| General information | |||
| TC030754 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 9286134..9315581 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC030754 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02701 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC030754 RA
MWSMKCQPR RPPFSVLVI LFGLFNFRN CQEIKCGHP AIPLNARVS LSSPGLAPG TVAKYQCDE GYETFGNTE TSCSPAGKW IGELPFCGT NIAYRKPAN QSTTVRGGS ALNANDGEK STEHDGKRC SETQKEASP WWHVDLLQP YPVKVVRVT TRGCCGHQP LQDLEIRVG NSSTDLQRN PLCAWFPGT IDEGVTKTF TCARVLTGQ YVFLQLVGV EGSLSLCEV EVFTTDEFS VDRCAPRGA PEDVQLASF ARTCYEFGV SRGGSFQEA RNYCRSHNG DLVHGMSAA ATTFLYAEL ERRKPMLKT QLVWIGAQK EPGITSRVW KWVNGDLVQ RPAWGKDQP NNYNGEQNC VVLDGGRGW LWNDVGCNL DYLPWICQH SPSSCGSPD KQINTTIKG SNYNVGSRI EYQCPEGHM LVGEATRLC GKEGFWSGK APTCKYVSC EALPGLEHG SVVLKDKRT TYGAQALYT CHENYTLIG HEIRTCGSN GTWTNSTPK CLFDWCPDP PSINGGIVK TSGRRAGDT AVYSCQSGF ILFGQGVLS CELGGQWSG KAPSCKFVD CGAPPNIDN GRYDLLNGT TTVESLVEY SCGHDYWLE GQKVQKCTR EGKWSADAP SCELITCEE PDVPAGSYV VGYDFNVHS SIEYHCEVG HVLRGEPIH TCTKEGDWS GTTPTCEFV DCGKVPTML YGTVNYIND TTYLDSQIE YSCAKNYRL NGVSRRRCL ENKQWSNTP PKCEEIRCP EPILAEHSI LSVTGNDRM YGRTLIRTA ESSSTTYKI GAIVKYRCE RGYKVVGEP LSTCEDTGQ WSGDVPQCV FVDCGNPEN IQNGKVTLT SNATYYGAL ALYSCDKNF ELDGVSRRL CLENGTWSS DAPTCRGIQ CKEPDRFDG MTVKVSTYS VGGVVAYSC PRGHTMQGN ATRVCLLKG VWSGRAPTC TAVDCKHPG TIENGRVIV MNGTTYNAA IEYHCVPNF ERVGPYLRK CMENGEWSG EVPRCEEIT GEPQEPSSL GTNIGIGAG VVVFLLILL LVIYLKLRK PIPVKNTEN VEGAERKED RNAAVMSYA TLSDRNGHL PPNIYENIH DENMYDAPY EETSRDSGT YEPEPISWK NGNMVTING VSVR
- >TC030754 RA
atgtggtca atgaaatgt cagccaaga aggcctcca tttagtgtt ttagtgatc ttgtttgga ttattcaac ttcaggaac tgtcaagaa atcaaatgt gggcatcct gccattccc ttgaacgcc agggtctcc ctgagctct cccggactc gctccggga accgtggcc aaataccag tgcgatgag ggatatgag acgtttggt aacacggaa acttcgtgt tcgccggct ggaaaatgg atcggcgag ttgccgttt tgtggcacc aacatcgct taccgcaaa cccgccaac caatcaacc accgttcgc gggggcagc gccctgaac gccaacgac ggcgaaaaa agcaccgaa cacgacgga aaacgctgc tccgagacc cagaaggag gccagcccc tggtggcac gtcgacctc ctccagccc taccccgtg aaggtggtg cgggtgacc accaggggc tgctgcggc caccagccc ctccaggac ctggagatc cgggtcgga aacagcagc actgacctg cagcggaac cctctctgc gcctggttt ccgggaaca atagacgaa ggcgtaacg aagacattc acctgtgca cgagtttta acaggacaa tacgttttc ctgcagctg gtgggagtc gaagggtcg ttgtcgctc tgcgaagtg gaggttttc acaacggac gagttttcc gtggatcgc tgcgccccg aggggggcc cctgaggac gtccagttg gcgtccttt gcccgcact tgctacgag ttcggggtt agccgcgga gggtcgttc caggaagcg cgcaattac tgcaggagc cataacggg gacctggtg cacggcatg agcgcagct gcaacgacg tttctttat gcggaactc gagaggcgg aagccgatg ctgaagaca caactggtt tggatcggt gcccaaaaa gagccagga atcacgtcg agggtctgg aaatgggtt aatggcgac ttggtccag cgtccagcg tggggaaaa gaccaacca aacaattat aacggtgaa caaaactgc gtagtcctc gacggtgga cgcgggtgg ttatggaat gatgtaggt tgcaatctc gactatcta ccatggatc tgtcaacac tctccatcg tcatgcggc agtcccgac aaacaaatt aacaccacc ataaaagga agcaattac aacgtcggc tcgaggatc gaataccaa tgtccagag gggcacatg ttggtcggt gaagcgacc agactttgc ggaaaggaa ggtttctgg tcggggaaa gcgccaacg tgcaaatat gtgagctgt gaggctttg ccgggcttg gaacatggt tccgtggtt ctaaaggat aaacggacg acatatggg gcacaggct ctttacacc tgtcacgaa aattacact cttataggg cacgaaata cggacttgt gggtctaat gggacttgg acgaattcc acgcctaaa tgtctcttc gactggtgt ccagatcca cctagtatt aatgggggg attgttaaa acgtcgggg cgaagggct ggagacacc gcggtttat tcgtgccag tctgggttt atcttgttt gggcaaggg gtgctttcg tgcgaactg gggggccag tggtcgggc aaagccccc tcgtgcaaa ttcgtggac tgtggggcc cctccaaat atcgacaat ggccggtac gacctgctc aatggtacc acaaccgtc gaaagtttg gtcgaatac agctgtggc catgactac tggctcgaa gggcaaaaa gtgcaaaaa tgcacaagg gagggcaaa tggtcagcg gacgcaccc tcatgtgaa ctgataacg tgcgaggaa cccgacgtt cctgcagga agttacgtg gttgggtac gattttaac gtacattcc tccattgag taccactgc gaggtggga catgtgttg cgaggggaa cccatccat acgtgtacc aaggaaggg gactggtct ggtaccact ccaacttgt gaattcgtt gattgtggt aaagtaccg accatgctg tacggtact gttaattat attaatgat actacgtac cttgacagt caaattgag tattcgtgt gctaagaat tacaggctt aatggggtt tcaaggagg cgctgtttg gaaaacaag cagtggagc aatactcca ccaaagtgt gaagaaata agatgtcct gagccaatt ttggccgaa catagcata ctttcagtg actgggaac gatcgcatg tacggtcgt actcttatc aggacagct gaatcctcc tcaactact tacaaaatc ggggccata gtcaaatac aggtgtgaa agaggctac aaagttgtg ggcgagcct ttgagcact tgtgaagat acgggtcag tggagtggc gatgttcct caatgcgtc tttgtggat tgtggtaac ccggaaaat atccaaaac ggtaaagtt accttaacc tcgaacgca acgtactat ggagcactg gccctctac tcctgcgac aaaaacttc gaactggat ggagtttca cgccgattg tgtcttgaa aatggtacc tggagctcc gacgctcca acatgtcgc ggaatccag tgcaaagaa cccgacagg tttgacgga atgacggtc aaagttagt acttacagt gttggggga gttgttgcc tattcatgt ccccgtggg cacaccatg caggggaat gcaactaga gtgtgcttg ctgaagggg gtttggagc ggacgggcg cccacttgc accgcggtc gattgtaaa caccctggt actatcgaa aatgggcga gtgattgtg atgaatggt accacctat aatgcggcg attgagtac cattgtgtc ccaaatttc gaaagagtg gggccgtat ttgcgaaaa tgtatggag aatggggaa tggtcgggt gaggtacca cgatgtgag gagattacc ggggaacca caggaaccc tcgagtctg gggaccaat attggaatt ggggccgga gtggtcgtc tttttgcta attctctta ttagtaatt tatcttaaa ttgcgtaag ccaatccca gtgaagaac accgagaac gtggaaggc gccgagcgc aaagaagac cgaaacgcc gcagtcatg agctacgcc actctgagt gaccgaaac ggtcacttg ccaccaaat atctacgaa aacattcac gatgaaaac atgtacgat gccccctat gaggagaca agtcgcgac agtggcact tacgagccc gaacccatt tcgtggaag aatggaaat atggttacc ataaatggg gtttcagta agatga
- >TC030754 RA
aaatggcac aaaacagcg tgttcttcg cctgctgaa ctttcattc cagtattga ccgcggcgg ttaaagcat ggctcttgg ctagaaatg ttcgagtga agtgattag ttaattgtg gtttataaa tgtgttcga acgaattta gcaatgtgg tcaatgaaa tgtcagcca agaaggcct ccatttagt gttttagtg atcttgttt ggattattc aacttcagg aactgtcaa gaaatcaaa tgtgggcat cctgccatt cccttgaac gccagggtc tccctgagc tctcccgga ctcgctccg ggaaccgtg gccaaatac cagtgcgat gagggatat gagacgttt ggtaacacg gaaacttcg tgttcgccg gctggaaaa tggatcggc gagttgccg ttttgtggc accaacatc gcttaccgc aaacccgcc aaccaatca accaccgtt cgcgggggc agcgccctg aacgccaac gacggcgaa aaaagcacc gaacacgac ggaaaacgc tgctccgag acccagaag gaggccagc ccctggtgg cacgtcgac ctcctccag ccctacccc gtgaaggtg gtgcgggtg accaccagg ggctgctgc ggccaccag cccctccag gacctggag atccgggtc ggaaacagc agcactgac ctgcagcgg aaccctctc tgcgcctgg tttccggga acaatagac gaaggcgta acgaagaca ttcacctgt gcacgagtt ttaacagga caatacgtt ttcctgcag ctggtggga gtcgaaggg tcgttgtcg ctctgcgaa gtggaggtt ttcacaacg gacgagttt tccgtggat cgctgcgcc ccgaggggg gcccctgag gacgtccag ttggcgtcc tttgcccgc acttgctac gagttcggg gttagccgc ggagggtcg ttccaggaa gcgcgcaat tactgcagg agccataac ggggacctg gtgcacggc atgagcgca gctgcaacg acgtttctt tatgcggaa ctcgagagg cggaagccg atgctgaag acacaactg gtttggatc ggtgcccaa aaagagcca ggaatcacg tcgagggtc tggaaatgg gttaatggc gacttggtc cagcgtcca gcgtgggga aaagaccaa ccaaacaat tataacggt gaacaaaac tgcgtagtc ctcgacggt ggacgcggg tggttatgg aatgatgta ggttgcaat ctcgactat ctaccatgg atctgtcaa cactctcca tcgtcatgc ggcagtccc gacaaacaa attaacacc accataaaa ggaagcaat tacaacgtc ggctcgagg atcgaatac caatgtcca gaggggcac atgttggtc ggtgaagcg accagactt tgcggaaag gaaggtttc tggtcgggg aaagcgcca acgtgcaaa tatgtgagc tgtgaggct ttgccgggc ttggaacat ggttccgtg gttctaaag gataaacgg acgacatat ggggcacag gctctttac acctgtcac gaaaattac actcttata gggcacgaa atacggact tgtgggtct aatgggact tggacgaat tccacgcct aaatgtctc ttcgactgg tgtccagat ccacctagt attaatggg gggattgtt aaaacgtcg gggcgaagg gctggagac accgcggtt tattcgtgc cagtctggg tttatcttg tttgggcaa ggggtgctt tcgtgcgaa ctggggggc cagtggtcg ggcaaagcc ccctcgtgc aaattcgtg gactgtggg gcccctcca aatatcgac aatggccgg tacgacctg ctcaatggt accacaacc gtcgaaagt ttggtcgaa tacagctgt ggccatgac tactggctc gaagggcaa aaagtgcaa aaatgcaca agggagggc aaatggtca gcggacgca ccctcatgt gaactgata acgtgcgag gaacccgac gttcctgca ggaagttac gtggttggg tacgatttt aacgtacat tcctccatt gagtaccac tgcgaggtg ggacatgtg ttgcgaggg gaacccatc catacgtgt accaaggaa ggggactgg tctggtacc actccaact tgtgaattc gttgattgt ggtaaagta ccgaccatg ctgtacggt actgttaat tatattaat gatactacg taccttgac agtcaaatt gagtattcg tgtgctaag aattacagg cttaatggg gtttcaagg aggcgctgt ttggaaaac aagcagtgg agcaatact ccaccaaag tgtgaagaa ataagatgt cctgagcca attttggcc gaacatagc atactttca gtgactggg aacgatcgc atgtacggt cgtactctt atcaggaca gctgaatcc tcctcaact acttacaaa atcggggcc atagtcaaa tacaggtgt gaaagaggc tacaaagtt gtgggcgag cctttgagc acttgtgaa gatacgggt cagtggagt ggcgatgtt cctcaatgc gtctttgtg gattgtggt aacccggaa aatatccaa aacggtaaa gttacctta acctcgaac gcaacgtac tatggagca ctggccctc tactcctgc gacaaaaac ttcgaactg gatggagtt tcacgccga ttgtgtctt gaaaatggt acctggagc tccgacgct ccaacatgt cgcggaatc cagtgcaaa gaacccgac aggtttgac ggaatgacg gtcaaagtt agtacttac agtgttggg ggagttgtt gcctattca tgtccccgt gggcacacc atgcagggg aatgcaact agagtgtgc ttgctgaag ggggtttgg agcggacgg gcgcccact tgcaccgcg gtcgattgt aaacaccct ggtactatc gaaaatggg cgagtgatt gtgatgaat ggtaccacc tataatgcg gcgattgag taccattgt gtcccaaat ttcgaaaga gtggggccg tatttgcga aaatgtatg gagaatggg gaatggtcg ggtgaggta ccacgatgt gaggagatt accggggaa ccacaggaa ccctcgagt ctggggacc aatattgga attggggcc ggagtggtc gtctttttg ctaattctc ttattagta atttatctt aaattgcgt aagccaatc ccagtgaag aacaccgag aacgtggaa ggcgccgag cgcaaagaa gaccgaaac gccgcagtc atgagctac gccactctg agtgaccga aacggtcac ttgccacca aatatctac gaaaacatt cacgatgaa aacatgtac gatgccccc tatgaggag acaagtcgc gacagtggc acttacgag cccgaaccc atttcgtgg aagaatgga aatatggtt accataaat ggggtttca gtaagatga gatgaaaag aactgaacc aattactgc taccaaaat caacctaga tgttgctta tagagattc tattttttg tttttacta attgatttg taatttaag tcgtgtttg aatgcatta ttcagttat gcggaaatg ttgcgatcg agagatgga aatgtgatt taaattaaa aatcaattg ccttgttgt aaattatgt gtatgataa gctttgtcg taatgactg tgtgagagt gttgtgggg tccacttgt tgatgtaaa tactcattt tgtaataaa ataggttta tgtattgta gataatatc accaataaa agaaaatga cctca
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
