Status
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Gene information
General information
TC031042
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000053 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10801335..10808413
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031042
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02707

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 66.67% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Adult morphology
    elytra blistered - Number of animals showing the phenotype: 2

    elytra slightly surface irregular - Number of animals showing the phenotype: 5


    elytra wing veins slightly pattern irregular - Number of animals showing the phenotype: 5

    Fertility
    egg production: number no/almost no eggs -
    ovariole not present - Number of animals showing the phenotype: 3

    Comment: just a small remnant of some ovarioles is left, terminal filaments are there
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031042 RA
      MATREKDPF DSDEQKHSD SDGAPKQTF EPEIDNPEM 
FMKPACECQ NCVHWTQSA DWTTPDLEF EGHHKRVCK 
ERKIQPLLR SARHPGKTP KIFTFNPGT LTKIEKQSY 
LKTLVHGEV IPQPVKLLT DTYFSDEIQ AALGRLNYK 
YSLPIQSFV WPAIFRQLN VVMVGGPKS GKTMSYLPA 
LCTFSTAEE EKYRQLSKY KGPLVVIIC PNSKHCEDV 
YDLMKKLFE NSATKPKIA LMTYPVHHI NSVDIVITI 
PSTLTHCLK SRTFNLKRM CHLVLEDAS CLLKNNHEE 
IYKILEVTD EMLEHRMCS TKVQFIVAS TKWTSDLQK 
LLKSIYVTP LVCIGNYLE AALYGNIQF EVKFLVSQN 
KKHYIEGLL RDKSHLFKT IVVCHNNEI SDLNKWLVR 
NGIEVMALN DDSTKEQIE VCEEEWAFM RGGQYKVLL 
CTDFIFDSL LCITDAMWL VSYSFSATW TRFIKRFSA 
LSSHYKSPW ETNEEEVKP KCFLMIDEN CEQQMTKVL 
NLLNRLSKD LPPSYQKYA SAFKSKTEN EKIDNGIEI 
CNTLKLFGK CGDSLCEKR HFVCEQLDV SENLPKSGK 
LVFKILNIK DVTQFSVQV IRHIDLEGK VKEINDDDL 
RKPLTVSEK RAKNIKVGK FYAHCDYTP EGDVFMLCH 
VLSIPNDNF VEVKFANEL VLQTLQRKL YELPPNLEI 
VDVIMCNLI PPFKDEMFS ARSFHVTHN SLQTVEYKN 
MILTGDIYL QLDRTFWLN NIAQVLQVD GCDIIKFNL 
TKHLTNLKV VELSSNQIT SLHKLCEKC GITLPCYLK 
HTAKKASKA KEVEAQRAF LDDNVHEVI LSSAVSPEE 
FYVRLAKFQ PLLENLERD IQESISKGI PEKHLNIEI 
GKYYLALDS HYELYSRVL ILNIQDSKA LCFYVDYGD 
EAIVELSKL KMLPSKFLI RLPFQAIQC RLYGLSPIS 
GEWDDEATD VLYKYMFEP QSDVFRSIY VQTLCKDAS 
ETMRVKTTY SVLMKDGFG DNNVLINQL MLDCGFATS 
IFDKIDDFE IPRPEAVEE ESSDSDAGC LEVGPEEPN 
LEEIFGDDF DLEIIDAEE FLEVLGVAR RENPSLALE 
NSDSNHTET IHDPINSQI DQTVEKSNC LTPDVYWSQ 
TENTVKLKI KLIGVENYK TNVVQKRIF TFSSLLNEK 
NYELKFTLH KPVKMANHR ALGQEVVVV LNKLEPVEW 
LQLTGTQRR MRFVHYEVP EFKEEAKRK LLQLDITDD 
ESDENDGEA PMYHVISDM DSEFDEEID SSESDL
    CDS sequence
    • >TC031042 RA
      atggccacc agggagaaa gaccctttc gacagcgac 
gagcaaaaa cactccgat agtgatgga gccccaaag 
cagactttt gagcccgaa atcgacaat ccagagatg 
ttcatgaaa ccggcgtgt gagtgccag aactgcgtc 
cattggacc caaagcgcc gactggaca acgccggat 
ttggagttc gaggggcac cacaaacgt gtttgcaaa 
gagcggaag attcaaccc ttgttacga tcggcacga 
catccaggg aaaacccct aagatcttc actttcaat 
cctggcaca ttgacaaaa attgagaaa caaagctac 
ttgaagacc ctagttcac ggggaggtg atcccccag 
cctgtaaag ttgctaaca gacacctac tttagcgac 
gaaattcaa gctgctttg ggtcgcctt aactacaag 
tatagttta ccaatccaa tcattcgtc tggcccgca 
attttcaga caattgaat gttgttatg gtagggggg 
cctaagtcg gggaaaacc atgtcgtat ctcccggcc 
ctttgtacc ttcagtaca gctgaagag gaaaaatat 
cgacaatta tcaaagtac aagggcccg ttagtggtt 
attatttgt ccaaacagc aaacattgt gaagatgta 
tacgatttg atgaaaaaa ctgtttgaa aatagtgcc 
actaaacca aaaattgcc ctcatgaca tatccagtc 
caccacatt aacagcgtt gatatcgtg attacaatc 
cccagcact cttacacat tgccttaaa tctcggact 
tttaatttg aaacgaatg tgtcacttg gtgttagaa 
gacgcgagt tgtttacta aaaaacaat cacgaagaa 
atttacaaa attttggaa gtcactgat gaaatgctt 
gaacacagg atgtgtagc accaaagtc caattcata 
gtagcttca acaaaatgg acatcggat ttgcaaaaa 
ttactcaaa tcgatttat gtaacacca cttgtttgc 
atagggaat tatttggaa gcagctcta tatgggaac 
atccaattc gaagtaaag tttttagtg tcacaaaac 
aagaaacac tacattgaa ggattatta agagacaag 
agtcatctt tttaaaact attgttgtg tgccataat 
aatgaaata agcgatttg aataaatgg ttggttcga 
aacggcatc gaagtcatg gctttaaat gatgattcg 
actaaggaa caaatcgag gtttgtgag gaagaatgg 
gcttttatg agaggtggc caatacaaa gtgttactg 
tgcacggat tttattttc gactcatta ttgtgcatc 
accgacgcc atgtggctc gtcagttac tcattttcc 
gcaacttgg acccgattt atcaaacgt ttcagcgct 
ttgtcctca cattacaag agtccttgg gaaacaaat 
gaagaagaa gtcaaaccc aaatgtttt ttgatgatt 
gacgaaaac tgcgaacaa caaatgaca aaagtcctc 
aatttactc aaccgcttg agcaaggat ttgcctcca 
agttaccaa aaatacgcc agtgcgttc aagagtaaa 
actgaaaat gagaaaatc gacaatggg attgagatt 
tgtaacaca ttgaaactg tttgggaaa tgtggcgat 
tccttgtgt gagaagcga catttcgtt tgtgaacaa 
ctggacgtt tcggaaaat ctgccaaag agtggaaaa 
ctcgtattt aagattctt aatattaaa gatgtgaca 
cagttttcc gtccaggta attagacac atcgatttg 
gaggggaaa gtgaaggaa attaatgat gacgatttg 
agaaaaccc ctcacagtt tcagaaaaa cgtgccaaa 
aacattaaa gtgggaaaa ttttacgca cattgtgac 
tacacccct gaaggtgat gtgttcatg ttgtgtcac 
gtgttgagc atccccaac gataatttc gttgaagtc 
aaatttgca aatgagttg gttttgcaa actttacag 
agaaaactg tacgagctt ccgccaaat ttagagatc 
gtggatgtc attatgtgc aatcttatt ccccccttc 
aaagatgaa atgttttca gcgcgatct tttcacgtc 
actcacaat tccctacaa actgtcgaa tataaaaat 
atgatttta accggtgac atctatctg caattggat 
cgaactttc tggttgaat aacatcgct caagtttta 
caagtcgat ggttgcgac atcattaaa tttaatcta 
actaagcat ttaacgaat ttaaaagtc gtcgaatta 
tcttcgaat caaatcacc agcctgcac aaactatgt 
gagaaatgt ggcataacc ctcccttgc tacctcaaa 
cacactgca aaaaaggcc tcgaaagca aaagaagtc 
gaagcgcaa cgagccttc ttagacgat aatgtccac 
gaagtgatc ctcagctca gccgtcagt cctgaggag 
ttctacgtc cgattggcc aaatttcaa ccactgttg 
gagaacttg gaaagggac attcaggag agcatcagt 
aagggaatc cccgagaag catttgaac atcgaaatc 
ggaaagtac tacctggcc ttggactct cactacgag 
ttgtattcg cgcgtcctc attttaaat atccaagat 
tcgaaggcg ctttgtttt tacgtggat tacggtgac 
gaagccata gtcgagctt tcgaaactg aaaatgttg 
ccatcgaaa ttcctaatt cgattgccg tttcaagcg 
attcagtgt cgcttgtat gggttgagc cccatttcg 
ggcgaatgg gacgatgaa gccactgat gttctatac 
aagtacatg tttgaaccc cagtcagat gttttccgg 
tcaatatac gtacaaacg ttatgtaag gatgcttcc 
gagacgatg agagttaaa acgacttat tccgtcttg 
atgaaggac gggtttggc gataacaac gttttgatc 
aaccagttg atgttggat tgtgggttt gcgacgtcg 
atttttgac aaaattgat gattttgag ataccgaga 
ccggaggcc gtggaggag gaatcgtcg gattcggat 
gctgggtgt ttggaggtg ggtccagaa gaacctaat 
cttgaagaa atttttgga gacgatttt gatttggaa 
ataattgac gccgaggaa tttctcgaa gttttgggg 
gttgcacgt cgtgaaaat ccctcctta gctttagaa 
aatagtgat tcaaatcac actgaaacc attcatgat 
ccgatcaat tctcaaatt gaccagact gttgagaaa 
agtaattgt ctcacgccg gacgtttat tggtcacaa 
accgaaaac accgtcaag cttaaaatc aagctaatc 
ggtgtcgaa aattacaaa acgaacgtg gttcagaaa 
cgaattttt acatttagt tcgcttctc aatgagaaa 
aattacgag ctgaagttt acgttacac aaaccggtc 
aagatggca aatcatcgg gcgttgggg caagaagtt 
gtggttgtt ttgaataaa ttggagcct gtggagtgg 
ttacagttg acgggcact caaagacgc atgaggttt 
gttcattac gaagtgcct gagtttaaa gaagaagcc 
aaacgaaag ctcctccag cttgatatt acggacgat 
gagagcgac gaaaatgac ggggaagca ccgatgtat 
catgtgata tccgacatg gatagcgag tttgatgaa 
gagatcgat tcgagtgag tccgatcta tag
    mRNA sequence
    • >TC031042 RA
      cctagaatt aaataattt ttgttgtgt tttgttgtg 
gtgtgtttt tgtgaattt gcacacgtt tttgcccta 
cttaaggcc tttcagtcg caatggcca ccagggaga 
aagaccctt tcgacagcg acgagcaaa aacactccg 
atagtgatg gagccccaa agcagactt ttgagcccg 
aaatcgaca atccagaga tgttcatga aaccggcgt 
gtgagtgcc agaactgcg tccattgga cccaaagcg 
ccgactgga caacgccgg atttggagt tcgaggggc 
accacaaac gtgtttgca aagagcgga agattcaac 
ccttgttac gatcggcac gacatccag ggaaaaccc 
ctaagatct tcactttca atcctggca cattgacaa 
aaattgaga aacaaagct acttgaaga ccctagttc 
acggggagg tgatccccc agcctgtaa agttgctaa 
cagacacct actttagcg acgaaattc aagctgctt 
tgggtcgcc ttaactaca agtatagtt taccaatcc 
aatcattcg tctggcccg caattttca gacaattga 
atgttgtta tggtagggg ggcctaagt cggggaaaa 
ccatgtcgt atctcccgg ccctttgta ccttcagta 
cagctgaag aggaaaaat atcgacaat tatcaaagt 
acaagggcc cgttagtgg ttattattt gtccaaaca 
gcaaacatt gtgaagatg tatacgatt tgatgaaaa 
aactgtttg aaaatagtg ccactaaac caaaaattg 
ccctcatga catatccag tccaccaca ttaacagcg 
ttgatatcg tgattacaa tccccagca ctcttacac 
attgcctta aatctcgga cttttaatt tgaaacgaa 
tgtgtcact tggtgttag aagacgcga gttgtttac 
taaaaaaca atcacgaag aaatttaca aaattttgg 
aagtcactg atgaaatgc ttgaacaca ggatgtgta 
gcaccaaag tccaattca tagtagctt caacaaaat 
ggacatcgg atttgcaaa aattactca aatcgattt 
atgtaacac cacttgttt gcataggga attatttgg 
aagcagctc tatatggga acatccaat tcgaagtaa 
agtttttag tgtcacaaa acaagaaac actacattg 
aaggattat taagagaca agagtcatc tttttaaaa 
ctattgttg tgtgccata ataatgaaa taagcgatt 
tgaataaat ggttggttc gaaacggca tcgaagtca 
tggctttaa atgatgatt cgactaagg aacaaatcg 
aggtttgtg aggaagaat gggctttta tgagaggtg 
gccaataca aagtgttac tgtgcacgg attttattt 
tcgactcat tattgtgca tcaccgacg ccatgtggc 
tcgtcagtt actcatttt ccgcaactt ggacccgat 
ttatcaaac gtttcagcg ctttgtcct cacattaca 
agagtcctt gggaaacaa atgaagaag aagtcaaac 
ccaaatgtt ttttgatga ttgacgaaa actgcgaac 
aacaaatga caaaagtcc tcaatttac tcaaccgct 
tgagcaagg atttgcctc caagttacc aaaaatacg 
ccagtgcgt tcaagagta aaactgaaa atgagaaaa 
tcgacaatg ggattgaga tttgtaaca cattgaaac 
tgtttggga aatgtggcg attccttgt gtgagaagc 
gacatttcg tttgtgaac aactggacg tttcggaaa 
atctgccaa agagtggaa aactcgtat ttaagattc 
ttaatatta aagatgtga cacagtttt ccgtccagg 
taattagac acatcgatt tggagggga aagtgaagg 
aaattaatg atgacgatt tgagaaaac ccctcacag 
tttcagaaa aacgtgcca aaaacatta aagtgggaa 
aattttacg cacattgtg actacaccc ctgaaggtg 
atgtgttca tgttgtgtc acgtgttga gcatcccca 
acgataatt tcgttgaag tcaaatttg caaatgagt 
tggttttgc aaactttac agagaaaac tgtacgagc 
ttccgccaa atttagaga tcgtggatg tcattatgt 
gcaatctta ttcccccct tcaaagatg aaatgtttt 
cagcgcgat cttttcacg tcactcaca attccctac 
aaactgtcg aatataaaa atatgattt taaccggtg 
acatctatc tgcaattgg atcgaactt tctggttga 
ataacatcg ctcaagttt tacaagtcg atggttgcg 
acatcatta aatttaatc taactaagc atttaacga 
atttaaaag tcgtcgaat tatcttcga atcaaatca 
ccagcctgc acaaactat gtgagaaat gtggcataa 
ccctccctt gctacctca aacacactg caaaaaagg 
cctcgaaag caaaagaag tcgaagcgc aacgagcct 
tcttagacg ataatgtcc acgaagtga tcctcagct 
cagccgtca gtcctgagg agttctacg tccgattgg 
ccaaatttc aaccactgt tggagaact tggaaaggg 
acattcagg agagcatca gtaagggaa tccccgaga 
agcatttga acatcgaaa tcggaaagt actacctgg 
ccttggact ctcactacg agttgtatt cgcgcgtcc 
tcattttaa atatccaag attcgaagg cgctttgtt 
tttacgtgg attacggtg acgaagcca tagtcgagc 
tttcgaaac tgaaaatgt tgccatcga aattcctaa 
ttcgattgc cgtttcaag cgattcagt gtcgcttgt 
atgggttga gccccattt cgggcgaat gggacgatg 
aagccactg atgttctat acaagtaca tgtttgaac 
cccagtcag atgttttcc ggtcaatat acgtacaaa 
cgttatgta aggatgctt ccgagacga tgagagtta 
aaacgactt attccgtct tgatgaagg acgggtttg 
gcgataaca acgttttga tcaaccagt tgatgttgg 
attgtgggt ttgcgacgt cgatttttg acaaaattg 
atgattttg agataccga gaccggagg ccgtggagg 
aggaatcgt cggattcgg atgctgggt gtttggagg 
tgggtccag aagaaccta atcttgaag aaatttttg 
gagacgatt ttgatttgg aaataattg acgccgagg 
aatttctcg aagttttgg gggttgcac gtcgtgaaa 
atccctcct tagctttag aaaatagtg attcaaatc 
acactgaaa ccattcatg atccgatca attctcaaa 
ttgaccaga ctgttgaga aaagtaatt gtctcacgc 
cggacgttt attggtcac aaaccgaaa acaccgtca 
agcttaaaa tcaagctaa tcggtgtcg aaaattaca 
aaacgaacg tggttcaga aacgaattt ttacattta 
gttcgcttc tcaatgaga aaaattacg agctgaagt 
ttacgttac acaaaccgg tcaagatgg caaatcatc 
gggcgttgg ggcaagaag ttgtggttg ttttgaata 
aattggagc ctgtggagt ggttacagt tgacgggca 
ctcaaagac gcatgaggt ttgttcatt acgaagtgc 
ctgagttta aagaagaag ccaaacgaa agctcctcc 
agcttgata ttacggacg atgagagcg acgaaaatg 
acggggaag caccgatgt atcatgtga tatccgaca 
tggatagcg agtttgatg aagagatcg attcgagtg 
agtccgatc tataggtct ataattgat tttttgaca 
ttattatta ccaaagttt atttcttgt tttattatg 
accaaactt taatttttt taataaatt cgacgattt 
gat
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