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Gene information
General information
TC003582 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 8047141..8050776
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC003582
    iBeetle screen iB_02699

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003582 RA
      MDVETEGTD AKEPSSPAL SNCSRSRSR SVEKNVSNG 
VSSPAHSTG SGSRKSASR SRSRSRSPQ MNGDTSRNS 
KSRSRSGSP AGRQSRSRS RSGSARSGS RSPSGSPRR 
SRSRSASQG SRKAASRSK SRSRSRSGT PAQRNSRSR 
SPSGSRKGS RSRSRSGSA KSVGSRKSR SRSGSAKSV 
HSRKSRSRS GSAKSAGSR RSRSRSGSA KSVGSAKSG 
GSRRSRSRS GSAKSGGSR RSRSRSGSA KSGGSRRSR 
SRSGSAKSG GSRRSRSRS GSAKSAGSR KSRSRSGSA 
RSNKSGSRS RSRSRSRSG SRSGSARSR SGSRRSRSG 
SARSRSGSR SRSRSGSRR SRSGSRSRS RSASGSRKS 
RSRSRSRSG SAKSRSRSR SGSYSGNEG GVGRKRRLV 
SDSEDEGEG PPKAKRAVV SDDEGGEPS EEKKIGGEP 
EEAGNEVVP DVSEDSDDE DRPTHESND GLSDFEVML 
QRKREEQSK KRKRKDIDI INDNDDIIA QLLADMRNA 
AEDDRRLNQ LSQPATKKI AMLSKVMSQ LKKHDLQLA 
FIEHNVLSV LTDWLAPMP DRSLPSLQV REAILKLLA 
DFPKVDQST RKHSGIGKA VMYLYKHPK ETKENKERA 
GKLISEWVR PIFNLSADF KAMTREERL QRDLEQIPK 
RGRNEQQSS SERKELTKA LNSEGKPLR PGEKGWVAR 
ARVPMPSNK DYLVRPKWT SEVDISRSS KKQMNRFEK 
HYKNFLDGK RLKQPRRAV EISIEGRNM AL
    CDS sequence
    • >TC003582 RA
      atggatgtt gagactgaa ggaacagat gctaaagaa 
ccatcttcg ccagctttg agcaactgc tcgcgttcc 
cgttcgaga tccgtggaa aaaaatgta tcaaatggc 
gtctcgtct ccagctcat tccacagga tctggatcc 
cgtaaatcc gcatccagg tcgcgatcc agatcacgt 
tccccgcaa atgaacgga gacacttct cgtaactcc 
aaatccagg tccagaagc ggctctcca gctggacgc 
caatccaga tcaagatcc aggtctggc agcgcacgc 
tctggctcc agatccccc tcaggatca ccgaggcga 
tcacgatca cgatccgcg agtcaagga tcacgcaaa 
gcagcttca cgatcaaaa tcccgctcc agatccaga 
tcaggaacc cccgcacaa cgtaattcc agatctaga 
tcgccctca ggctccaga aaagggtcg cgttcgagg 
tcacgctcg ggttcggcc aaatcggtt ggctctcga 
aaatcacgc tcacgttca ggttccgca aaatcggtg 
cattctcga aaatcgcgc tcacgttca ggttctgcg 
aagtcagct ggttctaga aggtcgcgc tctaggtca 
ggttctgcc aagtccgtg ggttccgcc aaatccggt 
ggttccaga aggtcgcgc tctagatct ggttccgcc 
aaatccggt ggttccaga aggtcgcgc tctagatct 
ggctccgcg aaatctggc ggttccaga aggtcgcgc 
tctagatct ggttccgcg aaatctggc ggttccaga 
aggtcgcgt tcaaggtcg ggatccgcc aaatccgcg 
ggctccaga aagtcgcgt tcaagatca ggttctgcg 
cgatctaac aagtcagga tcacgatca cgttcaaga 
tccaggtcc aggtcgggt tcgagatcc gggtccgcg 
agatcacgg tctggttct agaagatcg aggtccgga 
tcggcacgc tctagatcc ggttccaga tcacggtct 
aggtctgga tccaggcgt tcgaggtct ggctccaga 
tcgcggtcg cgttctgcg tctggatcg cgcaaatcg 
cgttcgagg tcaaggtcc agatccgga tccgcgaaa 
tcacggtca aggtcgcgt tccggttcg tattccgga 
aacgaggga ggagttggt cggaaacga cgtttggtt 
tccgactcg gaggacgaa ggtgaaggg ccaccgaag 
gccaaaagg gcggtagtt agtgatgat gagggtggg 
gaaccttca gaagagaaa aagatcggg ggggaacca 
gaagaagct ggaaatgaa gtggttccg gatgtgtct 
gaagattcc gatgatgag gacagaccc acacacgaa 
tcaaatgac ggcttatcc gactttgag gtaatgtta 
caacggaag cgagaagag cagtctaag aaacggaaa 
cggaaagac atagacatc attaacgac aacgacgac 
ataatcgca caattatta gccgacatg agaaatgca 
gctgaagac gaccgaaga ctgaaccaa ctaagccaa 
cccgccact aaaaaaatc gccatgtta agcaaggtc 
atgtcgcag ctaaagaaa cacgacttg caattggct 
ttcatcgaa cataacgtc ctcagcgtg ttgactgac 
tggttggcc ccaatgccc gaccgttcg ctaccctcg 
ctgcaagta cgagaggca atactaaaa ctcctagca 
gacttcccg aaagtcgat cagtccact cgcaaacac 
tccggaatc ggcaaagcc gtcatgtat ctctataag 
cacccgaag gagacgaaa gagaacaag gagcgagcc 
ggcaagtta atcagcgag tgggtgcga cccattttc 
aatctttcg gccgacttt aaagcaatg actcgtgag 
gagcgcctt cagagagac ctggagcag attccgaag 
cgtgggcgg aacgagcaa cagtcgtcc tccgagcgg 
aaggagttg acgaaagcg ttgaattcg gagggaaag 
cctctgagg ccgggagaa aaggggtgg gttgcacgg 
gccagagtt cctatgcct tccaataaa gattatctc 
gtgcggcct aagtggaca tcggaggtg gacatcagt 
aggtcgtcg aagaaacaa atgaatagg tttgagaag 
cattacaag aacttcctg gacggcaaa cgattgaaa 
caaccaaga agagcagta gaaatatcg attgaaggg 
cgaaatatg gcgttgtaa
    mRNA sequence
    • >TC003582 RA
      ggtcataaa agtcagtca actgtcaaa aagaacggt 
gctaaaaat cataaatta gtgatttat tgcgaaacg 
tattgtttt ttgtgaaaa gtgagcaca aaaacagtt 
acactctta tcacaattg tgcaataaa gcccccaga 
agtgtaaaa atatggatg ttgagactg aaggaacag 
atgctaaag aaccatctt cgccagctt tgagcaact 
gctcgcgtt cccgttcga gatccgtgg aaaaaaatg 
tatcaaatg gcgtctcgt ctccagctc attccacag 
gatctggat cccgtaaat ccgcatcca ggtcgcgat 
ccagatcac gttccccgc aaatgaacg gagacactt 
ctcgtaact ccaaatcca ggtccagaa gcggctctc 
cagctggac gccaatcca gatcaagat ccaggtctg 
gcagcgcac gctctggct ccagatccc cctcaggat 
caccgaggc gatcacgat cacgatccg cgagtcaag 
gatcacgca aagcagctt cacgatcaa aatcccgct 
ccagatcca gatcaggaa cccccgcac aacgtaatt 
ccagatcta gatcgccct caggctcca gaaaagggt 
cgcgttcga ggtcacgct cgggttcgg ccaaatcgg 
ttggctctc gaaaatcac gctcacgtt caggttccg 
caaaatcgg tgcattctc gaaaatcgc gctcacgtt 
caggttctg cgaagtcag ctggttcta gaaggtcgc 
gctctaggt caggttctg ccaagtccg tgggttccg 
ccaaatccg gtggttcca gaaggtcgc gctctagat 
ctggttccg ccaaatccg gtggttcca gaaggtcgc 
gctctagat ctggctccg cgaaatctg gcggttcca 
gaaggtcgc gctctagat ctggttccg cgaaatctg 
gcggttcca gaaggtcgc gttcaaggt cgggatccg 
ccaaatccg cgggctcca gaaagtcgc gttcaagat 
caggttctg cgcgatcta acaagtcag gatcacgat 
cacgttcaa gatccaggt ccaggtcgg gttcgagat 
ccgggtccg cgagatcac ggtctggtt ctagaagat 
cgaggtccg gatcggcac gctctagat ccggttcca 
gatcacggt ctaggtctg gatccaggc gttcgaggt 
ctggctcca gatcgcggt cgcgttctg cgtctggat 
cgcgcaaat cgcgttcga ggtcaaggt ccagatccg 
gatccgcga aatcacggt caaggtcgc gttccggtt 
cgtattccg gaaacgagg gaggagttg gtcggaaac 
gacgtttgg tttccgact cggaggacg aaggtgaag 
ggccaccga aggccaaaa gggcggtag ttagtgatg 
atgagggtg gggaacctt cagaagaga aaaagatcg 
ggggggaac cagaagaag ctggaaatg aagtggttc 
cggatgtgt ctgaagatt ccgatgatg aggacagac 
ccacacacg aatcaaatg acggcttat ccgactttg 
aggtaatgt tacaacgga agcgagaag agcagtcta 
agaaacgga aacggaaag acatagaca tcattaacg 
acaacgacg acataatcg cacaattat tagccgaca 
tgagaaatg cagctgaag acgaccgaa gactgaacc 
aactaagcc aacccgcca ctaaaaaaa tcgccatgt 
taagcaagg tcatgtcgc agctaaaga aacacgact 
tgcaattgg ctttcatcg aacataacg tcctcagcg 
tgttgactg actggttgg ccccaatgc ccgaccgtt 
cgctaccct cgctgcaag tacgagagg caatactaa 
aactcctag cagacttcc cgaaagtcg atcagtcca 
ctcgcaaac actccggaa tcggcaaag ccgtcatgt 
atctctata agcacccga aggagacga aagagaaca 
aggagcgag ccggcaagt taatcagcg agtgggtgc 
gacccattt tcaatcttt cggccgact ttaaagcaa 
tgactcgtg aggagcgcc ttcagagag acctggagc 
agattccga agcgtgggc ggaacgagc aacagtcgt 
cctccgagc ggaaggagt tgacgaaag cgttgaatt 
cggagggaa agcctctga ggccgggag aaaaggggt 
gggttgcac gggccagag ttcctatgc cttccaata 
aagattatc tcgtgcggc ctaagtgga catcggagg 
tggacatca gtaggtcgt cgaagaaac aaatgaata 
ggtttgaga agcattaca agaacttcc tggacggca 
aacgattga aacaaccaa gaagagcag tagaaatat 
cgattgaag ggcgaaata tggcgttgt aaaggttta 
tagccaagc gattactta attgtgttt gaattaagt 
acagtttta agttatttg atacccgtc ttatgtgat 
gaagtttta gttctatta tgttaggta aatgattct 
acttatctc tttcttttt acacatacg atttaaacg 
aagtttgct tgtgttccc gattgagat tctttataa 
tgttattga accgctttt atctgtatg aagtattaa 
ataaatttt tatagagaa a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY