Status
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Gene information
General information
TC003424 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4821318..4882480
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC003424
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_02696

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC003424 RA
        MSPIENVVP NLVDNSTNR DVELRKQDR SRDLFGVQL 
        EVTSLLLEA ERRKLSVLQ KELQVALEE GGSIKAKEH 
        QKQELLVAI TRIQAQHDH LDKEYREHA LGALLSANT 
        RGLGKVSEV IKMQECSKP EKPKIIGPK PSIPSKPKI 
        VPPVNVRGQ RVVKGNERC LVSDSRRAS LSEHKPKEI 
        PKEVSRSLS QTEKREVFS TRTSQSLHP KSASVETNV 
        KYDEYSATY ISEKCDTHT SFEAYKKIE EVTQKAPNS 
        PSTVCCSIL SNVTTDCCG IINVNKKEL NGKTMTKID 
        SLDSNSSDS GGFKDFIQL DLVKAEPQV PCHQRKASQ 
        PEFLADKAF GHQRKASQP DHLAPFAHN KQTFVANAQ 
        ALAQFLPQT EQKLLQHRP DDSFRQQIA KFSAVQKTD 
        DTKARPIPQ IISQSQFQQ STRKLEELL SQRLEKDKV 
        IRKGQNCLL DGESSSQDV EQKMMVQKQ MQQKLQADL 
        QQTVKQIQE IQSIELRLP QNRKWSESN RSQPAIGLK 
        HTPKSRVSH PIISFPNSQ RSQNDSKRK SLSDSISAG 
        RVCTIAMAA TVQNDSFRQ FHGVCRNVR T
      • >TC003424 RB
        MSPIENVVP NLVDNSTNR DVELRKQDR SRDLFGVQL 
        EVTSLLLEA ERRKLSVLQ KELQVALEE GGSIKAKEH 
        QKQELLVAI TRIQAQHDH LDKEYREHA LGALLSANT 
        RGLGKVSEV IKMQECSKP EKPKIIGPK PSIPSKPKI 
        VPPVNVRGQ RVVKGNERC LVSDSRRAS LSEHKPKEI 
        PKEVSRSLS QTEKREVFS TRTSQSLHP KSASVETNV 
        KYDEYSATY ISEKCDTHT SFEAYKKIE EVTQKAPNS 
        PSTVCCSIL SNVTTDCCG IINVNKKEL NGKTMTKID 
        SLDSNSSDS GGFKDFIQL DLVKAEPQV PCHQRKASQ 
        PEFLADKAF GHQRKASQP DHLAPFAHN KQTFVANAQ 
        ALAQFLPQT EQKLLQHRP DDSFRQQIA KFSAVQKTD 
        DTKARPIPQ IISQSQFQQ STRKLEELL SQRLEKDKV 
        IRKGQNCLL DGESSSQDV EQKMMVQKQ MQQKLQADL 
        QQTVKQIQE IQSIELRLP QNRKWSEVC VCLVLNGVT 
        HIQGV
      CDS sequence
      • >TC003424 RA
        atgagtccc attgagaac gtggttccg aacttggtg 
        gataattcc accaaccga gacgtggag ctcaggaaa 
        caggaccga tcccgtgac cttttcggt gttcaattg 
        gaggtgacg agcctcctt ctcgaggcg gaaaggagg 
        aaattatcg gttcttcag aaagaacta caagtcgct 
        ttggaagaa ggagggtca attaaagcc aaggaacac 
        cagaaacaa gaacttcta gttgccata acgcgtatt 
        caggcgcag cacgaccac ttagacaaa gaatatcgt 
        gaacatgcg ctaggagct ttactttct gcaaataca 
        agaggatta ggaaaggta agcgaagtg attaaaatg 
        caagagtgc agcaagccc gagaaacca aaaataatc 
        ggacctaag ccgtcaatt ccgtcgaag cccaaaatt 
        gtgccacct gtgaatgtc cggggccaa agggttgtt 
        aagggcaac gagcggtgt ttggtgagc gactcgaga 
        agagcatcg ctgtccgaa cacaagccc aaagaaatc 
        cccaaggag gtgagccga tctttgtcg caaaccgaa 
        aaaagggag gttttttcc acacggact tcacaaagt 
        ctgcatccg aaaagtgcc agcgttgaa acgaacgtc 
        aagtatgac gagtattca gccacttac attagcgaa 
        aaatgtgat acacacact agcttcgaa gcctataag 
        aaaatcgaa gaggtgacc cagaaagcc cccaactcc 
        cccagcacc gtctgctgc agtatctta tccaacgta 
        acgaccgac tgctgcggt ataatcaac gtaaacaaa 
        aaagagctc aacggcaaa accatgact aaaatcgac 
        tccctcgac tccaactcg tccgactcc ggcggcttc 
        aaggacttc atccagctg gacctcgtc aaggccgag 
        ccccaggtc ccctgccac cagcgcaag gcctcgcag 
        ccggagttc ctcgccgac aaggccttc ggccaccag 
        cggaaggcc tcccagccc gaccacctc gcccccttc 
        gcccacaac aaacaaacc ttcgtggcc aacgcacaa 
        gccttagcg cagtttttg ccgcagact gaacagaaa 
        ctcctccag cacaggccc gacgactcc ttccgccag 
        cagatcgcc aaattcagc gcagtgcaa aagactgac 
        gacaccaaa gcgaggccc ataccacaa atcatatcg 
        cagagccag ttccagcag tccaccagg aagctggag 
        gagctgctg tcccagcgg ctggagaag gacaaggtc 
        atcaggaaa gggcagaat tgtctgctt gacggcgag 
        tctagcagc caggacgtg gagcagaaa atgatggtg 
        cagaagcag atgcagcag aagttgcaa gcggatttg 
        caacaaact gttaaacaa atccaggag atacaaagc 
        atcgaactg cgattgccg caaaaccga aaatggagc 
        gagagtaac aggagccag cccgccatc ggcctgaaa 
        cacacacca aaatcaagg gtaagtcac ccaataatt 
        tccttccca aattcgcaa cggtcacaa aatgattca 
        aaaagaaaa tccctctcc gactccatc agcgcaggc 
        cgcgtctgt acaatcgca atggcggcg actgtacaa 
        aatgactca ttccgtcag tttcacggt gtctgtcgg 
        aacgtacgc acgtag
      • >TC003424 RB
        atgagtccc attgagaac gtggttccg aacttggtg 
        gataattcc accaaccga gacgtggag ctcaggaaa 
        caggaccga tcccgtgac cttttcggt gttcaattg 
        gaggtgacg agcctcctt ctcgaggcg gaaaggagg 
        aaattatcg gttcttcag aaagaacta caagtcgct 
        ttggaagaa ggagggtca attaaagcc aaggaacac 
        cagaaacaa gaacttcta gttgccata acgcgtatt 
        caggcgcag cacgaccac ttagacaaa gaatatcgt 
        gaacatgcg ctaggagct ttactttct gcaaataca 
        agaggatta ggaaaggta agcgaagtg attaaaatg 
        caagagtgc agcaagccc gagaaacca aaaataatc 
        ggacctaag ccgtcaatt ccgtcgaag cccaaaatt 
        gtgccacct gtgaatgtc cggggccaa agggttgtt 
        aagggcaac gagcggtgt ttggtgagc gactcgaga 
        agagcatcg ctgtccgaa cacaagccc aaagaaatc 
        cccaaggag gtgagccga tctttgtcg caaaccgaa 
        aaaagggag gttttttcc acacggact tcacaaagt 
        ctgcatccg aaaagtgcc agcgttgaa acgaacgtc 
        aagtatgac gagtattca gccacttac attagcgaa 
        aaatgtgat acacacact agcttcgaa gcctataag 
        aaaatcgaa gaggtgacc cagaaagcc cccaactcc 
        cccagcacc gtctgctgc agtatctta tccaacgta 
        acgaccgac tgctgcggt ataatcaac gtaaacaaa 
        aaagagctc aacggcaaa accatgact aaaatcgac 
        tccctcgac tccaactcg tccgactcc ggcggcttc 
        aaggacttc atccagctg gacctcgtc aaggccgag 
        ccccaggtc ccctgccac cagcgcaag gcctcgcag 
        ccggagttc ctcgccgac aaggccttc ggccaccag 
        cggaaggcc tcccagccc gaccacctc gcccccttc 
        gcccacaac aaacaaacc ttcgtggcc aacgcacaa 
        gccttagcg cagtttttg ccgcagact gaacagaaa 
        ctcctccag cacaggccc gacgactcc ttccgccag 
        cagatcgcc aaattcagc gcagtgcaa aagactgac 
        gacaccaaa gcgaggccc ataccacaa atcatatcg 
        cagagccag ttccagcag tccaccagg aagctggag 
        gagctgctg tcccagcgg ctggagaag gacaaggtc 
        atcaggaaa gggcagaat tgtctgctt gacggcgag 
        tctagcagc caggacgtg gagcagaaa atgatggtg 
        cagaagcag atgcagcag aagttgcaa gcggatttg 
        caacaaact gttaaacaa atccaggag atacaaagc 
        atcgaactg cgattgccg caaaaccga aaatggagc 
        gaggtttgt gtttgtttg gttttgaac ggcgtcaca 
        catatacag ggtgtctga
      mRNA sequence
      • >TC003424 RA
        cgttcgctg tgcaacagg tgattgtca gtcgccggt 
        aaacatcgt gagtgtagg tttgctgtc ttctctcga 
        ttaacaaac acatgctga ggctttctt cagctgatc 
        ttcgaaaaa tcggaaacg gcaaatatt gcgataaag 
        actaaagta caagtgaca agtgcaagt gttgggtgt 
        ttttgttgt gatttggtc tagagtgct tggtgtatg 
        atgaaactg aaaggatta acttgggct taaagatga 
        gtcccattg agaacgtgg ttccgaact tggtggata 
        attccacca accgagacg tggagctca ggaaacagg 
        accgatccc gtgaccttt tcggtgttc aattggagg 
        tgacgagcc tccttctcg aggcggaaa ggaggaaat 
        tatcggttc ttcagaaag aactacaag tcgctttgg 
        aagaaggag ggtcaatta aagccaagg aacaccaga 
        aacaagaac ttctagttg ccataacgc gtattcagg 
        cgcagcacg accacttag acaaagaat atcgtgaac 
        atgcgctag gagctttac tttctgcaa atacaagag 
        gattaggaa aggtaagcg aagtgatta aaatgcaag 
        agtgcagca agcccgaga aaccaaaaa taatcggac 
        ctaagccgt caattccgt cgaagccca aaattgtgc 
        cacctgtga atgtccggg gccaaaggg ttgttaagg 
        gcaacgagc ggtgtttgg tgagcgact cgagaagag 
        catcgctgt ccgaacaca agcccaaag aaatcccca 
        aggaggtga gccgatctt tgtcgcaaa ccgaaaaaa 
        gggaggttt tttccacac ggacttcac aaagtctgc 
        atccgaaaa gtgccagcg ttgaaacga acgtcaagt 
        atgacgagt attcagcca cttacatta gcgaaaaat 
        gtgatacac acactagct tcgaagcct ataagaaaa 
        tcgaagagg tgacccaga aagccccca actccccca 
        gcaccgtct gctgcagta tcttatcca acgtaacga 
        ccgactgct gcggtataa tcaacgtaa acaaaaaag 
        agctcaacg gcaaaacca tgactaaaa tcgactccc 
        tcgactcca actcgtccg actccggcg gcttcaagg 
        acttcatcc agctggacc tcgtcaagg ccgagcccc 
        aggtcccct gccaccagc gcaaggcct cgcagccgg 
        agttcctcg ccgacaagg ccttcggcc accagcgga 
        aggcctccc agcccgacc acctcgccc ccttcgccc 
        acaacaaac aaaccttcg tggccaacg cacaagcct 
        tagcgcagt ttttgccgc agactgaac agaaactcc 
        tccagcaca ggcccgacg actccttcc gccagcaga 
        tcgccaaat tcagcgcag tgcaaaaga ctgacgaca 
        ccaaagcga ggcccatac cacaaatca tatcgcaga 
        gccagttcc agcagtcca ccaggaagc tggaggagc 
        tgctgtccc agcggctgg agaaggaca aggtcatca 
        ggaaagggc agaattgtc tgcttgacg gcgagtcta 
        gcagccagg acgtggagc agaaaatga tggtgcaga 
        agcagatgc agcagaagt tgcaagcgg atttgcaac 
        aaactgtta aacaaatcc aggagatac aaagcatcg 
        aactgcgat tgccgcaaa accgaaaat ggagcgaga 
        gtaacagga gccagcccg ccatcggcc tgaaacaca 
        caccaaaat caagggtaa gtcacccaa taatttcct 
        tcccaaatt cgcaacggt cacaaaatg attcaaaaa 
        gaaaatccc tctccgact ccatcagcg caggccgcg 
        tctgtacaa tcgcaatgg cggcgactg tacaaaatg 
        actcattcc gtcagtttc acggtgtct gtcggaacg 
        tacgcacgt agtgttccg ttcttctat gtttgtaaa 
        caacatatg accaccaaa gcgcaattt ttgcgtttt 
        ttggcgaaa aaatcgacg cgattttgt attttcccg 
        ctcagtagt cacgaaaca attgtgttg tgttcgttt 
        gtttgacct gtgttgact atgattggg acgttcggt 
        ttgtggtaa ttgtgcttc aagagcggc gcaaatcct 
        aaggtattt ttcattttt tttgcaaga agcaagtca 
        cgattgggt ttttttttt ggtaatttt taatcgaac 
        acggaacag gcaaggaga aagtcttgt tcggtccag 
        tattgtgtg tttagtggc ggaattctc gtctcgtac 
        gagcaggtt tataagcaa ataaaatgt tgagacgca 
        c
      • >TC003424 RB
        gaagaattc acagattgt atttgaatc gtgattctt 
        ttaatgcca tatttcgtg ggaagcgca tcattacaa 
        tgatggtcg caattattt tattagaaa aagtggtcg 
        aggccgcgt atgaaatta tgacattaa ttatttttt 
        ctttgaaaa attctgaca taaatttat aatgttcat 
        tacgctgaa tccacagca agagcaaaa gttgtgtca 
        tgatgtgga gcttctgat gtaaagtcc cgatatgtc 
        aagagctat gatttatgc tttatcttg ttcgttatc 
        actgcaagg cgatttata aaatttaaa tcaggaact 
        gcatgttat gcaaaaagg aacgaaatt atctctgaa 
        tttgcattg agaaatgtg taattgtgc ttgtatcta 
        ttatattta cgacgttat tacgcaact ccagaagat 
        ctttcacct ttgttgtct gtaatgaat ttttaaggg 
        ttttattga accgatgca taatcaaaa agatattat 
        gactgtaat aaaagtaac atcaagatg taattttca 
        tgacctctg taatcatga gtcccattg agaacgtgg 
        ttccgaact tggtggata attccacca accgagacg 
        tggagctca ggaaacagg accgatccc gtgaccttt 
        tcggtgttc aattggagg tgacgagcc tccttctcg 
        aggcggaaa ggaggaaat tatcggttc ttcagaaag 
        aactacaag tcgctttgg aagaaggag ggtcaatta 
        aagccaagg aacaccaga aacaagaac ttctagttg 
        ccataacgc gtattcagg cgcagcacg accacttag 
        acaaagaat atcgtgaac atgcgctag gagctttac 
        tttctgcaa atacaagag gattaggaa aggtaagcg 
        aagtgatta aaatgcaag agtgcagca agcccgaga 
        aaccaaaaa taatcggac ctaagccgt caattccgt 
        cgaagccca aaattgtgc cacctgtga atgtccggg 
        gccaaaggg ttgttaagg gcaacgagc ggtgtttgg 
        tgagcgact cgagaagag catcgctgt ccgaacaca 
        agcccaaag aaatcccca aggaggtga gccgatctt 
        tgtcgcaaa ccgaaaaaa gggaggttt tttccacac 
        ggacttcac aaagtctgc atccgaaaa gtgccagcg 
        ttgaaacga acgtcaagt atgacgagt attcagcca 
        cttacatta gcgaaaaat gtgatacac acactagct 
        tcgaagcct ataagaaaa tcgaagagg tgacccaga 
        aagccccca actccccca gcaccgtct gctgcagta 
        tcttatcca acgtaacga ccgactgct gcggtataa 
        tcaacgtaa acaaaaaag agctcaacg gcaaaacca 
        tgactaaaa tcgactccc tcgactcca actcgtccg 
        actccggcg gcttcaagg acttcatcc agctggacc 
        tcgtcaagg ccgagcccc aggtcccct gccaccagc 
        gcaaggcct cgcagccgg agttcctcg ccgacaagg 
        ccttcggcc accagcgga aggcctccc agcccgacc 
        acctcgccc ccttcgccc acaacaaac aaaccttcg 
        tggccaacg cacaagcct tagcgcagt ttttgccgc 
        agactgaac agaaactcc tccagcaca ggcccgacg 
        actccttcc gccagcaga tcgccaaat tcagcgcag 
        tgcaaaaga ctgacgaca ccaaagcga ggcccatac 
        cacaaatca tatcgcaga gccagttcc agcagtcca 
        ccaggaagc tggaggagc tgctgtccc agcggctgg 
        agaaggaca aggtcatca ggaaagggc agaattgtc 
        tgcttgacg gcgagtcta gcagccagg acgtggagc 
        agaaaatga tggtgcaga agcagatgc agcagaagt 
        tgcaagcgg atttgcaac aaactgtta aacaaatcc 
        aggagatac aaagcatcg aactgcgat tgccgcaaa 
        accgaaaat ggagcgagg tttgtgttt gtttggttt 
        tgaacggcg tcacacata tacagggtg tctgaccta 
        aaactgtcg ggcctgata tctcagata tttgtcaac 
        ggattttta tgaaattta aaatgcaga tattttaga 
        agataaaga ttaaaaccc cattaatgt aagaactca 
        agtcgtcaa aatgtaatg taatgtaat catttaaaa 
        aattcatcg tcaataaaa agtgctgta agaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY