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Gene information
General information
TC031042
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000053
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 6240048..6247126
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031042
    iBeetle screen iB_02707
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not closed - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 66.67% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    Adult morphology
    elytra blistered - Number of animals showing the phenotype: 2

    elytra slightly surface irregular - Number of animals showing the phenotype: 5


    elytra wing veins slightly pattern irregular - Number of animals showing the phenotype: 5

    Fertility
    egg production: number no/almost no eggs -
    ovariole not present - Number of animals showing the phenotype: 3

    Comment: just a small remnant of some ovarioles is left, terminal filaments are there
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031042 RA
      MATREKDPF DSDEQKHSD SDGAPKQTF EPEIDNPEM 
      FMKPACECQ NCVHWTQSA DWTTPDLEF EGHHKRVCK 
      ERKIQPLLR SARHPGKTP KIFTFNPGT LTKIEKQSY 
      LKTLVHGEV IPQPVKLLT DTYFSDEIQ AALGRLNYK 
      YSLPIQSFV WPAIFRQLN VVMVGGPKS GKTMSYLPA 
      LCTFSTAEE EKYRQLSKY KGPLVVIIC PNSKHCEDV 
      YDLMKKLFE NSATKPKIA LMTYPVHHI NSVDIVITI 
      PSTLTHCLK SRTFNLKRM CHLVLEDAS CLLKNNHEE 
      IYKILEVTD EMLEHRMCS TKVQFIVAS TKWTSDLQK 
      LLKSIYVTP LVCIGNYLE AALYGNIQF EVKFLVSQN 
      KKHYIEGLL RDKSHLFKT IVVCHNNEI SDLNKWLVR 
      NGIEVMALN DDSTKEQIE VCEEEWAFM RGGQYKVLL 
      CTDFIFDSL LCITDAMWL VSYSFSATW TRFIKRFSA 
      LSSHYKSPW ETNEEEVKP KCFLMIDEN CEQQMTKVL 
      NLLNRLSKD LPPSYQKYA SAFKSKTEN EKIDNGIEI 
      CNTLKLFGK CGDSLCEKR HFVCEQLDV SENLPKSGK 
      LVFKILNIK DVTQFSVQV IRHIDLEGK VKEINDDDL 
      RKPLTVSEK RAKNIKVGK FYAHCDYTP EGDVFMLCH 
      VLSIPNDNF VEVKFANEL VLQTLQRKL YELPPNLEI 
      VDVIMCNLI PPFKDEMFS ARSFHVTHN SLQTVEYKN 
      MILTGDIYL QLDRTFWLN NIAQVLQVD GCDIIKFNL 
      TKHLTNLKV VELSSNQIT SLHKLCEKC GITLPCYLK 
      HTAKKASKA KEVEAQRAF LDDNVHEVI LSSAVSPEE 
      FYVRLAKFQ PLLENLERD IQESISKGI PEKHLNIEI 
      GKYYLALDS HYELYSRVL ILNIQDSKA LCFYVDYGD 
      EAIVELSKL KMLPSKFLI RLPFQAIQC RLYGLSPIS 
      GEWDDEATD VLYKYMFEP QSDVFRSIY VQTLCKDAS 
      ETMRVKTTY SVLMKDGFG DNNVLINQL MLDCGFATS 
      IFDKIDDFE IPRPEAVEE ESSDSDAGC LEVGPEEPN 
      LEEIFGDDF DLEIIDAEE FLEVLGVAR RENPSLALE 
      NSDSNHTET IHDPINSQI DQTVEKSNC LTPDVYWSQ 
      TENTVKLKI KLIGVENYK TNVVQKRIF TFSSLLNEK 
      NYELKFTLH KPVKMANHR ALGQEVVVV LNKLEPVEW 
      LQLTGTQRR MRFVHYEVP EFKEEAKRK LLQLDITDD 
      ESDENDGEA PMYHVISDM DSEFDEEID SSESDL
    CDS sequence
    • >TC031042 RA
      atggccacc agggagaaa gaccctttc gacagcgac 
      gagcaaaaa cactccgat agtgatgga gccccaaag 
      cagactttt gagcccgaa atcgacaat ccagagatg 
      ttcatgaaa ccggcgtgt gagtgccag aactgcgtc 
      cattggacc caaagcgcc gactggaca acgccggat 
      ttggagttc gaggggcac cacaaacgt gtttgcaaa 
      gagcggaag attcaaccc ttgttacga tcggcacga 
      catccaggg aaaacccct aagatcttc actttcaat 
      cctggcaca ttgacaaaa attgagaaa caaagctac 
      ttgaagacc ctagttcac ggggaggtg atcccccag 
      cctgtaaag ttgctaaca gacacctac tttagcgac 
      gaaattcaa gctgctttg ggtcgcctt aactacaag 
      tatagttta ccaatccaa tcattcgtc tggcccgca 
      attttcaga caattgaat gttgttatg gtagggggg 
      cctaagtcg gggaaaacc atgtcgtat ctcccggcc 
      ctttgtacc ttcagtaca gctgaagag gaaaaatat 
      cgacaatta tcaaagtac aagggcccg ttagtggtt 
      attatttgt ccaaacagc aaacattgt gaagatgta 
      tacgatttg atgaaaaaa ctgtttgaa aatagtgcc 
      actaaacca aaaattgcc ctcatgaca tatccagtc 
      caccacatt aacagcgtt gatatcgtg attacaatc 
      cccagcact cttacacat tgccttaaa tctcggact 
      tttaatttg aaacgaatg tgtcacttg gtgttagaa 
      gacgcgagt tgtttacta aaaaacaat cacgaagaa 
      atttacaaa attttggaa gtcactgat gaaatgctt 
      gaacacagg atgtgtagc accaaagtc caattcata 
      gtagcttca acaaaatgg acatcggat ttgcaaaaa 
      ttactcaaa tcgatttat gtaacacca cttgtttgc 
      atagggaat tatttggaa gcagctcta tatgggaac 
      atccaattc gaagtaaag tttttagtg tcacaaaac 
      aagaaacac tacattgaa ggattatta agagacaag 
      agtcatctt tttaaaact attgttgtg tgccataat 
      aatgaaata agcgatttg aataaatgg ttggttcga 
      aacggcatc gaagtcatg gctttaaat gatgattcg 
      actaaggaa caaatcgag gtttgtgag gaagaatgg 
      gcttttatg agaggtggc caatacaaa gtgttactg 
      tgcacggat tttattttc gactcatta ttgtgcatc 
      accgacgcc atgtggctc gtcagttac tcattttcc 
      gcaacttgg acccgattt atcaaacgt ttcagcgct 
      ttgtcctca cattacaag agtccttgg gaaacaaat 
      gaagaagaa gtcaaaccc aaatgtttt ttgatgatt 
      gacgaaaac tgcgaacaa caaatgaca aaagtcctc 
      aatttactc aaccgcttg agcaaggat ttgcctcca 
      agttaccaa aaatacgcc agtgcgttc aagagtaaa 
      actgaaaat gagaaaatc gacaatggg attgagatt 
      tgtaacaca ttgaaactg tttgggaaa tgtggcgat 
      tccttgtgt gagaagcga catttcgtt tgtgaacaa 
      ctggacgtt tcggaaaat ctgccaaag agtggaaaa 
      ctcgtattt aagattctt aatattaaa gatgtgaca 
      cagttttcc gtccaggta attagacac atcgatttg 
      gaggggaaa gtgaaggaa attaatgat gacgatttg 
      agaaaaccc ctcacagtt tcagaaaaa cgtgccaaa 
      aacattaaa gtgggaaaa ttttacgca cattgtgac 
      tacacccct gaaggtgat gtgttcatg ttgtgtcac 
      gtgttgagc atccccaac gataatttc gttgaagtc 
      aaatttgca aatgagttg gttttgcaa actttacag 
      agaaaactg tacgagctt ccgccaaat ttagagatc 
      gtggatgtc attatgtgc aatcttatt ccccccttc 
      aaagatgaa atgttttca gcgcgatct tttcacgtc 
      actcacaat tccctacaa actgtcgaa tataaaaat 
      atgatttta accggtgac atctatctg caattggat 
      cgaactttc tggttgaat aacatcgct caagtttta 
      caagtcgat ggttgcgac atcattaaa tttaatcta 
      actaagcat ttaacgaat ttaaaagtc gtcgaatta 
      tcttcgaat caaatcacc agcctgcac aaactatgt 
      gagaaatgt ggcataacc ctcccttgc tacctcaaa 
      cacactgca aaaaaggcc tcgaaagca aaagaagtc 
      gaagcgcaa cgagccttc ttagacgat aatgtccac 
      gaagtgatc ctcagctca gccgtcagt cctgaggag 
      ttctacgtc cgattggcc aaatttcaa ccactgttg 
      gagaacttg gaaagggac attcaggag agcatcagt 
      aagggaatc cccgagaag catttgaac atcgaaatc 
      ggaaagtac tacctggcc ttggactct cactacgag 
      ttgtattcg cgcgtcctc attttaaat atccaagat 
      tcgaaggcg ctttgtttt tacgtggat tacggtgac 
      gaagccata gtcgagctt tcgaaactg aaaatgttg 
      ccatcgaaa ttcctaatt cgattgccg tttcaagcg 
      attcagtgt cgcttgtat gggttgagc cccatttcg 
      ggcgaatgg gacgatgaa gccactgat gttctatac 
      aagtacatg tttgaaccc cagtcagat gttttccgg 
      tcaatatac gtacaaacg ttatgtaag gatgcttcc 
      gagacgatg agagttaaa acgacttat tccgtcttg 
      atgaaggac gggtttggc gataacaac gttttgatc 
      aaccagttg atgttggat tgtgggttt gcgacgtcg 
      atttttgac aaaattgat gattttgag ataccgaga 
      ccggaggcc gtggaggag gaatcgtcg gattcggat 
      gctgggtgt ttggaggtg ggtccagaa gaacctaat 
      cttgaagaa atttttgga gacgatttt gatttggaa 
      ataattgac gccgaggaa tttctcgaa gttttgggg 
      gttgcacgt cgtgaaaat ccctcctta gctttagaa 
      aatagtgat tcaaatcac actgaaacc attcatgat 
      ccgatcaat tctcaaatt gaccagact gttgagaaa 
      agtaattgt ctcacgccg gacgtttat tggtcacaa 
      accgaaaac accgtcaag cttaaaatc aagctaatc 
      ggtgtcgaa aattacaaa acgaacgtg gttcagaaa 
      cgaattttt acatttagt tcgcttctc aatgagaaa 
      aattacgag ctgaagttt acgttacac aaaccggtc 
      aagatggca aatcatcgg gcgttgggg caagaagtt 
      gtggttgtt ttgaataaa ttggagcct gtggagtgg 
      ttacagttg acgggcact caaagacgc atgaggttt 
      gttcattac gaagtgcct gagtttaaa gaagaagcc 
      aaacgaaag ctcctccag cttgatatt acggacgat 
      gagagcgac gaaaatgac ggggaagca ccgatgtat 
      catgtgata tccgacatg gatagcgag tttgatgaa 
      gagatcgat tcgagtgag tccgatcta tag
    mRNA sequence
    • >TC031042 RA
      cctagaatt aaataattt ttgttgtgt tttgttgtg 
      gtgtgtttt tgtgaattt gcacacgtt tttgcccta 
      cttaaggcc tttcagtcg caatggcca ccagggaga 
      aagaccctt tcgacagcg acgagcaaa aacactccg 
      atagtgatg gagccccaa agcagactt ttgagcccg 
      aaatcgaca atccagaga tgttcatga aaccggcgt 
      gtgagtgcc agaactgcg tccattgga cccaaagcg 
      ccgactgga caacgccgg atttggagt tcgaggggc 
      accacaaac gtgtttgca aagagcgga agattcaac 
      ccttgttac gatcggcac gacatccag ggaaaaccc 
      ctaagatct tcactttca atcctggca cattgacaa 
      aaattgaga aacaaagct acttgaaga ccctagttc 
      acggggagg tgatccccc agcctgtaa agttgctaa 
      cagacacct actttagcg acgaaattc aagctgctt 
      tgggtcgcc ttaactaca agtatagtt taccaatcc 
      aatcattcg tctggcccg caattttca gacaattga 
      atgttgtta tggtagggg ggcctaagt cggggaaaa 
      ccatgtcgt atctcccgg ccctttgta ccttcagta 
      cagctgaag aggaaaaat atcgacaat tatcaaagt 
      acaagggcc cgttagtgg ttattattt gtccaaaca 
      gcaaacatt gtgaagatg tatacgatt tgatgaaaa 
      aactgtttg aaaatagtg ccactaaac caaaaattg 
      ccctcatga catatccag tccaccaca ttaacagcg 
      ttgatatcg tgattacaa tccccagca ctcttacac 
      attgcctta aatctcgga cttttaatt tgaaacgaa 
      tgtgtcact tggtgttag aagacgcga gttgtttac 
      taaaaaaca atcacgaag aaatttaca aaattttgg 
      aagtcactg atgaaatgc ttgaacaca ggatgtgta 
      gcaccaaag tccaattca tagtagctt caacaaaat 
      ggacatcgg atttgcaaa aattactca aatcgattt 
      atgtaacac cacttgttt gcataggga attatttgg 
      aagcagctc tatatggga acatccaat tcgaagtaa 
      agtttttag tgtcacaaa acaagaaac actacattg 
      aaggattat taagagaca agagtcatc tttttaaaa 
      ctattgttg tgtgccata ataatgaaa taagcgatt 
      tgaataaat ggttggttc gaaacggca tcgaagtca 
      tggctttaa atgatgatt cgactaagg aacaaatcg 
      aggtttgtg aggaagaat gggctttta tgagaggtg 
      gccaataca aagtgttac tgtgcacgg attttattt 
      tcgactcat tattgtgca tcaccgacg ccatgtggc 
      tcgtcagtt actcatttt ccgcaactt ggacccgat 
      ttatcaaac gtttcagcg ctttgtcct cacattaca 
      agagtcctt gggaaacaa atgaagaag aagtcaaac 
      ccaaatgtt ttttgatga ttgacgaaa actgcgaac 
      aacaaatga caaaagtcc tcaatttac tcaaccgct 
      tgagcaagg atttgcctc caagttacc aaaaatacg 
      ccagtgcgt tcaagagta aaactgaaa atgagaaaa 
      tcgacaatg ggattgaga tttgtaaca cattgaaac 
      tgtttggga aatgtggcg attccttgt gtgagaagc 
      gacatttcg tttgtgaac aactggacg tttcggaaa 
      atctgccaa agagtggaa aactcgtat ttaagattc 
      ttaatatta aagatgtga cacagtttt ccgtccagg 
      taattagac acatcgatt tggagggga aagtgaagg 
      aaattaatg atgacgatt tgagaaaac ccctcacag 
      tttcagaaa aacgtgcca aaaacatta aagtgggaa 
      aattttacg cacattgtg actacaccc ctgaaggtg 
      atgtgttca tgttgtgtc acgtgttga gcatcccca 
      acgataatt tcgttgaag tcaaatttg caaatgagt 
      tggttttgc aaactttac agagaaaac tgtacgagc 
      ttccgccaa atttagaga tcgtggatg tcattatgt 
      gcaatctta ttcccccct tcaaagatg aaatgtttt 
      cagcgcgat cttttcacg tcactcaca attccctac 
      aaactgtcg aatataaaa atatgattt taaccggtg 
      acatctatc tgcaattgg atcgaactt tctggttga 
      ataacatcg ctcaagttt tacaagtcg atggttgcg 
      acatcatta aatttaatc taactaagc atttaacga 
      atttaaaag tcgtcgaat tatcttcga atcaaatca 
      ccagcctgc acaaactat gtgagaaat gtggcataa 
      ccctccctt gctacctca aacacactg caaaaaagg 
      cctcgaaag caaaagaag tcgaagcgc aacgagcct 
      tcttagacg ataatgtcc acgaagtga tcctcagct 
      cagccgtca gtcctgagg agttctacg tccgattgg 
      ccaaatttc aaccactgt tggagaact tggaaaggg 
      acattcagg agagcatca gtaagggaa tccccgaga 
      agcatttga acatcgaaa tcggaaagt actacctgg 
      ccttggact ctcactacg agttgtatt cgcgcgtcc 
      tcattttaa atatccaag attcgaagg cgctttgtt 
      tttacgtgg attacggtg acgaagcca tagtcgagc 
      tttcgaaac tgaaaatgt tgccatcga aattcctaa 
      ttcgattgc cgtttcaag cgattcagt gtcgcttgt 
      atgggttga gccccattt cgggcgaat gggacgatg 
      aagccactg atgttctat acaagtaca tgtttgaac 
      cccagtcag atgttttcc ggtcaatat acgtacaaa 
      cgttatgta aggatgctt ccgagacga tgagagtta 
      aaacgactt attccgtct tgatgaagg acgggtttg 
      gcgataaca acgttttga tcaaccagt tgatgttgg 
      attgtgggt ttgcgacgt cgatttttg acaaaattg 
      atgattttg agataccga gaccggagg ccgtggagg 
      aggaatcgt cggattcgg atgctgggt gtttggagg 
      tgggtccag aagaaccta atcttgaag aaatttttg 
      gagacgatt ttgatttgg aaataattg acgccgagg 
      aatttctcg aagttttgg gggttgcac gtcgtgaaa 
      atccctcct tagctttag aaaatagtg attcaaatc 
      acactgaaa ccattcatg atccgatca attctcaaa 
      ttgaccaga ctgttgaga aaagtaatt gtctcacgc 
      cggacgttt attggtcac aaaccgaaa acaccgtca 
      agcttaaaa tcaagctaa tcggtgtcg aaaattaca 
      aaacgaacg tggttcaga aacgaattt ttacattta 
      gttcgcttc tcaatgaga aaaattacg agctgaagt 
      ttacgttac acaaaccgg tcaagatgg caaatcatc 
      gggcgttgg ggcaagaag ttgtggttg ttttgaata 
      aattggagc ctgtggagt ggttacagt tgacgggca 
      ctcaaagac gcatgaggt ttgttcatt acgaagtgc 
      ctgagttta aagaagaag ccaaacgaa agctcctcc 
      agcttgata ttacggacg atgagagcg acgaaaatg 
      acggggaag caccgatgt atcatgtga tatccgaca 
      tggatagcg agtttgatg aagagatcg attcgagtg 
      agtccgatc tataggtct ataattgat tttttgaca 
      ttattatta ccaaagttt atttcttgt tttattatg 
      accaaactt taatttttt taataaatt cgacgattt 
      gat