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Gene information
General information
TC003582 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid16: 3485854..3489489
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003582
    iBeetle screen iB_02699
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003582 RA
      MDVETEGTD AKEPSSPAL SNCSRSRSR SVEKNVSNG 
      VSSPAHSTG SGSRKSASR SRSRSRSPQ MNGDTSRNS 
      KSRSRSGSP AGRQSRSRS RSGSARSGS RSPSGSPRR 
      SRSRSASQG SRKAASRSK SRSRSRSGT PAQRNSRSR 
      SPSGSRKGS RSRSRSGSA KSVGSRKSR SRSGSAKSV 
      HSRKSRSRS GSAKSAGSR RSRSRSGSA KSVGSAKSG 
      GSRRSRSRS GSAKSGGSR RSRSRSGSA KSGGSRRSR 
      SRSGSAKSG GSRRSRSRS GSAKSAGSR KSRSRSGSA 
      RSNKSGSRS RSRSRSRSG SRSGSARSR SGSRRSRSG 
      SARSRSGSR SRSRSGSRR SRSGSRSRS RSASGSRKS 
      RSRSRSRSG SAKSRSRSR SGSYSGNEG GVGRKRRLV 
      SDSEDEGEG PPKAKRAVV SDDEGGEPS EEKKIGGEP 
      EEAGNEVVP DVSEDSDDE DRPTHESND GLSDFEVML 
      QRKREEQSK KRKRKDIDI INDNDDIIA QLLADMRNA 
      AEDDRRLNQ LSQPATKKI AMLSKVMSQ LKKHDLQLA 
      FIEHNVLSV LTDWLAPMP DRSLPSLQV REAILKLLA 
      DFPKVDQST RKHSGIGKA VMYLYKHPK ETKENKERA 
      GKLISEWVR PIFNLSADF KAMTREERL QRDLEQIPK 
      RGRNEQQSS SERKELTKA LNSEGKPLR PGEKGWVAR 
      ARVPMPSNK DYLVRPKWT SEVDISRSS KKQMNRFEK 
      HYKNFLDGK RLKQPRRAV EISIEGRNM AL
    CDS sequence
    • >TC003582 RA
      atggatgtt gagactgaa ggaacagat gctaaagaa 
      ccatcttcg ccagctttg agcaactgc tcgcgttcc 
      cgttcgaga tccgtggaa aaaaatgta tcaaatggc 
      gtctcgtct ccagctcat tccacagga tctggatcc 
      cgtaaatcc gcatccagg tcgcgatcc agatcacgt 
      tccccgcaa atgaacgga gacacttct cgtaactcc 
      aaatccagg tccagaagc ggctctcca gctggacgc 
      caatccaga tcaagatcc aggtctggc agcgcacgc 
      tctggctcc agatccccc tcaggatca ccgaggcga 
      tcacgatca cgatccgcg agtcaagga tcacgcaaa 
      gcagcttca cgatcaaaa tcccgctcc agatccaga 
      tcaggaacc cccgcacaa cgtaattcc agatctaga 
      tcgccctca ggctccaga aaagggtcg cgttcgagg 
      tcacgctcg ggttcggcc aaatcggtt ggctctcga 
      aaatcacgc tcacgttca ggttccgca aaatcggtg 
      cattctcga aaatcgcgc tcacgttca ggttctgcg 
      aagtcagct ggttctaga aggtcgcgc tctaggtca 
      ggttctgcc aagtccgtg ggttccgcc aaatccggt 
      ggttccaga aggtcgcgc tctagatct ggttccgcc 
      aaatccggt ggttccaga aggtcgcgc tctagatct 
      ggctccgcg aaatctggc ggttccaga aggtcgcgc 
      tctagatct ggttccgcg aaatctggc ggttccaga 
      aggtcgcgt tcaaggtcg ggatccgcc aaatccgcg 
      ggctccaga aagtcgcgt tcaagatca ggttctgcg 
      cgatctaac aagtcagga tcacgatca cgttcaaga 
      tccaggtcc aggtcgggt tcgagatcc gggtccgcg 
      agatcacgg tctggttct agaagatcg aggtccgga 
      tcggcacgc tctagatcc ggttccaga tcacggtct 
      aggtctgga tccaggcgt tcgaggtct ggctccaga 
      tcgcggtcg cgttctgcg tctggatcg cgcaaatcg 
      cgttcgagg tcaaggtcc agatccgga tccgcgaaa 
      tcacggtca aggtcgcgt tccggttcg tattccgga 
      aacgaggga ggagttggt cggaaacga cgtttggtt 
      tccgactcg gaggacgaa ggtgaaggg ccaccgaag 
      gccaaaagg gcggtagtt agtgatgat gagggtggg 
      gaaccttca gaagagaaa aagatcggg ggggaacca 
      gaagaagct ggaaatgaa gtggttccg gatgtgtct 
      gaagattcc gatgatgag gacagaccc acacacgaa 
      tcaaatgac ggcttatcc gactttgag gtaatgtta 
      caacggaag cgagaagag cagtctaag aaacggaaa 
      cggaaagac atagacatc attaacgac aacgacgac 
      ataatcgca caattatta gccgacatg agaaatgca 
      gctgaagac gaccgaaga ctgaaccaa ctaagccaa 
      cccgccact aaaaaaatc gccatgtta agcaaggtc 
      atgtcgcag ctaaagaaa cacgacttg caattggct 
      ttcatcgaa cataacgtc ctcagcgtg ttgactgac 
      tggttggcc ccaatgccc gaccgttcg ctaccctcg 
      ctgcaagta cgagaggca atactaaaa ctcctagca 
      gacttcccg aaagtcgat cagtccact cgcaaacac 
      tccggaatc ggcaaagcc gtcatgtat ctctataag 
      cacccgaag gagacgaaa gagaacaag gagcgagcc 
      ggcaagtta atcagcgag tgggtgcga cccattttc 
      aatctttcg gccgacttt aaagcaatg actcgtgag 
      gagcgcctt cagagagac ctggagcag attccgaag 
      cgtgggcgg aacgagcaa cagtcgtcc tccgagcgg 
      aaggagttg acgaaagcg ttgaattcg gagggaaag 
      cctctgagg ccgggagaa aaggggtgg gttgcacgg 
      gccagagtt cctatgcct tccaataaa gattatctc 
      gtgcggcct aagtggaca tcggaggtg gacatcagt 
      aggtcgtcg aagaaacaa atgaatagg tttgagaag 
      cattacaag aacttcctg gacggcaaa cgattgaaa 
      caaccaaga agagcagta gaaatatcg attgaaggg 
      cgaaatatg gcgttgtaa
    mRNA sequence
    • >TC003582 RA
      ggtcataaa agtcagtca actgtcaaa aagaacggt 
      gctaaaaat cataaatta gtgatttat tgcgaaacg 
      tattgtttt ttgtgaaaa gtgagcaca aaaacagtt 
      acactctta tcacaattg tgcaataaa gcccccaga 
      agtgtaaaa atatggatg ttgagactg aaggaacag 
      atgctaaag aaccatctt cgccagctt tgagcaact 
      gctcgcgtt cccgttcga gatccgtgg aaaaaaatg 
      tatcaaatg gcgtctcgt ctccagctc attccacag 
      gatctggat cccgtaaat ccgcatcca ggtcgcgat 
      ccagatcac gttccccgc aaatgaacg gagacactt 
      ctcgtaact ccaaatcca ggtccagaa gcggctctc 
      cagctggac gccaatcca gatcaagat ccaggtctg 
      gcagcgcac gctctggct ccagatccc cctcaggat 
      caccgaggc gatcacgat cacgatccg cgagtcaag 
      gatcacgca aagcagctt cacgatcaa aatcccgct 
      ccagatcca gatcaggaa cccccgcac aacgtaatt 
      ccagatcta gatcgccct caggctcca gaaaagggt 
      cgcgttcga ggtcacgct cgggttcgg ccaaatcgg 
      ttggctctc gaaaatcac gctcacgtt caggttccg 
      caaaatcgg tgcattctc gaaaatcgc gctcacgtt 
      caggttctg cgaagtcag ctggttcta gaaggtcgc 
      gctctaggt caggttctg ccaagtccg tgggttccg 
      ccaaatccg gtggttcca gaaggtcgc gctctagat 
      ctggttccg ccaaatccg gtggttcca gaaggtcgc 
      gctctagat ctggctccg cgaaatctg gcggttcca 
      gaaggtcgc gctctagat ctggttccg cgaaatctg 
      gcggttcca gaaggtcgc gttcaaggt cgggatccg 
      ccaaatccg cgggctcca gaaagtcgc gttcaagat 
      caggttctg cgcgatcta acaagtcag gatcacgat 
      cacgttcaa gatccaggt ccaggtcgg gttcgagat 
      ccgggtccg cgagatcac ggtctggtt ctagaagat 
      cgaggtccg gatcggcac gctctagat ccggttcca 
      gatcacggt ctaggtctg gatccaggc gttcgaggt 
      ctggctcca gatcgcggt cgcgttctg cgtctggat 
      cgcgcaaat cgcgttcga ggtcaaggt ccagatccg 
      gatccgcga aatcacggt caaggtcgc gttccggtt 
      cgtattccg gaaacgagg gaggagttg gtcggaaac 
      gacgtttgg tttccgact cggaggacg aaggtgaag 
      ggccaccga aggccaaaa gggcggtag ttagtgatg 
      atgagggtg gggaacctt cagaagaga aaaagatcg 
      ggggggaac cagaagaag ctggaaatg aagtggttc 
      cggatgtgt ctgaagatt ccgatgatg aggacagac 
      ccacacacg aatcaaatg acggcttat ccgactttg 
      aggtaatgt tacaacgga agcgagaag agcagtcta 
      agaaacgga aacggaaag acatagaca tcattaacg 
      acaacgacg acataatcg cacaattat tagccgaca 
      tgagaaatg cagctgaag acgaccgaa gactgaacc 
      aactaagcc aacccgcca ctaaaaaaa tcgccatgt 
      taagcaagg tcatgtcgc agctaaaga aacacgact 
      tgcaattgg ctttcatcg aacataacg tcctcagcg 
      tgttgactg actggttgg ccccaatgc ccgaccgtt 
      cgctaccct cgctgcaag tacgagagg caatactaa 
      aactcctag cagacttcc cgaaagtcg atcagtcca 
      ctcgcaaac actccggaa tcggcaaag ccgtcatgt 
      atctctata agcacccga aggagacga aagagaaca 
      aggagcgag ccggcaagt taatcagcg agtgggtgc 
      gacccattt tcaatcttt cggccgact ttaaagcaa 
      tgactcgtg aggagcgcc ttcagagag acctggagc 
      agattccga agcgtgggc ggaacgagc aacagtcgt 
      cctccgagc ggaaggagt tgacgaaag cgttgaatt 
      cggagggaa agcctctga ggccgggag aaaaggggt 
      gggttgcac gggccagag ttcctatgc cttccaata 
      aagattatc tcgtgcggc ctaagtgga catcggagg 
      tggacatca gtaggtcgt cgaagaaac aaatgaata 
      ggtttgaga agcattaca agaacttcc tggacggca 
      aacgattga aacaaccaa gaagagcag tagaaatat 
      cgattgaag ggcgaaata tggcgttgt aaaggttta 
      tagccaagc gattactta attgtgttt gaattaagt 
      acagtttta agttatttg atacccgtc ttatgtgat 
      gaagtttta gttctatta tgttaggta aatgattct 
      acttatctc tttcttttt acacatacg atttaaacg 
      aagtttgct tgtgttccc gattgagat tctttataa 
      tgttattga accgctttt atctgtatg aagtattaa 
      ataaatttt tatagagaa a