Status
Please wait ...
Gene information
General information
TC000052 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10792893..10800007
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000052
    iBeetle screen iB_02708

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000052 RA
      MSSISAQGV VERASAELS KRINGLGLR GSKHHSTKG 
      GSVMERVTN VFCGGGGGG SAAGQLVVD DKFLARFFL 
      YFSPCERCV LAQVCTRWR DVLYRSPRF WTGLVPVLQ 
      CREMRAAQG NERSRLYSS LLRRGFHNL CLMGASDED 
      ALDLINSFP LASKHIHTL SLRCSSITD RGLESLLDH 
      LQALYELEL AGCNEITEA GLWACLNPR IVSLTLSDC 
      INVADEAVG AVAQLLPAL YEFSLQAYH VTDAALGYF 
      SPKQSNSLN ILRLHSCWE ITNHGVVNI VHSLPNLTV 
      LSLSGCSKI TDDGVELIA ENLQKLRSL DLSWCPRIT 
      DAALEYIAC DLNQLEELT LDRCVHITD IGIGYISTM 
      LSLSALYLR WCSQIRDFG LQHLCGMRN LQILSLAGC 
      PLLTSSGLS SLIQLRHMR ELELTNCPG ASKELFDYL 
      REHLPRCLV IE
    CDS sequence
    • >TC000052 RA
      atgtcgtcg atatcggcc cagggggtg gtggagcgc 
      gcctcggcg gagctctcc aaacgcatc aacggcctg 
      ggcctccgc ggcagcaag caccactcg accaaaggg 
      ggcagcgtc atggagcgc gtcaccaac gtgttttgc 
      ggcggcggc ggcggcggg agcgcggcg ggccagctg 
      gtggtcgac gacaagttc ctcgccagg tttttcctg 
      tatttcagt ccgtgcgag cggtgcgtg ctggcgcag 
      gtgtgcact aggtggagg gacgtgttg tacaggtcg 
      ccgaggttt tggacgggg ctggtgccc gtgctgcag 
      tgccgggag atgagggcg gcgcaaggg aacgagcgc 
      tccaggttg tactcgtcg ctgctgagg agggggttt 
      cataatttg tgtctgatg ggggcttcc gatgaagat 
      gcgctcgat cttattaat tcgtttccg ttagcttcg 
      aaacatata catacgttg agtttgagg tgttcgagc 
      atcaccgat cggggactc gagtcgctg cttgaccat 
      ctgcaggcg ctctacgaa ttggaacta gcaggctgt 
      aatgaaata acggaggct ggtttatgg gcctgtctg 
      aatcctaga atcgtctct ttgacacta agtgactgt 
      ataaatgtt gccgatgaa gctgtaggc gctgtggct 
      caactcctc cctgcattg tacgaattc tccctccag 
      gcctaccat gtcacggac gccgcttta ggttacttt 
      tcgccaaaa caaagcaac tcactcaat atcctcaga 
      ctgcactcg tgttgggaa atcaccaat cacggagtt 
      gtaaatatt gttcattct ctcccgaat ctaacggtc 
      ctcagcctc tccggatgc agcaaaatc accgacgac 
      ggcgtcgaa ctcatagct gagaattta caaaaattg 
      agatcgctc gacttgtcg tggtgtcct cgaatcacc 
      gatgctgcc ttggagtat atcgcgtgc gatctcaac 
      caattagaa gagcttaca ctcgatcga tgtgttcac 
      atcaccgac attggcatt ggttacatt tcaacaatg 
      ttgtctcta tctgcactc tatctgcgc tggtgttca 
      caaattcga gactttgga cttcagcat ttatgcgga 
      atgagaaat ttgcaaata ctatcgtta gcagggtgt 
      ccattattg acttctagt ggtttatca agtctgatt 
      cagttacga catatgaga gaattagaa ttaacaaat 
      tgtccaggg gcatcaaaa gaacttttc gattatttg 
      agagaacat cttccgcgt tgcctcgtt atagaataa
    mRNA sequence
    • >TC000052 RA
      acgctagca attatcaca ttaataata attaattca 
      gataaatcc aaagacagt gcaaatcaa gttacgcaa 
      cgctttgtc cctccgaat ttatccgtt cgttttcgc 
      cgtttgtcg gcactcacc cccgttttc tcccgtatt 
      gatcggtcc atcagcagc gagggtgaa cgcgaccgt 
      atcgagtgg tgcagtgat cgcgatgcc ctcgtgagt 
      gcacccctt acacatcat gtcgtcgat atcggccca 
      gggggtggt ggagcgcgc ctcggcgga gctctccaa 
      acgcatcaa cggcctggg cctccgcgg cagcaagca 
      ccactcgac caaaggggg cagcgtcat ggagcgcgt 
      caccaacgt gttttgcgg cggcggcgg cggcgggag 
      cgcggcggg ccagctggt ggtcgacga caagttcct 
      cgccaggtt tttcctgta tttcagtcc gtgcgagcg 
      gtgcgtgct ggcgcaggt gtgcactag gtggaggga 
      cgtgttgta caggtcgcc gaggttttg gacggggct 
      ggtgcccgt gctgcagtg ccgggagat gagggcggc 
      gcaagggaa cgagcgctc caggttgta ctcgtcgct 
      gctgaggag ggggtttca taatttgtg tctgatggg 
      ggcttccga tgaagatgc gctcgatct tattaattc 
      gtttccgtt agcttcgaa acatataca tacgttgag 
      tttgaggtg ttcgagcat caccgatcg gggactcga 
      gtcgctgct tgaccatct gcaggcgct ctacgaatt 
      ggaactagc aggctgtaa tgaaataac ggaggctgg 
      tttatgggc ctgtctgaa tcctagaat cgtctcttt 
      gacactaag tgactgtat aaatgttgc cgatgaagc 
      tgtaggcgc tgtggctca actcctccc tgcattgta 
      cgaattctc cctccaggc ctaccatgt cacggacgc 
      cgctttagg ttacttttc gccaaaaca aagcaactc 
      actcaatat cctcagact gcactcgtg ttgggaaat 
      caccaatca cggagttgt aaatattgt tcattctct 
      cccgaatct aacggtcct cagcctctc cggatgcag 
      caaaatcac cgacgacgg cgtcgaact catagctga 
      gaatttaca aaaattgag atcgctcga cttgtcgtg 
      gtgtcctcg aatcaccga tgctgcctt ggagtatat 
      cgcgtgcga tctcaacca attagaaga gcttacact 
      cgatcgatg tgttcacat caccgacat tggcattgg 
      ttacatttc aacaatgtt gtctctatc tgcactcta 
      tctgcgctg gtgttcaca aattcgaga ctttggact 
      tcagcattt atgcggaat gagaaattt gcaaatact 
      atcgttagc agggtgtcc attattgac ttctagtgg 
      tttatcaag tctgattca gttacgaca tatgagaga 
      attagaatt aacaaattg tccaggggc atcaaaaga 
      acttttcga ttatttgag agaacatct tccgcgttg 
      cctcgttat agaataagt gaaccactt gtccacttg 
      ttatatttg atactagat tgtgcaata acac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY