Status
Please wait ...
Gene information
General information
TC003651 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11598255..11605179
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003651
    iBeetle screen iB_02711

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003651 RA
      MQKNLLKIL GVSLIIFTI SEAASSKLK DQQTDLVVA 
      KRSLLERER EFFKTRPMS PDLRSFIQR YKRKNFDKR 
      QSEKRNKRS QEIAENQLY IVQNGQLVP LRNMKGFKR 
      SEEKKPEPE GFTVVPLVI PINMKNPAV KFVKTRMLS 
      EENAQLKSG AVPAGQAGG GAPEASTPA PQNEEAKPP 
      EVEEMISGE EENSNSTRE EKEVLHKIL QDVEGLMQR 
      KSEGNKKEG VICNINGSW ISDAAGMRF DVCYQNSTR 
      KKFRIHVAD RIPPNEDGF LCDGNWTVF GEIPFMHSG 
      MVIALAVHE KDRHIATFI GECRICEGS ETVTGTWLV 
      ARSSRDCKD QKAAHKVLS DVWKREQAH TLRKKHLQK 
      IGIAMDGED
    CDS sequence
    • >TC003651 RA
      atgcaaaaa aatctactc aaaattcta ggagtcagc 
      ttaataatc ttcacgatt tcagaagcg gcatcatct 
      aaacttaag gaccaacaa accgacctc gtcgtagct 
      aaacgctcc ctcctcgaa agagaacgt gagtttttc 
      aagacacgt ccgatgagt cccgatctc cgttccttc 
      atccaaaga tacaaacga aaaaatttc gacaagaga 
      caaagcgaa aaacggaac aaacgctcg caagaaatc 
      gccgaaaat cagctgtac atcgtccag aacggccag 
      ttggtgcct ctgaggaac atgaaaggg ttcaaacga 
      agcgaggag aagaagccg gaaccggaa gggttcact 
      gtggttccg ttggtgatt cccatcaac atgaaaaat 
      ccagcagtg aagtttgtc aaaacgagg atgctgagc 
      gaggagaat gcacagctg aagagtggg gcggtgcca 
      gcggggcag gctgggggc ggcgccccg gaggcgagc 
      acccccgcg ccccaaaac gaggaggct aagcctcct 
      gaagtggaa gaaatgatc agtggcgaa gaggagaat 
      agtaatagt accagggag gaaaaggag gtcttgcat 
      aagatcctg caagatgtg gaagggcta atgcagcgg 
      aaaagcgaa gggaataag aaggaaggt gtcatttgc 
      aacatcaac ggatcttgg attagtgac gccgctggt 
      atgcgcttc gatgtttgt taccagaac tccacacga 
      aaaaaattt cgcatccat gttgcggat cgtattcca 
      ccgaacgaa gacggattt ctctgtgat gggaactgg 
      accgttttc ggggaaata cccttcatg cattcggga 
      atggtgatt gctctagca gtgcacgag aaggaccga 
      cacattgcc acgttcata ggagagtgt cgtatttgc 
      gaaggtagc gagaccgtg acaggcacg tggttggtg 
      gctcgaagt tcgcgcgat tgtaaagac caaaaagcc 
      gcacataaa gtcttaagc gacgtttgg aaacgggag 
      caagctcat actttaagg aagaaacac ttgcaaaaa 
      atcggaatt gcgatggat ggagaagat taa
    mRNA sequence
    • >TC003651 RA
      gtcattttg acgttttgt tgtccgagc cgttcttag 
      ccttgatgt ggcatgaaa ccaagcgct cttttacaa 
      taatatgca aaaaaatct actcaaaat tctaggagt 
      cagcttaat aatcttcac gatttcaga agcggcatc 
      atctaaact taaggacca acaaaccga cctcgtcgt 
      agctaaacg ctccctcct cgaaagaga acgtgagtt 
      tttcaagac acgtccgat gagtcccga tctccgttc 
      cttcatcca aagatacaa acgaaaaaa tttcgacaa 
      gagacaaag cgaaaaacg gaacaaacg ctcgcaaga 
      aatcgccga aaatcagct gtacatcgt ccagaacgg 
      ccagttggt gcctctgag gaacatgaa agggttcaa 
      acgaagcga ggagaagaa gccggaacc ggaagggtt 
      cactgtggt tccgttggt gattcccat caacatgaa 
      aaatccagc agtgaagtt tgtcaaaac gaggatgct 
      gagcgagga gaatgcaca gctgaagag tggggcggt 
      gccagcggg gcaggctgg gggcggcgc cccggaggc 
      gagcacccc cgcgcccca aaacgagga ggctaagcc 
      tcctgaagt ggaagaaat gatcagtgg cgaagagga 
      gaatagtaa tagtaccag ggaggaaaa ggaggtctt 
      gcataagat cctgcaaga tgtggaagg gctaatgca 
      gcggaaaag cgaagggaa taagaagga aggtgtcat 
      ttgcaacat caacggatc ttggattag tgacgccgc 
      tggtatgcg cttcgatgt ttgttacca gaactccac 
      acgaaaaaa atttcgcat ccatgttgc ggatcgtat 
      tccaccgaa cgaagacgg atttctctg tgatgggaa 
      ctggaccgt tttcgggga aataccctt catgcattc 
      gggaatggt gattgctct agcagtgca cgagaagga 
      ccgacacat tgccacgtt cataggaga gtgtcgtat 
      ttgcgaagg tagcgagac cgtgacagg cacgtggtt 
      ggtggctcg aagttcgcg cgattgtaa agaccaaaa 
      agccgcaca taaagtctt aagcgacgt ttggaaacg 
      ggagcaagc tcatacttt aaggaagaa acacttgca 
      aaaaatcgg aattgcgat ggatggaga agattaaat 
      cgctcgctc gctcgctcg tatttattt gtatattta 
      tattatatt ctacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY