| General information | |||
| TC003582 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 8047141..8050776 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC003582 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02699 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC003582 RA
MDVETEGTD AKEPSSPAL SNCSRSRSR SVEKNVSNG VSSPAHSTG SGSRKSASR SRSRSRSPQ MNGDTSRNS KSRSRSGSP AGRQSRSRS RSGSARSGS RSPSGSPRR SRSRSASQG SRKAASRSK SRSRSRSGT PAQRNSRSR SPSGSRKGS RSRSRSGSA KSVGSRKSR SRSGSAKSV HSRKSRSRS GSAKSAGSR RSRSRSGSA KSVGSAKSG GSRRSRSRS GSAKSGGSR RSRSRSGSA KSGGSRRSR SRSGSAKSG GSRRSRSRS GSAKSAGSR KSRSRSGSA RSNKSGSRS RSRSRSRSG SRSGSARSR SGSRRSRSG SARSRSGSR SRSRSGSRR SRSGSRSRS RSASGSRKS RSRSRSRSG SAKSRSRSR SGSYSGNEG GVGRKRRLV SDSEDEGEG PPKAKRAVV SDDEGGEPS EEKKIGGEP EEAGNEVVP DVSEDSDDE DRPTHESND GLSDFEVML QRKREEQSK KRKRKDIDI INDNDDIIA QLLADMRNA AEDDRRLNQ LSQPATKKI AMLSKVMSQ LKKHDLQLA FIEHNVLSV LTDWLAPMP DRSLPSLQV REAILKLLA DFPKVDQST RKHSGIGKA VMYLYKHPK ETKENKERA GKLISEWVR PIFNLSADF KAMTREERL QRDLEQIPK RGRNEQQSS SERKELTKA LNSEGKPLR PGEKGWVAR ARVPMPSNK DYLVRPKWT SEVDISRSS KKQMNRFEK HYKNFLDGK RLKQPRRAV EISIEGRNM AL
- >TC003582 RA
atggatgtt gagactgaa ggaacagat gctaaagaa ccatcttcg ccagctttg agcaactgc tcgcgttcc cgttcgaga tccgtggaa aaaaatgta tcaaatggc gtctcgtct ccagctcat tccacagga tctggatcc cgtaaatcc gcatccagg tcgcgatcc agatcacgt tccccgcaa atgaacgga gacacttct cgtaactcc aaatccagg tccagaagc ggctctcca gctggacgc caatccaga tcaagatcc aggtctggc agcgcacgc tctggctcc agatccccc tcaggatca ccgaggcga tcacgatca cgatccgcg agtcaagga tcacgcaaa gcagcttca cgatcaaaa tcccgctcc agatccaga tcaggaacc cccgcacaa cgtaattcc agatctaga tcgccctca ggctccaga aaagggtcg cgttcgagg tcacgctcg ggttcggcc aaatcggtt ggctctcga aaatcacgc tcacgttca ggttccgca aaatcggtg cattctcga aaatcgcgc tcacgttca ggttctgcg aagtcagct ggttctaga aggtcgcgc tctaggtca ggttctgcc aagtccgtg ggttccgcc aaatccggt ggttccaga aggtcgcgc tctagatct ggttccgcc aaatccggt ggttccaga aggtcgcgc tctagatct ggctccgcg aaatctggc ggttccaga aggtcgcgc tctagatct ggttccgcg aaatctggc ggttccaga aggtcgcgt tcaaggtcg ggatccgcc aaatccgcg ggctccaga aagtcgcgt tcaagatca ggttctgcg cgatctaac aagtcagga tcacgatca cgttcaaga tccaggtcc aggtcgggt tcgagatcc gggtccgcg agatcacgg tctggttct agaagatcg aggtccgga tcggcacgc tctagatcc ggttccaga tcacggtct aggtctgga tccaggcgt tcgaggtct ggctccaga tcgcggtcg cgttctgcg tctggatcg cgcaaatcg cgttcgagg tcaaggtcc agatccgga tccgcgaaa tcacggtca aggtcgcgt tccggttcg tattccgga aacgaggga ggagttggt cggaaacga cgtttggtt tccgactcg gaggacgaa ggtgaaggg ccaccgaag gccaaaagg gcggtagtt agtgatgat gagggtggg gaaccttca gaagagaaa aagatcggg ggggaacca gaagaagct ggaaatgaa gtggttccg gatgtgtct gaagattcc gatgatgag gacagaccc acacacgaa tcaaatgac ggcttatcc gactttgag gtaatgtta caacggaag cgagaagag cagtctaag aaacggaaa cggaaagac atagacatc attaacgac aacgacgac ataatcgca caattatta gccgacatg agaaatgca gctgaagac gaccgaaga ctgaaccaa ctaagccaa cccgccact aaaaaaatc gccatgtta agcaaggtc atgtcgcag ctaaagaaa cacgacttg caattggct ttcatcgaa cataacgtc ctcagcgtg ttgactgac tggttggcc ccaatgccc gaccgttcg ctaccctcg ctgcaagta cgagaggca atactaaaa ctcctagca gacttcccg aaagtcgat cagtccact cgcaaacac tccggaatc ggcaaagcc gtcatgtat ctctataag cacccgaag gagacgaaa gagaacaag gagcgagcc ggcaagtta atcagcgag tgggtgcga cccattttc aatctttcg gccgacttt aaagcaatg actcgtgag gagcgcctt cagagagac ctggagcag attccgaag cgtgggcgg aacgagcaa cagtcgtcc tccgagcgg aaggagttg acgaaagcg ttgaattcg gagggaaag cctctgagg ccgggagaa aaggggtgg gttgcacgg gccagagtt cctatgcct tccaataaa gattatctc gtgcggcct aagtggaca tcggaggtg gacatcagt aggtcgtcg aagaaacaa atgaatagg tttgagaag cattacaag aacttcctg gacggcaaa cgattgaaa caaccaaga agagcagta gaaatatcg attgaaggg cgaaatatg gcgttgtaa
- >TC003582 RA
ggtcataaa agtcagtca actgtcaaa aagaacggt gctaaaaat cataaatta gtgatttat tgcgaaacg tattgtttt ttgtgaaaa gtgagcaca aaaacagtt acactctta tcacaattg tgcaataaa gcccccaga agtgtaaaa atatggatg ttgagactg aaggaacag atgctaaag aaccatctt cgccagctt tgagcaact gctcgcgtt cccgttcga gatccgtgg aaaaaaatg tatcaaatg gcgtctcgt ctccagctc attccacag gatctggat cccgtaaat ccgcatcca ggtcgcgat ccagatcac gttccccgc aaatgaacg gagacactt ctcgtaact ccaaatcca ggtccagaa gcggctctc cagctggac gccaatcca gatcaagat ccaggtctg gcagcgcac gctctggct ccagatccc cctcaggat caccgaggc gatcacgat cacgatccg cgagtcaag gatcacgca aagcagctt cacgatcaa aatcccgct ccagatcca gatcaggaa cccccgcac aacgtaatt ccagatcta gatcgccct caggctcca gaaaagggt cgcgttcga ggtcacgct cgggttcgg ccaaatcgg ttggctctc gaaaatcac gctcacgtt caggttccg caaaatcgg tgcattctc gaaaatcgc gctcacgtt caggttctg cgaagtcag ctggttcta gaaggtcgc gctctaggt caggttctg ccaagtccg tgggttccg ccaaatccg gtggttcca gaaggtcgc gctctagat ctggttccg ccaaatccg gtggttcca gaaggtcgc gctctagat ctggctccg cgaaatctg gcggttcca gaaggtcgc gctctagat ctggttccg cgaaatctg gcggttcca gaaggtcgc gttcaaggt cgggatccg ccaaatccg cgggctcca gaaagtcgc gttcaagat caggttctg cgcgatcta acaagtcag gatcacgat cacgttcaa gatccaggt ccaggtcgg gttcgagat ccgggtccg cgagatcac ggtctggtt ctagaagat cgaggtccg gatcggcac gctctagat ccggttcca gatcacggt ctaggtctg gatccaggc gttcgaggt ctggctcca gatcgcggt cgcgttctg cgtctggat cgcgcaaat cgcgttcga ggtcaaggt ccagatccg gatccgcga aatcacggt caaggtcgc gttccggtt cgtattccg gaaacgagg gaggagttg gtcggaaac gacgtttgg tttccgact cggaggacg aaggtgaag ggccaccga aggccaaaa gggcggtag ttagtgatg atgagggtg gggaacctt cagaagaga aaaagatcg ggggggaac cagaagaag ctggaaatg aagtggttc cggatgtgt ctgaagatt ccgatgatg aggacagac ccacacacg aatcaaatg acggcttat ccgactttg aggtaatgt tacaacgga agcgagaag agcagtcta agaaacgga aacggaaag acatagaca tcattaacg acaacgacg acataatcg cacaattat tagccgaca tgagaaatg cagctgaag acgaccgaa gactgaacc aactaagcc aacccgcca ctaaaaaaa tcgccatgt taagcaagg tcatgtcgc agctaaaga aacacgact tgcaattgg ctttcatcg aacataacg tcctcagcg tgttgactg actggttgg ccccaatgc ccgaccgtt cgctaccct cgctgcaag tacgagagg caatactaa aactcctag cagacttcc cgaaagtcg atcagtcca ctcgcaaac actccggaa tcggcaaag ccgtcatgt atctctata agcacccga aggagacga aagagaaca aggagcgag ccggcaagt taatcagcg agtgggtgc gacccattt tcaatcttt cggccgact ttaaagcaa tgactcgtg aggagcgcc ttcagagag acctggagc agattccga agcgtgggc ggaacgagc aacagtcgt cctccgagc ggaaggagt tgacgaaag cgttgaatt cggagggaa agcctctga ggccgggag aaaaggggt gggttgcac gggccagag ttcctatgc cttccaata aagattatc tcgtgcggc ctaagtgga catcggagg tggacatca gtaggtcgt cgaagaaac aaatgaata ggtttgaga agcattaca agaacttcc tggacggca aacgattga aacaaccaa gaagagcag tagaaatat cgattgaag ggcgaaata tggcgttgt aaaggttta tagccaagc gattactta attgtgttt gaattaagt acagtttta agttatttg atacccgtc ttatgtgat gaagtttta gttctatta tgttaggta aatgattct acttatctc tttcttttt acacatacg atttaaacg aagtttgct tgtgttccc gattgagat tctttataa tgttattga accgctttt atctgtatg aagtattaa ataaatttt tatagagaa a
