| General information | |||
| TC003645 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
scaffold_seqid16: 5807427..5823448 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003645 | |
| General information | ||||
| iB_02702 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
- >TC003645 RA
MMHTPVGTL ERETIPPPY SPSIRDDLS VMSHVITRK DGCNNLTNS VVRLQNELN NFSLHKSAS EPTMVATTI PQPQQDSSP DTPQPAPYD LNKGWMTGI FGCLRPVLS IIGKGAVSE IKNSHQDDW EIPFEQITD LTYLGSGGQ GTVFSGMLN NQKVAVKKV YDIKETDIR NLKKLNHPN IVKFKGVCT QLPCLSIIM EYCPYGPLF NLLKNQKNV VTINRVVSW AKQITSGMH YLHSQKIIH RDLKSPNVL IGEEEVIKI SDFGTSRTW SGVSEKMSF AGTVAWMAP EAIKELECS EKVDIWSFG VVLWELLTC EVPYDGMEQ SAIMYMVGC GKLRPPIPK TCPDGFRLI MEMCWKLNP KERPSFKLI LNHLQIASV EILGKYEDN QFFQTQESW KEEIKSQIT QFKAQLQKH RIEFQLKEE QLIKKRENE IKHVRDIRE LYDRKLEKV HQLYLELSA VLQQVEQYN RQGGRKRDI QKNRRLINP FVRKMERRR SNHSTTPTS PECSLTSPD SPQTTPVKA PLFAKLNTA TNNPESVTQ SSQKFRKRH YRTNSGSPR NSKSSNRSS SVVDAETQT DSMDLSETE LSPSFDLPV CITSKRVPQ RVVLHELKA ESDDEGCNG NVQLRYVSD SHDTEVEVF SRRHSESLI YDQEDTLNQ NDATRREFS DEDNLETLG RKVSALKNG NIFTDIQNS AENGNVTDL DVLRKNCAC SAQDSTEDA CNDSFTDEE GEVYNNTLW RKSSARRPI YPVRRSNRT KSSLNFHQK QQLNGSDEG DTSEYSVSP SSKSSTLES NPDRAKAAA LKRPNEKCS SDSESDQNV TIVTQVSYK SESVV
- >TC003645 RA
atgatgcat actcctgtt ggaaccttg gagcgggag accatccct cctccctat tctccatca atcagagac gatctgagt gttatgtcc catgtcata acacgtaaa gatgggtgc aacaatctc accaacagt gtcgttcgg ttacaaaat gagttgaac aacttcagc ctccacaaa agtgcgagc gaaccaacg atggttgca acaaccatc ccccagccc caacaagac agctctccc gacaccccc caaccagcc ccttacgat ctgaacaag ggctggatg accgggatt tttgggtgt ctgcggccc gttttgtcg atcattggc aaaggggca gtcagcgag attaaaaac agtcatcaa gatgattgg gaaattccg tttgaacaa atcactgat ttgacctat ttgggttcg ggtggtcaa gggaccgtt ttttccgga atgttgaac aaccaaaaa gtggctgtt aaaaaagtg tacgacatc aaagagacc gatattagg aatttgaaa aagttgaat caccctaac attgttaag tttaaagga gtatgcact caacttcct tgcctcagt attataatg gagtattgt ccatatggg cctctcttc aacttactc aaaaaccag aagaatgtt gtcacaatc aaccgagtg gtttcctgg gccaaacaa ataacaagc ggaatgcat tatcttcac tcacagaag attattcac cgagatttg aaaagtccc aatgtttta attggggaa gaagaagtg atcaagatt agcgatttt ggtacttca cggacttgg agtggagtt agcgaaaaa atgagtttt gctggaacc gtcgcttgg atggccccc gaagccatt aaagagttg gaatgttcg gaaaaagtc gacatttgg tcgtttggt gtcgtttta tgggaatta ctcacgtgt gaagtccca tacgatggg atggaacaa agcgccatc atgtacatg gtcggttgt gggaaactg agacctccc atcccgaaa acctgccca gatgggttc cgactcatc atggaaatg tgctggaaa ctcaacccc aaagaacgg ccctcgttc aaactaatc ctgaaccat ttacaaatt gcctcggtt gagattttg ggtaaatac gaggataat cagttcttc caaactcag gagagctgg aaggaagag atcaagtcc cagataacg cagtttaag gcccagttg cagaaacac cgcattgaa ttccaactg aaggaagaa cagttgatc aaaaaacgc gagaatgag attaaacac gttcgcgat attcgggaa ctgtacgac cggaagctg gaaaaagtg caccagttg taccttgag ttatcggct gttttgcag caagttgaa caatataac aggcaaggg gggcgcaag cgcgatatt cagaaaaat cgccgtttg atcaacccc tttgtccgc aaaatggaa cggaggcga agcaatcac agcacgacg cctacgagc cccgagtgc agtctcacg agccccgac agcccccaa actactcca gtgaaagcc ccgcttttt gccaaactg aacaccgcc actaataat ccggaatcg gtgacccag tcgagtcaa aagttccgg aagagacac taccggacc aattccggg tcgcctcgc aattcgaaa agttccaac cggagttcg agtgttgtg gatgcggag actcagacg gattcgatg gatttgagt gagactgag cttagtccg agttttgat cttccggtg tgcattaca tcgaagagg gtgcctcag agggttgtt cttcatgag ttgaaggcc gagtcggat gacgaaggg tgcaatggg aatgtgcag ttgagatat gtgagcgat agccatgac acagaggtt gaggtgttc agccgacgc cactcggag tcgctcatt tacgaccag gaggacact ctcaatcag aacgacgcc acccgaagg gaattcagc gacgaagac aatctcgaa acgctcggc cgcaaagtt tcggcgttg aaaaacggc aacatcttc actgatata caaaattcg gccgaaaat ggcaacgtc accgacttg gacgtcttg cggaaaaac tgcgcttgc agtgcacaa gacagcacc gaagatgcg tgcaatgac agttttacc gatgaagaa ggcgaagtc tacaataac acgttgtgg agaaagagc tcggcgcga cgccccatc taccccgtc cgccgctcg aaccgtacc aaatccagc ctgaatttc caccagaag cagcagctg aacggctcc gacgaaggc gacacctcc gaatactca gtctcaccg tccagcaaa agctccaca cttgaatcg aacccggat cgggcgaaa gccgccgcc ctcaagcgc cccaacgag aaatgttca tctgatagc gaatcagat caaaacgta acaatcgtt actcaagta tcctacaag tcagagagt gtagtctga
- >TC003645 RA
tctgaagcg aaaacaata aatcagttt ttaatcaga acggcgaaa atgtacgcc attaaacat tccaaacaa ataataagc ttcagatgt ttaattttc ctcatttta agcaattgg cgtacaaac aaatgattt aatgcaaac gagtaggta tttcgtgca ataaattga taagactca tatctttga cgatgacgt cttatttaa agcgtcaca ccattgcaa aacaaagtg atgaactga attcaagac aaacaatag atagggaaa gtaatttaa atatcaaat agaggaagt atcgatttt ttatatttt ttccggaga caaatttat ttccaagat tgtctaatt atccttcgc aaaattttc caaataaat acgagaaaa ctggaacaa atttatttg cgagatctc caagccaaa attgcgtct cgagttccg cgaatttct ccgagcttt tctcggaaa cattcatta ctctaaatg tcaaagttc cttattgca tcacaggat aacgcacgc ccctggaaa aatcaatgc tgaaactat tctccaggc atttattcc ggtcgcaaa atggcgcgt aaacgcttg atattacta ccgattccg gtgattacg gtattttaa ttgtgatat aatagagtt gatcaaagc ccaggacca ccggaataa atggataga tgatgcata ctcctgttg gaaccttgg agcgggaga ccatccctc ctccctatt ctccatcaa tcagagacg atctgagtg ttatgtccc atgtcataa cacgtaaag atgggtgca acaatctca ccaacagtg tcgttcggt tacaaaatg agttgaaca acttcagcc tccacaaaa gtgcgagcg aaccaacga tggttgcaa caaccatcc cccagcccc aacaagaca gctctcccg acacccccc aaccagccc cttacgatc tgaacaagg gctggatga ccgggattt ttgggtgtc tgcggcccg ttttgtcga tcattggca aaggggcag tcagcgaga ttaaaaaca gtcatcaag atgattggg aaattccgt ttgaacaaa tcactgatt tgacctatt tgggttcgg gtggtcaag ggaccgttt tttccggaa tgttgaaca accaaaaag tggctgtta aaaaagtgt acgacatca aagagaccg atattagga atttgaaaa agttgaatc accctaaca ttgttaagt ttaaaggag tatgcactc aacttcctt gcctcagta ttataatgg agtattgtc catatgggc ctctcttca acttactca aaaaccaga agaatgttg tcacaatca accgagtgg tttcctggg ccaaacaaa taacaagcg gaatgcatt atcttcact cacagaaga ttattcacc gagatttga aaagtccca atgttttaa ttggggaag aagaagtga tcaagatta gcgattttg gtacttcac ggacttgga gtggagtta gcgaaaaaa tgagttttg ctggaaccg tcgcttgga tggcccccg aagccatta aagagttgg aatgttcgg aaaaagtcg acatttggt cgtttggtg tcgttttat gggaattac tcacgtgtg aagtcccat acgatggga tggaacaaa gcgccatca tgtacatgg tcggttgtg ggaaactga gacctccca tcccgaaaa cctgcccag atgggttcc gactcatca tggaaatgt gctggaaac tcaacccca aagaacggc cctcgttca aactaatcc tgaaccatt tacaaattg cctcggttg agattttgg gtaaatacg aggataatc agttcttcc aaactcagg agagctgga aggaagaga tcaagtccc agataacgc agtttaagg cccagttgc agaaacacc gcattgaat tccaactga aggaagaac agttgatca aaaaacgcg agaatgaga ttaaacacg ttcgcgata ttcgggaac tgtacgacc ggaagctgg aaaaagtgc accagttgt accttgagt tatcggctg ttttgcagc aagttgaac aatataaca ggcaagggg ggcgcaagc gcgatattc agaaaaatc gccgtttga tcaacccct ttgtccgca aaatggaac ggaggcgaa gcaatcaca gcacgacgc ctacgagcc ccgagtgca gtctcacga gccccgaca gcccccaaa ctactccag tgaaagccc cgctttttg ccaaactga acaccgcca ctaataatc cggaatcgg tgacccagt cgagtcaaa agttccgga agagacact accggacca attccgggt cgcctcgca attcgaaaa gttccaacc ggagttcga gtgttgtgg atgcggaga ctcagacgg attcgatgg atttgagtg agactgagc ttagtccga gttttgatc ttccggtgt gcattacat cgaagaggg tgcctcaga gggttgttc ttcatgagt tgaaggccg agtcggatg acgaagggt gcaatggga atgtgcagt tgagatatg tgagcgata gccatgaca cagaggttg aggtgttca gccgacgcc actcggagt cgctcattt acgaccagg aggacactc tcaatcaga acgacgcca cccgaaggg aattcagcg acgaagaca atctcgaaa cgctcggcc gcaaagttt cggcgttga aaaacggca acatcttca ctgatatac aaaattcgg ccgaaaatg gcaacgtca ccgacttgg acgtcttgc ggaaaaact gcgcttgca gtgcacaag acagcaccg aagatgcgt gcaatgaca gttttaccg atgaagaag gcgaagtct acaataaca cgttgtgga gaaagagct cggcgcgac gccccatct accccgtcc gccgctcga accgtacca aatccagcc tgaatttcc accagaagc agcagctga acggctccg acgaaggcg acacctccg aatactcag tctcaccgt ccagcaaaa gctccacac ttgaatcga acccggatc gggcgaaag ccgccgccc tcaagcgcc ccaacgaga aatgttcat ctgatagcg aatcagatc aaaacgtaa caatcgtta ctcaagtat cctacaagt cagagagtg tagtctgaa taggtcatt gtgatttaa ttagttaat tttaagtat ccacgtatt tttttacct gattgatgt tgtttgttt taaggaact ctgtgcaat ttttagtaa tttattcag ttgccgtct attttttac aagtattat gcaccaagg aaaagtcgt gacaatttt cgggaaaac ttgccactt tacagccaa ctttcctct ctatttatt tgcacttga aagtattag aatactcat cgtgactca gttattgac aaaattttt attatgtat gtggttgta gttataagt attttcaca actaaacta tgtggctaa atatttaag cggcaataa cttcgaacg ttcaagctt ctaaagata aagtacaat gtggaattc ggaatatta tagcaaaac tataccgct agctgggtc tattttata gcaagtatt gtagatgta aagatatat ttattgata cttttaaga gctaaagtt ctgctataa ttttcatag caaatccgt tgccttgta gacacatcg atttttttc cattccggc caatttaac acttgaaag tgttaattt tttattgga gcagcatat tattaaccg gttgtgtgt tacatgtaa gtacttaca tccgtttcg cgtccgtta ttaccttaa ttttcatgt gatacaaaa acattccta attattttg taaagatca aaacttttg ttgtgattt agttttacg aaaaatcat ctcaactgc cagacattc ctctatttt attttgtac atcgttcat tagttgaat aaaagtgtc gacaattga aatggtgtt tcactcccc tttgaggta cggttaccg tggctcctg gcgacctta aatagtaat aaaaaaatt gggcgagca atttaatgg agtttataa caccgaaat ttagtgtgc ggccaaatt ggccaaaaa ttatagaca gccaatgtc ggagatcaa agcctgagc gtttaaaag gttggccag gtgcagttg cagtccttc gtcgccaag aaaatgtcc agatgaact gtttagatt gtttagtct acccacatc agctctaat tacctgcat ctaaaaact ataattgcg ctcggctct agcaaacgc aaatttatc gcagagggc tgggtccac ttgtctcga cgcatccac aatattatg tataacgga gttttgaga aatgacttg ttattttta agaggcttt gtgtggaaa acgcgctga aaggtccag tagtcggat tacattaca aaggccggc ttcaggtga gttggcgaa ctttgaccc aaggaccat tgtaccatc atattgcta atttatcac atggcggcg ggtctactg gactgaccc ggcatacat tacacttgt gctgggttg tgggaaaaa taaaactca acacgattc