Status
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Gene information
General information
TC002855 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 8070941..8092598
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002855
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02700

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002855 RA
      MFYSNINFC SFVSVKENT NGRPQPLLQ EPHYGSRPQ 
      PYNPYPSEQ QRYDEMCAG PGYGEPFGT ETYRKSAPP 
      VTAEYRDQR NTHFQQPGA VLMVYGLDP LRTNADKLF 
      NLMCLYGNV ARIKFLKSK EGTAMVQMG DSVAVERCV 
      QNLNNVTVG LTGAAPLSH HNNVKDDKN NQSEHKLQL 
      AFSKQPFLS EVTNPYPLP DKSPSYKEY ITNKNNRFM 
      NPAMASKNR IQPPSKILH FFNTPPQVT ESELVQVFR 
      DYDVVPPKA VKLFPMKSE RSSSGLLEF EDTTEAVAA 
      VMACNHAAI ESPGTKFPF IMKLCFSSS RSMTIRNGD 
      NNSNGAGEK RIESEH
    CDS sequence
    • >TC002855 RA
      atgttttat tcaaacatt aatttttgt tcttttgtt 
      tcagtgaag gaaaacaca aatggtaga ccgcagcct 
      ttactgcag gagccacat tatggatct cgacctcaa 
      ccttacaat ccctacccc agcgagcag cagaggtac 
      gacgagatg tgcgctggt ccgggctac ggggagcct 
      ttcgggacg gaaacgtac cggaaaagc gctcctccg 
      gttaccgcc gagtaccgg gatcaaagg aacacccac 
      ttccagcag cccggagcc gtcctgatg gtgtacggt 
      ctggaccca cttcgcacc aacgcggac aaactcttt 
      aatctcatg tgcttgtat ggaaatgtc gctcggata 
      aaatttctc aaatcaaaa gaaggcact gccatggtg 
      cagatgggc gacagcgtg gcagtggaa cgatgtgtt 
      caaaattta aacaacgta accgtaggt ctaactggt 
      gccgcgccc ttatcacac cacaacaac gtcaaagac 
      gacaagaac aaccaatcc gaacacaaa ctacaactc 
      gccttcagt aaacaaccc tttcttagt gaagtaaca 
      aatccctat cccttacct gataagtca ccgtcgtat 
      aaagagtac ataacaaat aagaacaac aggtttatg 
      aacccggcg atggccagc aaaaaccgc atccagccc 
      ccgagcaag atcctgcat tttttcaac acaccgcca 
      caagtaacg gaatcagaa ctagtccaa gtatttcgg 
      gattacgac gtcgttcct cccaaagcg gttaaactt 
      tttcctatg aagtcggaa cgatcgagt tcgggactc 
      ctcgaattc gaagacacc acagaggct gttgctgcc 
      gtgatggcg tgcaatcac gccgccatc gagagtcca 
      ggcacaaag ttccctttc ataatgaaa ctgtgtttc 
      tcgtcgtcc cgtagtatg acaattcga aatggtgac 
      aacaattcc aatggagct ggagagaaa cgtatagag 
      tctgaacat taa
    mRNA sequence
    • >TC002855 RA
      attgtattt tacggccag caagttaag atgtacact 
      gtctgggaa tgttttatt caaacatta atttttgtt 
      cttttgttt cagtgaagg aaaacacaa atggtagac 
      cgcagcctt tactgcagg agccacatt atggatctc 
      gacctcaac cttacaatc cctacccca gcgagcagc 
      agaggtacg acgagatgt gcgctggtc cgggctacg 
      gggagcctt tcgggacgg aaacgtacc ggaaaagcg 
      ctcctccgg ttaccgccg agtaccggg atcaaagga 
      acacccact tccagcagc ccggagccg tcctgatgg 
      tgtacggtc tggacccac ttcgcacca acgcggaca 
      aactcttta atctcatgt gcttgtatg gaaatgtcg 
      ctcggataa aatttctca aatcaaaag aaggcactg 
      ccatggtgc agatgggcg acagcgtgg cagtggaac 
      gatgtgttc aaaatttaa acaacgtaa ccgtaggtc 
      taactggtg ccgcgccct tatcacacc acaacaacg 
      tcaaagacg acaagaaca accaatccg aacacaaac 
      tacaactcg ccttcagta aacaaccct ttcttagtg 
      aagtaacaa atccctatc ccttacctg ataagtcac 
      cgtcgtata aagagtaca taacaaata agaacaaca 
      ggtttatga acccggcga tggccagca aaaaccgca 
      tccagcccc cgagcaaga tcctgcatt ttttcaaca 
      caccgccac aagtaacgg aatcagaac tagtccaag 
      tatttcggg attacgacg tcgttcctc ccaaagcgg 
      ttaaacttt ttcctatga agtcggaac gatcgagtt 
      cgggactcc tcgaattcg aagacacca cagaggctg 
      ttgctgccg tgatggcgt gcaatcacg ccgccatcg 
      agagtccag gcacaaagt tccctttca taatgaaac 
      tgtgtttct cgtcgtccc gtagtatga caattcgaa 
      atggtgaca acaattcca atggagctg gagagaaac 
      gtatagagt ctgaacatt aaaagtagt agctccata 
      tccctttaa tataaccga tacatatcc tttccatat 
      accgtatgt ccataatac gttattacc gttagttga 
      attaaggta cttaaggta tctgtttga tcagtagat 
      tgaatctcg ttaaatgaa catgttgtt atgttctaa 
      atatttttt aattgttga tattattta tttctttta 
      ttttgttga aaccaacat attcaagcc gtattttgc 
      aattcccta aaatggact agttaaatt agtattaca 
      tatcttaaa gaaattgac atgaatgtt acaaagatt 
      tgtgtgcaa cacgagttt tagggcaat ttttaaatg 
      tttaattgc aacgacatt agacgtaat gttttttaa 
      ataaagtag gttatttat tgttagaac gatgaacta 
      aggaatagt actgtatat ttttatttg cactaatta 
      tcacccata gtactataa aaggaggtt tgttaagtt 
      gttgttatt tattttcgt ccattgctt gtatcatat 
      cttcattaa agaaataaa gtttcttct tttcattca 
      tttgatatg tgattagtg tatactata ttttatctc 
      gatcttgtg tatatagtt ttgagaatt tttagttgt 
      tacgttcgt attgtaaat atttaaata actgaagtc 
      aatatctaa taactctaa attcagaaa tatctaagc 
      agaaactac gaaactgag caaataaga aatttacca 
      tatttagga acatatcaa ttgtactat taagaatat 
      tttgaaagt ctatttttt ctgtgcttt gtacttttc 
      atatatact ttgatagaa aggaggcta cgatccata 
      gatctaacg aactgctta taaaatgac agcttcaga 
      ttaaaggct tagctaatg agagtttcg tcctgtgaa 
      tctaatgtc actttagtt aaatgtctt cctaacata 
      tgtgtctca gtctaacta ggacctaaa tttgacaaa 
      attgcgtta gctgggacc gatcatgtg ttattgatg 
      tttataata gtgtttgtg tatgggata ttaagacat 
      tgtgcaata aaagtttgc atagttatt atctttcga 
      ccaccatat tgttaacgt gtttttttc cttttttta 
      atatggtgt agttgatga tttcaaaac ataaaagac 
      aaatagtta gttcatatc acatagtat taattattc 
      gtactatag atttagagt tatttttag tgtgcagtg 
      gtctctagc tgtcaaaca gcggtagct aatcagatt 
      tattccgta cttatggac caaaaaaag ttggaatga 
      gaaattgtt aaagccggc ttaaggcct ttgcacact 
      gcgtacagt tactgttgt aggagactc aaaactatc 
      gatagatat tggtgattt ctaggtgat aaagtgatt 
      ttcattgat tatattttt ttaagaaag tggttccgt 
      tgaatgaac acatatcca aaaactctc tcaagaaat 
      ctatcacta agtgttaat ttttctttc ggcatggca 
      tatcaaata atcaatgaa aaatcaagc tcttcatgt 
      gtgttatta attttgtag tattctata aaactgcat 
      tctataata tatatgtcg taacaaaac attagttga 
      gtcaattat ctgtgccta tagcagtgg tagtctatc 
      ttcgtctat cacactctt actccaact ctaaacctt 
      tactacctt gtttctctc tgtgctgtg ataaatttg 
      acatgaatt aactccaag actgcgatt tattataag 
      ctctacaaa gcaattgta aataagctt cta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY