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Gene information
General information
TC000107 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 6522013..6531668
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000107
    iBeetle screen iB_02705
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 75.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    urogomphi distal pigmentation/tanning darker - Number of animals showing the phenotype: 6

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000107 RA
      MANSIVEWY NGKSVFVTG GSGFMGKVL IEKLLFSCT 
      GIKEIYVLM RSKKGKSPE TRIQEMWKL PMFKRLRES 
      QPGAIKKVI PVIGDLNTE GLGLSPHDL NLLVTNANV 
      VFHCGATLK LEASLKDAI EQNTAGTAR VIDVSKKIK 
      NLYVFVYYS TAFCSADID VFEEKVYDC RDNPRDVIE 
      VSRWMKNDA LDPVTKSII APHPNTYTY SKRLAEKLV 
      ADELENMKV CIIRPSVVT PAVKEPLPG WVDSLNGPM 
      GLLVGAGKG VIRSMHVKA ENRAQTVPV DIAINATIV 
      IAQRIGSTT EKLKEVPVY NLTQDEVVP YTMGEILDI 
      GRRIVYENP FEMQIWYPD GDIRSSRLI HNIYCIFLH 
      WLPAYFIDF LLFLFRYKT FMVRLQRKI HDGLELLQF 
      FTVRQWVFR SSNFLALSK SMTDEENKT FPMDFKAVP 
      VEEYLTTAV LGARQYLMK EDLSTIPRC RTKQKILYV 
      VHKIFVYGV YLLLIWMLV STIPHLKVM FDAAGSYAH 
      KLPIVGTLI TK
    CDS sequence
    • >TC000107 RA
      atggctaat tcgatagtg gagtggtat aatggaaaa 
      tcggttttt gtaaccggc ggaagcggt ttcatggga 
      aaagtgctc atcgagaag ctgctgttt tcatgcact 
      ggaatcaaa gaaatctac gttttgatg cgctccaaa 
      aaaggaaaa agtccagag acacgcatt caagaaatg 
      tggaaattg ccgatgttt aagcgtctg agagagtcc 
      caacctggg gctataaag aaggtgatc cccgtcatt 
      ggtgacttg aacaccgaa ggcttgggt ttgagcccc 
      catgatttg aatttgctt gtcaccaat gccaatgtg 
      gtgtttcac tgtggggcc acccttaaa cttgaagcc 
      tcccttaaa gacgccatt gaacaaaac accgcaggg 
      acagctcgt gttattgat gtgtccaaa aaaatcaaa 
      aatttgtac gttttcgtg tattacagt actgcgttt 
      tgttcggcc gatatcgac gtgtttgaa gaaaaggtc 
      tatgactgc cgtgacaac ccccgcgac gtcatcgaa 
      gtctcccgc tggatgaaa aacgacgct ttggacccc 
      gtcaccaaa tccataata gccccccat cccaacacc 
      tacacttac agcaaacga ctcgccgaa aaactagtc 
      gccgacgaa ctcgaaaat atgaaagtt tgcatcata 
      cgaccatca gttgtcacg ccggccgtc aaggaacct 
      ctaccagga tgggtcgac agtttaaac ggcccaatg 
      gggctgtta gtgggcgct ggaaagggc gtaatacgc 
      tcaatgcac gtcaaggcc gaaaatagg gcacaaaca 
      gtccctgtg gacattgcc atcaatgcc acgatcgtc 
      atcgcgcaa aggatcggc tccacgacg gaaaagtta 
      aaagaggtc ccggtgtac aatttgacc caagacgaa 
      gtcgtgcct tacaccatg ggcgagatt ctggatatt 
      ggccgtcga attgtctac gaaaatccg tttgaaatg 
      caaatttgg taccccgat ggtgacatt cgctcctcg 
      cgtttaatt cacaacatt tattgcatt tttttgcac 
      tggttaccg gcgtacttt atcgacttt cttctcttt 
      ctcttccga tacaagaca ttcatggtg cgattacag 
      agaaaaatc cacgacggt ttagaactt ttacaattt 
      ttcacagtc cgccagtgg gttttccga agttcgaat 
      tttctcgct ttgagcaaa agcatgaca gatgaggag 
      aataagacg tttccgatg gactttaaa gctgttcct 
      gtggaggag tatttgaca actgcggtg cttggagca 
      aggcaatat ttgatgaaa gaggattta tcgacgatt 
      ccgcgatgc agaacgaag cagaaaatt ttgtacgtt 
      gtacataaa atatttgtt tacggtgtt tatctgctt 
      ctaatatgg atgcttgtg tcaacaata ccgcatttg 
      aaagtcatg tttgacgct gctggaagc tatgctcac 
      aaactacca attgttggg acacttatt accaagtaa
    mRNA sequence
    • >TC000107 RA
      tacattttg aaagtctac ctttgatat gctgtaata 
      acccagatg aacttagtc ttgtttatg ctctagtgg 
      tgtcggttg ctcgttttg ttggaaaaa taacgaaaa 
      cacgacttt gttcttcac atagcttgc ggctataac 
      agtcggaaa tcatggcta attcgatag tggagtggt 
      ataatggaa aatcggttt ttgtaaccg gcggaagcg 
      gtttcatgg gaaaagtgc tcatcgaga agctgctgt 
      tttcatgca ctggaatca aagaaatct acgttttga 
      tgcgctcca aaaaaggaa aaagtccag agacacgca 
      ttcaagaaa tgtggaaat tgccgatgt ttaagcgtc 
      tgagagagt cccaacctg gggctataa agaaggtga 
      tccccgtca ttggtgact tgaacaccg aaggcttgg 
      gtttgagcc cccatgatt tgaatttgc ttgtcacca 
      atgccaatg tggtgtttc actgtgggg ccaccctta 
      aacttgaag cctccctta aagacgcca ttgaacaaa 
      acaccgcag ggacagctc gtgttattg atgtgtcca 
      aaaaaatca aaaatttgt acgttttcg tgtattaca 
      gtactgcgt tttgttcgg ccgatatcg acgtgtttg 
      aagaaaagg tctatgact gccgtgaca acccccgcg 
      acgtcatcg aagtctccc gctggatga aaaacgacg 
      ctttggacc ccgtcacca aatccataa tagcccccc 
      atcccaaca cctacactt acagcaaac gactcgccg 
      aaaaactag tcgccgacg aactcgaaa atatgaaag 
      tttgcatca tacgaccat cagttgtca cgccggccg 
      tcaaggaac ctctaccag gatgggtcg acagtttaa 
      acggcccaa tggggctgt tagtgggcg ctggaaagg 
      gcgtaatac gctcaatgc acgtcaagg ccgaaaata 
      gggcacaaa cagtccctg tggacattg ccatcaatg 
      ccacgatcg tcatcgcgc aaaggatcg gctccacga 
      cggaaaagt taaaagagg tcccggtgt acaatttga 
      cccaagacg aagtcgtgc cttacacca tgggcgaga 
      ttctggata ttggccgtc gaattgtct acgaaaatc 
      cgtttgaaa tgcaaattt ggtaccccg atggtgaca 
      ttcgctcct cgcgtttaa ttcacaaca tttattgca 
      tttttttgc actggttac cggcgtact ttatcgact 
      ttcttctct ttctcttcc gatacaaga cattcatgg 
      tgcgattac agagaaaaa tccacgacg gtttagaac 
      ttttacaat ttttcacag tccgccagt gggttttcc 
      gaagttcga attttctcg ctttgagca aaagcatga 
      cagatgagg agaataaga cgtttccga tggacttta 
      aagctgttc ctgtggagg agtatttga caactgcgg 
      tgcttggag caaggcaat atttgatga aagaggatt 
      tatcgacga ttccgcgat gcagaacga agcagaaaa 
      ttttgtacg ttgtacata aaatatttg tttacggtg 
      tttatctgc ttctaatat ggatgcttg tgtcaacaa 
      taccgcatt tgaaagtca tgtttgacg ctgctggaa 
      gctatgctc acaaactac caattgttg ggacactta 
      ttaccaagt aaagcagtg gtttttgct ggttttagg 
      ggattcttg cacctttat tgttatatt ttgtgtatt 
      ttataaaag aggaataaa tgttaaaaa taaatattt 
      ctttaacta cgtgtaata aagatgatt atgatca