Status
Please wait ...
Gene information
General information
TC032383
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC003461
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 5416389..5422792
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032383
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02698

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature lateral partiallynot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature ventral partiallynot present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: Dorsal musculature seems to be less affected.  
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (16 dpi)
    Days post injection



    Pupal morphology
    oblique dorsal thoracic muscles median slightlybroader - Number of animals showing the phenotype: 7

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032383 RA
      MDSKNNLEV SQTLNDNST EFKNKAQEL LAELQTKAS 
      SLEDYVNDF KKSVDEDEA EIEEVQRLQ PEVERHTKN 
      LTEKAMLLD NLLNESRDL SGEAVRAAN AYKDIVQAV 
      KNASEAAKK AKIDVNKAY DMIPDVANK TDNADQTSS 
      VSLKDAFEL YHSTNDDLK PKLEEAVAK YKPVSSSHK 
      SNKDMLDNI EKIRDKIQQ QSLDKAYAL AVDKADDAI 
      RHVTNIEGI AEGIKFDKL KNQTENATL LPKIVDDMN 
      RDLLQTQKQ LNTVNDKLP YITNMMEEI PEQQARLQR 
      TVDNIANSM KKLNQQIIL ARDLANQIK IGMKFYPNT 
      TLELEVPSN LEELSTSER VSGYFRTEK PYGLLWYLG 
      NPLGTKLRR TKTDDYMAL IIQNGYPVL KLDVGNGVE 
      QIINEKYVS DGVWYQFIV ERTGHNAKL TIREELPGG 
      VENITQKET TLEGPLSIF NLDKNKSKL FVGSFPVNY 
      AMQKEIDVN SFEGEIEDM VIGDKPVSL WNFNNGFEN 
      NHGAVERDK LVNLQPSTG FRFNGNGYA ILNARSYGF 
      RTRSDIQLS FKTFATEGL LFLAGQDKT FIALELRNG 
      KVLYQYNLG HQTKVWHTT KTYNDGRWH VVEASREGP 
      KGRFVVDNE DIPDTTPAI DGTFIEPIE TVSFGGYPT 
      KHRYIDVSQ TRFDGCIDN VVIMGSPVD LRNNIKVYD 
      VTPGCPVKF SQMVSFDKH RPGYVAHGG VAINNDFKI 
      SLKFKTKEK DGLIFYATD KLHENNISL ALRDGYLVL 
      ISQKIELVS KDTFNDSLW HVVSVMHND HELRIDFDD 
      IDSRPLDTK PPPLFILHG TLYVGGLPR RVILSPGTV 
      GSELPFRGC IGDLTLKGN VINFANSTD KFNEILGKC 
      IYDKNYKAI DKIDKEPTL PPLEPLGPA TTGGYGDVT 
      KYRPYEHTT IRGDGGRDD YVTRTDEEP VTEVVPLPP 
      LPPVTHGAV GYPPLTTPP PPPKDSCAL PISPPQTSV 
      GVYRFGAQK DSRLEIDKL RGRYKKQFD FALDFRSDD 
      PDGIIFFTS DNNETHSQY VAVFLQDGQ VVYTYSDGR 
      SSSVLKTDG LKDYSDNAW HLVEFSKDE RRAKLVLDD 
      AVVSDKPVA NMQKIDIRP PIYVGGLDP NHYNKIQSK 
      LNLTKSFSG CIRNMNMNS KPLEFQAQN QIGVVPCFD 
      KIETGVFFP VTGGFVRIK EPKFKVGEV FNVKLDIKP 
      RRDSGVLIA VNGRKDYFV LEMINGEMS LTVENGKGP 
      IRAVYNPNK KFYFCDGQW HSIQAVKSK NVITLSVDN 
      VFTDPKLGD HSSTSTDTA SALFLGGHR YLNATRARG 
      IVTRLPYVG CVKNIFINN DPVEVDASM AEGGVIVGT 
      CPTN
    CDS sequence
    • >TC032383 RA
      atggactcg aaaaacaat ttggaggtc tcgcaaact 
      ttgaatgat aattcgacc gaattcaaa aacaaggcc 
      caagaattg ttagcggaa ttacagacg aaggcttcg 
      agtctggaa gattacgtc aatgatttt aaaaagtcg 
      gtcgatgag gacgaggcc gaaatcgag gaagtgcag 
      cgtttgcag cccgaagtt gaaagacac actaaaaac 
      ttgaccgaa aaagcaatg cttcttgat aatctctta 
      aacgagtcg cgagacttg tcaggcgag gccgtccgt 
      gcggcgaac gcctacaaa gacatagtc caagcggtg 
      aaaaatgcg agcgaagcc gcgaaaaaa gcaaaaatt 
      gacgtaaat aaggcctac gacatgata cccgacgtt 
      gcgaataaa accgacaac gcggaccaa acgtcttcg 
      gtgtcgcta aaggacgcc ttcgaacta taccattca 
      acgaacgat gacttgaaa ccgaagcta gaggaggcc 
      gttgccaaa tacaagccg gtcagttcc agtcacaag 
      tcaaataaa gacatgttg gacaacatc gagaaaatt 
      cgcgacaaa atccaacaa cagtccttg gataaggcg 
      tacgccctc gctgttgac aaggccgac gatgcgatc 
      cggcacgtg acgaacatt gagggcatc gcggaggga 
      attaagttc gacaagttg aaaaaccag acggaaaat 
      gcaacttta ttgcccaaa atcgtcgat gatatgaac 
      cgcgatttg ctacaaacg cagaaacag ctgaatact 
      gttaacgac aagctgccc tatatcacc aacatgatg 
      gaggagata ccagaacag caagcccgg cttcaacga 
      acggttgac aacattgcc aattcgatg aagaagttg 
      aaccaacag ataatcctg gcgcgagat ttggccaat 
      cagatcaaa ataggaatg aagttttac ccgaacacg 
      actctggaa cttgaggtt ccgtcgaat ctggaggaa 
      ctcagcact tccgaacgc gtttctggg tatttcagg 
      acggagaaa ccgtatgga ctgctgtgg tacttgggc 
      aatcccctg gggactaaa ttaaggcga actaaaacg 
      gatgactac atggccttg atcatacaa aacggttat 
      cccgtcttg aaactggac gttggcaac ggagtcgag 
      caaataatc aacgagaaa tacgtttct gacggtgtg 
      tggtatcaa ttcatagtg gagagaact ggtcacaat 
      gcgaagttg acgatccgc gaggagttg cccggaggt 
      gttgagaac atcacccag aaggaaacc acactggaa 
      ggcccctta tccattttc aatctggac aagaacaag 
      tcgaaactg ttcgtcgga agcttcccc gtgaactac 
      gcgatgcag aaagaaatc gatgtgaat tcctttgag 
      ggcgaaatt gaggatatg gttattggc gacaaaccg 
      gtttcgttg tggaatttc aacaacggt tttgagaac 
      aatcacgga gccgtggaa cgggataag ttggttaac 
      ttgcaacca agtaccggg tttaggttt aacgggaat 
      ggttatgcc attttgaac gcgcgttcg tacgggttt 
      aggactcga tctgacatc caactcagc ttcaagacg 
      tttgcaacc gaaggtttg ctcttcttg gcggggcaa 
      gacaagacg tttattgcg ctggaactc aggaatggg 
      aaggttctg taccaatat aatttgggg caccaaacc 
      aaagtgtgg cacacgaca aagacctac aacgatggc 
      agatggcac gtggtggag gctagtcga gaggggccc 
      aaaggccgc tttgtggtt gataatgag gatatacca 
      gataccacc cctgcaata gacggcacg tttattgag 
      ccaatcgaa accgtttca tttggtggt tatcccacc 
      aaacaccgg tacatcgat gtcagtcag acgcgattc 
      gatggctgc attgataac gttgtgata atgggcagt 
      ccggttgat ttacgcaac aatattaaa gtgtacgat 
      gtcacaccc ggatgtccg gtcaaattc tcacaaatg 
      gtgtcgttt gataaacac agaccggga tatgtcgcc 
      cacggcggc gtggccatc aataacgat ttcaagata 
      agtctcaaa tttaaaacc aaagaaaaa gacggtttg 
      attttctac gccacggat aagctgcac gaaaataac 
      atttcactg gccctccgg gacggttat ctcgtccta 
      atcagccag aaaatcgaa ctggtttcg aaagacacg 
      ttcaatgat agcctctgg cacgttgtc tcggtgatg 
      cacaacgat cacgagtta agaattgat tttgacgat 
      atcgacagc agacctctg gacaccaag cctccaccg 
      ttatttatc ctccacggt actctatac gtaggcggc 
      ttacccaga cgggtcatt ttatctcct ggtaccgtg 
      ggctcggag ttaccattc agaggttgc atcggtgat 
      ctcactttg aaaggcaac gtcatcaat tttgctaac 
      agtacggac aagtttaac gaaatttta ggaaaatgt 
      atctatgac aaaaattac aaagctatc gataaaatt 
      gataaagag ccgacattg ccacctctt gagccactg 
      ggacctgcc acaactggg ggctatgga gacgtgacg 
      aagtaccgg ccatatgaa catactacc attcggggt 
      gacggcggc agagacgac tacgtgact cgaacagac 
      gaggaacca gtgactgaa gtggttcca ttgccgcct 
      cttccaccg gtcactcat ggggctgtt ggttaccca 
      ccgctcact acacctcca ccacctccc aaagactca 
      tgtgctttg cctatttct cctccgcaa actagtgta 
      ggggtgtac cgattcggt gctcagaaa gacagtaga 
      ctagaaata gataaattg cgcggtcgg tacaagaaa 
      caattcgat tttgctctt gactttagg agtgatgac 
      ccagacggc atcattttc tttacgagc gataacaac 
      gagacgcat tcgcagtac gtggcagtg ttcttgcaa 
      gatggacaa gtggtttac acgtatagt gacggcagg 
      agttcgtcg gtgttgaag acagacggt ttgaaagac 
      tacagcgat aacgcgtgg cacttggtc gagtttagc 
      aaagacgag cgtcgagca aagctagtc ttggatgac 
      gcagtcgtg tctgataag cctgtagcc aacatgcaa 
      aaaattgat atcaggccc ccgatctac gtcggggga 
      ttggacccc aaccattat aacaaaatt caaagcaaa 
      ctgaacctg actaagtcg tttagcggc tgcattcgc 
      aacatgaac atgaacagc aagcctcta gaattccag 
      gcacagaac cagatcgga gtagtcccc tgctttgat 
      aaaatcgaa accggggtg ttcttcccc gtaacggga 
      ggttttgtc aggattaaa gaaccgaag tttaaagtc 
      ggagaagtg ttcaatgtg aaacttgac atcaagccg 
      aggcgagat tcgggagtt ttgattgct gttaatggg 
      cggaaagac tatttcgtc ttggaaatg atcaatggt 
      gagatgagt ctgacggtg gaaaatgga aaaggcccg 
      attagagcc gtttacaac cccaataag aagttctac 
      ttctgcgac ggtcaatgg cacagcatt caagctgtt 
      aaatcgaaa aatgttata accctgtcg gttgataat 
      gtgtttact gatcccaaa ctcggggat cactcgtcg 
      acttccaca gacaccgcc agtgctctc ttcttaggc 
      ggacaccga tatttgaac gcaactagg gcacgggga 
      attgtcaca aggttgcca tatgttgga tgtgttaaa 
      aacattttt attaacaat gacccggtc gaggttgat 
      gcaagtatg gcagaaggg ggtgttatt gtgggcact 
      tgcccgact aattaa
    mRNA sequence
    • >TC032383 RA
      taaatttta acacgaaat tgatcaatg ctctaaatt 
      acagtaaag aacggctta agaaaaaac tggacgata 
      ttaagcaac aaacaatgg actcgaaaa acaatttgg 
      aggtctcgc aaactttga atgataatt cgaccgaat 
      tcaaaaaca aggcccaag aattgttag cggaattac 
      agacgaagg cttcgagtc tggaagatt acgtcaatg 
      attttaaaa agtcggtcg atgaggacg aggccgaaa 
      tcgaggaag tgcagcgtt tgcagcccg aagttgaaa 
      gacacacta aaaacttga ccgaaaaag caatgcttc 
      ttgataatc tcttaaacg agtcgcgag acttgtcag 
      gcgaggccg tccgtgcgg cgaacgcct acaaagaca 
      tagtccaag cggtgaaaa atgcgagcg aagccgcga 
      aaaaagcaa aaattgacg taaataagg cctacgaca 
      tgatacccg acgttgcga ataaaaccg acaacgcgg 
      accaaacgt cttcggtgt cgctaaagg acgccttcg 
      aactatacc attcaacga acgatgact tgaaaccga 
      agctagagg aggccgttg ccaaataca agccggtca 
      gttccagtc acaagtcaa ataaagaca tgttggaca 
      acatcgaga aaattcgcg acaaaatcc aacaacagt 
      ccttggata aggcgtacg ccctcgctg ttgacaagg 
      ccgacgatg cgatccggc acgtgacga acattgagg 
      gcatcgcgg agggaatta agttcgaca agttgaaaa 
      accagacgg aaaatgcaa ctttattgc ccaaaatcg 
      tcgatgata tgaaccgcg atttgctac aaacgcaga 
      aacagctga atactgtta acgacaagc tgccctata 
      tcaccaaca tgatggagg agataccag aacagcaag 
      cccggcttc aacgaacgg ttgacaaca ttgccaatt 
      cgatgaaga agttgaacc aacagataa tcctggcgc 
      gagatttgg ccaatcaga tcaaaatag gaatgaagt 
      tttacccga acacgactc tggaacttg aggttccgt 
      cgaatctgg aggaactca gcacttccg aacgcgttt 
      ctgggtatt tcaggacgg agaaaccgt atggactgc 
      tgtggtact tgggcaatc ccctgggga ctaaattaa 
      ggcgaacta aaacggatg actacatgg ccttgatca 
      tacaaaacg gttatcccg tcttgaaac tggacgttg 
      gcaacggag tcgagcaaa taatcaacg agaaatacg 
      tttctgacg gtgtgtggt atcaattca tagtggaga 
      gaactggtc acaatgcga agttgacga tccgcgagg 
      agttgcccg gaggtgttg agaacatca cccagaagg 
      aaaccacac tggaaggcc ccttatcca ttttcaatc 
      tggacaaga acaagtcga aactgttcg tcggaagct 
      tccccgtga actacgcga tgcagaaag aaatcgatg 
      tgaattcct ttgagggcg aaattgagg atatggtta 
      ttggcgaca aaccggttt cgttgtgga atttcaaca 
      acggttttg agaacaatc acggagccg tggaacggg 
      ataagttgg ttaacttgc aaccaagta ccgggttta 
      ggtttaacg ggaatggtt atgccattt tgaacgcgc 
      gttcgtacg ggtttagga ctcgatctg acatccaac 
      tcagcttca agacgtttg caaccgaag gtttgctct 
      tcttggcgg ggcaagaca agacgttta ttgcgctgg 
      aactcagga atgggaagg ttctgtacc aatataatt 
      tggggcacc aaaccaaag tgtggcaca cgacaaaga 
      cctacaacg atggcagat ggcacgtgg tggaggcta 
      gtcgagagg ggcccaaag gccgctttg tggttgata 
      atgaggata taccagata ccacccctg caatagacg 
      gcacgttta ttgagccaa tcgaaaccg tttcatttg 
      gtggttatc ccaccaaac accggtaca tcgatgtca 
      gtcagacgc gattcgatg gctgcattg ataacgttg 
      tgataatgg gcagtccgg ttgatttac gcaacaata 
      ttaaagtgt acgatgtca cacccggat gtccggtca 
      aattctcac aaatggtgt cgtttgata aacacagac 
      cgggatatg tcgcccacg gcggcgtgg ccatcaata 
      acgatttca agataagtc tcaaattta aaaccaaag 
      aaaaagacg gtttgattt tctacgcca cggataagc 
      tgcacgaaa ataacattt cactggccc tccgggacg 
      gttatctcg tcctaatca gccagaaaa tcgaactgg 
      tttcgaaag acacgttca atgatagcc tctggcacg 
      ttgtctcgg tgatgcaca acgatcacg agttaagaa 
      ttgattttg acgatatcg acagcagac ctctggaca 
      ccaagcctc caccgttat ttatcctcc acggtactc 
      tatacgtag gcggcttac ccagacggg tcattttat 
      ctcctggta ccgtgggct cggagttac cattcagag 
      gttgcatcg gtgatctca ctttgaaag gcaacgtca 
      tcaattttg ctaacagta cggacaagt ttaacgaaa 
      ttttaggaa aatgtatct atgacaaaa attacaaag 
      ctatcgata aaattgata aagagccga cattgccac 
      ctcttgagc cactgggac ctgccacaa ctgggggct 
      atggagacg tgacgaagt accggccat atgaacata 
      ctaccattc ggggtgacg gcggcagag acgactacg 
      tgactcgaa cagacgagg aaccagtga ctgaagtgg 
      ttccattgc cgcctcttc caccggtca ctcatgggg 
      ctgttggtt acccaccgc tcactacac ctccaccac 
      ctcccaaag actcatgtg ctttgccta tttctcctc 
      cgcaaacta gtgtagggg tgtaccgat tcggtgctc 
      agaaagaca gtagactag aaatagata aattgcgcg 
      gtcggtaca agaaacaat tcgattttg ctcttgact 
      ttaggagtg atgacccag acggcatca ttttcttta 
      cgagcgata acaacgaga cgcattcgc agtacgtgg 
      cagtgttct tgcaagatg gacaagtgg tttacacgt 
      atagtgacg gcaggagtt cgtcggtgt tgaagacag 
      acggtttga aagactaca gcgataacg cgtggcact 
      tggtcgagt ttagcaaag acgagcgtc gagcaaagc 
      tagtcttgg atgacgcag tcgtgtctg ataagcctg 
      tagccaaca tgcaaaaaa ttgatatca ggcccccga 
      tctacgtcg ggggattgg accccaacc attataaca 
      aaattcaaa gcaaactga acctgacta agtcgttta 
      gcggctgca ttcgcaaca tgaacatga acagcaagc 
      ctctagaat tccaggcac agaaccaga tcggagtag 
      tcccctgct ttgataaaa tcgaaaccg gggtgttct 
      tccccgtaa cgggaggtt ttgtcagga ttaaagaac 
      cgaagttta aagtcggag aagtgttca atgtgaaac 
      ttgacatca agccgaggc gagattcgg gagttttga 
      ttgctgtta atgggcgga aagactatt tcgtcttgg 
      aaatgatca atggtgaga tgagtctga cggtggaaa 
      atggaaaag gcccgatta gagccgttt acaacccca 
      ataagaagt tctacttct gcgacggtc aatggcaca 
      gcattcaag ctgttaaat cgaaaaatg ttataaccc 
      tgtcggttg ataatgtgt ttactgatc ccaaactcg 
      gggatcact cgtcgactt ccacagaca ccgccagtg 
      ctctcttct taggcggac accgatatt tgaacgcaa 
      ctagggcac ggggaattg tcacaaggt tgccatatg 
      ttggatgtg ttaaaaaca tttttatta acaatgacc 
      cggtcgagg ttgatgcaa gtatggcag aagggggtg 
      ttattgtgg gcacttgcc cgactaatt aagaaataa 
      atcattcat cttcactca cgctttttt tatttagca 
      attaaccaa atattttta agcaattat gtatccgat 
      ttttacttt gccaaataa atgctttac ttagaagta 
      cacaaagga aagtgcatt tcatcgtga tcacaaaga 
      cctgacact gttcaaaca ttaatttca tataaaatc 
      aaccactct tccaatcac ttcgcaaaa atgcgtatc 
      attgctctc tgcttgatt gtgtccctt ccttgggtt 
      caatccaga gacgctatt caatttttc atagctcac 
      aaaaaagtg aataaggcc tctattgaa gttcttgaa 
      aatgtggac aactcgagc caagtttgg ttcgagtat 
      aagtatatt tttgcaacc gaagacgaa catgtatta 
      aacacgact ctaaaaatt ggagtgttt gcagaaaaa 
      gcatcaacg tttgatttt ctcgattgt cggatgacg 
      atcaacgat aatcaaaca atgatcgaa ggttacagt 
      attgacata attgaaaat tttcgagaa gccttaaat 
      atcaggtaa aacatccct aatccttag tgcattgag 
      aggagtatt aaactgttt tctttatcg agaaaaatg 
      acactttag atagagcca atgaatttt taattccag 
      cactgaatt caaagtaat aaacaattt aactcaact 
      tttcaacaa cgaaattga tttagtaat ctgccaccc 
      acattctga gaatttgcc ctcaaatgt aaccgagtt 
      atacccagt cataaagag caagtacgt tatttaaac 
      cagtgaaaa atccttgca tgcgttact caattgtag 
      tttgatatt ggtttacaa acccagcaa gttgcgatt 
      caccaaaga tatattcat tttaacgtt tgccggctc 
      actgtggtt ggttttctt tgcaataat gatccttct 
      ggctgtatg tttacgaat ctcaacccg aaaatacgc 
      aatgatgcg accaagtta cgaaacatg gacgtgggt 
      tgagataac actttgggc cattgccgc tgcgtgcca 
      acaaggtaa aaaaattca aaatttgtc cttacggaa 
      ccaaaattt aaaggccta agtcacaca ccgagcggt 
      tttgccagt tgcttgata tttttcctg ggatatctg 
      atggcgtat ttattgtac accggtttt gcggctgct 
      ataacttca attctggtg gaaaacgtc gaaactaac 
      gctaattta cagaacctc agaatcgat aaacttcac 
      ttgatcagt cctgagtgc acccggagc gagataaaa 
      caattcaaa gaaagattt gtaatttct gagtttatg 
      tgaacgact ttgcaacaa ctgtttcag cttgtaaca 
      gaatacgtc tctgactta aatgctcta tttgaaaaa 
      ttggatata aacgacagg atcggtgtg ggccctgaa 
      atttcatta cagcagtac atggtgaat aatttgggc 
      ctcgacgaa attctatcc ttgcaaaca atgaaactg 
      aatgtgaga tacacccaa ggttttctg acgctgcgt 
      aataattcg tggaaaaaa aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY