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Gene information
General information
TC003645 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 10368714..10384735
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003645
    iBeetle screen iB_02702

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003645 RA
      MMHTPVGTL ERETIPPPY SPSIRDDLS VMSHVITRK 
      DGCNNLTNS VVRLQNELN NFSLHKSAS EPTMVATTI 
      PQPQQDSSP DTPQPAPYD LNKGWMTGI FGCLRPVLS 
      IIGKGAVSE IKNSHQDDW EIPFEQITD LTYLGSGGQ 
      GTVFSGMLN NQKVAVKKV YDIKETDIR NLKKLNHPN 
      IVKFKGVCT QLPCLSIIM EYCPYGPLF NLLKNQKNV 
      VTINRVVSW AKQITSGMH YLHSQKIIH RDLKSPNVL 
      IGEEEVIKI SDFGTSRTW SGVSEKMSF AGTVAWMAP 
      EAIKELECS EKVDIWSFG VVLWELLTC EVPYDGMEQ 
      SAIMYMVGC GKLRPPIPK TCPDGFRLI MEMCWKLNP 
      KERPSFKLI LNHLQIASV EILGKYEDN QFFQTQESW 
      KEEIKSQIT QFKAQLQKH RIEFQLKEE QLIKKRENE 
      IKHVRDIRE LYDRKLEKV HQLYLELSA VLQQVEQYN 
      RQGGRKRDI QKNRRLINP FVRKMERRR SNHSTTPTS 
      PECSLTSPD SPQTTPVKA PLFAKLNTA TNNPESVTQ 
      SSQKFRKRH YRTNSGSPR NSKSSNRSS SVVDAETQT 
      DSMDLSETE LSPSFDLPV CITSKRVPQ RVVLHELKA 
      ESDDEGCNG NVQLRYVSD SHDTEVEVF SRRHSESLI 
      YDQEDTLNQ NDATRREFS DEDNLETLG RKVSALKNG 
      NIFTDIQNS AENGNVTDL DVLRKNCAC SAQDSTEDA 
      CNDSFTDEE GEVYNNTLW RKSSARRPI YPVRRSNRT 
      KSSLNFHQK QQLNGSDEG DTSEYSVSP SSKSSTLES 
      NPDRAKAAA LKRPNEKCS SDSESDQNV TIVTQVSYK 
      SESVV
    CDS sequence
    • >TC003645 RA
      atgatgcat actcctgtt ggaaccttg gagcgggag 
      accatccct cctccctat tctccatca atcagagac 
      gatctgagt gttatgtcc catgtcata acacgtaaa 
      gatgggtgc aacaatctc accaacagt gtcgttcgg 
      ttacaaaat gagttgaac aacttcagc ctccacaaa 
      agtgcgagc gaaccaacg atggttgca acaaccatc 
      ccccagccc caacaagac agctctccc gacaccccc 
      caaccagcc ccttacgat ctgaacaag ggctggatg 
      accgggatt tttgggtgt ctgcggccc gttttgtcg 
      atcattggc aaaggggca gtcagcgag attaaaaac 
      agtcatcaa gatgattgg gaaattccg tttgaacaa 
      atcactgat ttgacctat ttgggttcg ggtggtcaa 
      gggaccgtt ttttccgga atgttgaac aaccaaaaa 
      gtggctgtt aaaaaagtg tacgacatc aaagagacc 
      gatattagg aatttgaaa aagttgaat caccctaac 
      attgttaag tttaaagga gtatgcact caacttcct 
      tgcctcagt attataatg gagtattgt ccatatggg 
      cctctcttc aacttactc aaaaaccag aagaatgtt 
      gtcacaatc aaccgagtg gtttcctgg gccaaacaa 
      ataacaagc ggaatgcat tatcttcac tcacagaag 
      attattcac cgagatttg aaaagtccc aatgtttta 
      attggggaa gaagaagtg atcaagatt agcgatttt 
      ggtacttca cggacttgg agtggagtt agcgaaaaa 
      atgagtttt gctggaacc gtcgcttgg atggccccc 
      gaagccatt aaagagttg gaatgttcg gaaaaagtc 
      gacatttgg tcgtttggt gtcgtttta tgggaatta 
      ctcacgtgt gaagtccca tacgatggg atggaacaa 
      agcgccatc atgtacatg gtcggttgt gggaaactg 
      agacctccc atcccgaaa acctgccca gatgggttc 
      cgactcatc atggaaatg tgctggaaa ctcaacccc 
      aaagaacgg ccctcgttc aaactaatc ctgaaccat 
      ttacaaatt gcctcggtt gagattttg ggtaaatac 
      gaggataat cagttcttc caaactcag gagagctgg 
      aaggaagag atcaagtcc cagataacg cagtttaag 
      gcccagttg cagaaacac cgcattgaa ttccaactg 
      aaggaagaa cagttgatc aaaaaacgc gagaatgag 
      attaaacac gttcgcgat attcgggaa ctgtacgac 
      cggaagctg gaaaaagtg caccagttg taccttgag 
      ttatcggct gttttgcag caagttgaa caatataac 
      aggcaaggg gggcgcaag cgcgatatt cagaaaaat 
      cgccgtttg atcaacccc tttgtccgc aaaatggaa 
      cggaggcga agcaatcac agcacgacg cctacgagc 
      cccgagtgc agtctcacg agccccgac agcccccaa 
      actactcca gtgaaagcc ccgcttttt gccaaactg 
      aacaccgcc actaataat ccggaatcg gtgacccag 
      tcgagtcaa aagttccgg aagagacac taccggacc 
      aattccggg tcgcctcgc aattcgaaa agttccaac 
      cggagttcg agtgttgtg gatgcggag actcagacg 
      gattcgatg gatttgagt gagactgag cttagtccg 
      agttttgat cttccggtg tgcattaca tcgaagagg 
      gtgcctcag agggttgtt cttcatgag ttgaaggcc 
      gagtcggat gacgaaggg tgcaatggg aatgtgcag 
      ttgagatat gtgagcgat agccatgac acagaggtt 
      gaggtgttc agccgacgc cactcggag tcgctcatt 
      tacgaccag gaggacact ctcaatcag aacgacgcc 
      acccgaagg gaattcagc gacgaagac aatctcgaa 
      acgctcggc cgcaaagtt tcggcgttg aaaaacggc 
      aacatcttc actgatata caaaattcg gccgaaaat 
      ggcaacgtc accgacttg gacgtcttg cggaaaaac 
      tgcgcttgc agtgcacaa gacagcacc gaagatgcg 
      tgcaatgac agttttacc gatgaagaa ggcgaagtc 
      tacaataac acgttgtgg agaaagagc tcggcgcga 
      cgccccatc taccccgtc cgccgctcg aaccgtacc 
      aaatccagc ctgaatttc caccagaag cagcagctg 
      aacggctcc gacgaaggc gacacctcc gaatactca 
      gtctcaccg tccagcaaa agctccaca cttgaatcg 
      aacccggat cgggcgaaa gccgccgcc ctcaagcgc 
      cccaacgag aaatgttca tctgatagc gaatcagat 
      caaaacgta acaatcgtt actcaagta tcctacaag 
      tcagagagt gtagtctga
    mRNA sequence
    • >TC003645 RA
      tctgaagcg aaaacaata aatcagttt ttaatcaga 
      acggcgaaa atgtacgcc attaaacat tccaaacaa 
      ataataagc ttcagatgt ttaattttc ctcatttta 
      agcaattgg cgtacaaac aaatgattt aatgcaaac 
      gagtaggta tttcgtgca ataaattga taagactca 
      tatctttga cgatgacgt cttatttaa agcgtcaca 
      ccattgcaa aacaaagtg atgaactga attcaagac 
      aaacaatag atagggaaa gtaatttaa atatcaaat 
      agaggaagt atcgatttt ttatatttt ttccggaga 
      caaatttat ttccaagat tgtctaatt atccttcgc 
      aaaattttc caaataaat acgagaaaa ctggaacaa 
      atttatttg cgagatctc caagccaaa attgcgtct 
      cgagttccg cgaatttct ccgagcttt tctcggaaa 
      cattcatta ctctaaatg tcaaagttc cttattgca 
      tcacaggat aacgcacgc ccctggaaa aatcaatgc 
      tgaaactat tctccaggc atttattcc ggtcgcaaa 
      atggcgcgt aaacgcttg atattacta ccgattccg 
      gtgattacg gtattttaa ttgtgatat aatagagtt 
      gatcaaagc ccaggacca ccggaataa atggataga 
      tgatgcata ctcctgttg gaaccttgg agcgggaga 
      ccatccctc ctccctatt ctccatcaa tcagagacg 
      atctgagtg ttatgtccc atgtcataa cacgtaaag 
      atgggtgca acaatctca ccaacagtg tcgttcggt 
      tacaaaatg agttgaaca acttcagcc tccacaaaa 
      gtgcgagcg aaccaacga tggttgcaa caaccatcc 
      cccagcccc aacaagaca gctctcccg acacccccc 
      aaccagccc cttacgatc tgaacaagg gctggatga 
      ccgggattt ttgggtgtc tgcggcccg ttttgtcga 
      tcattggca aaggggcag tcagcgaga ttaaaaaca 
      gtcatcaag atgattggg aaattccgt ttgaacaaa 
      tcactgatt tgacctatt tgggttcgg gtggtcaag 
      ggaccgttt tttccggaa tgttgaaca accaaaaag 
      tggctgtta aaaaagtgt acgacatca aagagaccg 
      atattagga atttgaaaa agttgaatc accctaaca 
      ttgttaagt ttaaaggag tatgcactc aacttcctt 
      gcctcagta ttataatgg agtattgtc catatgggc 
      ctctcttca acttactca aaaaccaga agaatgttg 
      tcacaatca accgagtgg tttcctggg ccaaacaaa 
      taacaagcg gaatgcatt atcttcact cacagaaga 
      ttattcacc gagatttga aaagtccca atgttttaa 
      ttggggaag aagaagtga tcaagatta gcgattttg 
      gtacttcac ggacttgga gtggagtta gcgaaaaaa 
      tgagttttg ctggaaccg tcgcttgga tggcccccg 
      aagccatta aagagttgg aatgttcgg aaaaagtcg 
      acatttggt cgtttggtg tcgttttat gggaattac 
      tcacgtgtg aagtcccat acgatggga tggaacaaa 
      gcgccatca tgtacatgg tcggttgtg ggaaactga 
      gacctccca tcccgaaaa cctgcccag atgggttcc 
      gactcatca tggaaatgt gctggaaac tcaacccca 
      aagaacggc cctcgttca aactaatcc tgaaccatt 
      tacaaattg cctcggttg agattttgg gtaaatacg 
      aggataatc agttcttcc aaactcagg agagctgga 
      aggaagaga tcaagtccc agataacgc agtttaagg 
      cccagttgc agaaacacc gcattgaat tccaactga 
      aggaagaac agttgatca aaaaacgcg agaatgaga 
      ttaaacacg ttcgcgata ttcgggaac tgtacgacc 
      ggaagctgg aaaaagtgc accagttgt accttgagt 
      tatcggctg ttttgcagc aagttgaac aatataaca 
      ggcaagggg ggcgcaagc gcgatattc agaaaaatc 
      gccgtttga tcaacccct ttgtccgca aaatggaac 
      ggaggcgaa gcaatcaca gcacgacgc ctacgagcc 
      ccgagtgca gtctcacga gccccgaca gcccccaaa 
      ctactccag tgaaagccc cgctttttg ccaaactga 
      acaccgcca ctaataatc cggaatcgg tgacccagt 
      cgagtcaaa agttccgga agagacact accggacca 
      attccgggt cgcctcgca attcgaaaa gttccaacc 
      ggagttcga gtgttgtgg atgcggaga ctcagacgg 
      attcgatgg atttgagtg agactgagc ttagtccga 
      gttttgatc ttccggtgt gcattacat cgaagaggg 
      tgcctcaga gggttgttc ttcatgagt tgaaggccg 
      agtcggatg acgaagggt gcaatggga atgtgcagt 
      tgagatatg tgagcgata gccatgaca cagaggttg 
      aggtgttca gccgacgcc actcggagt cgctcattt 
      acgaccagg aggacactc tcaatcaga acgacgcca 
      cccgaaggg aattcagcg acgaagaca atctcgaaa 
      cgctcggcc gcaaagttt cggcgttga aaaacggca 
      acatcttca ctgatatac aaaattcgg ccgaaaatg 
      gcaacgtca ccgacttgg acgtcttgc ggaaaaact 
      gcgcttgca gtgcacaag acagcaccg aagatgcgt 
      gcaatgaca gttttaccg atgaagaag gcgaagtct 
      acaataaca cgttgtgga gaaagagct cggcgcgac 
      gccccatct accccgtcc gccgctcga accgtacca 
      aatccagcc tgaatttcc accagaagc agcagctga 
      acggctccg acgaaggcg acacctccg aatactcag 
      tctcaccgt ccagcaaaa gctccacac ttgaatcga 
      acccggatc gggcgaaag ccgccgccc tcaagcgcc 
      ccaacgaga aatgttcat ctgatagcg aatcagatc 
      aaaacgtaa caatcgtta ctcaagtat cctacaagt 
      cagagagtg tagtctgaa taggtcatt gtgatttaa 
      ttagttaat tttaagtat ccacgtatt tttttacct 
      gattgatgt tgtttgttt taaggaact ctgtgcaat 
      ttttagtaa tttattcag ttgccgtct attttttac 
      aagtattat gcaccaagg aaaagtcgt gacaatttt 
      cgggaaaac ttgccactt tacagccaa ctttcctct 
      ctatttatt tgcacttga aagtattag aatactcat 
      cgtgactca gttattgac aaaattttt attatgtat 
      gtggttgta gttataagt attttcaca actaaacta 
      tgtggctaa atatttaag cggcaataa cttcgaacg 
      ttcaagctt ctaaagata aagtacaat gtggaattc 
      ggaatatta tagcaaaac tataccgct agctgggtc 
      tattttata gcaagtatt gtagatgta aagatatat 
      ttattgata cttttaaga gctaaagtt ctgctataa 
      ttttcatag caaatccgt tgccttgta gacacatcg 
      atttttttc cattccggc caatttaac acttgaaag 
      tgttaattt tttattgga gcagcatat tattaaccg 
      gttgtgtgt tacatgtaa gtacttaca tccgtttcg 
      cgtccgtta ttaccttaa ttttcatgt gatacaaaa 
      acattccta attattttg taaagatca aaacttttg 
      ttgtgattt agttttacg aaaaatcat ctcaactgc 
      cagacattc ctctatttt attttgtac atcgttcat 
      tagttgaat aaaagtgtc gacaattga aatggtgtt 
      tcactcccc tttgaggta cggttaccg tggctcctg 
      gcgacctta aatagtaat aaaaaaatt gggcgagca 
      atttaatgg agtttataa caccgaaat ttagtgtgc 
      ggccaaatt ggccaaaaa ttatagaca gccaatgtc 
      ggagatcaa agcctgagc gtttaaaag gttggccag 
      gtgcagttg cagtccttc gtcgccaag aaaatgtcc 
      agatgaact gtttagatt gtttagtct acccacatc 
      agctctaat tacctgcat ctaaaaact ataattgcg 
      ctcggctct agcaaacgc aaatttatc gcagagggc 
      tgggtccac ttgtctcga cgcatccac aatattatg 
      tataacgga gttttgaga aatgacttg ttattttta 
      agaggcttt gtgtggaaa acgcgctga aaggtccag 
      tagtcggat tacattaca aaggccggc ttcaggtga 
      gttggcgaa ctttgaccc aaggaccat tgtaccatc 
      atattgcta atttatcac atggcggcg ggtctactg 
      gactgaccc ggcatacat tacacttgt gctgggttg 
      tgggaaaaa taaaactca acacgattc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY