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Gene information
General information
TC034596 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10621213..10631320
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034596
    iBeetle screen iB_02709

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    Adult morphology
    adult cuticle darker - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    adult pigmentation/tanning darker - Number of animals showing the phenotype: 10


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034596 RA
      MPATGEDQD LVQDLIEEP ASFSDAVLS SDEELFHQK 
      CPKPAPIYS PVSKPVSFE SLPNRRLHE EFLRSSVDV 
      LLQEAVFEG TNRKNRVLQ WREPEELRR LMDFGVRSA 
      PSTHEELLE VLKKVVTYS VKTGHPYFV NQLFSAVDP 
      YGLVAQWAT DALNPSVYT YEVSPVFVL MEEVVLREM 
      RAIVGFEGG KGDGIFCPG GSIANGYAI SCARYRFMP 
      DIKKKGLHS LPRLVLFTS EDAHYSIKK LASFQGIGT 
      DNVYLIRTD ARGRMDVSH LVEEIERSL REGAAPFMV 
      SATAGTTVI GAFDPIEKI ADVCQKYKL WLHVXMPPG 
      EVARLSLPN TATSSKGLR GPTRSPGTL TNS
    CDS sequence
    • >TC034596 RA
      atgccggcc acaggcgaa gaccaagac ctggtgcaa 
      gacctcatc gaggagccc gcctccttc agcgacgcc 
      gtcctctcc tccgacgag gaactcttc caccagaag 
      tgccccaaa cccgccccc atttactcc ccagtctcg 
      aaaccggtc tccttcgag agcctcccc aacaggcgc 
      ctccacgag gagttcctc cgcagctcg gtggacgtc 
      ctcctccag gaggcggtg ttcgaggga acgaaccgc 
      aagaaccgg gtgctgcaa tggcgggag ccggaggag 
      ttgaggcgt ctgatggac tttggggtg cggagtgcg 
      ccctccacg cacgaggag ttgttggag gtgttgaag 
      aaggttgta acttattcg gttaaaacc ggacatccg 
      tacttcgtg aaccagttg ttctcggcg gtggatccg 
      tacggtttg gtggcacaa tgggccacg gatgcgctc 
      aatccgagt gtttacacc tacgaggtt tcgccggtt 
      tttgttctg atggaggaa gtggttttg agggagatg 
      agggccatt gtggggttc gagggggga aagggcgat 
      gggattttt tgcccagga gggtccatt gccaatgga 
      tatgccatc agttgtgcc agatacagg tttatgccc 
      gatattaag aaaaaaggc ctccactct ctcccccgt 
      ttggtcctc ttcacctct gaagatgcc cactattcc 
      atcaaaaaa ctcgcctct ttccaaggc atcggcacc 
      gacaacgtc tacttgata cgaacggac gcccgaggt 
      cgcatggac gtctcgcac ctggtggag gaaatcgag 
      cgttcgctc cgtgaaggc gccgctcct ttcatggtc 
      agtgccacc gctggaacc acagtgatt ggtgccttt 
      gaccccatc gaaaaaatc gcagatgtg tgccaaaaa 
      tacaaactg tggttgcac gtgganatg ccgcctggg 
      gaggtggcg cgcttgtct ctgccaaac accgccacc 
      tcctcaaag ggattgaga gggccgact cggtcacct 
      ggaaccctc acaaactcc taa
    mRNA sequence
    • >TC034596 RA
      gcccccacg aggatccac acacgatgc cggccacag 
      gcgaagacc aagacctgg tgcaagacc tcatcgagg 
      agcccgcct ccttcagcg acgccgtcc tctcctccg 
      acgaggaac tcttccacc agaagtgcc ccaaacccg 
      cccccattt actccccag tctcgaaac cggtctcct 
      tcgagagcc tccccaaca ggcgcctcc acgaggagt 
      tcctccgca gctcggtgg acgtcctcc tccaggagg 
      cggtgttcg agggaacga accgcaaga accgggtgc 
      tgcaatggc gggagccgg aggagttga ggcgtctga 
      tggactttg gggtgcgga gtgcgccct ccacgcacg 
      aggagttgt tggaggtgt tgaagaagg ttgtaactt 
      attcggtta aaaccggac atccgtact tcgtgaacc 
      agttgttct cggcggtgg atccgtacg gtttggtgg 
      cacaatggg ccacggatg cgctcaatc cgagtgttt 
      acacctacg aggtttcgc cggtttttg ttctgatgg 
      aggaagtgg ttttgaggg agatgaggg ccattgtgg 
      ggttcgagg ggggaaagg gcgatggga ttttttgcc 
      caggagggt ccattgcca atggatatg ccatcagtt 
      gtgccagat acaggttta tgcccgata ttaagaaaa 
      aaggcctcc actctctcc cccgtttgg tcctcttca 
      cctctgaag atgcccact attccatca aaaaactcg 
      cctctttcc aaggcatcg gcaccgaca acgtctact 
      tgatacgaa cggacgccc gaggtcgca tggacgtct 
      cgcacctgg tggaggaaa tcgagcgtt cgctccgtg 
      aaggcgccg ctcctttca tggtcagtg ccaccgctg 
      gaaccacag tgattggtg cctttgacc ccatcgaaa 
      aaatcgcag atgtgtgcc aaaaataca aactgtggt 
      tgcacgtgg anatgccgc ctggggagg tggcgcgct 
      tgtctctgc caaacaccg ccacctcct caaagggat 
      tgagagggc cgactcggt cacctggaa ccctcacaa 
      actcctaac agcccccca gcaatgttc cacactttt 
      actgcgaca tgagggtgt cctcgccga ggcgcattc 
      cacgaacgc cgcttacct cttccaaaa agacaaatt 
      ctacgacac caaatacga cacgggcga caagcacat 
      ccagtgcgg ccgcagggc cgacgtcct caagttctg 
      gttcatgtg gaaggcgaa gggaacatc agggttgga 
      gaaacacgt cgataaagt gttcgaaaa tgcgagatt 
      tttcaccga ttgtataaa aaatcggga agggtttga 
      aatggtgat agcggagcc cgaatacac aaacatctg 
      cttttggta cgtgccgaa gagtctgag ggggcgcaa 
      ggacgaagc cgattacaa agacaagct gcataaggt 
      ggcccccag gattaagga gaggatgat gaaggaggg 
      ctccatgat ggtcacgta ccaggcgca aaagggaca 
      cccgaattt tttcaggat tgtgttcca gaattcggg 
      gcttgacaa ggctgatat ggtgcactt tgttgagga 
      gattgagcg gttggggag cgatcttta aggccttga 
      atggtgcta gttgtagat tgtgtaatt aatgtaaaa 
      agtattatt taaaaaatg taaattttg atgtattta 
      ttctcatta gttgtagtt tattcaaat aaaagttta 
      aaaaaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY