Status
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Gene information
General information
TC030754 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 9286134..9315581
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030754
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02701

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030754 RA
      MWSMKCQPR RPPFSVLVI LFGLFNFRN CQEIKCGHP 
      AIPLNARVS LSSPGLAPG TVAKYQCDE GYETFGNTE 
      TSCSPAGKW IGELPFCGT NIAYRKPAN QSTTVRGGS 
      ALNANDGEK STEHDGKRC SETQKEASP WWHVDLLQP 
      YPVKVVRVT TRGCCGHQP LQDLEIRVG NSSTDLQRN 
      PLCAWFPGT IDEGVTKTF TCARVLTGQ YVFLQLVGV 
      EGSLSLCEV EVFTTDEFS VDRCAPRGA PEDVQLASF 
      ARTCYEFGV SRGGSFQEA RNYCRSHNG DLVHGMSAA 
      ATTFLYAEL ERRKPMLKT QLVWIGAQK EPGITSRVW 
      KWVNGDLVQ RPAWGKDQP NNYNGEQNC VVLDGGRGW 
      LWNDVGCNL DYLPWICQH SPSSCGSPD KQINTTIKG 
      SNYNVGSRI EYQCPEGHM LVGEATRLC GKEGFWSGK 
      APTCKYVSC EALPGLEHG SVVLKDKRT TYGAQALYT 
      CHENYTLIG HEIRTCGSN GTWTNSTPK CLFDWCPDP 
      PSINGGIVK TSGRRAGDT AVYSCQSGF ILFGQGVLS 
      CELGGQWSG KAPSCKFVD CGAPPNIDN GRYDLLNGT 
      TTVESLVEY SCGHDYWLE GQKVQKCTR EGKWSADAP 
      SCELITCEE PDVPAGSYV VGYDFNVHS SIEYHCEVG 
      HVLRGEPIH TCTKEGDWS GTTPTCEFV DCGKVPTML 
      YGTVNYIND TTYLDSQIE YSCAKNYRL NGVSRRRCL 
      ENKQWSNTP PKCEEIRCP EPILAEHSI LSVTGNDRM 
      YGRTLIRTA ESSSTTYKI GAIVKYRCE RGYKVVGEP 
      LSTCEDTGQ WSGDVPQCV FVDCGNPEN IQNGKVTLT 
      SNATYYGAL ALYSCDKNF ELDGVSRRL CLENGTWSS 
      DAPTCRGIQ CKEPDRFDG MTVKVSTYS VGGVVAYSC 
      PRGHTMQGN ATRVCLLKG VWSGRAPTC TAVDCKHPG 
      TIENGRVIV MNGTTYNAA IEYHCVPNF ERVGPYLRK 
      CMENGEWSG EVPRCEEIT GEPQEPSSL GTNIGIGAG 
      VVVFLLILL LVIYLKLRK PIPVKNTEN VEGAERKED 
      RNAAVMSYA TLSDRNGHL PPNIYENIH DENMYDAPY 
      EETSRDSGT YEPEPISWK NGNMVTING VSVR
    CDS sequence
    • >TC030754 RA
      atgtggtca atgaaatgt cagccaaga aggcctcca 
      tttagtgtt ttagtgatc ttgtttgga ttattcaac 
      ttcaggaac tgtcaagaa atcaaatgt gggcatcct 
      gccattccc ttgaacgcc agggtctcc ctgagctct 
      cccggactc gctccggga accgtggcc aaataccag 
      tgcgatgag ggatatgag acgtttggt aacacggaa 
      acttcgtgt tcgccggct ggaaaatgg atcggcgag 
      ttgccgttt tgtggcacc aacatcgct taccgcaaa 
      cccgccaac caatcaacc accgttcgc gggggcagc 
      gccctgaac gccaacgac ggcgaaaaa agcaccgaa 
      cacgacgga aaacgctgc tccgagacc cagaaggag 
      gccagcccc tggtggcac gtcgacctc ctccagccc 
      taccccgtg aaggtggtg cgggtgacc accaggggc 
      tgctgcggc caccagccc ctccaggac ctggagatc 
      cgggtcgga aacagcagc actgacctg cagcggaac 
      cctctctgc gcctggttt ccgggaaca atagacgaa 
      ggcgtaacg aagacattc acctgtgca cgagtttta 
      acaggacaa tacgttttc ctgcagctg gtgggagtc 
      gaagggtcg ttgtcgctc tgcgaagtg gaggttttc 
      acaacggac gagttttcc gtggatcgc tgcgccccg 
      aggggggcc cctgaggac gtccagttg gcgtccttt 
      gcccgcact tgctacgag ttcggggtt agccgcgga 
      gggtcgttc caggaagcg cgcaattac tgcaggagc 
      cataacggg gacctggtg cacggcatg agcgcagct 
      gcaacgacg tttctttat gcggaactc gagaggcgg 
      aagccgatg ctgaagaca caactggtt tggatcggt 
      gcccaaaaa gagccagga atcacgtcg agggtctgg 
      aaatgggtt aatggcgac ttggtccag cgtccagcg 
      tggggaaaa gaccaacca aacaattat aacggtgaa 
      caaaactgc gtagtcctc gacggtgga cgcgggtgg 
      ttatggaat gatgtaggt tgcaatctc gactatcta 
      ccatggatc tgtcaacac tctccatcg tcatgcggc 
      agtcccgac aaacaaatt aacaccacc ataaaagga 
      agcaattac aacgtcggc tcgaggatc gaataccaa 
      tgtccagag gggcacatg ttggtcggt gaagcgacc 
      agactttgc ggaaaggaa ggtttctgg tcggggaaa 
      gcgccaacg tgcaaatat gtgagctgt gaggctttg 
      ccgggcttg gaacatggt tccgtggtt ctaaaggat 
      aaacggacg acatatggg gcacaggct ctttacacc 
      tgtcacgaa aattacact cttataggg cacgaaata 
      cggacttgt gggtctaat gggacttgg acgaattcc 
      acgcctaaa tgtctcttc gactggtgt ccagatcca 
      cctagtatt aatgggggg attgttaaa acgtcgggg 
      cgaagggct ggagacacc gcggtttat tcgtgccag 
      tctgggttt atcttgttt gggcaaggg gtgctttcg 
      tgcgaactg gggggccag tggtcgggc aaagccccc 
      tcgtgcaaa ttcgtggac tgtggggcc cctccaaat 
      atcgacaat ggccggtac gacctgctc aatggtacc 
      acaaccgtc gaaagtttg gtcgaatac agctgtggc 
      catgactac tggctcgaa gggcaaaaa gtgcaaaaa 
      tgcacaagg gagggcaaa tggtcagcg gacgcaccc 
      tcatgtgaa ctgataacg tgcgaggaa cccgacgtt 
      cctgcagga agttacgtg gttgggtac gattttaac 
      gtacattcc tccattgag taccactgc gaggtggga 
      catgtgttg cgaggggaa cccatccat acgtgtacc 
      aaggaaggg gactggtct ggtaccact ccaacttgt 
      gaattcgtt gattgtggt aaagtaccg accatgctg 
      tacggtact gttaattat attaatgat actacgtac 
      cttgacagt caaattgag tattcgtgt gctaagaat 
      tacaggctt aatggggtt tcaaggagg cgctgtttg 
      gaaaacaag cagtggagc aatactcca ccaaagtgt 
      gaagaaata agatgtcct gagccaatt ttggccgaa 
      catagcata ctttcagtg actgggaac gatcgcatg 
      tacggtcgt actcttatc aggacagct gaatcctcc 
      tcaactact tacaaaatc ggggccata gtcaaatac 
      aggtgtgaa agaggctac aaagttgtg ggcgagcct 
      ttgagcact tgtgaagat acgggtcag tggagtggc 
      gatgttcct caatgcgtc tttgtggat tgtggtaac 
      ccggaaaat atccaaaac ggtaaagtt accttaacc 
      tcgaacgca acgtactat ggagcactg gccctctac 
      tcctgcgac aaaaacttc gaactggat ggagtttca 
      cgccgattg tgtcttgaa aatggtacc tggagctcc 
      gacgctcca acatgtcgc ggaatccag tgcaaagaa 
      cccgacagg tttgacgga atgacggtc aaagttagt 
      acttacagt gttggggga gttgttgcc tattcatgt 
      ccccgtggg cacaccatg caggggaat gcaactaga 
      gtgtgcttg ctgaagggg gtttggagc ggacgggcg 
      cccacttgc accgcggtc gattgtaaa caccctggt 
      actatcgaa aatgggcga gtgattgtg atgaatggt 
      accacctat aatgcggcg attgagtac cattgtgtc 
      ccaaatttc gaaagagtg gggccgtat ttgcgaaaa 
      tgtatggag aatggggaa tggtcgggt gaggtacca 
      cgatgtgag gagattacc ggggaacca caggaaccc 
      tcgagtctg gggaccaat attggaatt ggggccgga 
      gtggtcgtc tttttgcta attctctta ttagtaatt 
      tatcttaaa ttgcgtaag ccaatccca gtgaagaac 
      accgagaac gtggaaggc gccgagcgc aaagaagac 
      cgaaacgcc gcagtcatg agctacgcc actctgagt 
      gaccgaaac ggtcacttg ccaccaaat atctacgaa 
      aacattcac gatgaaaac atgtacgat gccccctat 
      gaggagaca agtcgcgac agtggcact tacgagccc 
      gaacccatt tcgtggaag aatggaaat atggttacc 
      ataaatggg gtttcagta agatga
    mRNA sequence
    • >TC030754 RA
      aaatggcac aaaacagcg tgttcttcg cctgctgaa 
      ctttcattc cagtattga ccgcggcgg ttaaagcat 
      ggctcttgg ctagaaatg ttcgagtga agtgattag 
      ttaattgtg gtttataaa tgtgttcga acgaattta 
      gcaatgtgg tcaatgaaa tgtcagcca agaaggcct 
      ccatttagt gttttagtg atcttgttt ggattattc 
      aacttcagg aactgtcaa gaaatcaaa tgtgggcat 
      cctgccatt cccttgaac gccagggtc tccctgagc 
      tctcccgga ctcgctccg ggaaccgtg gccaaatac 
      cagtgcgat gagggatat gagacgttt ggtaacacg 
      gaaacttcg tgttcgccg gctggaaaa tggatcggc 
      gagttgccg ttttgtggc accaacatc gcttaccgc 
      aaacccgcc aaccaatca accaccgtt cgcgggggc 
      agcgccctg aacgccaac gacggcgaa aaaagcacc 
      gaacacgac ggaaaacgc tgctccgag acccagaag 
      gaggccagc ccctggtgg cacgtcgac ctcctccag 
      ccctacccc gtgaaggtg gtgcgggtg accaccagg 
      ggctgctgc ggccaccag cccctccag gacctggag 
      atccgggtc ggaaacagc agcactgac ctgcagcgg 
      aaccctctc tgcgcctgg tttccggga acaatagac 
      gaaggcgta acgaagaca ttcacctgt gcacgagtt 
      ttaacagga caatacgtt ttcctgcag ctggtggga 
      gtcgaaggg tcgttgtcg ctctgcgaa gtggaggtt 
      ttcacaacg gacgagttt tccgtggat cgctgcgcc 
      ccgaggggg gcccctgag gacgtccag ttggcgtcc 
      tttgcccgc acttgctac gagttcggg gttagccgc 
      ggagggtcg ttccaggaa gcgcgcaat tactgcagg 
      agccataac ggggacctg gtgcacggc atgagcgca 
      gctgcaacg acgtttctt tatgcggaa ctcgagagg 
      cggaagccg atgctgaag acacaactg gtttggatc 
      ggtgcccaa aaagagcca ggaatcacg tcgagggtc 
      tggaaatgg gttaatggc gacttggtc cagcgtcca 
      gcgtgggga aaagaccaa ccaaacaat tataacggt 
      gaacaaaac tgcgtagtc ctcgacggt ggacgcggg 
      tggttatgg aatgatgta ggttgcaat ctcgactat 
      ctaccatgg atctgtcaa cactctcca tcgtcatgc 
      ggcagtccc gacaaacaa attaacacc accataaaa 
      ggaagcaat tacaacgtc ggctcgagg atcgaatac 
      caatgtcca gaggggcac atgttggtc ggtgaagcg 
      accagactt tgcggaaag gaaggtttc tggtcgggg 
      aaagcgcca acgtgcaaa tatgtgagc tgtgaggct 
      ttgccgggc ttggaacat ggttccgtg gttctaaag 
      gataaacgg acgacatat ggggcacag gctctttac 
      acctgtcac gaaaattac actcttata gggcacgaa 
      atacggact tgtgggtct aatgggact tggacgaat 
      tccacgcct aaatgtctc ttcgactgg tgtccagat 
      ccacctagt attaatggg gggattgtt aaaacgtcg 
      gggcgaagg gctggagac accgcggtt tattcgtgc 
      cagtctggg tttatcttg tttgggcaa ggggtgctt 
      tcgtgcgaa ctggggggc cagtggtcg ggcaaagcc 
      ccctcgtgc aaattcgtg gactgtggg gcccctcca 
      aatatcgac aatggccgg tacgacctg ctcaatggt 
      accacaacc gtcgaaagt ttggtcgaa tacagctgt 
      ggccatgac tactggctc gaagggcaa aaagtgcaa 
      aaatgcaca agggagggc aaatggtca gcggacgca 
      ccctcatgt gaactgata acgtgcgag gaacccgac 
      gttcctgca ggaagttac gtggttggg tacgatttt 
      aacgtacat tcctccatt gagtaccac tgcgaggtg 
      ggacatgtg ttgcgaggg gaacccatc catacgtgt 
      accaaggaa ggggactgg tctggtacc actccaact 
      tgtgaattc gttgattgt ggtaaagta ccgaccatg 
      ctgtacggt actgttaat tatattaat gatactacg 
      taccttgac agtcaaatt gagtattcg tgtgctaag 
      aattacagg cttaatggg gtttcaagg aggcgctgt 
      ttggaaaac aagcagtgg agcaatact ccaccaaag 
      tgtgaagaa ataagatgt cctgagcca attttggcc 
      gaacatagc atactttca gtgactggg aacgatcgc 
      atgtacggt cgtactctt atcaggaca gctgaatcc 
      tcctcaact acttacaaa atcggggcc atagtcaaa 
      tacaggtgt gaaagaggc tacaaagtt gtgggcgag 
      cctttgagc acttgtgaa gatacgggt cagtggagt 
      ggcgatgtt cctcaatgc gtctttgtg gattgtggt 
      aacccggaa aatatccaa aacggtaaa gttacctta 
      acctcgaac gcaacgtac tatggagca ctggccctc 
      tactcctgc gacaaaaac ttcgaactg gatggagtt 
      tcacgccga ttgtgtctt gaaaatggt acctggagc 
      tccgacgct ccaacatgt cgcggaatc cagtgcaaa 
      gaacccgac aggtttgac ggaatgacg gtcaaagtt 
      agtacttac agtgttggg ggagttgtt gcctattca 
      tgtccccgt gggcacacc atgcagggg aatgcaact 
      agagtgtgc ttgctgaag ggggtttgg agcggacgg 
      gcgcccact tgcaccgcg gtcgattgt aaacaccct 
      ggtactatc gaaaatggg cgagtgatt gtgatgaat 
      ggtaccacc tataatgcg gcgattgag taccattgt 
      gtcccaaat ttcgaaaga gtggggccg tatttgcga 
      aaatgtatg gagaatggg gaatggtcg ggtgaggta 
      ccacgatgt gaggagatt accggggaa ccacaggaa 
      ccctcgagt ctggggacc aatattgga attggggcc 
      ggagtggtc gtctttttg ctaattctc ttattagta 
      atttatctt aaattgcgt aagccaatc ccagtgaag 
      aacaccgag aacgtggaa ggcgccgag cgcaaagaa 
      gaccgaaac gccgcagtc atgagctac gccactctg 
      agtgaccga aacggtcac ttgccacca aatatctac 
      gaaaacatt cacgatgaa aacatgtac gatgccccc 
      tatgaggag acaagtcgc gacagtggc acttacgag 
      cccgaaccc atttcgtgg aagaatgga aatatggtt 
      accataaat ggggtttca gtaagatga gatgaaaag 
      aactgaacc aattactgc taccaaaat caacctaga 
      tgttgctta tagagattc tattttttg tttttacta 
      attgatttg taatttaag tcgtgtttg aatgcatta 
      ttcagttat gcggaaatg ttgcgatcg agagatgga 
      aatgtgatt taaattaaa aatcaattg ccttgttgt 
      aaattatgt gtatgataa gctttgtcg taatgactg 
      tgtgagagt gttgtgggg tccacttgt tgatgtaaa 
      tactcattt tgtaataaa ataggttta tgtattgta 
      gataatatc accaataaa agaaaatga cctca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY