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Gene information
General information
TC003398 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid15: 62004..66614
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC003398
    iBeetle screen iB_02694
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC003398 RA
      MDNSEVLSD CSESSKASE CGSSNLSTL SGLSEYQGE 
      FLRLKELID PGSSPNVKA SVRRNHITT WYENQIKTL 
      TVQLQNSLK ENENYKNQL YNLEKKKAI LYNDKVETL 
      LKQNNQLEK KLLEANAQI QRLLNVCET KSNTDLQNK 
      MLKLEKNNQ ELSNQNYSL SNELRQCTY QKSEIESDY 
      CNLKSETSD LKLDLVAKL HCIEELKKK ISDQHVELQ 
      NLTQNNVKL VHELDLLTT ELEYVRKSE NWYKHELHQ 
      LQAEKSDLS AQLLQNKKT AASQGQKIQ EMGLELSNL 
      KCVCDELRL ISLKEKERI LKQFENSTD TPIKLENFN 
      KINPPVDID GISTYYETV ISDLTSDVA KIKSDFEHQ 
      QKNFANLSR ENSEVLAKC LLLQNSLNE KENTIEQLE 
      SSKKELKCK LSQAMDKQV EESRKLADL HKNCAKLET 
      ELQLKNEEK DVVEKSVKA IKAQFNSFR CGYQRLKDE 
      LSRKNLEIV RLQRALSEL EENTARDAE HVAKLNERL 
      AFVERANKE AEFAKENYL NEINSLNKA IEGLQTKPE 
      ENNNTSRAV DVMEYICKS CNIGEDGSN RRSVSSGNH 
      SDYIKLLLN RIVAQFQCN ATEIATKDR NIAELRRKI 
      AKLKKFLMN RVEKIEERC RSFSKRSDE NMKMLKALC 
      ENSKGCDQS EKYKHLQAL LQVKIQEEK DKQRRYERN 
      YRTLLRKVR EHMKGRNVA EKHNGYLQE LHNNLSDEC 
      NTLKLQLEM KDCEINKLQ DCVDNYLAI NEKLKQKLE 
      KELTNPCTK CEELEKSNL ADALKRCKC EAKDEVILN 
      LQHENERLL LEMTSLTAK VETLVESNN ALGAKLANL 
      EKLLSEEKA THEITRNGL IQLENTCSK FNNEKEQLG 
      KRLENLSGK NVYLTKELQ HINSCLQQR TEEVNELNL 
      QRKHLENTC LEKEVNLQK LITGLKNQL LVFKNEIDS 
      LKNEKYFLQ RLCNDLKVA LKIHVDHNK ALKEYVNSS 
      FKGKEKFDS MPLLSNFPS PTRYDDGYI NKLLEENKT 
      PVQETPLSD IQDCLNTLR NEICVLQNQ ISERNYL
    CDS sequence
    • >TC003398 RA
      atggataat tccgaagta ttatcagac tgttctgag 
      tcttctaaa gcttcagaa tgtggttct tcaaacctg 
      tcaacactc tcaggcttg agtgaatat caaggtgaa 
      tttttgaga ctaaaggaa cttatcgac cctggaagt 
      tctcccaat gttaaggca agtgtcagg agaaaccat 
      ataacaacg tggtacgaa aaccaaatt aaaactttg 
      actgttcaa ttacaaaat tcactcaag gagaatgaa 
      aattacaaa aaccagtta tacaatttg gagaagaaa 
      aaagctatt ttatacaat gacaaggtc gagacattg 
      ctcaaacaa aacaatcaa ctcgagaaa aagttgtta 
      gaagccaac gcccaaatc cagaggctt cttaacgtc 
      tgtgaaacc aaaagtaac acagacttg cagaacaaa 
      atgttaaag cttgagaaa aacaatcag gagttatcc 
      aaccagaac tattcgcta tcaaatgaa ctacgccaa 
      tgtacttat caaaagagt gagattgaa agtgattat 
      tgtaactta aagtctgaa acaagtgac ttaaaatta 
      gatttggtc gcaaagttg cactgtatc gaagaattg 
      aagaaaaaa atctccgac caacacgtt gagctacaa 
      aacctgacc caaaacaac gtaaagtta gtccacgag 
      cttgattta cttacaacc gagttggaa tacgtccgg 
      aaatcggaa aattggtac aaacacgaa ctgcaccaa 
      ctgcaggcc gagaaaagc gacttgagt gcgcagctt 
      ttgcaaaat aaaaaaacc gcagcctca caagggcaa 
      aagattcaa gaaatgggg ctcgaattg agcaatttg 
      aagtgcgtt tgcgacgag ttgcgcttg atttcgctg 
      aaggaaaag gagaggatt ttgaagcaa tttgaaaac 
      tccactgac acgcctatc aagcttgaa aattttaat 
      aaaattaac cctcccgtc gacattgac ggcattagc 
      acttattat gagacagtt attagcgat ttaacgagc 
      gacgttgct aaaatcaag tcggacttt gagcaccag 
      caaaaaaat ttcgccaac ctttccagg gaaaattcg 
      gaggttttg gcgaaatgt ttgttgttg caaaacagt 
      ttgaacgag aaggaaaac acaatcgag cagttggaa 
      agcagtaaa aaagaattg aaatgcaaa ttatcgcaa 
      gcgatggat aagcaggtg gaggagagc cggaaattg 
      gcggatttg cataaaaac tgcgcgaaa ctcgaaact 
      gagttgcaa ttaaaaaat gaggagaaa gacgttgtt 
      gagaagtcg gtgaaggcg ataaaagcg cagtttaat 
      tcgttcaga tgcggttac cagcggttg aaagacgag 
      ctaagtcgg aagaacctg gaaatagtt cgactgcaa 
      cgtgcgtta agcgaattg gaggaaaac acggcgcgg 
      gatgcagag cacgttgcc aaattgaat gaacggctt 
      gcttttgtg gagagagcg aataaggag gcggaattt 
      gcgaaagaa aattattta aacgagatt aattcgcta 
      aataaagcc attgaaggt ttacaaacg aagccggag 
      gaaaataac aacacgagt cgagctgtt gatgtaatg 
      gaatacatc tgcaaaagc tgtaatatc ggtgaagat 
      ggctctaac cgccgttct gtgtcaagt ggcaatcac 
      tccgactat attaaatta ttactaaat cggattgtt 
      gcacaattt caatgcaac gcgaccgaa attgcgacc 
      aaagaccgc aacatcgcc gaactgagg cgaaaaatc 
      gcaaaattg aaaaagttt ctaatgaat cgcgttgag 
      aaaattgag gagcggtgc agaagtttt tcaaaacgg 
      agcgacgaa aatatgaaa atgttgaaa gcattgtgt 
      gaaaatagc aagggttgc gaccagagc gaaaagtac 
      aagcacttg caagcactg ctacaagtt aaaatccaa 
      gaggagaaa gacaaacaa cgacgatac gagagaaat 
      taccgcaca ttgttacgg aaagttcgc gagcacatg 
      aaaggtaga aatgtagcg gaaaaacat aatggatat 
      ttgcaagaa ctacataac aatttatca gacgagtgt 
      aacacgttg aagttacag ctagagatg aaagattgc 
      gaaataaat aagttacaa gattgtgta gataattat 
      ttggcaatt aatgaaaaa ttaaaacaa aaacttgaa 
      aaggagtta actaatccg tgtaccaaa tgtgaagaa 
      ttggaaaaa agtaacctc gctgatgcg ctaaagagg 
      tgcaaatgc gaggcgaaa gatgaagta attctaaac 
      ttgcaacat gagaatgaa cgtctgtta ttagagatg 
      acttcgctc actgccaag gtggaaaca cttgttgaa 
      agtaataac gcattgggt gccaaatta gctaactta 
      gaaaaattg ttatcggaa gagaaagct acacacgaa 
      attacgcga aatggatta attcagttg gaaaacact 
      tgttcgaaa tttaataat gaaaaggag cagctgggt 
      aaaagactc gagaatctt tcggggaaa aatgtttac 
      ctaactaaa gaactacaa cacattaat tcgtgtctt 
      cagcaaagg actgaagag gtaaacgaa ttaaacttg 
      cagagaaaa cacctcgaa aacacttgc ttggagaag 
      gaagttaat ttgcaaaag cttattact ggtctgaag 
      aaccaactt cttgttttt aaaaatgaa attgactca 
      ctaaagaat gaaaaatac tttttacaa aggctgtgt 
      aacgactta aaagtcgct ttgaaaatt cacgtggat 
      cacaataag gctctgaaa gagtatgtt aatagcagc 
      tttaagggg aaagaaaag tttgacagc atgcctctt 
      ctatcaaat tttcccagt cctacaagg tacgacgat 
      ggttacatt aataaatta ttagaagag aataaaacg 
      cccgttcaa gagaccccg cttagtgat atccaagat 
      tgcctgaat acgcttcga aatgaaatt tgtgtttta 
      cagaatcag atttctgaa agaaattat ttgtga
    mRNA sequence
    • >TC003398 RA
      gtgtttatt taatgatta agtttttaa tcttgaaaa 
      tgaaatgta ccggagtta tgggggact tattttctt 
      ccgtccaca ccgttcgct ttgtcctga aagtttttg 
      catcaaaat aacaagcaa aatattgat agtgaagcc 
      gtatggata attccgaag tattatcag actgttctg 
      agtcttcta aagcttcag aatgtggtt cttcaaacc 
      tgtcaacac tctcaggct tgagtgaat atcaaggtg 
      aatttttga gactaaagg aacttatcg accctggaa 
      gttctccca atgttaagg caagtgtca ggagaaacc 
      atataacaa cgtggtacg aaaaccaaa ttaaaactt 
      tgactgttc aattacaaa attcactca aggagaatg 
      aaaattaca aaaaccagt tatacaatt tggagaaga 
      aaaaagcta ttttataca atgacaagg tcgagacat 
      tgctcaaac aaaacaatc aactcgaga aaaagttgt 
      tagaagcca acgcccaaa tccagaggc ttcttaacg 
      tctgtgaaa ccaaaagta acacagact tgcagaaca 
      aaatgttaa agcttgaga aaaacaatc aggagttat 
      ccaaccaga actattcgc tatcaaatg aactacgcc 
      aatgtactt atcaaaaga gtgagattg aaagtgatt 
      attgtaact taaagtctg aaacaagtg acttaaaat 
      tagatttgg tcgcaaagt tgcactgta tcgaagaat 
      tgaagaaaa aaatctccg accaacacg ttgagctac 
      aaaacctga cccaaaaca acgtaaagt tagtccacg 
      agcttgatt tacttacaa ccgagttgg aatacgtcc 
      ggaaatcgg aaaattggt acaaacacg aactgcacc 
      aactgcagg ccgagaaaa gcgacttga gtgcgcagc 
      ttttgcaaa ataaaaaaa ccgcagcct cacaagggc 
      aaaagattc aagaaatgg ggctcgaat tgagcaatt 
      tgaagtgcg tttgcgacg agttgcgct tgatttcgc 
      tgaaggaaa aggagagga ttttgaagc aatttgaaa 
      actccactg acacgccta tcaagcttg aaaatttta 
      ataaaatta accctcccg tcgacattg acggcatta 
      gcacttatt atgagacag ttattagcg atttaacga 
      gcgacgttg ctaaaatca agtcggact ttgagcacc 
      agcaaaaaa atttcgcca acctttcca gggaaaatt 
      cggaggttt tggcgaaat gtttgttgt tgcaaaaca 
      gtttgaacg agaaggaaa acacaatcg agcagttgg 
      aaagcagta aaaaagaat tgaaatgca aattatcgc 
      aagcgatgg ataagcagg tggaggaga gccggaaat 
      tggcggatt tgcataaaa actgcgcga aactcgaaa 
      ctgagttgc aattaaaaa atgaggaga aagacgttg 
      ttgagaagt cggtgaagg cgataaaag cgcagttta 
      attcgttca gatgcggtt accagcggt tgaaagacg 
      agctaagtc ggaagaacc tggaaatag ttcgactgc 
      aacgtgcgt taagcgaat tggaggaaa acacggcgc 
      gggatgcag agcacgttg ccaaattga atgaacggc 
      ttgcttttg tggagagag cgaataagg aggcggaat 
      ttgcgaaag aaaattatt taaacgaga ttaattcgc 
      taaataaag ccattgaag gtttacaaa cgaagccgg 
      aggaaaata acaacacga gtcgagctg ttgatgtaa 
      tggaataca tctgcaaaa gctgtaata tcggtgaag 
      atggctcta accgccgtt ctgtgtcaa gtggcaatc 
      actccgact atattaaat tattactaa atcggattg 
      ttgcacaat ttcaatgca acgcgaccg aaattgcga 
      ccaaagacc gcaacatcg ccgaactga ggcgaaaaa 
      tcgcaaaat tgaaaaagt ttctaatga atcgcgttg 
      agaaaattg aggagcggt gcagaagtt tttcaaaac 
      ggagcgacg aaaatatga aaatgttga aagcattgt 
      gtgaaaata gcaagggtt gcgaccaga gcgaaaagt 
      acaagcact tgcaagcac tgctacaag ttaaaatcc 
      aagaggaga aagacaaac aacgacgat acgagagaa 
      attaccgca cattgttac ggaaagttc gcgagcaca 
      tgaaaggta gaaatgtag cggaaaaac ataatggat 
      atttgcaag aactacata acaatttat cagacgagt 
      gtaacacgt tgaagttac agctagaga tgaaagatt 
      gcgaaataa ataagttac aagattgtg tagataatt 
      atttggcaa ttaatgaaa aattaaaac aaaaacttg 
      aaaaggagt taactaatc cgtgtacca aatgtgaag 
      aattggaaa aaagtaacc tcgctgatg cgctaaaga 
      ggtgcaaat gcgaggcga aagatgaag taattctaa 
      acttgcaac atgagaatg aacgtctgt tattagaga 
      tgacttcgc tcactgcca aggtggaaa cacttgttg 
      aaagtaata acgcattgg gtgccaaat tagctaact 
      tagaaaaat tgttatcgg aagagaaag ctacacacg 
      aaattacgc gaaatggat taattcagt tggaaaaca 
      cttgttcga aatttaata atgaaaagg agcagctgg 
      gtaaaagac tcgagaatc tttcgggga aaaatgttt 
      acctaacta aagaactac aacacatta attcgtgtc 
      ttcagcaaa ggactgaag aggtaaacg aattaaact 
      tgcagagaa aacacctcg aaaacactt gcttggaga 
      aggaagtta atttgcaaa agcttatta ctggtctga 
      agaaccaac ttcttgttt ttaaaaatg aaattgact 
      cactaaaga atgaaaaat actttttac aaaggctgt 
      gtaacgact taaaagtcg ctttgaaaa ttcacgtgg 
      atcacaata aggctctga aagagtatg ttaatagca 
      gctttaagg ggaaagaaa agtttgaca gcatgcctc 
      ttctatcaa attttccca gtcctacaa ggtacgacg 
      atggttaca ttaataaat tattagaag agaataaaa 
      cgcccgttc aagagaccc cgcttagtg atatccaag 
      attgcctga atacgcttc gaaatgaaa tttgtgttt 
      tacagaatc agatttctg aaagaaatt atttgtgat 
      gtgcaagta ttaaaccag tatttacaa aaaaataaa 
      gtttttgcg tttat