Status
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Gene information
General information
TC034503
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000083
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 11506329..11516313
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034503
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02703

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    procephalic head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034503 RA
      MSFVDKAAN QLVDYKNNS KNDSAATTG HGTPIGEKD 
      ATLTVGPRG PILLQDNNF IEELAHFDR ERIPERVVH 
      AKGAGAFGY FEVTHDITK YTAAKVFSN IGKRTPIAI 
      RFSTVAGES GSADTVRDP RGFAMKFYT EDGIWDLVG 
      NNTPIFFIR DPILFPHFI HSQKRNPQT HLKDANMFW 
      DFITLRPET THQTIHMNG YGSHTFKLV NAEGKAVYC 
      KFHHKTDQG IRNLDVQKA AQLASTDPD YGLRDLYNA 
      IANGNYPSW TTYIQVMTM EQAAACKFN PFDLTKVWP 
      HGEYPLIPV GKFTLNRNP ENYFAEVEQ IAFSPAHMI 
      PGIEPSPDK MLLGRLFSY GDTHRHRLG ANYLQLPVN 
      CPFKVRNYQ RDGPQAINN QGGAPNYHP NSFNGPDGD 
      SRAKALNVP FAVSGDANR YDSGDEDNY SQSRVFYQK 
      TLDQGARDR LIANIVGNL KDAADFIQE RAIRNFTQV 
      DAGLGQKIA EGLKKQKRA NL
    CDS sequence
    • >TC034503 RA
      atgtcgttt gtagataaa gccgctaat cagctcgtg 
      gattataag aacaattca aagaatgac tccgccgcc 
      accaccggc cacggcacc ccaatcggc gagaaagac 
      gccaccctc accgtaggc ccccgcggc cccatcctc 
      ctccaagac aacaacttc atcgaagag ctcgcccac 
      ttcgaccgg gagcgcatc cccgaacgc gtcgtccat 
      gccaagggg gccggagcc ttcggctac ttcgaagtc 
      acccacgac atcaccaaa tacaccgcc gccaaagtc 
      ttctccaac atcggcaag cgcaccccc atcgccatc 
      cgcttctca acagtggct ggcgagtcg ggctccgcc 
      gacaccgtc cgcgacccc cgcggcttc gccatgaag 
      ttctacacc gaagacggc atctgggac ctggtgggc 
      aacaacacg cccatattc ttcatccgc gaccccatc 
      ctcttcccc cacttcata cacagccag aaacgcaat 
      cctcagacg cacctaaaa gacgccaac atgttctgg 
      gattttatc accttgagg ccggagacg acccatcag 
      accatacac atgaatggt tatgggtcg cacactttc 
      aagctggtg aacgctgag ggtaaagcc gtgtattgc 
      aaattccac cacaagact gaccagggg attaggaat 
      ctggacgtg cagaaagca gcacagttg gcttcgact 
      gacccagat tatgggttg agagatttg tacaatgcc 
      atcgccaat ggtaattac ccatcatgg accacctac 
      attcaagtc atgaccatg gagcaagcc gccgcttgc 
      aaattcaat ccatttgat ttgaccaaa gtttggccc 
      catggcgag taccctctc attcccgtg ggcaaattt 
      acgctcaat cgcaacccg gagaactac ttcgcagag 
      gtggagcaa atcgcgttt agtccagcc catatgatc 
      ccaggtatt gaaccatca cccgataaa atgctcctg 
      gggaggctc ttctcctac ggggacacg caccgtcac 
      cgtttgggg gccaattac ctccaactc cccgtcaac 
      tgtcccttc aaagtgcgc aattatcag cgtgacggc 
      ccccaggcg attaacaat caagggggc gcccccaac 
      taccacccc aatagtttc aacgggcct gatggggac 
      tcgagggca aaggctttg aacgttccg tttgctgtg 
      tcaggagac gccaatcgt tacgattct ggcgatgag 
      gataactac agccagtca agggtgttt taccagaag 
      actttggat cagggggct cgggacagg ttgattgcg 
      aatattgtc gggaatttg aaagacgcc gccgatttt 
      atccaagaa agggctatc aggaatttc actcaagtg 
      gatgctggg ttgggacag aagatcgcc gaagggttg 
      aagaagcaa aaacgggct aatttgtaa
    mRNA sequence
    • >TC034503 RA
      aaactcaaa aaataattg tagtagcgc catctacat 
      ttcacttgt ttcttcttg gccaataga aatggatgt 
      tccgccact ggtgcccac gtgatctct aggtcgtac 
      agttcatat cacgttgat ataaacaga tgttttcaa 
      cgtaatttt atgcatttt tgctgctgc tgctgcgtt 
      gttgtgttg tttactggt gttttttct tgtaatttc 
      tggacaaaa tgtcgtttg tagataaag ccgctaatc 
      agctcgtgg attataaga acaattcaa agaatgact 
      ccgccgcca ccaccggcc acggcaccc caatcggcg 
      agaaagacg ccaccctca ccgtaggcc cccgcggcc 
      ccatcctcc tccaagaca acaacttca tcgaagagc 
      tcgcccact tcgaccggg agcgcatcc ccgaacgcg 
      tcgtccatg ccaaggggg ccggagcct tcggctact 
      tcgaagtca cccacgaca tcaccaaat acaccgccg 
      ccaaagtct tctccaaca tcggcaagc gcaccccca 
      tcgccatcc gcttctcaa cagtggctg gcgagtcgg 
      gctccgccg acaccgtcc gcgaccccc gcggcttcg 
      ccatgaagt tctacaccg aagacggca tctgggacc 
      tggtgggca acaacacgc ccatattct tcatccgcg 
      accccatcc tcttccccc acttcatac acagccaga 
      aacgcaatc ctcagacgc acctaaaag acgccaaca 
      tgttctggg attttatca ccttgaggc cggagacga 
      cccatcaga ccatacaca tgaatggtt atgggtcgc 
      acactttca agctggtga acgctgagg gtaaagccg 
      tgtattgca aattccacc acaagactg accagggga 
      ttaggaatc tggacgtgc agaaagcag cacagttgg 
      cttcgactg acccagatt atgggttga gagatttgt 
      acaatgcca tcgccaatg gtaattacc catcatgga 
      ccacctaca ttcaagtca tgaccatgg agcaagccg 
      ccgcttgca aattcaatc catttgatt tgaccaaag 
      tttggcccc atggcgagt accctctca ttcccgtgg 
      gcaaattta cgctcaatc gcaacccgg agaactact 
      tcgcagagg tggagcaaa tcgcgttta gtccagccc 
      atatgatcc caggtattg aaccatcac ccgataaaa 
      tgctcctgg ggaggctct tctcctacg gggacacgc 
      accgtcacc gtttggggg ccaattacc tccaactcc 
      ccgtcaact gtcccttca aagtgcgca attatcagc 
      gtgacggcc cccaggcga ttaacaatc aagggggcg 
      cccccaact accacccca atagtttca acgggcctg 
      atggggact cgagggcaa aggctttga acgttccgt 
      ttgctgtgt caggagacg ccaatcgtt acgattctg 
      gcgatgagg ataactaca gccagtcaa gggtgtttt 
      accagaaga ctttggatc agggggctc gggacaggt 
      tgattgcga atattgtcg ggaatttga aagacgccg 
      ccgatttta tccaagaaa gggctatca ggaatttca 
      ctcaagtgg atgctgggt tgggacaga agatcgccg 
      aagggttga agaagcaaa aacgggcta atttgtaat 
      ttttcattt attttatga cgttacata actatatgt 
      agactattt gtgcttgtc atgggttat ctcaagtat 
      tttattttg tcactggtg agtgtttca gcgatacgc 
      tgatggttg aagttttat aaaaccaat aaagaccat 
      taaactgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY