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Gene information
General information
TC000100 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11211007..11240056
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000100
    iBeetle screen iB_02704

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    elytra slightly partiallyblistered - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000100 RA
      MATEVENNA PPAAAETKE IKEETTPAA DAKAEEAKQ 
      EAGGDAPKD KEPAPPKVL VHKSNYEKD VVYLYQFSR 
      TPLLPSLSP FCLKVESWL RLAGIKYEN VDHKMKYRS 
      KKGQLPFVE INGEEIADS TIIIKELSQ KFGHDLDAA 
      LTPEQRNIA HASISMVEN HLAWVVMWW RTKYPELVL 
      KGYKVNLQH ALGTRIPNG ILNFFFKFA FGRKGTKKV 
      KAQGIGVHS ADEINAFGQ GDLKVLSDM LADKPFFFG 
      DEPTTLDVV VFAQLAQIY FIDKEVSYA LRDYMTESC 
      PNLVGLVNR MKERCFPDW DDICSTLDL NSHLPKPEE 
      AKDNKEGGK DEKEGDQEK EPDDKDIEK EKADEKEKD 
      KGKEKETEE NKEKETK
    CDS sequence
    • >TC000100 RA
      atggccacc gaagtcgag aacaacgcg ccgcccgcc 
      gccgccgag accaaggag atcaaggag gagacgacg 
      ccggccgcc gacgccaaa gccgaggag gcgaagcag 
      gaagccggc ggcgacgcc cccaaggac aaggagccg 
      gcgcccccc aaagtgcta gtgcacaaa tccaactac 
      gaaaaagat gtcgtttat ctttaccag ttttcacga 
      acgccgctt ctgccatcc ttgtcgccc ttctgcctc 
      aaagtcgag tcttggctg agactggct ggcattaaa 
      tacgagaat gtggaccat aaaatgaaa taccgctca 
      aaaaaaggc caactgccc ttcgtggag ataaacggc 
      gaggagatc gccgacagt acaatcatc atcaaagag 
      ctttcgcaa aagttcgga cacgatctc gatgctgct 
      cttactccc gaacaaagg aatatcgct cacgcctcc 
      atctccatg gttgagaat cacctcgca tgggtggtg 
      atgtggtgg cgcaccaaa tatccagaa ctagtgctc 
      aaaggatat aaagttaat ttgcaacat gctctcggc 
      acgaggata cccaacgga attctcaac ttctttttc 
      aaattcgcc ttcgggagg aagggtacc aagaaagta 
      aaggctcag ggtatagga gtgcattcg gccgacgaa 
      atcaacgct ttcggccaa ggagatctc aaagtgctc 
      tctgacatg ttagcggac aaacctttc ttcttcggc 
      gacgaacca acaactctg gatgtagtc gtatttgca 
      caactagca caaatttac tttatcgat aaggaagtt 
      agttatgca ttgcgcgat tatatgaca gagtcgtgt 
      ccgaatctc gttggtttg gtgaatcgc atgaaggag 
      agatgtttc cccgattgg gacgatatt tgcagcacg 
      ttggacttg aactcgcat ttgccaaaa ccggaagag 
      gccaaggat aacaaagaa ggaggcaaa gacgagaag 
      gaaggagac caggagaag gaacccgac gataaagat 
      atcgaaaaa gaaaaggct gacgagaag gagaaagac 
      aagggcaag gaaaaagaa acggaggaa aacaaagag 
      aaagaaaca aaataa
    mRNA sequence
    • >TC000100 RA
      tcgcacaag ggtggggcc cccatgcat gtgcatggc 
      aacaacgcc gttggggtg ggggttgag cgtcgcttg 
      ctcgcttcg cttgccgaa gagtgacgt cacgacccg 
      cccgctaac tcttcttgt caggttcgc tggagcaac 
      actcgagtg tacgtcgtt gttgtgttg gtagcagtt 
      gtgtgtttg tgttcttca aataatcaa atataatgg 
      ccaccgaag tcgagaaca acgcgccgc ccgccgccg 
      ccgagacca aggagatca aggaggaga cgacgccgg 
      ccgccgacg ccaaagccg aggaggcga agcaggaag 
      ccggcggcg acgccccca aggacaagg agccggcgc 
      cccccaaag tgctagtgc acaaatcca actacgaaa 
      aagatgtcg tttatcttt accagtttt cacgaacgc 
      cgcttctgc catccttgt cgcccttct gcctcaaag 
      tcgagtctt ggctgagac tggctggca ttaaatacg 
      agaatgtgg accataaaa tgaaatacc gctcaaaaa 
      aaggccaac tgcccttcg tggagataa acggcgagg 
      agatcgccg acagtacaa tcatcatca aagagcttt 
      cgcaaaagt tcggacacg atctcgatg ctgctctta 
      ctcccgaac aaaggaata tcgctcacg cctccatct 
      ccatggttg agaatcacc tcgcatggg tggtgatgt 
      ggtggcgca ccaaatatc cagaactag tgctcaaag 
      gatataaag ttaatttgc aacatgctc tcggcacga 
      ggataccca acggaattc tcaacttct ttttcaaat 
      tcgccttcg ggaggaagg gtaccaaga aagtaaagg 
      ctcagggta taggagtgc attcggccg acgaaatca 
      acgctttcg gccaaggag atctcaaag tgctctctg 
      acatgttag cggacaaac ctttcttct tcggcgacg 
      aaccaacaa ctctggatg tagtcgtat ttgcacaac 
      tagcacaaa tttacttta tcgataagg aagttagtt 
      atgcattgc gcgattata tgacagagt cgtgtccga 
      atctcgttg gtttggtga atcgcatga aggagagat 
      gtttccccg attgggacg atatttgca gcacgttgg 
      acttgaact cgcatttgc caaaaccgg aagaggcca 
      aggataaca aagaaggag gcaaagacg agaaggaag 
      gagaccagg agaaggaac ccgacgata aagatatcg 
      aaaaagaaa aggctgacg agaaggaga aagacaagg 
      gcaaggaaa aagaaacgg aggaaaaca aagagaaag 
      aaacaaaat aaagagact gagtctcca taagcccac 
      tgccgaggc aagtatccc ctccttacc ctcctcact 
      taacccttt gactctctt ttcatttaa atctcgact 
      acgtgcaac catctagtt ttaagactc attcatatt 
      gtaacactt tttaatgaa aatatgaaa ttatactct 
      tgtcaacga tttatttat gtagttttt tttatctat 
      tatagagga cagttttat caataaaat atattcatt 
      gctagttaa gaatacttg tttgtataa ataatttta 
      gctaggttt tttattgta agcaattgt atgtctgaa 
      aaactttgg gaaacattg catggattt taaacacga 
      tgacgatat gccatgctt cttaaagtt tatccatgt 
      tagtgcagt cttaatgta agaagtata acccaatta 
      gtagtgcat ttatattca ttgtattca ttccacatt 
      ttaacattg catcacaaa attaacata gcttgaccg 
      tatctattg tagattttt gtatttttt tattggatc 
      atttttaag ataacgaaa aattaaaaa caaactttt 
      gacaaattg tatatggat tgcagtgta tttctgaag 
      ctcaataga ttgactagc atgttaaaa atatatttt 
      agattttat tataagttt atttgaact tacaactgt 
      gttgtatgc tatattaca atttaaata aataaattg 
      gtattttaa gttgcttat ttgagtatg atgttgtaa 
      attttactg gacgtctga aaaaaaaaa cataaacgg 
      actgacaga ttcaatcat tttctttaa cccagtcat 
      cttccacat tttcccaaa atagaaaca actaactac 
      attagtgaa agggcagag cactctcga gtggcttta 
      ttagattaa gaaacaagt aactaacag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY