaaatgtc tcacgaatt tacacagag ctggggcta tcccagcct acgatccta taataactt acagaactc tgccgtgtc tagtccgac tatgtcaga ttgaacact cggaaccac acagggcag cagcagcaa acactttag cttatttaa cagaagtcg atagtgatt tacagcaag tcgctgcaa ttagtaatc aattagtca gtgctttag tacatttgt tgagttgtc gtagtgttg tggcgagag aagcggcct tcagagcgt ttgcttctt tggcagcga atgacgaag acataagaa aaaggatta tcgacacaa agtgtctaa tggatagtg tgttggaag gtttagatg atgaaaatg aaaatatta ggttagcag ctgtacgtt gtcttcatt cgttgagta ggtcagtgc aacagttgc gtaccacct tccaagatt actccgtgt ggcgccccc tgatggcgc ttttaacag gttgccctt cgacggagt tgctcacag ccgcctcgt ccgcccttt gcaacttcc tcctggaat tctcccccg ccaaagagc ccatgatcc agcaaggcg tcgtgcagt tgttggcaa ccctgaccg tccgtcccg agccatccc ttcgtctga acggcgtct gggctctaa tgaatctcg cgtttcggg cagagcaac gagtcaaat cccaaatct taaccgccc tcggcaccg accagatct tccggttgc tgggcgact cggacacgc gcgtcctca tgaagacct tggggctgt tgcgcaacc tggtgtccc cgcggacgc acaccgaca ccatgatgt cgctgcacg gcccccagg tcatgcagg gcgtggtgc tggtgctgg aggggccgt attcccccg aagtgaagg agcaggcgc tgtgcatcc tgggcaaca tcgccgacg gcgagaagg ccagggagc acataatgt cgaacgagg acgtactta agaaattaa tcggctata tgacgcacc cagcgccct gtttgcagc aagcgtccg ttttctgca tcgataatt tgtcgaggt tgggagagc cgggctctg tggagaggc agaataagt taaaggaga tgggagttg ttaatatcc tccagcagt tgttgagta cttctaata cgatgctgt acaatcgag ttaaaacgg cgctcacgc agtataatg atgtatgaa gtttattta ctgttttaa ggatacatg atcccccta tctgatatt aatgtgatg tgtagactg acaagtgta tattttgtt ttttattat tattttatt tgtaattat tgtaggatg ctgattata tgatccaag atctaatta ttatgtttt acatcctgg ttaaaagca catgacact ttttattcg aggtcagtt aggtacaat tggagaaga ggatgaaaa tgacaaata aaatattga ctctgttag catcggttt atgaatttg tagataatt gtataaact ttacgcttt tagtcaaca ctttggctt ttgttatcg attttaatt tacagcatc attaacaat aaaagatta tttatttc
