Status
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Gene information
General information
TC002741 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 12792560..12805953
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002741
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02712

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head appendages sometimes partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002741 RA
      MTRIRYLLQ AFRSRRRNR HLSRTDSVS FEAFCNEIP 
      PQKCPATKE SSLNGDFNS SPLKRERLE EDYVRFAPQ 
      NCESTTCSR PSLPAILLV LMLLAVFLV LPLVYLLHC 
      FGVIYTQER YEHPTLLEK IHHTHKSSE TKVPTALPP 
      PPKPDYGKC KLVQERDRF DCYPENGAN QQGCEARGC 
      CWIPAKKKP KMGVPLATP YCFYPSNYG TYNYVNVTQ 
      TAYGLEAFL KRGYATAYP GDVEIIKLS AKFETETRL 
      HIKISDPLK NRFEPPFPE VPIVDKAAM NLSYLFYID 
      STKPGFRVV RRSDNTIIF DALSLPNLI FSDQFLQLS 
      GKLPSNYIY GIGEHRTRL LLSTQWSRF TLFNHDAIP 
      SFEKNLYGS HPFYLIMEN STKSHGFYL QNSNAMDVI 
      LQPTPAITF RPIGGVLDF YFFLGPTPS DVISQYTDL 
      IGRPFMPPY WGLGFHLCR FGYKTLNRT KLVMQRNID 
      AGIPLDTQW NDLDYMKSS NDFTYDSVN FKGLPQFVK 
      DLHLKGMHY IPLIDAGVS GSEPPGSYP PFDEGLKMD 
      IFVKNSSGK IFIGKVWNN KTTVWPDFT HPTTVDYWT 
      MMLKSLHDI VPFDGAWID MNEPSNFLS GSFNGCPKT 
      SLDSPPYLP SVDGGALNY KTMCMSAKH YAGLHYNVH 
      NLFGFTEAI VTSFAMSDI RGRRPMVIS RSTFAGHGH 
      YAGHWSGDV VSDWLDMRY TIPQLLSFS LFGVPLMGA 
      DICGFNGNT TRSLCNRWT QLGAFYPFS RNHNTDDGI 
      DQDPVAMGP EVVMSARKA LSMRYKLLP YLYTLFWAA 
      HTRGDTVAR PLFFEFPTD LKTYDIDTQ FLWGPALMI 
      VPVLEENST EVTAYLPEG LWYDIYTKS PIAGQGQSV 
      NLSAPLDTI PVLLRGGYI LPTQAPEQT TTRSRLNRI 
      EIVAAGDEQ MNAFGEFYW DDGDSLNSY EEKQYTLID 
      FWMERGVLR SENIFWSGT ENPPNLGAV TIVGITDSV 
      EEVLVNNVA QAFRYDTIH KYLLIENLN VELQKPVVV 
      TWK
    CDS sequence
    • >TC002741 RA
      atgacgcga attcgttat ttgctgcaa gcattccgg 
      tcccggcgg cgtaaccgc cacttatcc cgcactgat 
      tccgtgtct ttcgaggcc ttctgtaac gaaatcccg 
      ccacagaag tgccccgca accaaagaa tcatcccta 
      aatggcgac ttcaactcc tcaccgctc aaaagagaa 
      cgcctggag gaagattac gtccgcttc gccccacag 
      aactgcgaa tccaccact tgctcccgt cctagtctg 
      ccggcaatc ctgctggtg ctcatgctt ctggctgtg 
      tttttagtc cttcccttg gtatatttg ttacattgt 
      tttggagtt atttacact caagaacgc tacgaacac 
      ccgacttta ctcgaaaaa atccaccac acgcacaaa 
      tccagcgaa accaaagtt cctacagct ctgccgccg 
      cccccgaaa ccggattat ggtaaatgc aaactggtc 
      caagagcgc gatcggttc gattgctat cctgagaat 
      ggggccaat cagcagggg tgcgaggcc aggggctgc 
      tgctggatc ccggcgaaa aagaagccg aaaatgggg 
      gtgcccctg gccacccct tactgtttc tatccctcc 
      aattacggg acctataat tacgtaaat gtgacgcag 
      acggcctac ggtctggag gcgtttttg aaaaggggg 
      tacgcaacg gcctatcct ggagatgtt gaaattatt 
      aaacttagt gccaagttt gagacggaa acgaggctg 
      catattaag atttcagat ccgctgaaa aacaggttt 
      gaaccccct tttcctgaa gttcctatc gtcgataaa 
      gcggccatg aatttgagt tacctgttt tatattgac 
      tcgactaag cccggtttt cgggtggta agacgctca 
      gataatacc ataatcttt gacgcatta agtttgcca 
      aatttaatt ttttctgat cagttttta caactgagc 
      ggtaagtta ccgtctaat tatatttac ggaattgga 
      gaacaccgc actcgttta ttgttatcg acccaatgg 
      tccagattc acgttattc aaccatgac gcaatacca 
      tcttttgaa aaaaattta tacggcagt catcctttt 
      tatctaatt atggaaaac tcgactaaa agccacggt 
      ttttacctc caaaactcc aacgctatg gatgttatt 
      ctccaaccc acccccgct ataacattc cgaccaatc 
      ggcggtgtc ctagacttc tattttttc cttggcccc 
      acccccagt gacgtaatc agccaatac acggatttg 
      attgggcgg ccgttcatg ccgccatat tgggggttg 
      ggctttcat ttatgtcgg ttcggttac aaaactttg 
      aaccgaact aaactcgtg atgcaacgt aacatcgat 
      gccgggatt ccattggac acacaatgg aacgatttg 
      gactacatg aaaagtagc aacgatttt acttacgat 
      tccgtcaat ttcaaaggt ttgcctcag tttgttaaa 
      gatttacat ttgaaaggg atgcattat ataccgttg 
      attgacgcg ggggttagt gggagtgag cctccaggg 
      tcgtacccc ccatttgac gaaggcctc aaaatggat 
      atttttgta aaaaattcg agtggaaaa atttttatt 
      gggaaagtt tggaataac aaaactaca gtttggccc 
      gactttaca catcctacg actgtggac tattggaca 
      atgatgctg aagtcgctc catgatatt gtacccttc 
      gatggggcc tggattgac atgaatgaa ccttcgaat 
      ttcctctcg ggctcgttt aatgggtgc cccaagact 
      agtctagac agcccccct tacttgcca agtgtcgac 
      ggtggcgcc ctcaattac aaaacaatg tgcatgtcg 
      gcgaaacac tacgctggg ttgcactac aacgtgcac 
      aatttgttt ggtttcacc gaggcaatc gttacaagt 
      ttcgccatg tctgacatt cgggggcgc cgccctatg 
      gtcatatcc cgctcgacc ttcgccggc catggccac 
      tatgccggt cactggagc ggtgacgtc gtctctgat 
      tggctagac atgagatac accattcca caattatta 
      agtttcagc cttttcgga gtcccttta atgggggcc 
      gacatttgt ggcttcaac ggcaacacc actaggagt 
      ttgtgtaac cggtggacc caactgggg gctttttat 
      cccttttcg cggaaccac aacacggac gatggcatt 
      gatcaagac ccggtcgcc atgggcccc gaagtggtc 
      atgtcggcc cgcaaggcc ctctcgatg cgctacaaa 
      ctgctcccc tacttgtac actttgttc tgggcggcg 
      cacactaga ggcgacacc gtcgcgcgt cccctattt 
      tttgaattt ccaaccgat ttgaaaact tacgatatc 
      gatacgcaa tttttgtgg ggccctgct ttgatgatt 
      gttcccgtt ttagaggag aattcgacc gaagtcacg 
      gcctacttg ccggaaggg ttgtggtac gatatttac 
      accaaatca ccaattgca ggccaaggc caaagtgtg 
      aatctctca gcccccttg gatacgatc ccggttttg 
      ttacgaggg ggctatatc ttgccaacg caagcccct 
      gaacagacc acaacccgc tcgcggtta aaccgaatt 
      gagatcgta gcagccggc gatgaacaa atgaatgcg 
      tttggggag ttttattgg gatgatggc gattcgttg 
      aattcctac gaggaaaaa caatacact ttgattgat 
      ttttggatg gagaggggg gtcctaaga agcgagaat 
      atcttttgg agtgggaca gaaaacccc ccaaatttg 
      ggcgcagtg actatcgtt ggtattact gattcggtc 
      gaggaggtt ttggtgaat aatgtggcg caagctttc 
      cgttacgac actatccat aagtatttg ttgattgaa 
      aatctgaat gttgaatta cagaaacct gtagtcgtt 
      acatggaaa taa
    mRNA sequence
    • >TC002741 RA
      gtcaaccaa aaagtgagg ttatgagta aacaacaaa 
      tctgaaatg atcccatta tcaacaaca aacaccagt 
      caaatcgct taaactaac aaaaaatca taatcagtg 
      cgcgagagt ttaataaac tcgccttcc tctcatagt 
      gtatcacag gatgtaaca ttcctgtta agtttaatc 
      gtcgcaaaa tcatttgca aaaaaatga cgcgaattc 
      gttatttgc tgcaagcat tccggtccc ggcggcgta 
      accgccact tatcccgca ctgattccg tgtctttcg 
      aggccttct gtaacgaaa tcccgccac agaagtgcc 
      ccgcaacca aagaatcat ccctaaatg gcgacttca 
      actcctcac cgctcaaaa gagaacgcc tggaggaag 
      attacgtcc gcttcgccc cacagaact gcgaatcca 
      ccacttgct cccgtccta gtctgccgg caatcctgc 
      tggtgctca tgcttctgg ctgtgtttt tagtccttc 
      ccttggtat atttgttac attgttttg gagttattt 
      acactcaag aacgctacg aacacccga ctttactcg 
      aaaaaatcc accacacgc acaaatcca gcgaaacca 
      aagttccta cagctctgc cgccgcccc cgaaaccgg 
      attatggta aatgcaaac tggtccaag agcgcgatc 
      ggttcgatt gctatcctg agaatgggg ccaatcagc 
      aggggtgcg aggccaggg gctgctgct ggatcccgg 
      cgaaaaaga agccgaaaa tgggggtgc ccctggcca 
      ccccttact gtttctatc cctccaatt acgggacct 
      ataattacg taaatgtga cgcagacgg cctacggtc 
      tggaggcgt ttttgaaaa gggggtacg caacggcct 
      atcctggag atgttgaaa ttattaaac ttagtgcca 
      agtttgaga cggaaacga ggctgcata ttaagattt 
      cagatccgc tgaaaaaca ggtttgaac ccccttttc 
      ctgaagttc ctatcgtcg ataaagcgg ccatgaatt 
      tgagttacc tgttttata ttgactcga ctaagcccg 
      gttttcggg tggtaagac gctcagata ataccataa 
      tctttgacg cattaagtt tgccaaatt taatttttt 
      ctgatcagt ttttacaac tgagcggta agttaccgt 
      ctaattata tttacggaa ttggagaac accgcactc 
      gtttattgt tatcgaccc aatggtcca gattcacgt 
      tattcaacc atgacgcaa taccatctt ttgaaaaaa 
      atttatacg gcagtcatc ctttttatc taattatgg 
      aaaactcga ctaaaagcc acggttttt acctccaaa 
      actccaacg ctatggatg ttattctcc aacccaccc 
      ccgctataa cattccgac caatcggcg gtgtcctag 
      acttctatt ttttccttg gccccaccc ccagtgacg 
      taatcagcc aatacacgg atttgattg ggcggccgt 
      tcatgccgc catattggg ggttgggct ttcatttat 
      gtcggttcg gttacaaaa ctttgaacc gaactaaac 
      tcgtgatgc aacgtaaca tcgatgccg ggattccat 
      tggacacac aatggaacg atttggact acatgaaaa 
      gtagcaacg attttactt acgattccg tcaatttca 
      aaggtttgc ctcagtttg ttaaagatt tacatttga 
      aagggatgc attatatac cgttgattg acgcggggg 
      ttagtggga gtgagcctc cagggtcgt accccccat 
      ttgacgaag gcctcaaaa tggatattt ttgtaaaaa 
      attcgagtg gaaaaattt ttattggga aagtttgga 
      ataacaaaa ctacagttt ggcccgact ttacacatc 
      ctacgactg tggactatt ggacaatga tgctgaagt 
      cgctccatg atattgtac ccttcgatg gggcctgga 
      ttgacatga atgaacctt cgaatttcc tctcgggct 
      cgtttaatg ggtgcccca agactagtc tagacagcc 
      ccccttact tgccaagtg tcgacggtg gcgccctca 
      attacaaaa caatgtgca tgtcggcga aacactacg 
      ctgggttgc actacaacg tgcacaatt tgtttggtt 
      tcaccgagg caatcgtta caagtttcg ccatgtctg 
      acattcggg ggcgccgcc ctatggtca tatcccgct 
      cgaccttcg ccggccatg gccactatg ccggtcact 
      ggagcggtg acgtcgtct ctgattggc tagacatga 
      gatacacca ttccacaat tattaagtt tcagccttt 
      tcggagtcc ctttaatgg gggccgaca tttgtggct 
      tcaacggca acaccacta ggagtttgt gtaaccggt 
      ggacccaac tgggggctt tttatccct tttcgcgga 
      accacaaca cggacgatg gcattgatc aagacccgg 
      tcgccatgg gccccgaag tggtcatgt cggcccgca 
      aggccctct cgatgcgct acaaactgc tcccctact 
      tgtacactt tgttctggg cggcgcaca ctagaggcg 
      acaccgtcg cgcgtcccc tattttttg aatttccaa 
      ccgatttga aaacttacg atatcgata cgcaatttt 
      tgtggggcc ctgctttga tgattgttc ccgttttag 
      aggagaatt cgaccgaag tcacggcct acttgccgg 
      aagggttgt ggtacgata tttacacca aatcaccaa 
      ttgcaggcc aaggccaaa gtgtgaatc tctcagccc 
      ccttggata cgatcccgg ttttgttac gagggggct 
      atatcttgc caacgcaag cccctgaac agaccacaa 
      cccgctcgc ggttaaacc gaattgaga tcgtagcag 
      ccggcgatg aacaaatga atgcgtttg gggagtttt 
      attgggatg atggcgatt cgttgaatt cctacgagg 
      aaaaacaat acactttga ttgattttt ggatggaga 
      ggggggtcc taagaagcg agaatatct tttggagtg 
      ggacagaaa accccccaa atttgggcg cagtgacta 
      tcgttggta ttactgatt cggtcgagg aggttttgg 
      tgaataatg tggcgcaag ctttccgtt acgacacta 
      tccataagt atttgttga ttgaaaatc tgaatgttg 
      aattacaga aacctgtag tcgttacat ggaaataag 
      tgtttctgg tacacaatt tgccaaaga attacgcgc 
      tgttgtatt agtgttatt ttaaggctg ttttttcaa 
      attgactga tgttagggt tagatattg cttggatgt 
      aattgccgt tttaccttt cgaaaataa acaataaat 
      aagtaaata aagatatca agttgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY