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Gene information
General information
TC003405 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 4347291..4365573
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003405
    iBeetle screen iB_02695

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 10 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003405 RA
      MKSNVTNGT SNNTRYTVV FDQENLNNT EKKKTGWFG 
      TRHFVTFML FLGMANAYV MRTNMSMAI VAMVNHTAI 
      EASHSNHDE PVDTECGVD LPQANKSHQ ENDGEFVWE 
      TDIQGYVLS SFFYGYVVT QIPFGILSK RYGNIYFLG 
      VGMLINSLF GLLVPVAAN MGIWWLIAV RFIQGLGEG 
      PIVPCTHAL LAKWIPPNE RSRMGAFVY AGAQFGTVI 
      SMPLSGLLS ASSAGWPSI FYVFGAVGT VWCVAFLIF 
      VYEDPSTNP KMNIDEKKY IQKALGNVS GAPIPPIPW 
      KSIIKSLPF WAILLAHMG HNYGYETLM TELPTYMKQ 
      VLHFSIKDN GFLSALPYL AMWLFSIFI SHVADWLLT 
      KPCFTHTIV RKLINGIGQ YGPAIALVA ASFTGCDRW 
      LTVAILTIG VGLNGGIYS GFKVNHLDI SPQFAGILM 
      AFTNCLANL AGLLAPIYA GNVVLGTPS QAKWRVVFV 
      TAAGVYAAC CTFYILFGS GERQSWDQQ EDPDKKKEA 
      DSDPEIVTT RA
    • >TC003405 RB
      MKSNVTNGT SNNTRYTVV FDQENLNNT EKKKTGWFG 
      TRHFVTFML FLGMANAYV MRTNMSMAI VAMVNHTAI 
      EASHSNHDE PVDTECGVD LPQANKSHQ ENDGEFVWE 
      TDIQGYVLS SFFYGYVVT QIPFGILSK RYGNIYFLG 
      VGMLINSLF GLLVPVAAN MGIWWLIAV RFIQGLGEG 
      PIVPCTHAL LAKWIPPNE RSRMGAFVY AGIFFCNTE 
      KETVLRMIG FTAR
    • >TC003405 RC
      MPLSGLLSA SSAGWPSIF YVFGAVGTV WCVAFLIFV 
      YEDPSTNPK MNIDEKKYI QKALGNVSG APIPPIPWK 
      SIIKSLPFW AILLAHMGH NYGYETLMT ELPTYMKQV 
      LHFSIKDNG FLSALPYLA MWLFSIFIS HVADWLLTK 
      PCFTHTIVR KLINGIGQY GPAIALVAA SFTGCDRWL 
      TVAILTIGV GLNGGIYSG FKVNHLDIS PQFAGILMA 
      FTNCLANLA GLLAPIYAG NVVLGTPSQ AKWRVVFVT 
      AAGVYAACC TFYILFGSG ERQSWDQQE DPDKKKEAD 
      SDPEIVTTR A
    CDS sequence
    • >TC003405 RA
      atgaaaagc aacgtgacg aatgggacc agcaacaac 
      accagatat acggtcgtt ttcgaccaa gagaacctc 
      aacaacacc gaaaagaaa aaaacagga tggttcggc 
      acgcgccac tttgtgacc ttcatgctg ttcctcgga 
      atggcaaac gcctatgta atgaggacc aacatgtct 
      atggccatc gtggcaatg gtgaaccac acggcaatc 
      gaagcctca cattcgaac cacgacgag ccggttgac 
      accgaatgc ggcgtggat ctgccccag gcgaacaaa 
      tcgcaccag gaaaacgac ggcgaattt gtctgggag 
      acggacatc cagggctac gtcctcagc tccttcttc 
      tacggctac gtcgtcacc cagatcccc ttcggcatc 
      ctctccaag cgctacggc aacatctac ttcctgggc 
      gttggcatg ctgatcaac tccctgttc ggcctcctg 
      gtgcccgtc gccgccaac atgggcatc tggtggctg 
      atagccgtg aggttcatt cagggcctc ggtgagggc 
      cctattgtc ccatgcaca cacgctctt ctggcaaaa 
      tggatccct cctaacgag agaagtcga atgggtgct 
      ttcgtttat gcaggagcg caatttgga acagtcatt 
      tcaatgccg ctgagtggc cttttgtcg gcttcgtcc 
      gccggctgg ccgtccatt ttttacgtt tttggggcc 
      gttgggacc gtctggtgc gtggccttc ctgatcttc 
      gtctacgaa gacccgagc acgaacccc aaaatgaac 
      atcgacgag aaaaaatac atacaaaaa gccttgggg 
      aacgtcagc ggggcacct attccgcca atcccgtgg 
      aagtcgatc atcaaatcc ctgcccttc tgggccatt 
      ctgctggcc catatgggc cataactac gggtatgag 
      accttgatg actgagtta ccgacgtat atgaaacaa 
      gttctacac ttcagtatt aaagacaat gggtttctt 
      tcggctcta ccttaccta gccatgtgg ttgttctcg 
      atcttcatt agtcacgtt gctgattgg ttgttaacc 
      aaaccgtgc ttcacacac actattgtg cgaaagttg 
      atcaacgga attggccaa tatggccct gcgattgcg 
      ttggtggcg gcgtcattc acgggctgt gaccgatgg 
      ttgaccgtg gccatcctc accatcgga gtgggcctg 
      aacggcggt atctactct ggcttcaaa gtcaaccac 
      ttggacatc tccccgcaa ttcgccggg atcctgatg 
      gccttcacc aactgtttg gcaaatctt gccggattg 
      ttagcccca atctacgct ggaaacgta gttttggga 
      acgcctagt caagcgaaa tggcgcgtc gtcttcgtc 
      acggcagcg ggtgtttat gctgcgtgt tgcacgttc 
      tatattctg ttcggctca ggggagcgc cagtcctgg 
      gatcagcag gaggatccc gacaagaag aaggaggca 
      gacagtgac ccggagatt gtgacaaca cgtgcttga
    • >TC003405 RB
      atgaaaagc aacgtgacg aatgggacc agcaacaac 
      accagatat acggtcgtt ttcgaccaa gagaacctc 
      aacaacacc gaaaagaaa aaaacagga tggttcggc 
      acgcgccac tttgtgacc ttcatgctg ttcctcgga 
      atggcaaac gcctatgta atgaggacc aacatgtct 
      atggccatc gtggcaatg gtgaaccac acggcaatc 
      gaagcctca cattcgaac cacgacgag ccggttgac 
      accgaatgc ggcgtggat ctgccccag gcgaacaaa 
      tcgcaccag gaaaacgac ggcgaattt gtctgggag 
      acggacatc cagggctac gtcctcagc tccttcttc 
      tacggctac gtcgtcacc cagatcccc ttcggcatc 
      ctctccaag cgctacggc aacatctac ttcctgggc 
      gttggcatg ctgatcaac tccctgttc ggcctcctg 
      gtgcccgtc gccgccaac atgggcatc tggtggctg 
      atagccgtg aggttcatt cagggcctc ggtgagggc 
      cctattgtc ccatgcaca cacgctctt ctggcaaaa 
      tggatccct cctaacgag agaagtcga atgggtgct 
      ttcgtttat gcaggtata tttttctgc aatactgaa 
      aaagagaca gtgttacga atgataggt tttacggca 
      cgttga
    • >TC003405 RC
      atgccgctg agtggcctt ttgtcggct tcgtccgcc 
      ggctggccg tccattttt tacgttttt ggggccgtt 
      gggaccgtc tggtgcgtg gccttcctg atcttcgtc 
      tacgaagac ccgagcacg aaccccaaa atgaacatc 
      gacgagaaa aaatacata caaaaagcc ttggggaac 
      gtcagcggg gcacctatt ccgccaatc ccgtggaag 
      tcgatcatc aaatccctg cccttctgg gccattctg 
      ctggcccat atgggccat aactacggg tatgagacc 
      ttgatgact gagttaccg acgtatatg aaacaagtt 
      ctacacttc agtattaaa gacaatggg tttctttcg 
      gctctacct tacctagcc atgtggttg ttctcgatc 
      ttcattagt cacgttgct gattggttg ttaaccaaa 
      ccgtgcttc acacacact attgtgcga aagttgatc 
      aacggaatt ggccaatat ggccctgcg attgcgttg 
      gtggcggcg tcattcacg ggctgtgac cgatggttg 
      accgtggcc atcctcacc atcggagtg ggcctgaac 
      ggcggtatc tactctggc ttcaaagtc aaccacttg 
      gacatctcc ccgcaattc gccgggatc ctgatggcc 
      ttcaccaac tgtttggca aatcttgcc ggattgtta 
      gccccaatc tacgctgga aacgtagtt ttgggaacg 
      cctagtcaa gcgaaatgg cgcgtcgtc ttcgtcacg 
      gcagcgggt gtttatgct gcgtgttgc acgttctat 
      attctgttc ggctcaggg gagcgccag tcctgggat 
      cagcaggag gatcccgac aagaagaag gaggcagac 
      agtgacccg gagattgtg acaacacgt gcttga
    mRNA sequence
    • >TC003405 RA
      gtgccccac ttttccccg cgataattt taattgtaa 
      tattgacct ttaaatgtt tgtcgataa ggaagtttc 
      aaagattga gagcaacaa taagccata gaacaaaat 
      ttgaataga tgtgtcggt ggcgacgcg gatttctaa 
      taaaaatat gaagacatc agcgcagaa agcgtcagt 
      gacccaaca cctgttgcg cggtgcaaa ccgcgcgtc 
      cgcgactat attattatt attacaaca aataacgcc 
      gtttaattc cagcagtat tttttaacg caaatctcg 
      tatttttat ttttattta tttattccg gtcttacat 
      tcccacttt tcgcgcaaa atgggccat cacgtgaac 
      ggcttagaa atggaaaag aagaatccc aacgaagaa 
      gagccaccg caaaagatt accaagtag tcttatcgg 
      ttcggttcg ttgtcttcc tgttcagtg aaactgata 
      aaagtgaca acgagcgtt cggtcccaa catttccct 
      cgaaaaaca accggcgga aagttaaaa actgtgctg 
      tgctcgtgt acgtgtcgg agttccgct atcggcaaa 
      cacaaacct ataatgaac gtttgatta tgatttgta 
      ttttcaaat cattccccg atcgtttaa cgatacttt 
      cgtcccgaa aacggaagc ggctcaagt aactcggcc 
      aaatcgcat ccgccattt aaacccgga ttatttttg 
      gcgcctatt tataccgag cccaattat ttatttttt 
      gatttttta cgccgtttt aactcattg agcccagcg 
      aacatgcac ccccatcgc ctctagaac tccgccgaa 
      ttagtttta cccaatttt cgccaaaat gaaaagcaa 
      cgtgacgaa tgggaccag caacaacac cagatatac 
      ggtcgtttt cgaccaaga gaacctcaa caacaccga 
      aaagaaaaa aacaggatg gttcggcac gcgccactt 
      tgtgacctt catgctgtt cctcggaat ggcaaacgc 
      ctatgtaat gaggaccaa catgtctat ggccatcgt 
      ggcaatggt gaaccacac ggcaatcga agcctcaca 
      ttcgaacca cgacgagcc ggttgacac cgaatgcgg 
      cgtggatct gccccaggc gaacaaatc gcaccagga 
      aaacgacgg cgaatttgt ctgggagac ggacatcca 
      gggctacgt cctcagctc cttcttcta cggctacgt 
      cgtcaccca gatcccctt cggcatcct ctccaagcg 
      ctacggcaa catctactt cctgggcgt tggcatgct 
      gatcaactc cctgttcgg cctcctggt gcccgtcgc 
      cgccaacat gggcatctg gtggctgat agccgtgag 
      gttcattca gggcctcgg tgagggccc tattgtccc 
      atgcacaca cgctcttct ggcaaaatg gatccctcc 
      taacgagag aagtcgaat gggtgcttt cgtttatgc 
      aggagcgca atttggaac agtcatttc aatgccgct 
      gagtggcct tttgtcggc ttcgtccgc cggctggcc 
      gtccatttt ttacgtttt tggggccgt tgggaccgt 
      ctggtgcgt ggccttcct gatcttcgt ctacgaaga 
      cccgagcac gaaccccaa aatgaacat cgacgagaa 
      aaaatacat acaaaaagc cttggggaa cgtcagcgg 
      ggcacctat tccgccaat cccgtggaa gtcgatcat 
      caaatccct gcccttctg ggccattct gctggccca 
      tatgggcca taactacgg gtatgagac cttgatgac 
      tgagttacc gacgtatat gaaacaagt tctacactt 
      cagtattaa agacaatgg gtttctttc ggctctacc 
      ttacctagc catgtggtt gttctcgat cttcattag 
      tcacgttgc tgattggtt gttaaccaa accgtgctt 
      cacacacac tattgtgcg aaagttgat caacggaat 
      tggccaata tggccctgc gattgcgtt ggtggcggc 
      gtcattcac gggctgtga ccgatggtt gaccgtggc 
      catcctcac catcggagt gggcctgaa cggcggtat 
      ctactctgg cttcaaagt caaccactt ggacatctc 
      cccgcaatt cgccgggat cctgatggc cttcaccaa 
      ctgtttggc aaatcttgc cggattgtt agccccaat 
      ctacgctgg aaacgtagt tttgggaac gcctagtca 
      agcgaaatg gcgcgtcgt cttcgtcac ggcagcggg 
      tgtttatgc tgcgtgttg cacgttcta tattctgtt 
      cggctcagg ggagcgcca gtcctggga tcagcagga 
      ggatcccga caagaagaa ggaggcaga cagtgaccc 
      ggagattgt gacaacacg tgcttgatt aactaacta 
      aaagtacta ttgtgattt ctattctac aagttattg 
      tatatttta tattgccgc aactaaact agtgcagct 
      gttattatt ttatttgta ctgtagatt gcaatactc 
      ttgagaaac ctaagtttt attgtaagt gttgcgtga 
      aggtttcgg tttgatcca gtgcgagcg agtacaaat 
      ggtctcaaa atttttgtt gatttaata tttttatga 
      ttaggtacg ttatttaaa aacttgtga acagaattg 
      ttatgattt agataaata cagaaggaa tttttactg 
      ccgtgtttt attcgatga aataaaaga aaagttctg 
      actgatg
    • >TC003405 RB
      gtgccccac ttttccccg cgataattt taattgtaa 
      tattgacct ttaaatgtt tgtcgataa ggaagtttc 
      aaagattga gagcaacaa taagccata gaacaaaat 
      ttgaataga tgtgtcggt ggcgacgcg gatttctaa 
      taaaaatat gaagacatc agcgcagaa agcgtcagt 
      gacccaaca cctgttgcg cggtgcaaa ccgcgcgtc 
      cgcgactat attattatt attacaaca aataacgcc 
      gtttaattc cagcagtat tttttaacg caaatctcg 
      tatttttat ttttattta tttattccg gtcttacat 
      tcccacttt tcgcgcaaa atgggccat cacgtgaac 
      ggcttagaa atggaaaag aagaatccc aacgaagaa 
      gagccaccg caaaagatt accaagtag tcttatcgg 
      ttcggttcg ttgtcttcc tgttcagtg aaactgata 
      aaagtgaca acgagcgtt cggtcccaa catttccct 
      cgaaaaaca accggcgga aagttaaaa actgtgctg 
      tgctcgtgt acgtgtcgg agttccgct atcggcaaa 
      cacaaacct ataatgaac gtttgatta tgatttgta 
      ttttcaaat cattccccg atcgtttaa cgatacttt 
      cgtcccgaa aacggaagc ggctcaagt aactcggcc 
      aaatcgcat ccgccattt aaacccgga ttatttttg 
      gcgcctatt tataccgag cccaattat ttatttttt 
      gatttttta cgccgtttt aactcattg agcccagcg 
      aacatgcac ccccatcgc ctctagaac tccgccgaa 
      ttagtttta cccaatttt cgccaaaat gaaaagcaa 
      cgtgacgaa tgggaccag caacaacac cagatatac 
      ggtcgtttt cgaccaaga gaacctcaa caacaccga 
      aaagaaaaa aacaggatg gttcggcac gcgccactt 
      tgtgacctt catgctgtt cctcggaat ggcaaacgc 
      ctatgtaat gaggaccaa catgtctat ggccatcgt 
      ggcaatggt gaaccacac ggcaatcga agcctcaca 
      ttcgaacca cgacgagcc ggttgacac cgaatgcgg 
      cgtggatct gccccaggc gaacaaatc gcaccagga 
      aaacgacgg cgaatttgt ctgggagac ggacatcca 
      gggctacgt cctcagctc cttcttcta cggctacgt 
      cgtcaccca gatcccctt cggcatcct ctccaagcg 
      ctacggcaa catctactt cctgggcgt tggcatgct 
      gatcaactc cctgttcgg cctcctggt gcccgtcgc 
      cgccaacat gggcatctg gtggctgat agccgtgag 
      gttcattca gggcctcgg tgagggccc tattgtccc 
      atgcacaca cgctcttct ggcaaaatg gatccctcc 
      taacgagag aagtcgaat gggtgcttt cgtttatgc 
      aggtatatt tttctgcaa tactgaaaa agagacagt 
      gttacgaat gataggttt tacggcacg ttgagccga 
      aacggtatg ttgtttgat tctgactta gcagtagtt 
      tgaccaagt tcgagtttt gtgtgagtg atgaaggta 
      gaacatcca tcagtgtta ttcaaacat gcggctgag 
      ggtgtccag gtatacagg tgactcatc taacatttt 
      ttcctctcg tttccaaga tttctactc tctttaaac 
      tctttcttg actagcatt acggcaaca gcctgtata 
      tggccaccc cttattttc aaaaaccta ccccagcga 
      attcggtac aattaaaga catttcatt aac
    • >TC003405 RC
      caggtcttg aaaaataca atttttttt gtttcacta 
      ctttatttc agcagagat aatgattgt tgtttaaat 
      ttgcgatcc ctcgtacgt tcttccttg ttgccttta 
      aataactgc aaagtgtta tcagaatcg gtgaaaaaa 
      tgacgaaac aagacgaat acaactttg ttattgcaa 
      ataatatca atgttttga ccggaagac attaaagag 
      aggcgacgt cttaagcgt ggtggtaaa gccttgaaa 
      ctgtttcca gaaattttt tcagactct gtttaattt 
      ataattacg acgataact ttacgtttt aataatttt 
      gttaaatta tgatgataa atttcatgg gttagataa 
      ctgttcctt caaaacaat ttataagaa cgtcattta 
      ctttttaat gtggaatgt ttattttaa acgaatatc 
      ttgtcaatt ataattttt atgaaactt atcaacatg 
      tactttttt atttaattt tgtaacaga gtccacaga 
      gaaatttga ttgttacac taacgcaac atctgccga 
      tttttttga tttcggtca tttttctta aataatttt 
      gcggctatt gacttgcga tacgtatca ctacagatt 
      acgaaaaaa acgacccac tttgtcagc ttgcacaat 
      ttcatcaga actatttac ttagccctg atatcttca 
      ctgtttgta gaaagtctg agattttaa tataattat 
      tgctaacta aaccccaaa ttgtgcgag taaataata 
      aacaaattg ttaagatta tcattctgc gaattaatc 
      cgctcttca aagaagatc acgaagtgt aatcatttg 
      ggcacacgt taattttgt gaaattttg cggccatgt 
      cgtgtttat ttgttcact gggaaaaaa cagtttccg 
      ttcaagtga ataacaact aataataga ttatcacaa 
      tatgtaata ataactgat acaacggat tgtcgatgg 
      aatattcct gcatcaatt ttttattca ataacgctg 
      aaagaaaaa taagcgtgt gcataatta tacaagatg 
      aatctccaa atttcatca aaggaaaac ttatcaccg 
      cctgtatta aaaaatatt tcgcaatag atagtgtta 
      atgcgcaaa aaaactcga aaacttcct tgtctcttt 
      cacattatc ttcggggcg aaagcgagg agtgcacaa 
      gtctgtgaa agaactgga ttttaattc cgaaatatt 
      tttcctagg agcgcaatt tggaacagt catttcaat 
      gccgctgag tggcctttt gtcggcttc gtccgccgg 
      ctggccgtc catttttta cgtttttgg ggccgttgg 
      gaccgtctg gtgcgtggc cttcctgat cttcgtcta 
      cgaagaccc gagcacgaa ccccaaaat gaacatcga 
      cgagaaaaa atacataca aaaagcctt ggggaacgt 
      cagcggggc acctattcc gccaatccc gtggaagtc 
      gatcatcaa atccctgcc cttctgggc cattctgct 
      ggcccatat gggccataa ctacgggta tgagacctt 
      gatgactga gttaccgac gtatatgaa acaagttct 
      acacttcag tattaaaga caatgggtt tctttcggc 
      tctacctta cctagccat gtggttgtt ctcgatctt 
      cattagtca cgttgctga ttggttgtt aaccaaacc 
      gtgcttcac acacactat tgtgcgaaa gttgatcaa 
      cggaattgg ccaatatgg ccctgcgat tgcgttggt 
      ggcggcgtc attcacggg ctgtgaccg atggttgac 
      cgtggccat cctcaccat cggagtggg cctgaacgg 
      cggtatcta ctctggctt caaagtcaa ccacttgga 
      catctcccc gcaattcgc cgggatcct gatggcctt 
      caccaactg tttggcaaa tcttgccgg attgttagc 
      cccaatcta cgctggaaa cgtagtttt gggaacgcc 
      tagtcaagc gaaatggcg cgtcgtctt cgtcacggc 
      agcgggtgt ttatgctgc gtgttgcac gttctatat 
      tctgttcgg ctcagggga gcgccagtc ctgggatca 
      gcaggagga tcccgacaa gaagaagga ggcagacag 
      tgacccgga gattgtgac aacacgtgc ttgattaac 
      taactaaaa gtactattg tgatttcta ttctacaag 
      ttattgtat attttatat tgccgcaac taaactagt 
      gcagctgtt attatttta tttgtactg tagattgca 
      atactcttg agaaaccta agttttatt gtaagtgtt 
      gcgtgaagg tttcggttt gatccagtg cgagcgagt 
      acaaatggt ctcaaaatt tttgttgat ttaatattt 
      ttatgatta ggtacgtta tttaaaaac ttgtgaaca 
      gaattgtta tgatttaga taaatacag aaggaattt 
      ttactgccg tgttttatt cgatgaaat aaaagaaaa 
      gttctgact gatg
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